LOCUS       sequence-443040-       12588 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12588)
  AUTHORS   Leterrier C, Vacher H, Fache MP, d'Ortoli SA, Castets F, 
            Autillo-Touati A, Dargent B
  TITLE     End-binding proteins EB3 and EB1 link microtubules to ankyrin G in 
            the axon initial segment.
  JOURNAL   Proc Natl Acad Sci U S A. 2011 May 24;108(21):8826-31. doi: 
            10.1073/pnas.1018671108. Epub 2011 May 6.
  PUBMED    21551097
REFERENCE   2  (bases 1 to 12588)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1018671108"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2011-05-24- 24"; volume: "108"; issue: "21"; pages: "8826-31"
COMMENT     Sequence Label: ankG-mCherry
FEATURES             Location/Qualifiers
     source          1..12588
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        132..435
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        436..639
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     590..610
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      8608..8617
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             8614..9324
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(8759..8777)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     9027..9046
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     polyA_signal    9447..9568
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9484..9503)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9538..9557
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(9575..10030)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9712..9733)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     9924..9943
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        10057..10161
                     /gene="bla"
                     /label=AmpR promoter
     promoter        10163..10520
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        10163..10354
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(10186..10206)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      10371..10506
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             10555..11349
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             10555..11349
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(10609..10628)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     11219..11238
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(11537..11556)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    11581..11628
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      11957..12545
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     12446..12465
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta
       61 ccgccatgca ttagttatta atagtaatca attacggggt cattagttca tagcccatat
      121 atggagttcc gcgttacata acttacggta aatggcccgc ctggctgacc gcccaacgac
      181 ccccgcccat tgacgtcaat aatgacgtat gttcccatag taacgccaat agggactttc
      241 cattgacgtc aatgggtgga gtatttacgg taaactgccc acttggcagt acatcaagtg
