LOCUS       sequence-44291-e         850 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 850)
  AUTHORS   Rosonina E, Ip JY, Calarco JA, Bakowski MA, Emili A, McCracken S, 
            Tucker P, Ingles CJ, Blencowe BJ
  TITLE     Role for PSF in mediating transcriptional activator-dependent 
            stimulation of pre-mRNA processing in vivo.
  JOURNAL   Mol Cell Biol. 2005 Aug;25(15):6734-46.
  PUBMED    16024807
REFERENCE   2  (bases 1 to 850)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Biol."; date: "2005-08"; volume: "25(15)"; pages: "6734-46"
COMMENT     Sequence Label: Myc-PSF-Delta-RRM1+2
FEATURES             Location/Qualifiers
     source          1..850
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(5..23)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(44..60)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(44..60)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(57..79)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    68..84
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(92..98,99..116,117..122))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    137..158
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(275..292)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(526..545)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tgttccttta gtgagggtta atttcgagct tggcgtaatc atggtcatag ctgtttcctg
       61 tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta
      121 aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg
      181 ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga
      241 gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg
      301 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag
      361 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc
      421 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
      481 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
      541 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
      601 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc
      661 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
      721 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
      781 tatcgccact ggcagcagcc actggtaaca ggattancag agcgaggtat gtaggcggtg
      841 ctacagagtt
//
