LOCUS       sequence-442979-        8762 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Control for pTet-Gli2shR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8762)
  AUTHORS   Zhu H, Xia L, Shen Q, Zhao M, Gu X, Bouamar H, Wang B, Sun LZ, Zhu X
  TITLE     Differential effects of GLI2 and GLI3 in regulating cervical cancer 
            malignancy in vitro and in vivo.
  JOURNAL   Lab Invest. 2018 Nov;98(11):1384-1396. doi: 
            10.1038/s41374-018-0089-5. Epub 2018 Jul 2.
  PUBMED    29967343
REFERENCE   2  (bases 1 to 8762)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41374-018-0089-5"; journalName: "Lab Invest"; date: 
            "2018-11"; volume: "98"; issue: "11"; pages: "1384-1396"
COMMENT     Sequence Label: pTet-ctl
FEATURES             Location/Qualifiers
     source          1..8762
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(12..31)
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     primer_bind     241..258
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     CDS             complement(460..1080)
                     /codon_start=1
                     /gene="tetR from transposon Tn10"
                     /product="tetracycline repressor TetR"
                     /label=TetR
                     /note="TetR binds to the tetracycline operator tetO to 
                     inhibit transcription. This inhibition can be relieved by 
                     adding tetracycline or doxycycline."
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     primer_bind     988..1007
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
     promoter        complement(1091..1109)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1137..1156)
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     intron          1164..1736
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     promoter        complement(1745..2255)
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     complement(1808..1826)
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     misc_feature    2304..2421
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        complement(2468..2562)
                     /label=H1-2O2 promoter
                     /note="doxycycline-inducible variant of the human H1 RNA 
                     promoter (Henriksen et al., 2007)"
     protein_bind    2468..2486
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2496..2514
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     complement(2532..2551)
                     /label=H1
                     /note="Human H1 promoter, forward primer"
     CDS             complement(2851..2895)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    3080..3313
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    3806..3931
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             3978..4158
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(4159..4385)
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     primer_bind     complement(4413..4433)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(4413..4431)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(4452..4468)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4452..4468)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4465..4487)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4476..4492
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4500..4506,4507..4524,4525..4530))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4545..4566
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(4683..4700)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4854..5442)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4934..4953)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5613..6404,6405..6473))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6236..6255
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6474..6578)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(6604..7059)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6741..6762)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     6953..6972