      301 tatcatatgc caagtacgcc ccctattgac gtcaatgacg gtaaatggcc cgcctggcat
      361 tatgcccagt acatgacctt atgggacttt cctacttggc agtacatcta cgtattagtc
      421 atcgctatta ccatggtgat gcggttttgg cagtacatca atgggcgtgg atagcggttt
      481 gactcacggg gatttccaag tctccacccc attgacgtca atgggagttt gttttggcac
      541 caaaatcaac gggactttcc aaaatgtcgt aacaactccg ccccattgac gcaaatgggc
      601 ggtaggcgtg tacggtggga ggtctatata agcagagctg gtttagtgaa ccgtcagatc
      661 cgctagcgct accggactca gatctcgagc tcaagcttcg aattcatggc tcatgccgcc
      721 tcccagttaa agaaaaacag ggatttagaa atcaatgcgg aagaagagac cgagaaaaaa
      781 aagaagcacc gtaaacggtc ccgggatcgc aagaaaaagt cggatgccaa tgcaagttac
      841 ttgagagcag ctcgggcggg gcacctggaa aaggcccttg actacatcaa aaatggagtg
      901 gacgtcaaca tctgtaacca gaatgggttg aatgcgctcc atcttgcttc caaagaaggc
      961 catgtggaag tggtctccga gctgctgcag agagaagcca acgtcgatgc agccacaaag
     1021 aaaggaaaca cagccttaca catcgcatcc ttggctggac aagcggaagt ggtcaaggtc
     1081 ttggttacga acggagcgaa cgtcaacgca cagtctcaga atggcttcac gccgctgtat
     1141 atggcggccc aggagaacca cctggaagtc gtcaggtttc ttctggacaa tggtgccagc
     1201 caaagcctgg ctacagagga tggcttcacg ccattggccg tggctcttca acaaggtcac
     1261 gaccaagtcg tatccctcct gctcgagaac gacacgaagg gaaaagtgcg cctcccagcc
     1321 ctccacatcg cggcccggaa agatgacacc aaagcggcag ctctgctcct acagaacgac
     1381 accaatgcag acatagagtc aaagatggtg gtgaatagag caactgagag tggcttcacc
     1441 tcactccaca tagctgccca ctacgggaat atcaatgtgg ccacgttgct gttaaaccga
     1501 gcggctgccg tggacttcac cgcacggaat gacatcactc ctttgcatgt tgcctcgaaa
     1561 cggggaaacg caaatatggt aaagctgttg ctcgaccggg gtgcgaagat cgacgccaag
     1621 accagggacg gtctgacccc gctgcactgc ggggcgagaa gtggccacga gcaggtggta
     1681 gagatgttgc tggacagagc cgcccccatc ctttcgaaaa ccaagaatgg actgtcccca
     1741 ctgcacatgg ccacacaagg agaccattta aactgcgtcc aactcctcct ccagcacaac
     1801 gtgcccgtgg atgacgtcac caacgactac ctgaccgccc tccacgtggc ggcccactgc
     1861 ggccattaca aagttgccaa ggttcttttg gataagaaag ctaaccccaa tgccaaagcc
     1921 ctgaatggct tcacccctct ccatatcgcc tgcaaaaaga accgaatccg agtaatggaa
     1981 ctccttttga agcacggtgc atctattcaa gccgtaaccg agtcgggcct taccccaatc
     2041 catgtcgctg ccttcatggg acatgtaaat atcgtgtcac agctaatgca tcatggagcc
     2101 tccccaaaca ccaccaacgt gagaggagaa acagcattgc atatggcggc tcggtcgggg
     2161 caagcagaag tggtgcggta tttggtacaa gatggagctc aggtagaagc aaaagctaag
     2221 gatgaccaga ccccactcca catttcagcc cgactcggga aggctgacat agtgcaacaa
     2281 ctgttacagc aaggagcgtc cccgaatgca gcaacaactt ccgggtacac cccccttcac
     2341 ctttcggcca gagaggggca tgaggatgta gctgccttcc ttctggacca tggagcatct
     2401 ttatccataa caacaaagaa gggattcacg cctctgcatg tggctgcaaa gtatgggaag
     2461 cttgaagtgg caagtctcct gctgcagaag agtgcgtctc ctgatgccgc tgggaagagc
     2521 gggctaactc cactgcatgt agcagcacat tacgataatc agaaagtggc ccttctgctt
     2581 ttggaccagg gagcctcacc ccacgcagct gcaaagaacg gttatacgcc actgcacatc
     2641 gccgccaaga agaaccagat ggacatagcc acgtccctgc tggagtacgg tgctgatgca
     2701 aacgccgtta cccggcaagg gattgcgtcc gtccaccttg cagcgcagga agggcacgtg
