LOCUS       sequence-442984-        9160 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of destabilized GFP (dGFP) and NanoLuc for shortened in 
            vivo half-life.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9160)
  AUTHORS   Kantor B, Odonovan B, Rittiner J, Hodgson D, Lindner N, Guerrero S, 
            Dong W, Zhang A, Chiba-Falek O
  TITLE     All-in-one AAV-delivered epigenome-editing platform: 
            proof-of-concept and therapeutic implications for neurodegenerative 
            disorders.
  JOURNAL   bioRxiv [Preprint]. 2024 May 19:2023.04.14.536951. doi: 
            10.1101/2023.04.14.536951.
  PUBMED    38798630
REFERENCE   2  (bases 1 to 9160)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            [Preprint]. 2024 May 19:2023.04.14.536951. doi: 
            10.1101/2023.04.14.536951."
COMMENT     Sequence Label: pBK1340-LV-DUAL-dGFP-NanoLuc-Puro
FEATURES             Location/Qualifiers
     source          1..9160
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        21..125
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(126..194,195..986)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(344..363)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1157..1745
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1646..1665
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1899..1916
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2033..2054
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2069..2074,2075..2092,2093..2099)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2107..2123
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2112..2134
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2131..2147
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2131..2147
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     2166..2186
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        2168..2186
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        2214..2440
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             2441..2621
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    2668..2793
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    3286..3519
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             3704..3748
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    4015..4131
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        4154..4457
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4458..4661
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4612..4632
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     4658..4682
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      4755..4764
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(4761..4763,4764..4766,4767..5477,5478..5483,
                     5484..5603)
                     /codon_start=1
                     /product="EGFP destabilized by fusion to residues 422-461 
                     of mouse ornithine decarboxylase, giving an in vivo 
                     half-life of ~2 hours"
                     /label=d2EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYKKLSHGFPPEVEEQDDGTLPMSCAQESGMDRHPAACASARINV
                     "
     primer_bind     complement(4806..4827)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(5067..5086)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5414..5435
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             5628..5684
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     regulatory      5685..5694
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             5691..6206
                     /codon_start=1
                     /product="NanoLuc(R) luciferase"
                     /label=Nluc
                     /note="human codon-optimized"
                     /translation="MVFTLEDFVGDWRQTAGYNLDQVLEQGGVSSLFQNLGVSVTPIQR
                     IVLSGENGLKIDIHVIIPYEGLSGDQMGQIEKIFKVVYPVDDHHFKVILHYGTLVIDGV
                     TPNMIDYFGRPYEGIAVFDGKKITVTGTLWNGNKIIDERLINPDGSLLFRVTINGVTGW
                     RLCERILA"
     primer_bind     complement(6244..6264)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6249..6578
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6429..6564
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             6588..7187
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6588..6607)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     7084..7104
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    7222..7810
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7275..7295)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7693..7704)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7892..8125
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    8197..8318
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8234..8253)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8288..8307
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      8358..8493
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(8513..8532)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8514..8532)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8542..8559)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8542..8558)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8551..8573)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      8700..9155
                     /direction=RIGHT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8787..8806)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8997..9018
