LOCUS       sequence-442986-       10254 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  T7 protein expression of MBP-His-TEV-ECOR31 Hachiman mutant (HamA 
            E138A, K140A).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10254)
  AUTHORS   Tuck OT, Adler BA, Armbruster EG, Lahiri A, Hu JJ, Zhou J, Pogliano 
            J, Doudna JA
  TITLE     Hachiman is a genome integrity sensor.
  JOURNAL   bioRxiv [Preprint]. 2024 Feb 29:2024.02.29.582594. doi: 
            10.1101/2024.02.29.582594.
  PUBMED    38464307
REFERENCE   2  (bases 1 to 10254)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            [Preprint]. 2024 Feb 29:2024.02.29.582594. doi: 
            10.1101/2024.02.29.582594."
COMMENT     Sequence Label: pBA1583_ECOR31HamA(E138AK140A)B_CoPurification
FEATURES             Location/Qualifiers
     source          1..10254
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     2..21
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2..20
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             109..131
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             151..180
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     CDS             190..1290
                     /codon_start=1
                     /gene="malE (mutated)"
                     /product="maltose binding protein from E. coli"
                     /label=MBP
                     /note="This version of the gene does not encode a signal 
                     sequence, so MBP will remain in the cytosol."
                     /translation="MKIEEGKLVIWINGDKGYNGLAEVGKKFEKDTGIKVTVEHPDKLE
                     EKFPQVAATGDGPDIIFWAHDRFGGYAQSGLLAEITPDKAFQDKLYPFTWDAVRYNGKL
                     IAYPIAVEALSLIYNKDLLPNPPKTWEEIPALDKELKAKGKSALMFNLQEPYFTWPLIA
                     ADGGYAFKYENGKYDIKDVGVDNAGAKAGLTFLVDLIKNKHMNADTDYSIAEAAFNKGE
                     TAMTINGPWAWSNIDTSKVNYGVTVLPTFKGQPSKPFVGVLSAGINAASPNKELAKEFL
                     ENYLLTDEGLEAVNKDKPLGAVALKSYEEELAKDPRIAATMENAQKGEIMPNIPQMSAF
                     WYAVRTAVINAASGRQTVDEALKDAQT"
     primer_bind     1264..1287
                     /label=MBP-F
                     /note="Maltose binding protein, forward primer"
     CDS             1345..1365
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     primer_bind     complement(5785..5803)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      5799..5846
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        complement(join(6212..6217,6218..6234,6235..6240))
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     primer_bind     complement(6267..6285)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6353..6457
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6458..6526,6527..7318)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6676..6695)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7489..8077
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7978..7997
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8231..8248
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    8263..8402
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(8328..8347)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8488..8510
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(8504..8695)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     primer_bind     10153..10172
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 ttaatacgac tcactatagg gagaccacaa cggtttccct ctagtgccgg ctccggagag
       61 ctctttaatt aagcggccgc cctgcaggac tcgagttcta gaaataattt tgtttaactt
      121 taagaaggag atatacatat gaaatcttct caccatcacc atcaccatca ccatcaccat
      181 ggttcttcta tgaaaatcga agaaggtaaa ctggtaatct ggattaacgg cgataaaggc
      241 tataacggtc tcgctgaagt cggtaagaaa ttcgagaaag ataccggaat taaagtcacc
      301 gttgagcatc cggataaact ggaagagaaa ttcccacagg ttgcggcaac tggcgatggc
      361 cctgacatta tcttctgggc acacgaccgc tttggtggct acgctcaatc tggcctgttg
      421 gctgaaatca ccccggacaa agcgttccag gacaagctgt atccgtttac ctgggatgcc