     2761 gacatggtgt cgctgctcct cagtagaaat gcgaacgtca acttgagcaa taagagcggc
     2821 ctcaccccac tccacctggc ggctcaagaa gaccgagtga acgtggctga ggtccttgtc
     2881 aaccaagggg cccacgtgga tgcacagaca aagatgggct acacgccgct ccatgtgggc
     2941 tgtcactatg gaaacatcaa aatagtcaat tttctgctgc agcattctgc gaaagttaat
     3001 gccaagacaa agaatggata cacgccactg caccaggcag cccagcaggg ccacacacac
     3061 atcatcaacg tcctgctcca gaacaacgct tcccccaatg aactcactgt gaatgggaac
     3121 acagccctgg ccatcgcccg acgccttggc tacatctcgg tggttgacac cctaaaggtc
     3181 gtgacagagg aaattatgac caccactacc atcacggaga agcacaaaat gaatgtccca
     3241 gaaacaatga atgaagtcct cgacatgtca gacgatgaag ttggtaaagc cagcgccccc
     3301 gaaaagctca gtgatgggga atatatctca gatggtgaag aaggtgaaga tgccatcaca
     3361 ggggacactg acaagtatct cgggccacag gacctcaagg agctgggcga tgactccctg
     3421 cctgccgagg gctatgtggg cttcagtctc ggagcccgtt ctgccagcct tcgctccttc
     3481 agttcggata ggtcctacac cctgaacaga agctcctatg cgcgggacag catgatgatc
     3541 gaggagcttc tggtgccatc caaagagcag caccttccat tcacgaggga gtttgactcc
     3601 gactccctca gacactacag ttgggcagca gacacactgg acaatgtgaa cctggtctcc
     3661 agccctgtgc attctgggtt tctggttagc tttatggtgg acgcgagagg gggctccatg
     3721 cgaggaagcc gccaccacgg gatgcggatc atcatccctc cgcgcaagtg tacggccccc
     3781 acccgcatca cttgccgcct ggtaaagaga cataaactgg ccaacccacc ccccatggtg
     3841 gaaggagagg gattagccag taggctggta gaaatgggtc ctgcgggggc acaattttta
     3901 ggcccggtca tagtggaaat ccctcatttt gggtccatga gaggaaagga gagggaactc
     3961 attgtcctcc ggagcgagaa cggagagacc tggaaggaac accagtttga cagcaaaaac
     4021 gaagacctct cggagctcct caatggcatg gacgaagaac ttgacagccc agaagagttg
     4081 ggcacaaagc gcatctgcag aatcatcacg aaggatttcc cccagtattt cgctgtggtt
     4141 tcccggatta agcaggaaag caaccagatt ggtcccgaag gtgggattct gagcagcacc
     4201 acggtccccc tcgtccaggc gtctttccca gagggtgcct taaccaaaag gatccgtgtg
     4261 ggtctgcagg ctcagcccgt accagaggag actgtgaaaa agatccttgg aaacaaagca
     4321 acgtttagcc caattgtcac ggtggagccg aggaggagga aattccataa gccgatcacc
     4381 atgaccatcc cggtgccccc accctcggga gaaggcgtgt ccaatgggta caagggggac
     4441 accacgccca gcctgcgact cctctgcagc attacaggag ggacctcgcc ggctcagtgg
     4501 gaagacatca caggcacaac cccgctgacc ttcataaagg actgtgtgtc tttcacaacc
     4561 aatgtttcag ccaggttctg gctggcggac tgccatcagg tgttagaaac cgtagggcta
     4621 gcctcgcagc tgtacagaga gttgatatgt gtcccctaca tggccaagtt tgttgttttt
     4681 gccaaaacaa acgacccagt ggaatcctcc ctaaggtgct tctgtatgac agacgacaga
     4741 gtggacaaaa ccctggagca gcaggagaat ttcgaggagg ttgccagaag caaagacatc
     4801 gaggttctgg aaggaaagcc catctatgtt gattgctatg gaaacctggc tcctctgacc
     4861 aaaggagggc agcagcttgt ttttaacttc tattctttca aagaaaacag actgccattt
     4921 tccatcaagg ttagagacac cagccaagag ccctgcggcc gtctgtcttt cctgaaggag
     4981 ccgaaaacaa caaaaggatt accccaaacg gctgtttgca acttaaatat tactctaccg
     5041 gcgcataaaa aggctgagaa ggcagacaga cgccagagct tcacatccct agccttacgt
     5101 aagcgctaca gctacttgac tgagcccagc atgaaaacag ttgaacggag ttcaggaaca
