LOCUS       sequence-443136-       10385 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MARF1 expression in Schneider cells for imaging.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10385)
  AUTHORS   Salgania HK, Metz J, Jeske M
  TITLE     ReLo is a simple and rapid colocalization assay to identify and 
            characterize direct protein-protein interactions.
  JOURNAL   Nat Commun. 2024 Apr 3;15(1):2875. doi: 10.1038/s41467-024-47233-4.
  PUBMED    38570497
REFERENCE   2  (bases 1 to 10385)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2024-04-3"; volume: "15(1):2875. doi"; pages: " 
            10.1038/s41467-024-47233-4"
COMMENT     Sequence Label: pAc5.1-PH-mCherry-MARF1 isoform D (HK56)
FEATURES             Location/Qualifiers
     source          1..10385
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        56..2565
                     /label=Ac5 promoter
                     /note="Drosophila melanogaster actin 5C promoter"
     CDS             2984..3691
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(3129..3147)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     3397..3416
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             7691..7732
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             7742..7759
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(7782..7799)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7832..7966
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7856..7875)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     7910..7929
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      complement(8567..9155)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8647..8666)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9326..10117,10118..10186))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9949..9968
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10187..10291)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     10359..10377
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 cttcaagaat taattctata ttctaaaaac acaaatgata cttctaaaaa aaaatcatga
       61 atggcatcaa ctctgaatca aatctttgca gatgcaccta cttctcattt ccactgtcac
      121 atcatttttc cagatctcgc tgcctgttat gtggcccaca aaccaagaca cgttttatgg
      181 ccattaaagc tggctgatcg tcgccaaaca ccaaatacat aatgaatatg tacacattcg
      241 agaaagaagc gatcaaagaa gcgtcttcgg gcggagtagg agaatgcgga ggagaaggag
      301 aacgagctga tctagtatct ctccacaatc caatgccaac tgaccaactg gccatattcg
      361 gagcaatttg aagccaattt ccatcgcctg gcgatcgctc cattcttggc tatatgtttt
      421 tcaccgttac ccggggccat tttcaaagac tcgtcggcaa gataagattg tgtcactcgc
      481 tgtctctctt catttgtcga agaatgctga ggaatttcgc gatgacgtcg gcgagtattt
      541 tgaagaatga gaataatttg tatttatacg aaaatcagtt agtggaattt tctacaaaaa
      601 catgttatct atagataatt ttgttgcaaa atatgttgac tatgacaaag attgtatgta
      661 tataccttta atgtattctc attttcttat gtatttataa tggcaatgat gatactgatg
      721 atattttaag atgatgccag accaaaaggc ttgaatttct gcgtcttttg ccgaacgcag
      781 tgcatgtgca attgttgttt tttggaatat tcaattttcg gactgtccgc tttgatttca
      841 gtttcttggc ttattcaaaa agcaaagtaa agccaaaaaa gcgagatggc aataccaaat
      901 gcggcaaaac ggtagtggaa ggaaaggggt gcggggcagc ggaaggaagg gtggggcggg
      961 gcgtggcggg gtctgtggct gggcgcgacg tcaccgacgt tggagccact cctttgacca
     1021 tgtgtgcgtg tgtgtattat tcgtgtctcg ccactcgccg gttgtttttt tctttttatg
     1081 ctgcgctctc tctagcgcca tctcgcttac gcatgctcaa cgcaccgcat gttgccgttt
     1141 ccttttatgc gtcattttgg ctcgaaatag gcaattattt aaacaaagat tagtcaacga
     1201 aaacgctaaa ataaataagt ctacaatatg gttacttatt gccatgtgtg tgcagccaac
     1261 gatagcaaca aaagcaacaa cacaggtggc tttccctctt tcactttttg tttgcaagcc
