LOCUS       sequence-442915-        7196 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression vector for expression of transposase (CMV 
            promoter).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7196)
  AUTHORS   Zhang T, Tan S, Tang N, Li Y, Zhang C, Sun J, Guo Y, Gao H, Cai Y, 
            Sun W, Wang C, Fu L, Ma H, Wu Y, Hu X, Zhang X, Gee P, Yan W, Zhao 
            Y, Chen Q, Guo B, Wang H, Zhang YE
  TITLE     Heterologous survey of 130 DNA transposons in human cells highlights
            their functional divergence and expands the genome engineering 
            toolbox.
  JOURNAL   Cell. 2024 Jul 11;187(14):3741-3760.e30. doi: 
            10.1016/j.cell.2024.05.007. Epub 2024 Jun 5.
  PUBMED    38843831
REFERENCE   2  (bases 1 to 7196)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2024.05.007"; journalName: "Cell"; date: 
            "2024-07-11- 11"; volume: "187"; issue: "14"; pages: "3741-3760.e30"
COMMENT     Sequence Label: pCMV-AgaP12-Tn
FEATURES             Location/Qualifiers
     source          1..7196
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        6..385
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        386..589
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     540..560
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     634..653
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        634..652
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(3389..3407)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(3390..3407)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(3427..3444)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    3433..3657
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      3703..4131
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3790..3809)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4000..4021
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4140..4160)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4145..4474
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4325..4460
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     4387..4406
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     polyA_signal    4640..4761
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4677..4696)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4731..4750
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(4810..4826)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4810..4826)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4823..4845)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4834..4850
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4858..4864,4865..4882,4883..4888))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4903..4924
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5041..5058)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5212..5800)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5292..5311)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5971..6762,6763..6831))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6594..6613
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6832..6936)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(7011..7030)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gcgttgacat tgattattga ctagttatta atagtaatca attacggggt cattagttca
       61 tagcccatat atggagttcc gcgttacata acttacggta aatggcccgc ctggctgacc
      121 gcccaacgac ccccgcccat tgacgtcaat aatgacgtat gttcccatag taacgccaat
      181 agggactttc cattgacgtc aatgggtgga gtatttacgg taaactgccc acttggcagt
      241 acatcaagtg tatcatatgc caagtacgcc ccctattgac gtcaatgacg gtaaatggcc
      301 cgcctggcat tatgcccagt acatgacctt atgggacttt cctacttggc agtacatcta
      361 cgtattagtc atcgctatta ccatggtgat gcggttttgg cagtacatca atgggcgtgg
      421 atagcggttt gactcacggg gatttccaag tctccacccc attgacgtca atgggagttt
      481 gttttggcac caaaatcaac gggactttcc aaaatgtcgt aacaactccg ccccattgac
      541 gcaaatgggc ggtaggcgtg tacggtggga ggtctatata agcagagctc tctggctaac
      601 tagagaaccc actgcttact ggcttatcga aattaatacg actcactata gggagaccca
      661 agcttggtac cgagctcgga tccactagta acggccgcca gtgtgctgga attcgccgcc
      721 accatgcctg tgccttgtag cgttagcctg tgtggcaaca accccagaaa cgtgaagaag
      781 agagccctgg agatcagctt ccacatcttc cccaactacg accctctgag acacgcttgg
      841 gtgcagttct gcaacagaga ggagaactgg gagcccagca agagggacgt gatctgcagc
      901 gcccacttcc aggaaagcga cttccagatg cccgaaagcg gaattgtggg cggaaaggtg
      961 ctgagaagaa ccctgttccc caacggcaaa ttcctgctgg cctacttccc taacgacgac
     1021 accaccaaca tcctgtgctt ctgctggctt ctggccattc ccagcattca cagcgttctg
     1081 agcagcgaga tcgagaacag agaggaggtg agcagcgaaa acttcgtgcc cagcacaagc
     1141 gatactctgc tggtgagcga cgtgagcaac atcccccaga ataccagcac cgccttcctg
     1201 aaggagaaca tcaagctgag agaggccaac aagagactga aggccaccct gagccatatt
     1261 gccagcgaga actaccagct gaagaagaag gtgcaggacc tggagaagga gctgaacagc
     1321 atcaagaaca gcagcatccc tcccagcgac ctgattccca agatgaagaa catgctgaag
     1381 agcaccctga ccagcaatca ggtggacctg atcatcggcg agaagaagag agtgagatgg
     1441 accaaggagg agatcagcag agccctgacc ctgagatact tcggcaagaa ggcctacgac
     1501 tacgtgaagg acgacctgaa gatctacctg cctagcccta gcaccctgca gaaatacgcc
     1561 agaaccttca agctgagaga gggcatcctg gaagacgtgc tggtgttcat gggcaacttc
     1621 gtgagcagcc tgagcagcag ggacgctgag tgcatcctga gcttcgacga gatgaagatc
     1681 aagaacgtga tggagtacga ccctagcgcc gacgaagtga ttggacccta caactacatc
     1741 caggtggtga tggccagagc cctgttcaag aactggaagc agcccatcta catcggcttc
     1801 gacaagaaga tgaccaagga gatcctgatg aacatcatca tcaagctgga cgagaagaag
     1861 atcaacgtgg ccggcatcgt gtctgataac tgcagcagca acatcagctg ctggagagat
     1921 ctgggagccc acgattacac caagccctac ttcgagcacc ccatcaccaa gaagaacatc
     1981 tacgtgagcc ctgacgctcc tcacctgctg aaactgctga gaaactggtt catcgaccac
     2041 ggcttcgtgt tcaacggcac catcgtgaca gctcagcccc tgagagatct ggttgagggc
     2101 agactgggag ctgaaatcac ccccctgttc aagctgaata ccggccacct ggaactttct