     5161 gcaagatccc ttcccactac ttattcacac aagccattct tttctacaag accataccag
     5221 tcctggacta cgactccact cacagtgccc gggccggcca aatcaggctc cttatccagc
     5281 tccccctcta acacgccgtc agcgtctccg ttaaaatcca tatggtctgt ttcaacgcct
     5341 tctccgatca aatccacgct aggcgcctca actacgtcat cagtcaaatc cattagtgat
     5401 gtggcgtctc ctattagatc tttccggaca atttcttcgc caataagaac ggtggcatcg
     5461 ccgtctccat acaataccca agttgcctct ggtacccttg ggagggttcc caccatcaca
     5521 gaggccacac ctataaaggg gctggctccc aactcaactt tgtcctctcg aacttctcct
     5581 gtgacgaccg cagggtctct gttggagagg tcgtccatca ccatgacacc ccctgcctcc
     5641 cccaaagcca acatcaccat gtattcctcc agcttgccat ttaagtccat tatcacgtca
     5701 gcggcaccgc tgatctcctc ccctttaaag tcagtggtgt ctccgaccaa gtctgcagct
     5761 gatgtcatct caacagctaa agctgcgatg gcatcgactc tctcctcccc cttaaagcag
     5821 atgtctggac atgcagaagt agcgctagtc aatgggtctg tttctcctct gaagtatcct
     5881 tcctcttccg ctttaattaa cggatgcaaa gccactgcca cattacagga caaaatttct
     5941 acacccacaa atgctgtgag ctcggtggtg agcgcggccc ctgacacggt ggagaaagcg
     6001 ctctccacca caacagccat gcccttttcc ccactcaggt cttacgtttc cgctgcagcg
     6061 ccctctgctt ttcagtccct tagagctccc tctgccagtg cactctacac ctccctcggg
     6121 ccttcagtag ctgttactac ctcatccgta acttcatcga taatcacagt gccagtatac
     6181 tcggtagtca atgttttgcc agaaccagca ttgaagaaac ttccagactc taactcgctc
     6241 acaaaatcgg cagcggcttt gctgtcgccc attaaaacat tgactacgga gacacgtcct
     6301 cagccccatt tcaatcgaac gtcatctccc gtcaagtcct ctctgttcct ggcatcctcg
     6361 gcccttaagc cgtcagtacc atcttcttta tcttccagtc aggagatctt aaaagatgtg
     6421 gccgagatga aggaggacct catgagaatg accgccatac tgcagacgga tgtgcctgag
     6481 gagaagccat tccagaccga cctcccccga gaagggagga tagacgacga agagcctttc
     6541 aaaatcgttg agaaagtgaa ggaagactta gtcaaagtca gtgagatact caaaaaggac
     6601 gtgtgtgtcg agagcaaagg gccacccaag tccccaaaga gtgacaaagg acactctcca
     6661 gaagatgact ggacggaatt tagttctgag gaaatacgag aagccaggca ggctgctgcc
     6721 agtcacgccc catccctgcc cgagagagta catggaaagg ccaacctcac cagagtcata
     6781 gactacctga ccaatgacat cgggagcagt tcactgacca acttaaagta caagtttgaa
     6841 gaggctaaga aggaaggaga ggagagacag aagagaattt taaagccagc catggccttg
     6901 caggagcata aactcaaaat gccgccagcc tccatgagac cttccacctc cgagaaggaa
     6961 ctgtgcaaga tggcggactc cttttttgga acagatgcca tcctagagtc tcccgatgac
     7021 ttttctcaac atgaccaaga caaaagtccc ttgtccgaca gtggctttga aacccgaagt
     7081 gagaaaacac cttcagcccc ccaaagtgcc gagagcaccg gccctaagcc actatttcat
     7141 gaagtcccca tcccccctgt catcacagag acgagaactg aagtagtcca cgtgatcagg
     7201 agctacgagc cctctactgg ggagatcccc cagtcccagc cagaggaccc cgtgtctccc
     7261 aaacctcccc ctacatttat ggagttggaa ccaaagccca ccgcccctag cattaaggaa
     7321 aaagtgaaag cattccaaat gaaagccagt agcgaggaag aggaccacag tcgggtttta
     7381 agcaaaggca tgcgtgtcaa agaagagact cacatcacca caaccacgag gatggtgtat
     7441 cactccccgc ctggcagtga gtgtgcctcc gagaggattg aggagaccat gtcagtccat
     7501 gacattatga aggcttttca gtccgggcga gacccttcca aagagctggc ggggctgttt
     7561 gaacacaagt cggccatgtc tccggatgtt gccaagtctg ctgctgagac ctcggcccag