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7186..7208
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     7200..7217
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     7201..7217
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        7227..7245
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      7266..7401
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    7441..7562
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7452..7471)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     7506..7525
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     LTR             7634..7867
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     CDS             complement(7995..8594)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(8078..8098)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     8575..8594
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     misc_feature    join(8614..8762,1..425)
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
ORIGIN
        1 gccccttgtt gaatacgctt gaggagagcc atttgactct ttccacaact atccaactca
       61 caacgtggca ctggggttgt gccgcctttg caggtgtatc ttatacacgt ggcttttggc
      121 cgcagaggca cctgtcgcca ggtggggggt tccgctgcct gcaaagggtc gctacagacg
      181 ttgtttgtct tcaagaagct tccagaggaa ctgcttcctt cacgacattc aacagacctt
      241 gcattccttt ggcgagaggg gaaagacccc taggaatgct cgtcaagaag acagggccag
      301 gtttccgggc cctcacattg ccaaaagacg gcaatatggt ggaaaataac atatagacaa
      361 acgcacaccg gccttattcc aagcggcttc ggccagtaac gttagggggg ggggagggag
      421 aggggcagat ccctagaact cccgggagcc gctgtacgcg gacccacttt cacatttaag
      481 ttgtttttct aatccgcata tgatcaattc aaggccgaat aagaaggctg gctctgcacc
      541 ttggtgatca aataattcga tagcttgtcg taataatggc ggcatactat cagtagtagg
      601 tgtttccctt tcttctttag cgacttgatg ctcttgatct tccaatacgc aacctaaagt
      661 aaaatgcccc acagcgctga gtgcatataa tgcattctct agtgaaaaac cttgttggca
      721 taaaaaggct aattgatttt cgagagtttc atactgtttt tctgtaggcc gtgtacctaa
      781 atgtactttt gctccatcgc gatgacttag taaagcacat ctaaaacttt tagcgttatt
      841 acgtaaaaaa tcttgccagc tttccccttc taaagggcaa aagtgagtat ggtgcctatc
      901 taacatctca atggctaagg cgtcgagcaa agcccgctta ttttttacat gccaatacaa
      961 tgtaggctgc tctacaccta gcttctgggc gagtttacgg gttgttaaac cttcgattcc
     1021 gacctcatta agcagctcta atgcgctgtt aatcacttta cttttatcta atctagacat
     1081 atcaattcgc cctatagtga gtcgtattac aattctttgc caaaatgatg agacagcaca
     1141 ataaccagca cgttgcccag gagctgtagg aaaaagaaga aggcatgaac atggttagca
     1201 gaggggcccg gtttggactc agagtatttt atcctcatct caaacagtgt atatcattgt
     1261 aaccataaag agaaaggcag gatgatgacc aggatgtagt tgtttctacc aataagaata
     1321 tttccacgcc agccagaatt tatatgcaga aatattctac cttatcattt aattataaca
     1381 attgttctct aaaactgtgc tgaagtacaa tataatatac cctgattgcc ttgaaaaaaa
     1441 agtgattaga gaaagtactt acaatctgac aaataaacaa aagtgaattt aaaaattcgt
     1501 tacaaatgca agctaaagtt taacgaaaaa gttacagaaa atgaaaagaa aataagagga
     1561 gacaatggtt gtcaacagag tagaaagtga aagaaacaaa attatcatga gggtccatgg
     1621 tgatacaagg gacatcttcc cattctaaac aacaccctga aaactttgcc ccctccatat
     1681 aacatgaatt ttacaatagc gaaaaagaaa gaacaatcaa gggtccccaa actcacagat
     1741 ccccctgggg agagaggtcg gtgattcggt caacgaggga gccgactgcc gacgtgcgct
     1801 ccggaggctt gcagaatgcg gaacaccgcg cgggcaggaa cagggcccac actaccgccc
     1861 cacaccccgc ctcccgcacc gccccttccc ggccgctgct ctcggcgcgc cctgctgagc
     1921 agccgctatt ggccacagcc catcgcggtc ggcgcgctgc cattgctccc tggcgctgtc
     1981 cgtctgcgag ggtactagtg agacgtgcgg cttccgtttg tcacgtccgg cacgccgcga
     2041 accgcaagga accttcccga cttaggggcg gagcaggaag cgtcgccggg gggcccacaa
     2101 gggtagcggc gaagatccgg gtgacgctgc gaacggacgt gaagaatgtg cgagacccag
     2161 ggtcggcgcc gctgcgtttc ccggaaccac gcccagagca gccgcgtccc tgcgcaaacc
