LOCUS       sequence-442955-        7202 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression vector for expression of transposase (CMV 
            promoter).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7202)
  AUTHORS   Zhang T, Tan S, Tang N, Li Y, Zhang C, Sun J, Guo Y, Gao H, Cai Y, 
            Sun W, Wang C, Fu L, Ma H, Wu Y, Hu X, Zhang X, Gee P, Yan W, Zhao 
            Y, Chen Q, Guo B, Wang H, Zhang YE
  TITLE     Heterologous survey of 130 DNA transposons in human cells highlights
            their functional divergence and expands the genome engineering 
            toolbox.
  JOURNAL   Cell. 2024 Jul 11;187(14):3741-3760.e30. doi: 
            10.1016/j.cell.2024.05.007. Epub 2024 Jun 5.
  PUBMED    38843831
REFERENCE   2  (bases 1 to 7202)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2024.05.007"; journalName: "Cell"; date: 
            "2024-07-11- 11"; volume: "187"; issue: "14"; pages: "3741-3760.e30"
COMMENT     Sequence Label: pCMV-P3_AG-Tn
FEATURES             Location/Qualifiers
     source          1..7202
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     629..648
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        629..647
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(3390..3408)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(3391..3408)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(3428..3445)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    3434..3658
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      3704..4132
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3791..3810)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4001..4022
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4141..4161)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4146..4475
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4326..4461
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     4388..4407
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     polyA_signal    4641..4762
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4678..4697)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4732..4751
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(4811..4827)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4811..4827)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4824..4846)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4835..4851
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4859..4865,4866..4883,4884..4889))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4904..4925
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5042..5059)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5213..5801)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5293..5312)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5972..6763,6764..6832))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6595..6614
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6833..6937)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(7012..7031)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctctctgg ctaactagag
      601 aacccactgc ttactggctt atcgaaatta atacgactca ctatagggag acccaagctt
      661 ggtaccgagc tcggatccac tagtaacggc cgccagtgtg ctggaattcg ccgccaccat
      721 gcctagatct tgtgccgccg ccttctgcaa aaacaacgcc gagaacgtga agaagagagg
      781 cctgaacatc accttccaca gcttccccag cgatgatagc ctgcccaagt ggatcgactt
      841 ctgcaagagg gacgagcact ggaagcccac caagatcagc acagtgtgca gcctgcactt
      901 taagcccgac gactaccaga tggccaagag cagcctgcct caaacactcc ccgtgctgaa
      961 gagacttaag ccctacgcca tcccttctct gattcagccc gctgacttca tccaaaacga
     1021 gcccagcaac atgacagccc ccctgaagga atgtaaccag cccaacgtgg agttccaaac
     1081 cgacagcgag tacttcagcg acgtggagaa cgttcctagc cagaccatca tcgacatgaa
     1141 gagagagctg gaccaggtga aggaggacaa cagaaagctg atcgaggtga acaccaacct
     1201 gagagacaag ctgcacagct acttcaacga gaacaagaga ctgaaggccg agatcgacaa
     1261 cctgcagaag cacatcagca agaaggacgc cggcatcgat gaagctgctc tggtgacagc
     1321 catgaaggag agactgaagc ccaccctgag cgagaaccag atcgacatca tcctgaagaa
     1381 gaagaagaga gtggtgtgga ccaaggagga gattggaagc gccctgaccc ttaagtactt
     1441 cggcctgaga tgctacaagt acctggccaa ggacagaaag tttcccctgc ccgctgatgc
     1501 taccctgaag agatacacca agaacctggt ggtgaaggag ggaatcctgg acgacgtgct
     1561 gaagctgatc agcaacctga ccagcacctt caccgagaag gacagacttt gcgccctgag
     1621 cttcgacgag atgaaggtga acagaatcat cgagctggac aaggccagcg acgagattat
     1681 tggcccccac aactatctgc aggtggtgat ggctagaggc ctgtgcaaca agtggaagca
     1741 gcccgtgtac atcggcttcg acaagaagat gaccaaggag atcctgctga agatcatcga
     1801 gaagctgagc gagatcaaca tcaacgtggt ggccatcatc agcgacaact gcagcaccaa
     1861 cgtgagctgc tggaaagaac tgggcgccaa ggattatgag aggccctact tccagcatcc
     1921 caccaccctg aacaacgtgt acgtgatccc tgacgctcct caccttctga agctgctgag
     1981 aaactggttc ctggacagcg gcttcaccta caacggcaag cacatcaagg ccgacctgct
     2041 gttcgatatg atcgccagca gaaacgagac agagatcacc cccctgtaca agctgagcaa
     2101 gacccacctg gtgatgaccc ctcaggagag acagaacgtg agaagagccg ctcagctgct
     2161 gtctcatacc acagctatca gcctgagaag atacttcaag aacaacgccg aggctacaga
     2221 cctggccaac ttcatcgaga aggtggacct gtggttcagc atcagcaaca gctacagccc
     2281 cttcgccaag ctggactaca agaagagcta caccgccagc gacgaccaaa tcaaagccct
     2341 ggacgagatg ttcgagatcg tgagcaacat gaccgtgatc ggcaagcaca gcctgcagat
     2401 cttccagaag agcctgctga tgcagatcac cagcctgaag ctgctgtacg acgacctgca
     2461 caagagacac aacatcagct tcatcagcac ccacaagctg aaccaggacg tgctggagaa