     1321 gcgtgcgagc aagacggcac gaccggcaaa cgcaattacg ctgacaaaga gcagacgaag
     1381 ttttggcgaa aaacatcaag gcgcctgata cgaatgcatt tgcaataaca attgcgatat
     1441 ttaatattgt ttatgaagct gtttgacttc aaaacacaca aaaaaaaaaa taaaacaaat
     1501 tatttgaaag agaattagga atcggacgct tatcgttagg gtaacaacaa gaaatgctta
     1561 ctgagtcaca gcctctggaa aactgccgca agccagagag agagagaaaa agagggagag
     1621 cagcttagac cgcatgtgct tgtgtgtgag gcgtctctct cttcgtctct gttgcgcaaa
     1681 cgcatagact gcactgaaaa aatcgattac ctatttttta tgaatgaata tttgcactat
     1741 tactattcaa aactattaag atagcaatca cattcaatag ccaaatacta taccacctga
     1801 gcgatgcaac gaaatgatca atttgagcaa aaatgctgca tatttaggac ggcatcatta
     1861 tagaaatgct tcttgctgtg tacttttctc tcgtctggca gctgtttcgc cgttattgtt
     1921 aaaaccggct taagttaggt gtgttttcta cgactagtga atgccctact agaagatgtg
     1981 tgttgcacaa aatgtccctg gaataaccaa tttgaagtgc agatagcagt aaacgtaagc
     2041 taatatgaat attatttaac tgtaatgttt taatatcgct ggacattact aataaaccca
     2101 ctataaacac atgtacatat gtatgttttg gcatacaatg agtagttggg gaaaaaatgt
     2161 gtaaaagcac cgtgaccatc acagcataaa gataaccagc tgaagtatcg aatatgagta
     2221 acccccaaat tgaatcacat gccgcaactg ataggaccca tggaagtaca ctcttcatgg
     2281 cgatatacaa gacacacaca agcacgaaca cccagttgcg gaggaaattc tccgtaaatg
     2341 aaaacccaat cggcgaacaa ttcataccca tatatggtaa aagttttgaa cgcgacttga
     2401 gagcggagag cattgcggct gataaggttt tagcgctaag cgggctttat aaaacgggct
     2461 gcgggaccag ttttcatatc actaccgttt gagttcttgt gctgtgtgga tactcctccc
     2521 gacacaaagc cgctccatca gccagcagtc gtctaatcca gagaccccgg atcggggtac
     2581 caacatgcac ggtctccagg atgatccaga tctgcaggct ctgctgaaag gttctcagct
     2641 cctcaaagtc aaaagcagct cctggcgtcg tgaacgcttc tacaaactgc aggaggactg
     2701 caaaaccatc tggcaggaat cccgtaaagt aatgcgctcc ccagagtctc agctcttcag
     2761 cattgaggac attcaggagg tacgtatggg tcaccgtacc gaaggtctgg agaaattcgc
     2821 tcgcgatatc ccagaagatc gctgcttctc tatcgtcttc aaagaccagc gcaacaccct
     2881 ggatctgatt gctccatctc cagcagatgc tcagcactgg gttcagggtc tccgcaaaat
     2941 cattcaccac tccggttcta tggaccagcg tcagaaaggt accatggtga gcaagggcga
     3001 ggaggataac atggccatca tcaaggagtt catgcgcttc aaggtgcaca tggagggctc
     3061 cgtgaacggc cacgagttcg agatcgaggg cgagggcgag ggccgcccct acgagggcac
     3121 ccagaccgcc aagctgaagg tgaccaaggg tggccccctg cccttcgcct gggacatcct
     3181 gtcccctcag ttcatgtacg gctccaaggc ctacgtgaag caccccgccg acatccccga
     3241 ctacttgaag ctgtccttcc ccgagggctt caagtgggag cgcgtgatga acttcgagga
     3301 cggcggcgtg gtgaccgtga cccaggactc ctccctgcag gacggcgagt tcatctacaa
     3361 ggtgaagctg cgcggcacca acttcccctc cgacggcccc gtaatgcaga agaagaccat
     3421 gggctgggag gcctcctccg agcggatgta ccccgaggac ggcgccctga agggcgagat
     3481 caagcagagg ctgaagctga aggacggcgg ccactacgac gctgaggtca agaccaccta
     3541 caaggccaag aagcccgtgc agctgcccgg cgcctacaac gtcaacatca agttggacat
     3601 cacctcccac aacgaggact acaccatcgt ggaacagtac gaacgcgccg agggccgcca
     3661 ctccaccggc ggcatggacg agctgtacaa gccaagcttg aattctgcaa cgtgcatgtt
     3721 tcagcgacac acgtcggtga tcgatcttca tcgttggaat tatcctgaat gctggccgtt
     3781 tgatagaaac ggaaaagtcg aaaacatata tgtaatggaa cattccgatc ccgtgcctta
     3841 tcaacagcta tcgacattat cgccgaccac cgcactatac tcgaacttta acaactcttt
     3901 ggaagcagga accgcagaaa gactacgcct taataaaact gggtcacagg tccacagcct
     3961 atatgtcggc gacggtcaag tgcaaaatgc ttctgaaccc ttatcttttc ccagccacct
     4021 taatctgtac caatatgttc gtaccccggc aaacttcact attaacagcc gcaaccgata
     4081 tattccgtac tataacaatc gttcctactc tgcttcaaca actggtttcc ctatagccag
     4141 tactccgctg atcagcagcc atgcgcacat ccaagaccaa caaggtgcag cgtcttatca
     4201 aacgattgtt ccgctttatc agaccgcaag ccactgtgcg cagcaacata caacattggc
     4261 atcggtagct ccaatcgtgg acaattcatt cgttgaaagc accacttgcc ccatggccgg
