LOCUS       sequence-443091-        6377 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression vector for expression of hAT-7_PM transposase 
            (CMV promoter).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6377)
  AUTHORS   Zhang T, Tan S, Tang N, Li Y, Zhang C, Sun J, Guo Y, Gao H, Cai Y, 
            Sun W, Wang C, Fu L, Ma H, Wu Y, Hu X, Zhang X, Gee P, Yan W, Zhao 
            Y, Chen Q, Guo B, Wang H, Zhang YE
  TITLE     Heterologous survey of 130 DNA transposons in human cells highlights
            their functional divergence and expands the genome engineering 
            toolbox.
  JOURNAL   Cell. 2024 Jul 11;187(14):3741-3760.e30. doi: 
            10.1016/j.cell.2024.05.007. Epub 2024 Jun 5.
  PUBMED    38843831
REFERENCE   2  (bases 1 to 6377)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2024.05.007"; journalName: "Cell"; date: 
            "2024-07-11- 11"; volume: "187"; issue: "14"; pages: "3741-3760.e30"
COMMENT     Sequence Label: pCMV-hAT-7_PM
FEATURES             Location/Qualifiers
     source          1..6377
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     164..183
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        258..362
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(363..431,432..1223)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(581..600)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1394..1982
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1883..1902
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2136..2153
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2270..2291
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2306..2311,2312..2329,2330..2336)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2344..2360
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2349..2371
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2368..2384
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2368..2384
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     polyA_signal    2433..2554
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2444..2463)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     2498..2517
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        complement(2720..3049)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      2734..2869
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(2788..2807)
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     3034..3054
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      complement(3063..3491)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3173..3194)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3385..3404
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     polyA_signal    3537..3761
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     3750..3767
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     promoter        3787..3805
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     3787..3804
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(5722..5741)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5723..5741)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(5786..5989)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(5815..5835)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        5990..6369
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 tatctggccc gtacatcgcg aagcagcgca aaacgcctaa ccctaagcag attcttcatg
       61 caattgtcgg tcaagccttg ccttgttgta gcttaaattt tgctcgcgca ctactcagcg
      121 acctccaaca cacaagcagg gagcagatac tggcttaact atgcggcatc agagcagatt
      181 gtactgagag tgcaccatag gggatcggga gatctcccga tccgtcgacg tcaggtggca
      241 cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata
      301 tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga
      361 gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc
      421 ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg
      481 cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc
      541 ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat
      601 cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact
      661 tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat
      721 tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga
      781 tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc
      841 ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga
      901 tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag
      961 cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc
     1021 gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt
     1081 ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct
     1141 acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg
     1201 cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg
     1261 atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca
     1321 tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga
     1381 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
     1441 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
     1501 aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt
     1561 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
     1621 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
     1681 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
     1741 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
     1801 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
     1861 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
     1921 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
