LOCUS       sequence-443146-        9795 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of xPE.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9795)
  AUTHORS   Chauhan VP, Sharp PA, Langer R
  TITLE     Engineered prime editors with minimal genomic errors.
  JOURNAL   Nature. 2025 Sep 17. doi: 10.1038/s41586-025-09537-3.
  PUBMED    40963020
REFERENCE   2  (bases 1 to 9795)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2025 Sep 17. doi: 10.1038/s41586-025-09537-3."
COMMENT     Sequence Label: pV231-xPE
FEATURES             Location/Qualifiers
     source          1..9795
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             2685..2705
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4806..4826
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4836..4862
                     /codon_start=1
                     /product="nuclear localization signal of human c-Myc 
                     proto-oncogene (Dang and Lee, 1988)"
                     /label=c-myc NLS
                     /translation="PAAKRVKLD"
     CDS             4871..4888
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(4911..4928)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4917..5141
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(5212..5228)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5212..5228)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5225..5247)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5236..5252
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5260..5266,5267..5284,5285..5290))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5305..5326
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5443..5460)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5614..6202)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5694..5713)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6373..7164,7165..7233))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6996..7015
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7234..7338)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(7417..7436)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        7608..7987
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        7988..8191
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     8142..8162
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     8233..8252
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        8233..8251
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             8301..8321
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             join(8322..9795,1..2627)
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRKLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLADDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYAHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTRLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
ORIGIN
        1 agcggatgac caacttcgat aagaacctgc ccaacgagaa ggtgctgccc aagcacagcc
       61 tgctgtacga gtacttcacc gtgtataacg agctgaccaa agtgaaatac gtgaccgagg
      121 gaatgagaaa gcccgccttc ctgagcggcg agcagaaaaa ggccatcgtg gacctgctgt
      181 tcaagaccaa ccggaaagtg accgtgaagc agctgaaaga ggactacttc aagaaaatcg
      241 agtgcttcga ctccgtggaa atctccggcg tggaagatcg gttcaacgcc tccctgggca
      301 cataccacga tctgctgaaa attatcaagg acaaggactt cctggacaat gaggaaaacg
      361 aggacattct ggaagatatc gtgctgaccc tgacactgtt tgaggacaga gagatgatcg
      421 aggaacggct gaaaacctat gcccacctgt tcgacgacaa agtgatgaag cagctgaagc
      481 ggcggagata caccggctgg ggcaggctga gccggaagct gatcaacggc atccgggaca
      541 agcagtccgg caagacaatc ctggatttcc tgaagtccga cggcttcgcc aacagaaact
      601 tcatgcagct gatccacgac gacagcctga cctttaaaga ggacatccag aaagcccagg
      661 tgtccggcca gggcgatagc ctgcacgagc acattgccaa tctggccggc agccccgcca
      721 ttaagaaggg catcctgcag acagtgaagg tggtggacga gctcgtgaaa gtgatgggcc
      781 ggcacaagcc cgagaacatc gtgatcgaaa tggccagaga gaaccagacc acccagaagg
      841 gacagaagaa cagccgcgag agaatgaagc ggatcgaaga gggcatcaaa gagctgggca
      901 gccagatcct gaaagaacac cccgtggaaa acacccagct gcagaacgag aagctgtacc
      961 tgtactacct gcagaatggg cgggatatgt acgtggacca ggaactggac atcaaccggc
     1021 tgtccgacta cgatgtggac gctatcgtgc ctcagagctt tctggccgac gactccatcg
     1081 acaacaaggt gctgaccaga agcgacaaga accggggcaa gagcgacaac gtgccctccg
     1141 aagaggtcgt gaagaagatg aagaactact ggcggcagct gctgaacgcc aagctgatta
     1201 cccagagaaa gttcgacaat ctgaccaagg ccgagagagg cggcctgagc gaactggata
     1261 aggccggctt catcaagaga cagctggtgg aaacccggca gatcacaaag cacgtggcac
