LOCUS       sequence-443123-        4664 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway-compatible Entry vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4664)
  AUTHORS   Weller B, Lin CW, Pogoutse O, Sauer M, Marin-de la Rosa N, Strobel 
            A, Young V, Knapp JJ, Rayhan A, Falter C, Kim DK, Roth FP, 
            Falter-Braun P
  TITLE     A resource of human coronavirus protein-coding sequences in a 
            flexible, multipurpose Gateway Entry clone collection.
  JOURNAL   G3 (Bethesda). 2023 Jul 5;13(7):jkad105. doi: 
            10.1093/g3journal/jkad105.
  PUBMED    37267226
REFERENCE   2  (bases 1 to 4664)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "G3 
            (Bethesda)."; date: "2023-07-5"; volume: "13(7):jkad105. doi"; 
            pages: " 10.1093/g3journal/jkad105"
COMMENT     Sequence Label: pEntry_HCoV-OC43-nsp2
FEATURES             Location/Qualifiers
     source          1..4664
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..100
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1975..2074)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2091..2110)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2092..2110)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2115..2131)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2115..2131)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             2562..3353
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      3446..4034
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3935..3954
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4188..4205
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(4364..4391)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(4483..4569)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     4618..4640
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     4632..4649
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     4633..4649
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacaaat tgatgagcaa
       61 tgctttttta taatgccaac tttgtacaaa aaagcagaag ggccgtcaag gccaggatcc
      121 atgggatccg ttaaaccact tctctatgta gaccagtatg gttgtgatta tactggtagt
      181 cttgcagatg gcttagaggc ttatgctgat aagacattgc aagaaatgaa ggcattattt
      241 cctacttgga gtcaggaact cctttttgat gtaattgtgg catggcatgt tgtgcgtgat
      301 ccacgttatg ttatgagatt gcagagtgct gctactatac gtagtgttgc atatgttgct
      361 aatcctactg aagacttgtg tgatggttct gttgttataa aagaacctgt gcatgtttat
      421 gcagatgact ctattatttt acgtcaatat aatttagttg acattatgag tcatttttat
      481 atggaggcag atacagttgt aaatgctttt tatggtgttg ctttgaaaga ttgcggtttt
      541 gttatgcagt ttggttacat tgattgcgaa caagactcgt gtgattttaa aggttggatt
      601 cctggtaaca tgatagatgg ttttgcttgc accacttgtg gtcatgttta tgaagtaggt
      661 gatttgatgg cacaatcttc aggtgttttg cctgttaacc ctgtattgca tactaagagt
      721 gcagcaggct atggtggttt tggttgtaaa gattctttta ctctgtatgg ccaaactgta
      781 gtttattttg gaggttgtgt gtattggagt ccagcacgta atatatggat tcctatatta
      841 aaatcctctg ttaagtcata tgacagtttg gtttatactg gagttttagg ttgcaaggct
      901 attgtaaagg aaacaaatct catttgcaaa gctttgtacc ttgattatgt tcaacacaag
      961 tgtggcaatt tacaccaacg ggagttgcta ggtgtttcag atgtgtggca taaacaattg
     1021 ctattaaata gaggtgttta taaacctctg ttagagaata ttgattattt taatatgcgg
     1081 cgcgctaaat ttagtttaga aacttttact gtttgtgcag atggctttat gccttttctt
     1141 ttagatgatt tagttccacg cgcatattat ttggcagtaa gtggtcaagc attttgtgat
     1201 tatgcagata aactttgcca tgccgttgtg tctaagagta aagagttact tgatgtgtct
     1261 ctggattctt taggtgcagc tatacattat ttgaattcta agattgttga tttggctcaa
     1321 cattttagtg attttggaac aagtttcgtt tctaaaattg ttcatttctt taagactttt
     1381 actactagca ctgctcttgc atttgcatgg gttttatttc atgttttgca tggtgcttat
     1441 atagtagtgg agagtgatat atattttgtt aaaaacattc ctcgttatgc tagtgctgtt