     4321 catcacctct cacaacatca aaatgaaaat gattcctccc aatgtattgt tgccttgctc
     4381 caggcctacg acaaccgcgg tgaccatcca aaggtcaacc ggagaagacg gtggagataa
     4441 agaaggtcat acttttgcta agattacgga gatgagcgat gctgttactt tgcagatcac
     4501 caacttggac tactcgctgg acgaatcaca tatacgcagc tttctactta atcagcttaa
     4561 gcccattacg ccagttgtgt cacttgtctt cgagggaagc tcctatgcca aagtcactgt
     4621 tccagatctt tatttcgcaa agcaggtcgt ctccaatttg caccgcaaga agattggaca
     4681 caaacgcatg ctggtcagtt atactcgaga ttcctccctc accgaggtca acacactccg
     4741 ctgccaggtc gcaggacttc taaaggatgt cccattcaac accctcccca tgtataagtt
     4801 tagagaacta tttcagtccc gcttcaaaac ctctatcagt gttttagatt tgtacaaaat
     4861 gcaagacata tgtaccatta actcggataa caatgaagaa aagtttatca gcttaaatcc
     4921 ggaattagta aacaccctcg atatatcccc cttaatggaa ggactccaac atagtgttcc
     4981 ttactgtagt attcatttta aaaaggagca gcacaaggga tgggccgagc aggaaatcga
     5041 gccactacca aacgtcttca tgagcatttc ggaaattcag aaactaattt atccacttct
     5101 caaggtacac acgggggata ttccggtggc tacccttctt cactgcgtta aagaagagct
     5161 taatgtgtca ataatggcga acgaaaatgg cgttaacttg gagcatttga tctgctgtgt
     5221 tcagggaatt caagttcggg caaacaattt tggaattaaa attctcggat ggttagaaat
     5281 aaacaaagaa atgcaatcag gaacttttaa tgccagttct aatacatgta gcctgactgc
     5341 aagtgatcgc acaaattgtg gaagctattt caaaaactca gttgccgatc cgcttttcca
     5401 aatttcccgg gaggttatag agctgctaaa gatgtcacca aaatcaacta tgaaattcaa
     5461 ccgttttatt ccggcctacc acaatcactt tggaaagcaa tgtcgagttg cagactatgg
     5521 ctatacaaag ttaatcgaat tgtttgaggc attatcaaac gttgtccaaa ttatgggaga
     5581 cggcgaaaat cgtcaaatta cgctatccca ccgtattcaa atacgcaggt ttacatcaga
     5641 cttattgcgt gtattgcgcg ctaatggtaa caactcagtc ctgctttccc agttgccatt
     5701 agtttttaca cagacccaaa ataaaacttt tgatattact gattatggag tgtgcgacct
     5761 tatagacatt ctagacggct tggtaagctc caacatcgtg actttaggcg cagcacagaa
     5821 cggaaaagat attcttatat caatgccaaa acgaaagcag acaaacagtg aattggagaa
     5881 aacgtgtgtg ttcgccggcg aaatggttga gctcttccaa aacgctcttc agtacactat
     5941 tctatttcag aagtttgtac ggtcctatca ttaccacttt gcataccaat gtcgtctcag
     6001 tgattacggc tttcttaaat tggccgatct tctagatgca attaatggtt tggtggaaat
     6061 gaagttgaca agtgatgagg acaagaaaat agttctctcc ccacaagtag ctagaagagt
     6121 tttcgctgaa caatgtgaaa acctaattcg caatgccaca ggaaactcat cgcattgtat
     6181 gaagctagaa caggtgctgg tattgcacaa aaagaaatat ggctaccaaa ttcagccgaa
     6241 aactttggga gtcatggata tggctacggc agttgagctg ttgccttacg tcgagctaaa
     6301 gaaaaaagaa caggccattt ggctcatctg tcacaacaac gatcatgaat ttcgttttct
     6361 ttgctatagg gtgtgtaagt atgtcatgga aagggaccct tctgcatcag tttggacaaa
     6421 cgttagaggt gaagttaagt taactaaatc tcaacaattc gtaaaaccaa ttgaaaaaag
     6481 catattggtt agggatttca atgctaagta taaagaccat ctttcagagt cagctctttt
     6541 agccatgcga caagcgattg aggtatatga tgatggtggc atacagtgta ttcgtcttac
     6601 ccgtttcatg aagtttataa ttgccattgt gcgtatgctt gaacagcggc caagcatgta
     6661 tctctatgag atcaagaatg gtttaaactg tggccttagc accacttttg aattcggatt
     6721 cccaaattta tattcagtgg ttgcagcaca taaagaccta ttcagtatta acaacggacc
     6781 aacgcaagaa agaagcgagg tttccattaa tatgaactgt gagcttcgtc aaagttcttt
     6841 aagcaaagac cagtgcgtcc tggaatccca gaagctaaca aattccaaac atagagcaca
     6901 acggagcttc catagtcaca ttggcgagca taatggactt ccaatcagtc agccccccct
     6961 gaaacgaagc gcaacaatca ataattgttc aacattccaa acatatcatg ctgataccga
     7021 catatttaat gtgccagcga attgtttacc gccaagaacc aacttatcat tgcctaatag
     7081 tttcggatca tctctggaaa gtggaagttt ttccaatagt tacagcaaca aggaaaactc
     7141 tatgaaatct cttcacttgt tcaacagttc atttaattcg tctggcgagc taaatgccag
     7201 tctaggcgca ggagatttaa caaatatatc tttagggaca ggtatcgcag ttgatacatc