     1321 agatcctgga ctcccggatg aacactaagt acgacgagaa tgacaagctg atccgggaag
     1381 tgaaagtgat caccctgaag tccaagctgg tgtccgattt ccggaaggat ttccagtttt
     1441 acaaagtgcg cgagatcaac aactacgccc acgcccacga cgcctacctg aacgccgtcg
     1501 tgggaaccgc cctgatcaaa aagtacccta agctggaaag cgagttcgtg tacggcgact
     1561 acaaggtgta cgacgtgcgg aagatgatcg ccaagagcga gcaggaaatc ggcaaggcta
     1621 ccgccaagta cttcttctac agcaacatca tgaacttttt caagaccgag attaccctgg
     1681 ccaacggcga gatccggaag cggcctctga tcgagacaaa cggcgaaacc ggggagatcg
     1741 tgtgggataa gggccgggat tttgccaccg tgcggaaagt gctgagcatg ccccaagtga
     1801 atatcgtgaa aaagaccgag gtgcagacag gcggcttcag caaagagtct atcctgccca
     1861 agaggaacag cgataagctg atcgccagaa agaaggactg ggaccctaag aagtacggcg
     1921 gcttcgacag ccccaccgtg gcctattctg tgctggtggt ggccaaagtg gaaaagggca
     1981 agtccaagaa actgaagagt gtgaaagagc tgctggggat caccatcatg gaaagaagca
     2041 gcttcgagaa gaatcccatc gactttctgg aagccaaggg ctacaaagaa gtgaaaaagg
     2101 acctgatcat caagctgcct aagtactccc tgttcgagct ggaaaacggc cggaagagaa
     2161 tgctggcctc tgccggcgaa ctgcagaagg gaaacgaact ggccctgccc tccaaatatg
     2221 tgaacttcct gtacctggcc agccactatg agaagctgaa gggctccccc gaggataatg
     2281 agcagaaaca gctgtttgtg gaacagcaca agcactacct ggacgagatc atcgagcaga
     2341 tcagcgagtt ctccaagaga gtgatcctgg ccgacgctaa tctggacaaa gtgctgtccg
     2401 cctacaacaa gcaccgggat aagcccatca gagagcaggc cgagaatatc atccacctgt
     2461 ttaccctgac ccggctggga gcccctgccg ccttcaagta ctttgacacc accatcgacc
     2521 ggaagaggta caccagcacc aaagaggtgc tggacgccac cctgatccac cagagcatca
     2581 ccggcctgta cgagacacgg atcgacctgt ctcagctggg aggtgactcc ggcggaagct
     2641 ctggtggcag caagcggacc gccgacggct ctgaattcga gagccctaag aagaaaagaa
     2701 aggtgagcgg aggctctagc ggcggaagca ccctgaacat tgaagacgag tatagactgc
     2761 atgaaacaag caaggaaccc gacgtgtccc tgggctccac ctggctgtcc gactttcccc
     2821 aggcctgggc cgagacagga ggaatgggcc tggccgtgcg gcaggcaccc ctgatcatcc
     2881 ctctgaaggc cacctctaca cccgtgagca tcaagcagta ccctatgtct caggaggcca
     2941 gactgggcat caagcctcac atccagaggc tgctggacca gggcatcctg gtgccatgcc
     3001 agagcccctg gaacacacca ctgctgcccg tgaagaagcc aggcaccaat gactatagac
     3061 ccgtgcagga tctgagagag gtgaacaaga gggtggagga tatccacccc accgtgccca
     3121 acccttacaa tctgctgtcc ggcctgcccc cttctcacca gtggtataca gtgctggacc
     3181 tgaaggatgc cttcttttgt ctgagactgc accctaccag ccagccactg ttcgcctttg
     3241 agtggaggga ccctgagatg ggcatctctg gccagctgac ctggacacgc ctgcctcagg
     3301 gcttcaagaa tagcccaaca ctgtttaacg aggccctgca ccgcgacctg gcagatttcc
     3361 ggatccagca cccagatctg atcctgctgc agtacgtgga cgatctgctg ctggccgcca
     3421 ccagcgagct ggattgccag cagggaacac gcgccctgct gcagaccctg ggaaacctgg
     3481 gatatagggc atccgccaag aaggcccaga tctgtcagaa gcaggtgaag tacctgggct
     3541 atctgctgaa ggagggccag agatggctga cagaggccag gaaggagaca gtgatgggcc
     3601 agccaacacc caagacccca agacagctga gggagttcct gggcaaagca ggattttgca
     3661 ggctgttcat cccaggattc gcagagatgg cagcacctct gtacccactg accaagccgg
     3721 gcaccctgtt taattggggc cctgaccagc agaaggccta tcaggagatc aagcaggccc
     3781 tgctgacagc accagccctg ggcctgccag acctgaccaa gcctttcgag ctgtttgtgg
     3841 atgagaagca gggctacgcc aagggcgtgc tgacccagaa gctgggacca tggagacggc
     3901 ccgtggccta tctgtccaag aagctggacc cagtggcagc aggatggcca ccatgcctga
     3961 ggatggtggc agcaatcgcc gtgctgacaa aggatgccgg caagctgacc atgggacagc
     4021 cactggtcat cctggcacca cacgcagtgg aggccctggt gaagcagcct ccagatcgct
     4081 ggctgtctaa cgcccggatg acacactacc aggccctgct gctggacacc gatcgcgtgc
     4141 agtttggccc tgtggtggcc ctgaatccag ccaccctgct gcctctgcca gaggagggcc
     4201 tgcagcacaa ctgtctggac atcctggcag aggcacacgg aacaaggcca gacctgaccg
     4261 atcagcccct gcctgacgcc gatcacacat ggtataccga tggaagctcc ctgctgcagg
     4321 agggccagag gaaggcagga gcagcagtga ccacagagac agaagtgatc tgggccaagg
     4381 ccctgccagc aggcacatcc gcccagcggg ccgagctgat cgccctgacc caggccctga
     4441 agatggccga gggcaagaag ctgaacgtgt acacagactc cagatatgcc ttcgccaccg
     4501 cacacatcca cggagagatc tacaggcgcc ggggctggct gacctctgag ggcaaggaga
     4561 tcaagaacaa ggatgagatc ctggccctgc tgaaggccct gtttctgccc aagcggctga
     4621 gcatcatcca ctgtcctgga caccagaagg gacactccgc cgaggcaagg ggcaatcgga
     4681 tggccgacca ggccgccaga aaggctgcta ttactgaaac tcccgacact tccactctgc
     4741 tgattgaaaa ctcctcccct tctggcggct caaaaagaac cgccgacggc agcgaattcg
     4801 agtctcccaa gaagaagagg aaagtcggct ctggccctgc cgctaagaga gtgaagctgg