     1501 gcacaagcat ttcagagtgt tgctaaagtt gtactggact ctttaagagt tacttttatt
     1561 gatggccttt cttgttttaa gattggacgt agaagaattt gtctttcagg cagaaaaatt
     1621 tatgaagttg agcgtggctt gttacattca tcccaattgc cattagatgt ttatgattta
     1681 accatgccta gtcaagttca gaaagccaag caaaaaccta tttatttaaa aggttctggt
     1741 tctgattttt cattagcgga tagtgtagtt gaagttgtta caacttcact tacaccatgt
     1801 ggttattctg aaccacctaa agttgcagct aaaatttgca ttgtggataa tgtttatatg
     1861 gccaaggctg gtgacaaata ttaccctgtt gtggttgatg atcatgttgg actcttggat
     1921 caagcatgga gagttccttg tgctcttaat taagacgcga tcgcggcctc atgggcccag
     1981 ctttcttgta caaagttggc attataaaaa ataattgctc atcaatttgt tgcaacgaac
     2041 aggtcactat cagtcaaaat aaaatcatta tttgccatcc agctgatatc ccctatagtg
     2101 agtcgtatta catggtcata gctgtttcct ggcagctctg gcccgtgtct caaaatctct
     2161 gatgttacat tgcacaagat aaaaatatat catcatgcct cctctagacc agccaggaca
     2221 gaaatgcctc gacttcgctg ctacccaagg ttgccgggtg acgcacaccg tggaaacgga
     2281 tgaaggcacg aacccagtgg acataagcct gttcggttcg taagctgtaa tgcaagtagc
     2341 gtatgcgctc acgcaactgg tccagaacct tgaccgaacg cagcggtggt aacggcgcag
     2401 tggcggtttt catggcttgt tatgactgtt tttttggggt acagtctatg cctcgggcat
     2461 ccaagcagca agcgcgttac gccgtgggtc gatgtttgat gttatggagc agcaacgatg
     2521 ttacgcagca gggcagtcgc cctaaaacaa agttaaacat tatgagggaa gcggtgatcg
     2581 ccgaagtatc gactcaacta tcagaggtag ttggcgtcat cgagcgccat ctcgaaccga
     2641 cgttgctggc cgtacatttg tacggctccg cagtggatgg cggcctgaag ccacacagtg
     2701 atattgattt gctggttacg gtgaccgtaa ggcttgatga aacaacgcgg cgagctttga
     2761 tcaacgacct tttggaaact tcggcttccc ctggagagag cgagattctc cgcgctgtag
     2821 aagtcaccat tgttgtgcac gacgacatca ttccgtggcg ttatccagct aagcgcgaac
     2881 tgcaatttgg agaatggcag cgcaatgaca ttcttgcagg tatcttcgag ccagccacga
     2941 tcgacattga tctggctatc ttgctgacaa aagcaagaga acatagcgtt gccttggtag
     3001 gtccagcggc ggaggaactc tttgatccgg ttcctgaaca ggatctattt gaggcgctaa
     3061 atgaaacctt aacgctatgg aactcgccgc ccgactgggc tggcgatgag cgaaatgtag
     3121 tgcttacgtt gtcccgcatt tggtacagcg cagtaaccgg caaaatcgcg ccgaaggatg
     3181 tcgctgccga ctgggcaatg gagcgcctgc cggcccagta tcagcccgtc atacttgaag
     3241 ctagacaggc ttatcttgga caagaagaag atcgcttggc ctcgcgcgca gatcagttgg
     3301 aagaatttgt ccactacgtg aaaggcgaga tcaccaaggt agtcggcaaa taaccctcga
     3361 gccacccatg accaaaatcc cttaacgtga gttacgcgtc gttccactga gcgtcagacc
     3421 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct
     3481 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa
     3541 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag
     3601 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc
     3661 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg
     3721 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca
     3781 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat
     3841 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg
     3901 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc
     3961 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc
     4021 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc
     4081 cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg
     4141 cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga
     4201 gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc
     4261 attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa
     4321 ttaatacgcg taccgctagc caggaagagt ttgtagaaac gcaaaaaggc catccgtcag
     4381 gatggccttc tgcttagttt gatgcctggc agtttatggc gggcgtcctg cccgccaccc
     4441 tccgggccgt tgcttcacaa cgttcaaatc cgctcccggc ggatttgtcc tactcaggag
     4501 agcgttcacc gacaaacaac agataaaacg aaaggcccag tcttccgact gagcctttcg
     4561 ttttatttga tgcctggcag ttccctactc tcgcgttaac gctagcatgg atgttttccc
     4621 agtcacgacg ttgtaaaacg acggccagtc ttaagctcgg gccc
//