     7621 cacgcagaga aggacaacca aatgaaaccc aaactggagc gtattataga ggtccacatc
     7681 gaaaaaggtc cacagagtcc ttgtgagcgg acggatatca ggatggcaat agtagccgat
     7741 cacctgggac ttagttggac agagctggca agggaactga atttttcagt ggatgaaatc
     7801 aaccaaatac gagtggaaaa tcccaattct ttaatttctc agagcttcat gttattaaaa
     7861 aagtgggtga cgagagacgg gaagaatgcc acaactgatg ccttaacttc ggtcttaaca
     7921 aaaattaacc gaatagacat agtaactctg ctggaaggac caatatttga ttatgggaat
     7981 atttcaggca ccagaagctt tgcagatgaa aacaatgttt tccatgaccc agttgatggt
     8041 tggcagaacg agacgccaag tggaagccta gagtccccgg cgcaagcgcg aagaataacc
     8101 ggtgggttac tggaccgtct ggacgacagc tctgaccagg ttcgggatcc cattacctca
     8161 tacctcacag gagaagctgg gaagttcgaa gcaaacggaa accacgcaga agtcattcca
     8221 gaagcaaagg ccaaggccta ctttcctgaa tcccaaaacg atatagggaa acagagcatc
     8281 aaggagaact tgaaaccaaa aacacacgga tgtggtcgcg ctgaggaacc agtgtcgccc
     8341 ctcacagcct accagaaatc tctagaagaa accagcaagc ttgtcataga agacgcacct
     8401 aaaccctgtg tgcctgtcgg catgaaaaag atgaccagga ctccggctga cggcaaagcc
     8461 aggctcaacc tccaggaaga ggaggggtcc gccaggtcag agcctaagca gggagaaggc
     8521 tataaggtga aaacaaagaa ggaaatccgg aatgtggaga agaaagccca ccagtcgacg
     8581 gtaccgcggg cccgggatcc accggtcgcc accatggtga gcaagggcga ggaggataac
     8641 atggccatca tcaaggagtt catgcgcttc aaggtgcaca tggagggctc cgtgaacggc
     8701 cacgagttcg agatcgaggg cgagggcgag ggccgcccct acgagggcac ccagaccgcc
     8761 aagctgaagg tgaccaaggg tggccccctg cccttcgcct gggacatcct gtcccctcag
     8821 ttcatgtacg gctccaaggc ctacgtgaag caccccgccg acatccccga ctacttgaag
     8881 ctgtccttcc ccgagggctt caagtgggag cgcgtgatga acttcgagga cggcggcgtg
     8941 gtgaccgtga cccaggactc ctccctgcag gacggcgagt tcatctacaa ggtgaagctg
     9001 cgcggcacca acttcccctc cgacggcccc gtaatgcaga agaagaccat gggctgggag
     9061 gcctcctccg agcggatgta ccccgaggac ggcgccctga agggcgagat caagcagagg
     9121 ctgaagctga aggacggcgg ccactacgac gctgaggtca agaccaccta caaggccaag
     9181 aagcccgtgc agctgcccgg cgcctacaac gtcaacatca agttggacat cacctcccac
     9241 aacgaggact acaccatcgt ggaacagtac gaacgcgccg agggccgcca ctccaccggc
     9301 ggcatggacg agctgtacaa gtagggcggc cgcgactcta gatcataatc agccatacca
     9361 catttgtaga ggttttactt gctttaaaaa acctcccaca cctccccctg aacctgaaac
     9421 ataaaatgaa tgcaattgtt gttgttaact tgtttattgc agcttataat ggttacaaat
     9481 aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg
     9541 gtttgtccaa actcatcaat gtatcttaag gcgtaaattg taagcgttaa tattttgtta
     9601 aaattcgcgt taaatttttg ttaaatcagc tcatttttta accaataggc cgaaatcggc
     9661 aaaatccctt ataaatcaaa agaatagacc gagatagggt tgagtgttgt tccagtttgg
     9721 aacaagagtc cactattaaa gaacgtggac tccaacgtca aagggcgaaa aaccgtctat
     9781 cagggcgatg gcccactacg tgaaccatca ccctaatcaa gttttttggg gtcgaggtgc
     9841 cgtaaagcac taaatcggaa ccctaaaggg agcccccgat ttagagcttg acggggaaag
     9901 ccggcgaacg tggcgagaaa ggaagggaag aaagcgaaag gagcgggcgc tagggcgctg
     9961 gcaagtgtag cggtcacgct gcgcgtaacc accacacccg ccgcgcttaa tgcgccgcta
