LOCUS       sequence-44294-e         765 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 765)
  AUTHORS   Rosonina E, Ip JY, Calarco JA, Bakowski MA, Emili A, McCracken S, 
            Tucker P, Ingles CJ, Blencowe BJ
  TITLE     Role for PSF in mediating transcriptional activator-dependent 
            stimulation of pre-mRNA processing in vivo.
  JOURNAL   Mol Cell Biol. 2005 Aug;25(15):6734-46.
  PUBMED    16024807
REFERENCE   2  (bases 1 to 765)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Biol."; date: "2005-08"; volume: "25(15)"; pages: "6734-46"
COMMENT     Sequence Label: Myc-PSF-Delta-RRM2
FEATURES             Location/Qualifiers
     source          1..765
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(38..54)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(38..54)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(51..73)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    62..78
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(86..92,93..110,111..116))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    131..152
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(269..286)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(520..539)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tttagtgagg gttaatttcg agcttggcgt aatcatggtc atagctgttt cctgtgtgaa
       61 attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct
      121 ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc
      181 agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg
      241 gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc
      301 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag
      361 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
      421 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
      481 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
      541 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
      601 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt
      661 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
      721 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaa
//