     2161 agccaggaga gacagaacgt gagaagagct gccgagctgc tgagcagaac aaccgctgtg
     2221 agcctgagaa gatacatgcc caaggagaag gagctggccg acatcatcga gatggtggac
     2281 ctgtggttca gcgtgagcaa cagctttcac cccaacgcca agctgcacta caagagaagc
     2341 tacaccgcca gcgaggacca acttaaggct ctggacgaca tgttcaactt catcgccaac
     2401 atgatcgtgg tgggcaagaa gaacatgcag gtgttccaga agagcatcct gatgcagatc
     2461 aacagcctga agatgctgtt cgtggacatg aaggccaagc acgacatcaa ctacctgagc
     2521 acccacaagc tgaaccagga cctgctggag aacttcttca gccagctgag acaaaagggc
     2581 ggcacctatg atcatcctag ccccctgaac tgcatctaca gaatcagact gatgatcctg
     2641 ggcaaaagcc ccagcgtgct gaaaagcgtg accgacaacg agaacaacaa gaagaacatc
     2701 gagcccgacg tgtttgttac aagcgccgac ttcgagaatg agacagacca gctgaacgag
     2761 atctgcgtga gccaggagca ttatgtgagc gccaccatct ttcatgaggc cgagattgtg
     2821 cctagcctgc tggactttga cgctacagac gccgacgatg agttcagcgc caccaacttc
     2881 gacagcctgt tcatcctgaa cgagcaggag aaagacggcc tggagtacat catcggctac
     2941 atcggcagaa agttcagaga caagtacccc tacctgggcc agtatacctg caacctgacc
     3001 gaggaccact gttacagcca gcctcagagc tacgtggagc acatttctgc tggcggcctg
     3061 tataaaccta gcgccagctt cctggatcag ggcctgaaga tggaggagat cttccagaga
     3121 acccacaagg acggcaagct gatccagagc aagagaatcg tgaacaccct gaccgacctg
     3181 ctgcaaaccc acttccccga tttccccatc gagatcctga agaccttcgc caagctgaga
     3241 atcatcatga gaatgcggtt cttcaacatc aagaaggagg agatcagaag aaacaagaag
     3301 agaaagaccc ccatggagag acaagccgcc aagaagttca gaagaatcgt gaacgcggcc
     3361 gctcgagcat gcatctagag ggccctattc tatagtgtca cctaaatgct agagctcgct
     3421 gatcagcctc gactgtgcct tctagttgcc agccatctgt tgtttgcccc tcccccgtgc
     3481 cttccttgac cctggaaggt gccactccca ctgtcctttc ctaataaaat gaggaaattg
     3541 catcgcattg tctgagtagg tgtcattcta ttctgggggg tggggtgggg caggacagca
     3601 agggggagga ttgggaagac aatagcaggc atgctgggga tgcggtgggc tctatggctt
     3661 ctgaggcgga aagaaccagc tggggctcta gggggtatcc ccacgcgccc tgtagcggcg
     3721 cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc
     3781 tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc
     3841 gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg
     3901 accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg
     3961 tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg
     4021 gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt
     4081 cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat taattctgtg
     4141 gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca
     4201 aagcatgcat ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg
     4261 cagaagtatg caaagcatgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc
     4321 gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat
     4381 tttttttatt tatgcagagg ccgaggccgc ctctgcctct gagctattcc agaagtagtg
     4441 aggaggcttt tttggaggcc taggcttttg caaaaagctc cccgaaatga ccgaccaagc
     4501 gacgcccaac ctgccatcac gagatttcga ttccaccgcc gccttctatg aaaggttggg
     4561 cttcggaatc gttttccggg acgccggctg gatgatcctc cagcgcgggg atctcatgct
     4621 ggagttcttc gcccacccca acttgtttat tgcagcttat aatggttaca aataaagcaa
     4681 tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc
     4741 caaactcatc aatgtatctt atcatgtctg tataccgtcg acctctagct agagcttggc
     4801 gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa
     4861 catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac
     4921 attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca
     4981 ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc
     5041 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc
     5101 aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc
     5161 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag
     5221 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc
     5281 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt
     5341 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct
     5401 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg
     5461 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct
     5521 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat
     5581 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg
     5641 ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa
     5701 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt
     5761 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc
     5821 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt
     5881 atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta
     5941 aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat
     6001 ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac
     6061 tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg
     6121 ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag
     6181 tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt
     6241 aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt
     6301 gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt
     6361 tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt
     6421 cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct
     6481 tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt
     6541 ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac
     6601 cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa
     6661 actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa
     6721 ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca
     6781 aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct
     6841 ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga
     6901 atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc
     6961 tgacgtcgac ggatcgggag atctcccgat cccctatggt gcactctcag tacaatctgc
     7021 tctgatgccg catagttaag ccagtatctg ctccctgctt gtgtgttgga ggtcgctgag
     7081 tagtgcgcga gcaaaattta agctacaaca aggcaaggct tgaccgacaa ttgcatgaag
     7141 aatctgctta gggttaggcg ttttgcgctg cttcgcgatg tacgggccag atatac
//