     2221 cagggctgcc ttggaaaagg cgcaacccca accccctcga gccgcggcca aagtggatct
     2281 ctgctgtccc tgtaataaac ccgaaaattt tgaatttttg taatttgttt ttgtaattct
     2341 ttagtttgta tgtctgttgc tattatgtct actattcttt cccctgcact gtacccccca
     2401 atcccccctt ttcttttaaa attgtggatg aatactgcca tttgtctcga ggtcgagaat
     2461 tccggtgtct ctatcactga tagggaactt ataagtctct atcactgata gggatttcac
     2521 gtttatggtg atttcccaga acacatagcg acatgcaaat attgcggccg ctcgaggcta
     2581 gtctcgtgat cgataccgtc gagatccgtt cactaatcga atggatctgt ctctgtctct
     2641 ctctccacct tcttcttcta ttccttcggg cctgtcgggt cccctcgggg ttgggaggtg
     2701 ggtctgaaac gataatggtg aatatccctg cctaactcta ttcactatag aaagtacagc
     2761 aaaaactatt cttaaaccta ccaagcctcc tactatcatt atgaataatt ttatatacca
     2821 cagccaattt gttatgttaa accaattcca caaacttgcc catttatcta attccaataa
     2881 ttcttgttca ttcttttctt gctggttttg cgattcttca attaaggagt gtattaagct
     2941 tgtgtaattg ttaatttctc tgtcccactc catccaggtc gtgtgattcc aaatctgttc
     3001 cagagattta ttactccaac tagcattcca aggcacagca gtggtgcaaa tgagttttcc
     3061 agagcaaccc caaatcccca ggagctgttg atcctttagg tatctttcca cagccaggat
     3121 tcttgcctgg agctgcttga tgccccagac tgtgagttgc aacagatgct gttgcgcctc
     3181 aatagccctc agcaaattgt tctgctgctg cactatacca gacaataatt gtctggcctg
     3241 taccgtcagc gtcattgacg ctgcgcccat agtgcttcct gctgctccca agaacccaag
     3301 gaacaaagct cctattccca ctgctctttt ttctctctgc accactcttc tctttgcctt
     3361 ggtgggtgct actcctaatg gttcaatttt tactacttta tatttatata attcacttct
     3421 ccaattgtcc ctcatatctc ctcctccagg tctgaagatc agcggccgct tgctgtgcgg
     3481 tggtcttact tttgttttgc tcttcctcta tcttgtctaa agcttccttg gtgtctttta
     3541 tctctatcct ttgatgcaca caatagaggg ttgctactgt attatataat gatctaagtt
     3601 cttctgatcc tgtctgaagg gatggttgta gctgtcccag tatttgtcta cagccttctg
     3661 atgtttctaa caggccagga ttaactgcga atcgttctag ctccctgctt gcccatacta
     3721 tatgttttaa tttatatttt ttctttcccc ctggccttaa ccgaattttt tcccatcgcg
     3781 atctaattct cccccgctta atactgacgc tctcgcaccc atctctctcc ttctagcctc
     3841 cgctagtcaa aatttttggc gtactcacca gtcgccgccc ctcgcctctt gccgtgcgcg
     3901 cttcagcaag ccgagtcctg cgtcgagaga gctcctctgg tttccctttc gctttcaagt
     3961 ccctgttcgg gcgccactgc tagagatttt ccacactgac taaaagggtc tgagggatct
     4021 ctagttacca gagtcacaca acagacgggc acacactact tgaagcactc aaggcaagct
     4081 ttattgaggc ttaagcagtg ggttccctag ttagccagag agctcccagg ctcagatctg
     4141 gtctaaccag agagacccgt ttatgtatcg agctaggcac ttaaatacaa tatctctgca
     4201 atgcggcaat tcagtggttc gtccaatcca tgtcagaccc gtctgttgcc ttcctaataa
     4261 ggcacgatcg taccacctta cttccaccaa tcggcatgca cggtgctttt tctctccttg
     4321 taaggcatgt tgctaactca tcgttaccat gttgcaagac tacaagagta ttgcataaga
     4381 ctacattaag cttgcagctc cagcttttgt tccctttagt gagggttaat tgcgcgcttg
     4441 gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac
     4501 aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc
     4561 acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg
     4621 cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct
     4681 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac
     4741 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga
     4801 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat
     4861 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
     4921 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
     4981 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
     5041 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
     5101 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
     5161 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
     5221 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
     5281 ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
     5341 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
     5401 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
     5461 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga
     5521 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc
     5581 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct
     5641 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata
     5701 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca
     5761 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga
     5821 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga
     5881 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg
     5941 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga
     6001 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt
     6061 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct
     6121 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca
     6181 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat
     6241 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga
     6301 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc
     6361 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg
     6421 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc
     6481 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt
     6541 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca
     6601 cctaaattgt aagcgttaat attttgttaa aattcgcgtt aaatttttgt taaatcagct
     6661 cattttttaa ccaataggcc gaaatcggca aaatccctta taaatcaaaa gaatagaccg
     6721 agatagggtt gagtgttgtt ccagtttgga acaagagtcc actattaaag aacgtggact
     6781 ccaacgtcaa agggcgaaaa accgtctatc agggcgatgg cccactacgt gaaccatcac
     6841 cctaatcaag ttttttgggg tcgaggtgcc gtaaagcact aaatcggaac cctaaaggga
     6901 gcccccgatt tagagcttga cggggaaagc cggcgaacgt ggcgagaaag gaagggaaga
     6961 aagcgaaagg agcgggcgct agggcgctgg caagtgtagc ggtcacgctg cgcgtaacca
     7021 ccacacccgc cgcgcttaat gcgccgctac agggcgcgtc ccattcgcca ttcaggctgc
     7081 gcaactgttg ggaagggcga tcggtgcggg cctcttcgct attacgccag ctggcgaaag
     7141 ggggatgtgc tgcaaggcga ttaagttggg taacgccagg gttttcccag tcacgacgtt
     7201 gtaaaacgac ggccagtgag cgcgcgtaat acgactcact atagggcgaa ttgggtacgt
     7261 ccctaggcct ccaaaaaagc ctcctcacta cttctggaat agctcagagg ccgaggcggc
     7321 ctcggcctct gcataaataa aaaaaattag tcagccatgg ggcggagaat gggcggaact
     7381 gggcggagtt aggggcggga tgggcggagt taggggcggg atagctagag ccagacatga
     7441 taagatacat tgatgagttt ggacaaacca caactagaat gcagtgaaaa aaatgcttta
     7501 tttgtgaaat ttgtgatgct attgctttat ttgtaaccat tataagctgc aataaacaag
     7561 ttcctctcac tctctgatat tcatttcttt gcaagttata aatactgaat aataagatga
     7621 catgaactac tactgctaga gattttccac actgactaaa agggtctgag ggatctctag
     7681 ttaccagagt cacacaacag acgggcacac actacttgaa gcactcaagg caagctttat
     7741 tgaggcttaa gcagtgggtt ccctagttag ccagagagct cccaggctca gatctggtct
     7801 aaccagagag acccagtaca agcaaaaagc agatcttgtc ttcgttggga gtgaattagc
     7861 ccttccagtc cccccttttc ttttaaaaag tggctaagat ctacagctgc cttgtaagtc
     7921 attggtctta aaggtaccga tgcatggggt cgtgcgctcc tttcggtcgg gcgctgcggg
     7981 tcgtggggcg ggcgtcaggc accgggcttg cgggtcatgc accaggtgcg cggtccttcg
     8041 ggcacctcga cgtcggcggt gacggtgaag ccgagccgct cgtagaaggg gaggttgcgg
     8101 ggcgcggagg tctccaggaa ggcgggcacc ccggcgcgct cggccgcctc cactccgggg
     8161 agcacgacgg cgctgcccag acccttgccc tggtggtcgg gcgagacgcc gacggtggcc
     8221 aggaaccacg cgggctcctt gggccggtgc ggcgccagga ggccttccat ctgttgctgc
     8281 gcggccagcc gggaaccgct caactcggcc atgcgcgggc cgatctcggc gaacaccgcc
     8341 cccgcttcga cgctctccgg cgtggtccag accgccaccg cggcgccgtc gtccgcgacc
     8401 cacaccttgc cgatgtcgag cccgacgcgc gtgaggaaga gttcttgcag ctcggtgacc
     8461 cgctcgatgt ggcggtccgg atcgacggtg tggcgcgtgg cggggtagtc ggcgaacgcg
     8521 gcggcgaggg tgcgtacggc cctggggacg tcgtcgcggg tggcgaggcg caccgtgggc
     8581 ttgtactcgg tcatggtaag ctccggtgga tccttatcat cgtgtttttc aaaggaaaac
     8641 cacgtccccg tggttcgggg ggcctagacg tttttttaac ctcgactaaa cacatgtaaa
     8701 gcatgtgcac cgaggcccca gatcagatcc catacaatgg ggtaccttct gggcatcctt
     8761 ca
//