      481 gtacgttaca acggcaagct gattgcttac ccgatcgctg ttgaagcgtt atcgctgatt
      541 tataacaaag atctgctgcc gaacccgcca aaaacctggg aagagatccc ggcgctggat
      601 aaagaactga aagcgaaagg taagagcgcg ctgatgttca acctgcaaga accgtacttc
      661 acctggccgc tgattgctgc tgacgggggt tatgcgttca agtatgaaaa cggcaagtac
      721 gacattaaag acgtgggcgt ggataacgct ggcgcgaaag cgggtctgac cttcctggtt
      781 gacctgatta aaaacaaaca catgaatgca gacaccgatt actccatcgc agaagctgcc
      841 tttaataaag gcgaaacagc gatgaccatc aacggcccgt gggcatggtc caacatcgac
      901 accagcaaag tgaattatgg tgtaacggta ctgccgacct tcaagggtca accatccaaa
      961 ccgttcgttg gcgtgctgag cgcaggtatt aacgccgcca gtccgaacaa agagctggca
     1021 aaagagttcc tcgaaaacta tctgctgact gatgaaggtc tggaagcggt taataaagac
     1081 aaaccgctgg gtgccgtagc gctgaagtct tacgaggaag agttggcgaa agatccacgt
     1141 attgccgcca ctatggaaaa cgcccagaaa ggtgaaatca tgccgaacat cccgcagatg
     1201 tccgctttct ggtatgccgt gcgtactgcg gtgatcaacg ccgccagcgg tcgtcagact
     1261 gtcgatgaag ccctgaaaga cgcgcagact aattcgagct cgaacaacaa caacaataac
     1321 aataacaaca acctcgggat cgaggaaaac ctgtacttcc aatccaatgc aatggcaaat
     1381 ttcgaggact ggtgtgattc gacagagcgt aatatcagcg accattattt gcagtcaata
     1441 acggccaggg atgccgagtg tatgttcggc gttcaggtta tggccgcgct aatacctgaa
     1501 cactatgcgt caccaagaaa tatcgctaac gcatttgaag ctctaggtaa accggggctg
     1561 gcggcataca ttgctggaaa attgcctgaa acgaaacaga ttcgttccgg tgatttgggt
     1621 gagatatttg caactgaatg gataaatgca cgtagcaatg gctataaaac gcctataaaa
     1681 agattgcgct ggaaagatca tcgcaatatg tcgatgcgtg gtgaggacgt cattggtatt
     1741 tacatagatc agtcttctca acaacttttt ttccttaaaa ccgcggcggc gagtcgcgcc
     1801 aaaatgacgg gagaggtggt ttctgaagcc cgcgataact tgaataaaga gcaagggctg
     1861 ccatcttcac atgccctaat gttcatagca gatcgcctga atgaacaggg tgaggagctt
     1921 ttggctaaag cgatcttaaa tgcaacgctt cggcaaggaa ttgttccagg atgcgtccgg
     1981 catcttatct ttctattatc gggcaactct tcagaaacta tgctgaccac ttcaattgaa
     2041 aagtatacag gtcaaaataa tcagtggggt gtttgtctaa gaattgctcg ccatggtgag
     2101 tttattgccg caacttttga aaaggtgatt agcgatgcca gcaacagctg atgaaattat
     2161 cgaagctatt aaggaagctt cagctgtagg ttttcgcggg cgcctgattg cacgcgggca
     2221 agcccggtcg gtgatctggc gagacggtga cttgccgccg gatgcaccgg agtttagcgc
     2281 actgctcagt caggaccttc aaggctatgc ttatgcccta atcgatctcg gccttcgtct
     2341 tcgagaactt aatggagacg acgcctatgc gcgaattgct tttgaacagg ccggaaccgc
     2401 actcgagtcg gctattgcta aaggaaaacg cgacagccgg gatacagatt ttcacttcgt
     2461 aatggctgct gcaagctatc accttgcgca tctttcagca cgagcctatt cgttactcgc
     2521 aatggttggt caggatgata atttttcacc aatagagagg gctcttacac aactgatccg
     2581 gcgtgatttg cgtactctgc gcgataatgc gttgggcttt cggctcagag gtgatggcag
     2641 tgacgtgaaa attactgaaa tcctccaggc tcgcctaaac ttgccgcaag acgagaacgg
     2701 tgacagtgag tcagaggagg acatcctttt tgatgggctt gatttggcac tgactgacgc
     2761 ctatatgtcg gccatttctc tctaccttct cgctgtagaa aggggagaat cacgtttgct
     2821 ttccagggct attgaaaaat taagaattag tctaagtata tgtgctcagt ttaatatgct
     2881 accccagtgg tggctaaact ttattaccat tcatctgtta tctgaccttt ggtcagatac
     2941 attccatgaa agattgccac tggttccagt tggtggtgac gctgctgaat ggcccgcgct
     3001 tcgggaactg tttattgcac tgcttcaaag acgacctcga gccgaaattg atctctggcc
     3061 atcgcagaga gaggccgccg gacgctctgt taatgacaat gacgatttgg ttgtttcgtt
     3121 gccaaccagc gctggaaaaa ctcgtattgc tgaattgtgt attcttcgct gtctggcggg
     3181 tggcaagcgt gttgttttca tcaccccgct tcgtgcgctt tctgcacaaa cagaagccac
     3241 tctctccaga acatttgggc cgcttgggaa aacaatatca atgctgtatg gcagcattgg
     3301 cgtaagtggt atggatgagg atgcaattcg ccagcgtgac atcgtcgtcg ccacgcctga
     3361 aaaacttgac tttgctctcc ggaatgaccc gtcaattatt aatgatgtag gactttttat
     3421 ttttgatgag gggcacatga ttggggccga tgaacgagag gttcggtatg aagttcaaat
     3481 tcaacggctg ttgcgcagac aggatgccga tacgcgcaga attgtctgcc tctcggcaat
     3541 tctacctgat ggtgagcaac tcgatgattt tgccggttgg ctccgtcgtg acaagcccgg