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa
       61 atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga
      121 agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc
      181 ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg
      241 gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc
      301 gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat
      361 tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg
      421 acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag
      481 aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa
      541 cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc
      601 gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca
      661 cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc
      721 tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc
      781 tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg
      841 ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta
      901 tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag
      961 gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga
     1021 ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc
     1081 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa
     1141 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
     1201 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
     1261 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt
     1321 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
     1381 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
     1441 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
     1501 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
     1561 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
     1621 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
     1681 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
     1741 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
     1801 acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt
     1861 gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag
     1921 cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca
     1981 gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatgtga
     2041 gttagctcac tcattaggca ccccaggctt tccactttat gcttccggct cgtatgttgt
     2101 gtggaattgt gagcggataa caatttcaca caggaaacag ctatgaccat gattacgcca
     2161 agcgcgcaat taaccctcac taaagggaac aaaagctgga gctgcaagct taatgtagtc
     2221 ttatgcaata ctcttgtagt cttgcaacat ggtaacgatg agttagcaac atgccttaca
     2281 aggagagaaa aagcaccgtg catgccgatt ggtggaagta aggtggtacg atcgtgcctt
     2341 attaggaagg caacagacgg gtctgacatg gattggacga accactgaat tgccgcattg
     2401 cagagatatt gtatttaagt gcctagctcg atacataaac gggtctctct ggttagacca
     2461 gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag
     2521 cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag
     2581 atccctcaga cccttttagt cagtgtggaa aatctctagc agtggcgccc gaacagggac
     2641 ttgaaagcga aagggaaacc agaggagctc tctcgacgca ggactcggct tgctgaagcg
     2701 cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt gactagcgga
     2761 ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag aattagatcg
     2821 cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt aaaacatata
     2881 gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt agaaacatca
     2941 gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg atcagaagaa
     3001 cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag gatagagata
     3061 aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag taagaccacc
     3121 gcacagcaag cggccgctga tcttcagacc tggaggagga gatatgaggg acaattggag
     3181 aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag cacccaccaa
     3241 ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag ctttgttcct
     3301 tgggttcttg ggagcagcag gaagcactat gggcgcagcg tcaatgacgc tgacggtaca
     3361 ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga gggctattga
     3421 ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc aggcaagaat
     3481 cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg gttgctctgg
     3541 aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata aatctctgga
     3601 acagatttgg aatcacacga cctggatgga gtgggacaga gaaattaaca attacacaag
     3661 cttaatacac tccttaattg aagaatcgca aaaccagcaa gaaaagaatg aacaagaatt
     3721 attggaatta gataaatggg caagtttgtg gaattggttt aacataacaa attggctgtg
     3781 gtatataaaa ttattcataa tgatagtagg aggcttggta ggtttaagaa tagtttttgc
     3841 tgtactttct atagtgaata gagttaggca gggatattca ccattatcgt ttcagaccca
     3901 cctcccaacc ccgaggggac ccgacaggcc cgaaggaata gaagaagaag gtggagagag
     3961 agacagagac agatccattc gattagtgaa cggatctcga cggtatcggt taacttttaa
     4021 aagaaaaggg gggattgggg ggtatagtgc aggggaaaga atagtagaca taatagcaac
     4081 agacatacaa actaaagaat tacaaaaaca aattacaaaa ttcaaaattt tatcgataag
     4141 cttgggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
     4201 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
     4261 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
     4321 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
     4381 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
     4441 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
     4501 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
     4561 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
     4621 gcggtaggcg tgtacggtgg gaggtctata taagcagagc tcgtttagtg aaccgtcaga
     4681 tcgcctggag acgccatcca cgctgttttg acctccatag aagacaccga ctctagagga