    10021 cagggcgcgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt
    10081 ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa
    10141 taatattgaa aaaggaagag tcctgaggcg gaaagaacca gctgtggaat gtgtgtcagt
    10201 tagggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca
    10261 attagtcagc aaccaggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa
    10321 gcatgcatct caattagtca gcaaccatag tcccgcccct aactccgccc atcccgcccc
    10381 taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg
    10441 cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg
    10501 gaggcctagg cttttgcaaa gatcgatcaa gagacaggat gaggatcgtt tcgcatgatt
    10561 gaacaagatg gattgcacgc aggttctccg gccgcttggg tggagaggct attcggctat
    10621 gactgggcac aacagacaat cggctgctct gatgccgccg tgttccggct gtcagcgcag
    10681 gggcgcccgg ttctttttgt caagaccgac ctgtccggtg ccctgaatga actgcaagac
    10741 gaggcagcgc ggctatcgtg gctggccacg acgggcgttc cttgcgcagc tgtgctcgac
    10801 gttgtcactg aagcgggaag ggactggctg ctattgggcg aagtgccggg gcaggatctc
    10861 ctgtcatctc accttgctcc tgccgagaaa gtatccatca tggctgatgc aatgcggcgg
    10921 ctgcatacgc ttgatccggc tacctgccca ttcgaccacc aagcgaaaca tcgcatcgag
    10981 cgagcacgta ctcggatgga agccggtctt gtcgatcagg atgatctgga cgaagagcat
    11041 caggggctcg cgccagccga actgttcgcc aggctcaagg cgagcatgcc cgacggcgag
    11101 gatctcgtcg tgacccatgg cgatgcctgc ttgccgaata tcatggtgga aaatggccgc
    11161 ttttctggat tcatcgactg tggccggctg ggtgtggcgg accgctatca ggacatagcg
    11221 ttggctaccc gtgatattgc tgaagagctt ggcggcgaat gggctgaccg cttcctcgtg
    11281 ctttacggta tcgccgctcc cgattcgcag cgcatcgcct tctatcgcct tcttgacgag
    11341 ttcttctgag cgggactctg gggttcgaaa tgaccgacca agcgacgccc aacctgccat
    11401 cacgagattt cgattccacc gccgccttct atgaaaggtt gggcttcgga atcgttttcc
    11461 gggacgccgg ctggatgatc ctccagcgcg gggatctcat gctggagttc ttcgcccacc
    11521 ctagggggag gctaactgaa acacggaagg agacaatacc ggaaggaacc cgcgctatga
    11581 cggcaataaa aagacagaat aaaacgcacg gtgttgggtc gtttgttcat aaacgcgggg
    11641 ttcggtccca gggctggcac tctgtcgata ccccaccgag accccattgg ggccaatacg
    11701 cccgcgtttc ttccttttcc ccaccccacc ccccaagttc gggtgaaggc ccagggctcg
    11761 cagccaacgt cggggcggca ggccctgcca tagcctcagg ttactcatat atactttaga
    11821 ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc
    11881 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa
    11941 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
    12001 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
    12061 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt
    12121 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
    12181 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
    12241 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
    12301 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
    12361 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
    12421 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
    12481 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
    12541 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgct
//