     1981 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
     2041 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
     2101 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
     2161 aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct
     2221 ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt
     2281 agctcactca ttaggcaccc caggctttac actttatgct tccggctcgt atgttgtgtg
     2341 gaattgtgag cggataacaa tttcacacag gaaacagcta tgaccatgat tacgccaagc
     2401 tctagctaga ggtcgacggt atacagacat gataagatac attgatgagt ttggacaaac
     2461 cacaactaga atgcagtgaa aaaaatgctt tatttgtgaa atttgtgatg ctattgcttt
     2521 atttgtaacc attataagct gcaataaaca agttggggtg ggcgaagaac tccagcatga
     2581 gatccccgcg ctggaggatc atccagccgg cgtcccggaa aacgattccg aagcccaacc
     2641 tttcatagaa ggcggcggtg gaatcgaaat ctcgtgatgg caggttgggc gtcgcttggt
     2701 cggtcatttc ggggagcttt ttgcaaaagc ctaggcctcc aaaaaagcct cctcactact
     2761 tctggaatag ctcagaggca gaggcggcct cggcctctgc ataaataaaa aaaattagtc
     2821 agccatgggg cggagaatgg gcggaactgg gcggagttag gggcgggatg ggcggagtta
     2881 ggggcgggac tatggttgct gactaattga gatgcatgct ttgcatactt ctgcctgctg
     2941 gggagcctgg ggactttcca cacctggttg ctgactaatt gagatgcatg ctttgcatac
     3001 ttctgcctgc tggggagcct ggggactttc cacaccctaa ctgacacaca ttccacagaa
     3061 ttaattcgcg ttaaattttt gttaaatcag ctcatttttt aaccaatagg ccgaaatcgg
     3121 caaaatccct tataaatcaa aagaatagac cgagataggg ttgagtgttg ttccagtttg
     3181 gaacaagagt ccactattaa agaacgtgga ctccaacgtc aaagggcgaa aaaccgtcta
     3241 tcagggcgat ggcccactac gtgaaccatc accctaatca agttttttgg ggtcgaggtg
     3301 ccgtaaagca ctaaatcgga accctaaagg gagcccccga tttagagctt gacggggaaa
     3361 gccggcgaac gtggcgagaa aggaagggaa gaaagcgaaa ggagcgggcg ctagggcgct
     3421 ggcaagtgta gcggtcacgc tgcgcgtaac caccacaccc gccgcgctta atgcgccgct
     3481 acagggcgcg tggggatacc ccctagagcc ccagctggtt ctttccgcct cagaagccat
     3541 agagcccacc gcatccccag catgcctgct attgtcttcc caatcctccc ccttgctgtc
     3601 ctgccccacc ccacccccca gaatagaatg acacctactc agacaatgcg atgcaatttc
     3661 ctcattttat taggaaagga cagtgggagt ggcaccttcc agggtcaagg aaggcacggg
     3721 ggaggggcaa acaacagatg gctggcaact agaaggcaca gtcgaggctg atcagcgagc
     3781 tctagcattt aggtgacact atagaatagg gccctctaga tgcatgctcg agcggccgct
     3841 cattaatggc tgcaatgctg ctggtcaccc attttggcca gcgcatccag atcaggggtg
     3901 attttggtgg tcgcaatgcg taagatgctg cgcagatgct gatcggtcag tttgctgcga
     3961 tggcgcgctt tgttggcgtt catcacgctg aaggtctgct cgcacacata ggtgctgccg
     4021 aacagggtca gcattttttg ggcggtacgg cgcagattag gaaaggtcgc acggttcagg
     4081 ctcgcataaa agtcgttcag cttcacgctg ttgaatttct ctttcagcac gctatcgctc
     4141 tgcagatcaa tcagttccag ctgtaattcc tgaggcgcgg tttcgctatc ctggcttaac
     4201 ggacagctca ctaactgcag gctctgttcg atgttttcaa aatcgctaaa gcggcggcaa
     4261 aattcgccat gcagatcttc caggcttttg ctatactttt tcgcgttgcg cggtgcttct
     4321 tcttgcatcg ccagggtagg gaaatgggcg aagcttttgt tggcaatctg gcggctaaac
     4381 agggtcagtt tgcttttaaa ggccttcacg tgcttataca tgtcgtgcac aaactgatct
     4441 ttgccctgca gtttcacgtt cagttcgttc atatggctga agatgtccac cgcgaatgcg
     4501 aaatcgctca gccagttttt atcgctcagc tcaggaaact catcgctttt gcccatcagc
     4561 tccagaaacg caatgatatc ttccttcagt tcccacacgc gctgcagaac tttacccagg
     4621 cttaaccagc gaacgcggct atgatacagc agatcctgat gatcggcgtc ggtttcttcc
     4681 aggaaggtga taaactggcg atgttgtaaa ccgcgggcgc gaataaagtt caccagtttc
     4741 accgcagggt tcacaacgtg gttcagctgc agcacgcttt tgcacaggct ttcctggtgg
     4801 atgatgcaat gcaggaaaat cagatcctga tccgggttct cctctttcac tttgttctga
     4861 atgcgtttca gcaggcccac gtttttgccg gtcaggttag ggctaccatc ggtggtcacg
     4921 ttaaccagct tgctccacgg cagtttcatg cgttcaatca cggcgctcac ctggttatac
     4981 aggtcttcgc cgcgagtggt acctttcagg ctttccatgg tcagaaattc ctcggtaatg
     5041 gcaaagctgt catcaatgcc gcgaataaaa atcagcagct gcgcggtatc cttcacatcg
     5101 gtgctttcat ccagcgccag gctgtacagc tcaaagctat cgcttttttt gttcagctgg
     5161 ctcgcaatgt tctcatcaat cagttccaca cgacgggtaa cggtacgacg gctcaggctc
     5221 actttctcga atttgctttt gatttccggg cacagaatat ccgcggtctg caccatgcat
     5281 tccttcacaa attcgccttc gctgaacggt ttgctcgctt tggctatctc gaatgccacc
     5341 aggaagctgg ctttggtggt gctctcttga atcgcggtct ggcggtggaa aaaatgctgc
     5401 tgggtctgta agttggccgc taaacgttgc gcggttgctg cacgttcttg ggtgctctgt
     5461 ttgctcgcgt agttggcatg cttagtcgcg aagtggcggc taatgttgta ctccttgaac
     5521 accgccacgg tttcacggca gatcagacac accgctttgc tctgcacctc ggtaaagaaa
     5581 tacttggtgg tccattcggt gttgaacacg cggcactcgt tatccacctt gcgtttacgg
     5641 ctaccgctca tggtggcggc gaattccagc acactggcgg ccgttactag tggatccgag
     5701 ctcggtacca agcttgggtc tccctatagt gagtcgtatt aatttcgata agccagtaag
     5761 cagtgggttc tctagttagc cagagagctc tgcttatata gacctcccac cgtacacgcc
     5821 taccgcccat ttgcgtcaat ggggcggagt tgttacgaca ttttggaaag tcccgttgat
     5881 tttggtgcca aaacaaactc ccattgacgt caatggggtg gagacttgga aatccccgtg
     5941 agtcaaaccg ctatccacgc ccattgatgt actgccaaaa ccgcatcacc atggtaatag
     6001 cgatgactaa tacgtagatg tactgccaag taggaaagtc ccataaggtc atgtactggg
     6061 cataatgcca ggcgggccat ttaccgtcat tgacgtcaat agggggcgta cttggcatat
     6121 gatacacttg atgtactgcc aagtgggcag tttaccgtaa atactccacc cattgacgtc
     6181 aatggaaagt ccctattggc gttactatgg gaacatacgt cattattgac gtcaatgggc
     6241 gggggtcgtt gggcggtcag ccaggcgggc catttaccgt aagttatgta acgcggaact
     6301 ccatatatgg gctatgaact aatgaccccg taattgatta ctattaataa ctagtcaata
     6361 atcaatgtca acgcgta
//