     4741 tccatccacc ggtcgccacc atggtgagca agggcgagga gctgttcacc ggggtggtgc
     4801 ccatcctggt cgagctggac ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg
     4861 gcgagggcga tgccacctac ggcaagctga ccctgaagtt catctgcacc accggcaagc
     4921 tgcccgtgcc ctggcccacc ctcgtgacca ccctgaccta cggcgtgcag tgcttcagcc
     4981 gctaccccga ccacatgaag cagcacgact tcttcaagtc cgccatgccc gaaggctacg
     5041 tccaggagcg caccatcttc ttcaaggacg acggcaacta caagacccgc gccgaggtga
     5101 agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg
     5161 acggcaacat cctggggcac aagctggagt acaactacaa cagccacaac gtctatatca
     5221 tggccgacaa gcagaagaac ggcatcaagg tgaacttcaa gatccgccac aacatcgagg
     5281 acggcagcgt gcagctcgcc gaccactacc agcagaacac ccccatcggc gacggccccg
     5341 tgctgctgcc cgacaaccac tacctgagca cccagtccgc cctgagcaaa gaccccaacg
     5401 agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc actctcggca
     5461 tggacgagct gtacaagaag cttagccatg gcttcccgcc ggaggtggag gagcaggatg
     5521 atggcacgct gcccatgtct tgtgcccagg agagcgggat ggaccgtcac cctgcagcct
     5581 gtgcttctgc taggatcaat gtggaggagg gcggctcgag gtcgagagcc acgaacttct
     5641 ctctgttaaa gcaagcagga gacgtggaag aaaaccccgg tcccgccacc atggtcttca
     5701 cactcgaaga tttcgttggg gactggcgac agacagccgg ctacaacctg gaccaagtcc
     5761 ttgaacaggg aggtgtgtcc agtttgtttc agaatctcgg ggtgtccgta actccgatcc
     5821 aaaggattgt cctgagcggt gaaaatgggc tgaagatcga catccatgtc atcatcccgt
     5881 atgaaggtct gagcggcgac caaatgggcc agatcgaaaa aatttttaag gtggtgtacc
     5941 ctgtggatga tcatcacttt aaggtgatcc tgcactatgg cacactggta atcgacgggg
     6001 ttacgccgaa catgatcgac tatttcggac ggccgtatga aggcatcgcc gtgttcgacg
     6061 gcaaaaagat cactgtaaca gggaccctgt ggaacggcaa caaaattatc gacgagcgcc
     6121 tgatcaaccc cgacggctcc ctgctgttcc gagtaaccat caacggagtg accggctggc
     6181 ggctgtgcga acgcattctg gcgtaagtcg accatgcatc tagacccagc ggcgcgcccc
     6241 tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg ctccccagca ggcagaagta
     6301 tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag
     6361 caggcagaag tatgcaaagc atgcatctca attagtcagc aaccatagtc ccgcccctaa
     6421 ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc catggctgac
     6481 taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt
     6541 agtgaggagg cttttttgga ggcctaggct tttgcaaaaa gcttaccatg accgagtaca
     6601 agcccacggt gcgcctcgcc acccgcgacg acgtccccag ggccgtacgc accctcgccg
     6661 ccgcgttcgc cgactacccc gccacgcgcc acaccgtcga tccggaccgc cacatcgagc
     6721 gggtcaccga gctgcaagaa ctcttcctca cgcgcgtcgg gctcgacatc ggcaaggtgt
     6781 gggtcgcgga cgacggcgcc gcggtggcgg tctggaccac gccggagagc gtcgaagcgg
     6841 gggcggtgtt cgccgagatc ggcccgcgca tggccgagtt gagcggttcc cggctggccg
     6901 cgcagcaaca gatggaaggc ctcctggcgc cgcaccggcc caaggagccc gcgtggttcc
     6961 tggccaccgt cggcgtctcg cccgaccacc agggcaaggg tctgggcagc gccgtcgtgc
     7021 tccccggagt ggaggcggcc gagcgcgccg gggtgcccgc cttcctggag acctccgcgc
     7081 cccgcaacct ccccttctac gagcggctcg gcttcaccgt caccgccgac gtcgaggtgc
     7141 ccgaaggacc gcgcacctgg tgcatgaccc gcaagcccgg tgcctgacgg gcgcgccgaa
     7201 ttcgatatca agcttatcga taatcaacct ctggattaca aaatttgtga aagattgact
     7261 ggtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg
     7321 tatcatgcta ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggttg
     7381 ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg
     7441 tttgctgacg caacccccac tggttggggc attgccacca cctgtcagct cctttccggg
     7501 actttcgctt tccccctccc tattgccacg gcggaactca tcgccgcctg ccttgcccgc
     7561 tgctggacag gggctcggct gttgggcact gacaattccg tggtgttgtc ggggaaatca
     7621 tcgtcctttc cttggctgct cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc
     7681 tgctacgtcc cttcggccct caatccagcg gaccttcctt cccgcggcct gctgccggct
     7741 ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga gtcggatctc cctttgggcc
     7801 gcctccccgc atcgataccg ggtaccttta agaccaatga cttacaaggc agctgtagat
     7861 cttagccact ttttaaaaga aaagggggga ctggaagggc taattcactc ccaacgaaga
     7921 caagatctgc tttttgcttg tactgggtct ctctggttag accagatctg agcctgggag
     7981 ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt
     8041 caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt
     8101 tagtcagtgt ggaaaatctc tagcagtagt agttcatgtc atcttattat tcagtattta
     8161 taacttgcaa agaaatgaat atcagagagt gagaggaact tgtttattgc agcttataat
     8221 ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat
     8281 tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctggct ctagctatcc
     8341 cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc
     8401 atggctgact aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat
     8461 tccagaagta gtgaggaggc ttttttggag gcctagggac gtacccaatt cgccctatag
     8521 tgagtcgtat tacgcgcgct cactggccgt cgttttacaa cgtcgtgact gggaaaaccc
     8581 tggcgttacc caacttaatc gccttgcagc acatccccct ttcgccagct ggcgtaatag
     8641 cgaagaggcc cgcaccgatc gcccttccca acagttgcgc agcctgaatg gcgaatggga
     8701 cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc
     8761 tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac
     8821 gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag
     8881 tgctttacgg cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc
     8941 atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg
     9001 actcttgttc caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata
     9061 agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa
     9121 cgcgaatttt aacaaaatat taacgcttac aatttaggtg
//