     2521 cttcttcagc cagctgagac agatcggagg cgtgtacgat caccctagcc ctatgagctg
     2581 catccacaga atcaagatga tcatcctggg caaggcccct accttcctga agaaccagac
     2641 cgacctggag cctagcacat tcagctgcac cgacgagtac atcagcagcc agatcagaac
     2701 cagcatcgag atcgagaacg agggaagcga gcaggctaat gacggcgaca tcatcagcgc
     2761 cagcatcatc acaagcgccc tgaatcagcc ccctgtgaag caaagcattc agagcgacac
     2821 cctgtctagc agaagcagcg agatgctgag cagcgttagc agcagcgcta ttgagctgcc
     2881 tgaacaagac agcgacggac tggagtacat catgggctac atcggcagac agtgcttcga
     2941 gaagttcccc cacctgaacc tgggaaacct gagcctgaac ctgaacagcg accacagcta
     3001 tagccaccct cccagctttg tgaagcatct gagcgtggcc ggactttttg ttcctagcga
     3061 ggcctttctg aagcagggct acaagatgga gaagatcttc cagaagctgc accccaacgg
     3121 caacttcaac aagaagaggt acatcagcaa gagactggtg aagagactgc agaaggagtt
     3181 ccctgagctg cctctgattg tggtgcaaca gttcgccaag cacagaatca acatcagaat
     3241 caagttcctg aacatgaaga tcgccaacga gaagagagtg aacaacaaga gaaaggcccc
     3301 cagccatagc aaaaccgcca agaagtgcga gaagctgcag atcagctgct tcgtggcggc
     3361 cgctcgagca tgcatctaga gggccctatt ctatagtgtc acctaaatgc tagagctcgc
     3421 tgatcagcct cgactgtgcc ttctagttgc cagccatctg ttgtttgccc ctcccccgtg
     3481 ccttccttga ccctggaagg tgccactccc actgtccttt cctaataaaa tgaggaaatt
     3541 gcatcgcatt gtctgagtag gtgtcattct attctggggg gtggggtggg gcaggacagc
     3601 aagggggagg attgggaaga caatagcagg catgctgggg atgcggtggg ctctatggct
     3661 tctgaggcgg aaagaaccag ctggggctct agggggtatc cccacgcgcc ctgtagcggc
     3721 gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc
     3781 ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc
     3841 cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc
     3901 gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg
     3961 gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact
     4021 ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt
     4081 tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttaattctgt
     4141 ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc
     4201 aaagcatgca tctcaattag tcagcaacca ggtgtggaaa gtccccaggc tccccagcag
     4261 gcagaagtat gcaaagcatg catctcaatt agtcagcaac catagtcccg cccctaactc
     4321 cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa
     4381 ttttttttat ttatgcagag gccgaggccg cctctgcctc tgagctattc cagaagtagt
     4441 gaggaggctt ttttggaggc ctaggctttt gcaaaaagct ccccgaaatg accgaccaag
     4501 cgacgcccaa cctgccatca cgagatttcg attccaccgc cgccttctat gaaaggttgg
     4561 gcttcggaat cgttttccgg gacgccggct ggatgatcct ccagcgcggg gatctcatgc
     4621 tggagttctt cgcccacccc aacttgttta ttgcagctta taatggttac aaataaagca
     4681 atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt
     4741 ccaaactcat caatgtatct tatcatgtct gtataccgtc gacctctagc tagagcttgg
     4801 cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca
     4861 acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca
     4921 cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc
     4981 attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt
     5041 cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact
     5101 caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag
     5161 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata
     5221 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc
     5281 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
     5341 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
     5401 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg
     5461 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc
     5521 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga
     5581 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg
     5641 gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa
     5701 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg
     5761 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt
     5821 ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat
     5881 tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct
     5941 aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta
     6001 tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa
     6061 ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac
     6121 gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa
     6181 gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag
     6241 taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg
     6301 tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag
     6361 ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg
     6421 tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc
     6481 ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat
     6541 tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata
     6601 ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa
     6661 aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca
     6721 actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc
     6781 aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc
     6841 tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg
     6901 aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac
     6961 ctgacgtcga cggatcggga gatctcccga tcccctatgg tgcactctca gtacaatctg
     7021 ctctgatgcc gcatagttaa gccagtatct gctccctgct tgtgtgttgg aggtcgctga
     7081 gtagtgcgcg agcaaaattt aagctacaac aaggcaaggc ttgaccgaca attgcatgaa
     7141 gaatctgctt agggttaggc gttttgcgct gcttcgcgat gtacgggcca gatatacgcg
     7201 tt
//