     3601 aggaccaatc aaaaataatt ggcgaccgac ccgcttgcag tttggcgagg tcatatggtc
     3661 cgctccagca ggtcggctta atctcagcgt cggttatgag gcggcttggg tatcgcgttt
     3721 tatcgtcagc agacaaccgc caaaggttaa attacctaat aaaaagcagc gaacaaaaat
     3781 gtttccctct gataacaagg agctctgctt agcgacagcc tggcgtctaa ttgaagatgg
     3841 acagaccgta cttatctact gcccacttcg ccggagcgtc gaaccgtttg ccgagacaat
     3901 tgtcgatctg catcagcgcg gactactgcc atctttattt gatgctgccc cggacattct
     3961 ggatacggct atctcgctgg gagaagaatg gttgggggca cacagcccga tacttgcctg
     4021 cctacggttg ggcgtggctc ttcatcatgg cgcattgcct acggcttacc gcaaagaaat
     4081 cgaacgtttg ctgcgagatg gggtacttaa agtgacgatc tcatcaccga cgctagcaca
     4141 aggtcttaat ctttcggcaa cagctatcgt catgcattct cttcatcgga atcgcgagct
     4201 tatcaaagtg tccgagttca gaaacgtcat aggcagagca gggcgcgcat atgtagatgt
     4261 tgaagggctg gtgatttatc caatattcga taaagtaaat aaacgccaga ctaactggca
     4321 taccctcaca tctgacacgg gggcccgtga gatggagagc ggactaatac agcttgtctg
     4381 tgtgttattg attcgcatgc atacacggct tggtggggat ttaaaagcac tcactgaata
     4441 tgtgaccaac aacgccgtag cgtgggaatt tccagaaatt atgaccgaat ctccgcaaga
     4501 aagagatatt gcgcaggcca tctgggaaaa acaactttca acgcttgata ctgctattct
     4561 gagccttctt ggtgagaatg atatcccgga tgaccagatt gaaactgctc ttgatgacat
     4621 tcttcagtct tcactttggc aacgcagttt gcagcgttat agggatgaga atgaacggat
     4681 tttgctgaaa tcgggactcc tgtcccgcag ccgctacatc tggcagcgct caacggcagc
     4741 agggcgccgg gggtatttcc tgtcgggggt aggacttaca accgggctac gacttgatgc
     4801 tattgccgct aaggccaacc aactattgat tgatgcgaat gcggctataa tgggcggtga
     4861 tgccgaagag gcaattgctg ctatcacggc gctcgctgaa gaggtcttta ccttctatcc
     4921 gtttataccc gatccattgc ctggcgactg gagaggtatt ttacgttcct ggctattggg
     4981 tgaacccatg actaacgtcg cgaatacgca ggcgtctgaa acgcttcaat tcgtcgagaa
     5041 tgggctggtg tatcgacttc cgtgggcaat ggaggctatt agggtacgtg caacagctaa
     5101 tggagacctc ataggtgata cagataccac gctagacgat tatgagttag gttttgcggt
     5161 tgctgctgtt gaaacaggta ccttgagccg ttcgtcttca ctgcttattc aggcgggttt
     5221 tagctctagg cttgcagcca taaaagtggt tacagacact actgccgatt ttcaatccgg
     5281 ccaggagctt agaaggtggc tgaactctga agaagtgata agccacacgg ataatcatga
     5341 ctggcctaca cctgaaacca gagtgatgtg gctggaattc ttgggtagtc tcagccccaa
     5401 aggcagccag gtttggagtc gtcatcgtta taacggaatg gtggactgga gagatactcc
     5461 ggcagtgatc ggcacaccat tgcagcttta cactgttgat ggtattcatc acgtactggc
     5521 tgatgatggc actcctctgg gaagtataaa tggcagaatt aacacaaaca gaagaggtct
     5581 tcttagagtt gaggtggatg atgagaatgg tcgtgccatg ttcgattatt taggaccgga
     5641 cgactttatt tctacctaat aacattggaa gtggataacg gatccgcgat cgcggcgcgc
     5701 cacctggtgg ccggccggta ccacgcgtgc gcgctgatcc ggctgctaac aaagcccgaa
     5761 aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct
     5821 ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg
     5881 acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg
     5941 actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc
     6001 atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg
     6061 atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg
     6121 gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg
     6181 cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa
     6241 acatgagaat tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt
     6301 catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac
     6361 ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc
     6421 ctgataaatg cttcaataac attgaaaaag gaagagtatg agtattcaac atttccgtgt
     6481 cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct
     6541 ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga
     6601 tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag
     6661 cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca
     6721 actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga
     6781 aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag
     6841 tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc
