LOCUS       sequence-44312-e         780 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 780)
  AUTHORS   Gabut M, Samavarchi-Tehrani P, Wang X, Slobodeniuc V, O'Hanlon D, 
            Sung HK, Alvarez M, Talukder S, Pan Q, Mazzoni EO, Nedelec S, 
            Wichterle H, Woltjen K, Hughes TR, Zandstra PW, Nagy A, Wrana JL, 
            Blencowe BJ
  TITLE     An alternative splicing switch regulates embryonic stem cell 
            pluripotency and reprogramming.
  JOURNAL   Cell. 2011 Sep 30;147(1):132-46. Epub 2011 Sep 15.
  PUBMED    21924763
REFERENCE   2  (bases 1 to 780)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell."; 
            date: "2011-09-30"; volume: "147(1)"; pages: "132-46. Epub 2011 Sep 
            15"
COMMENT     Sequence Label: pLKO-tet-puro-sh-control
FEATURES             Location/Qualifiers
     source          1..780
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     intron          36..608
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     primer_bind     616..635
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     primer_bind     663..682
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        663..681
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 gaccgaatca ccgacctctc tccccagggg gatctgtgag tttggggacc cttgattgtt
       61 ctttcttttt cgctattgta aaattcatgt tatatggagg gggcaaagtt ttcagggtgt
      121 tgtttagaat gggaagatgt cccttgtatc accatggacc ctcatgataa ttttgtttct
      181 ttcactttct actctgttga caaccattgt ctcctcttat tttcttttca ttttctgtaa
      241 ctttttcgtt aaactttagc ttgcatttgt aacgaatttt taaattcact tttgtttatt
      301 tgtcagattg taagtacttt ctctaatcac ttttttttca aggcaatcag ggtatattat
      361 attgtacttc agcacagttt tagagaacaa ttgttataat taaatgataa ggtagaatat
      421 ttctgcatat aaattctggc tggcgtggaa atattcttat tggtagaaac aactacatcc
      481 tggtcatcat cctgcctttc tctttatggt tacaatgata tacactgttt gagatgagga
      541 taaaatactc tgagtccaaa ccgggcccct ctgctaacca tgttcatgcc ttcttctttt
      601 tcctacagct cctgggcaac gtgctggtta ttgtgctgtc tcatcatttt ggcaaagaat
      661 tgtaatacga ctcactatag ggcgaattga tatgtctaga ttagataaaa gtaaagtgat
      721 taacagcgca ttagagctgc ttaatgaggt cggaatcgaa ggtttaacaa cccgtaaact
//