     7261 aaatgaacca tccgaacttt ggcgtagacg tcaacaaaac tttaatcgcc ctaattcatc
     7321 aaattttcgt aaatcagata atggaactga cggtacttac accattggaa attacatcgc
     7381 accaccaata ggatccattt acgagccact aaagtataat ggtcaagaca aagagaaatt
     7441 acccttttgg attgatccca tttggagcaa aggatcggaa cagctcaggc atgacatact
     7501 taacattcga cttcctaagg ccagaacaac taaaacgttt tccaatatac tttcgcctta
     7561 caccatttcg aaaaatgaaa atttgaagcg acagttgttt agttttgata atcacgatcg
     7621 taaaatagct tgagcatgag atatccagca cagtggcggc cgctcgagtc tagagggccc
     7681 gcggttcgaa ggtaagccta tccctaaccc tctcctcggt ctcgattcta cgcgtaccgg
     7741 tcatcatcac catcaccatt gagtttaaac ccgctgatca gcctcgactg tgccttctaa
     7801 ggcctgagct cgctgatcag cctcgatcga ggatccagac atgataagat acattgatga
     7861 gtttggacaa accacaacta gaatgcagtg aaaaaaatgc tttatttgtg aaatttgtga
     7921 tgctattgct ttatttgtaa ccattataag ctgcaataaa caagttaaca acaacaattg
     7981 cattcatttt atgtttcagg ttcaggggga ggtgtgggag gttttttaaa gcaagtaaaa
     8041 cctctacaaa tgtggtatgg ctgattatga tcagtcgacc gatgcccttg agagccttca
     8101 acccagtcag ctccttccgg tgggcgcggg gcatgactat cgtcgccgca cttatgactg
     8161 tcttctttat catgcaactc gtaggacagg tgccggcagc gctctgggtc attttcggcg
     8221 aggaccgctt tcgctggagc gcgacgatga tcggcctgtc gcttgcggta ttcggaatct
     8281 tgcacgccct cgctcaagcc ttcgtcactg gtcccgccac caaacgtttc ggcgagaagc
     8341 aggccattat cgccggcatg gcggccgacg cgctgggcta cgtcttgctg gcgttcgcga
     8401 cgcgaggctg gatggccttc cccattatga ttcttctcgc ttccggcggc atcgggatgc
     8461 ccgcgttgca ggccatgctg tccaggcagg tagatgacga ccatcaggga cagcttcaag
     8521 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc
     8581 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
     8641 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
     8701 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
     8761 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
     8821 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
     8881 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
     8941 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca
     9001 ctagaaggac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag
     9061 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca
     9121 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg
     9181 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa
     9241 aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta
     9301 tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag
     9361 cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga
     9421 tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac
     9481 cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc
     9541 ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta
     9601 gttcgccagt taatagtttg cgcaacgttg ttgccattgc tgcaggcatc gtggtgtcac
     9661 gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat
     9721 gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa
     9781 gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg
     9841 tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag
     9901 aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aacacgggat aataccgcgc
     9961 cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct
    10021 caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat
    10081 cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg
    10141 ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc
    10201 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta
    10261 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg
    10321 tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc acgaggccct
    10381 ttcgt
//