     6901 ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa
     6961 tgaagccata ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg caacaacgtt
     7021 gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg
     7081 gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt
     7141 tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg
     7201 gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat
     7261 ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact
     7321 gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa
     7381 aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt
     7441 ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt
     7501 ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg
     7561 tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca
     7621 gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt
     7681 agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga
     7741 taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc
     7801 gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact
     7861 gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga
     7921 caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg
     7981 aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt
     8041 tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt
     8101 acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga
     8161 ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac
     8221 gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct
     8281 ccttacgcat ctgtgcggta tttcacaccg caatggtgca ctctcagtac aatctgctct
     8341 gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg
     8401 cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat
     8461 ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt
     8521 catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt
     8581 cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga agcgttaatg
     8641 tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg gtcactgatg
     8701 cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa cgagagagga
     8761 tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt tgtgagggta
     8821 aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt caatgccagc
     8881 gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct gcgatgcaga
     8941 tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac gaaacacgga
     9001 aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc
     9061 acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc cgccagccta
     9121 gccgggtcct caacgacagg agcacgatca tgcgcacccg tggccaggac ccaacgctgc
     9181 ccgagatgcg ccgcgtgcgg ctgctggaga tggcggacgc gatggatatg ttctgccaag
     9241 ggttggtttg cgcattcaca gttctccgca agaattgatt ggctccaatt cttggagtgg
     9301 tgaatccgtt agcgaggtgc cgccggcttc cattcaggtc gaggtggccc ggctccatgc
     9361 accgcgacgc aacgcgggga ggcagacaag gtatagggcg gcgcctacaa tccatgccaa
     9421 cccgttccat gtgctcgccg aggcggcata aatcgccgtg acgatcagcg gtccaatgat
     9481 cgaagttagg ctggtaagag ccgcgagcga tccttgaagc tgtccctgat ggtcgtcatc
     9541 tacctgcctg gacagcatgg cctgcaacgc gggcatcccg atgccgccgg aagcgagaag
     9601 aatcataatg gggaaggcca tccagcctcg cgtcgcgaac gccagcaaga cgtagcccag
     9661 cgcgtcggcc gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt tggtggcggg
     9721 accagtgacg aaggcttgag cgagggcgtg caagattccg aataccgcaa gcgacaggcc
     9781 gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga gcgctgccgg
     9841 cacctgtcct acgagttgca tgataaagaa gacagtcata agtgcggcga cgatagtcat
     9901 gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgacg
     9961 ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga ggccgttgag
    10021 caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc ccccggccac
    10081 ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg
    10141 atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt
    10201 gatgccggcc acgatgcgtc cggcgtagag gatcgagatc tcgatcccgc gaaa
//
