LOCUS       sequence-44314-e        2390 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2390)
  AUTHORS   Raj B, O'Hanlon D, Vessey JP, Pan Q, Ray D, Buckley NJ, Miller FD, 
            Blencowe BJ
  TITLE     Cross-regulation between an alternative splicing activator and a 
            transcription repressor controls neurogenesis.
  JOURNAL   Mol Cell. 2011 Sep 2;43(5):843-50.
  PUBMED    21884984
REFERENCE   2  (bases 1 to 2390)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell.";
            date: "2011-09-2"; volume: "43(5)"; pages: "843-50"
COMMENT     Sequence Label: pLDPuro-MmnSR100N
FEATURES             Location/Qualifiers
     source          1..2390
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(1879..1903)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1914..1979
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     misc_feature    2050..2167
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 atggactaca aagaccatga cggtgataca tatgatcatg acatcgatta caaggctagc
       61 ctgcagcagg gcgagaaaca gctgtttgag aagttctgga aagggacctt caaagcagtg
      121 gccactccgc gtcccgagag catcatcgtc gccagtatca cagcccgcaa gccgatgcca
      181 aggacagagc cccagagcag tctcttgctc ccagatcagg atggaccttc agaaaagctg
      241 ggccaacatc tggcccctga ggccctgggt accaacagct ggggaagaga gaaggcctgc
      301 agggaactgg atcctgccag agcacacagt gcctctcaag acagagaccc gacaccacca
      361 ccttcctcca gggggaaaaa gaaaaagaag aaatcaacca ggaaaaagag gaggaggtcc
      421 ccgtcctaca gcccatcacc agtcaagaag aagaagaaga aaagctccaa gaaacataag
      481 cgacacaggt ctttctccaa gaaaaggaga cacagctcct gtagccctaa aagcaaaaga
      541 cgggaagaga agaggcacaa aaaacaatct cgaagccgga agtctcaccg acatagacat
      601 caccgatgcc cctcaaggtc ccagagttcg gaactgcgct cccccagctg cgagagcagg
      661 catcgaggtc ggtcccctga ggaagggcgg aaatcccgcc gaacacactc ccgccgctgc
      721 tccaagaacc actgcaaggt cagcccagac gcccggtcca gccacctgcc cagccagccc
      781 ctcccgaggc tcggcttcct gtcagccagg ggtgtaatca ctgggtcggg gtccgcggct
      841 gacctcttta gcaaatcggc cagccctctc gccgccaccc gagggcgctc gcaggagtac
      901 gactcgggca atgacacgtc ctcgccgccc tccacgcaaa ccagctcggc caggtctcgg
      961 ggccaggaga aggggagtcc cggtggggat ctaagcaaga gtcgagacct caactgtggc
     1021 aacacctccg attcagggaa ctccttcacc acctcctccc cccagaacaa gggggctgtc
     1081 ttggagactg tctctcccgc ctgcaggagc agagagtcaa gagggtttca gtccccatgt
     1141 ctccagtgtg cagaggtgaa gaagtctagt ttggtcccat ccacagcccg gagctccccc
     1201 atcaaagagt gctcccgtag ctcctcctac accagcactc gatcctccag tccctcatcc
     1261 aggtccccaa accccagagc ttcccccagg tacacccgga gtcggtccac ctcctctgaa
     1321 aaaaggtcct actctcgctc tcccagctat tcgtccaagt ctggcaagag gagcccacct
     1381 agcagaagct ccaggtctcg tcgaagtccc agctactctc gctacagccc cagcagggaa
     1441 cgggacctaa agtatggtga gaaagaacca cagccccgag aacgtgcgcg caggaggcga
     1501 cggtcctact cacccatgag gaagcgcagg agagactccc caagccacct ggaggctcgg
     1561 aggataacca gtgcccgcaa acgccccatc ccctactacc gacccagccc ctcctcgtcc
     1621 agcagcctca gcagcgcctc ctcctggtac agcagcagca gcagcagcag cagcagtagc
     1681 agccgctccc ctagccgcag ttactcccgg agccggagtc ccagtcggag ccacagcagc
     1741 cggagccaga cccggagcag gacgcggacc agccgcagct ccagttctcg cagcctcagc
     1801 ctgggctccc ggagccgcag tcggaaccgg agcctgagct acagctcggc agaaagctat
     1861 gccagcacga ggcgctaaac ccagctttct tgtacaaagt ggttgattct agagactaca
     1921 aagaccatga cggtgattat aaagatcatg acatcgatta caaggatgac gatgacaagt
     1981 aaggatcccg ggtggcatcc ctgtgttcga attctcgacc tcgagacaaa tggcagtatt
     2041 catccacaat tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt
     2101 agacataata gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaaattca
     2161 aaattttcgg gtttattaca gggacagcag agatccactt tggccgcggc tcgagggggt
     2221 tggggttgcg ccttttccaa ggcagccctg ggtttgcgca gggacgcggc tgctctgggc
     2281 gtggttccgg gaaacgcagc ggcgccgacc ctgggtctcg cacattcttc acgtccgttc
     2341 gcagcgtcac ccggatcttc gccgctaccc ttgtgggccc cccggcgacg
//