     4861 actaaccggt catcatcacc atcaccattg agtttaaacc cgctgatcag cctcgactgt
     4921 gccttctagt tgccagccat ctgttgtttg cccctccccc gtgccttcct tgaccctgga
     4981 aggtgccact cccactgtcc tttcctaata aaatgagaaa attgcatcgc attgtctgag
     5041 taggtgtcat tctattctgg ggggtggggt ggggcaggac agcaaggggg aggattggga
     5101 agacaatagc aggcatgctg gggatgcggt gggctctatg gcttctgagg cggaaagaac
     5161 cagctggggc tcgataccgt cgacctctag ctagagcttg gcgtaatcat ggtcatagct
     5221 gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat
     5281 aaagtgtaaa gcctagggtg cctaatgagt gagctaactc acattaattg cgttgcgctc
     5341 actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg
     5401 cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct
     5461 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt
     5521 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc
     5581 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga
     5641 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata
     5701 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac
     5761 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg
     5821 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc
     5881 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag
     5941 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt
     6001 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt
     6061 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg
     6121 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac
     6181 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacactca
     6241 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac
     6301 ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac
     6361 ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt
     6421 tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt
     6481 accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt
     6541 atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc
     6601 cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa
     6661 tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg
     6721 tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt
     6781 gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc
     6841 agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt
     6901 aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg
     6961 gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac
     7021 tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc
     7081 gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt
     7141 tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg
     7201 aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat attattgaag
     7261 catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa
     7321 acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtcg acggatcggg
     7381 agatcgatct cccgatcccc tagggtcgac tctcagtaca atctgctctg atgccgcata
     7441 gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt gcgcgagcaa
     7501 aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc tgcttagggt
     7561 taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac attgattatt
     7621 gactagttat taatagtaat caattacggg gtcattagtt catagcccat atatggagtt
     7681 ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg acccccgccc
     7741 attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt tccattgacg
     7801 tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag tgtatcatat
     7861 gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc attatgccca
     7921 gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag tcatcgctat
     7981 taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt ttgactcacg
     8041 gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc accaaaatca
     8101 acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg gcggtaggcg
     8161 tgtacggtgg gaggtctata taagcagagc tggtttagtg aaccgtcaga tccgctagag
     8221 atccgcggcc gctaatacga ctcactatag ggagagccgc caccatgaaa cggacagccg
     8281 acggaagcga gttcgagtca ccaaagaaga agcggaaagt cgacaagaag tacagcatcg
     8341 gcctggacat cggcaccaac tctgtgggct gggccgtgat caccgacgag tacaaggtgc
     8401 ccagcaagaa attcaaggtg ctgggcaaca ccgaccggca cagcatcaag aagaacctga
     8461 tcggagccct gctgttcgac agcggcgaaa cagccgaggc cacccggctg aagagaaccg
     8521 ccagaagaag atacaccaga cggaagaacc ggatctgcta tctgcaagag atcttcagca
     8581 acgagatggc caaggtggac gacagcttct tccacagact ggaagagtcc ttcctggtgg
     8641 aagaggataa gaagcacgag cggcacccca tcttcggcaa catcgtggac gaggtggcct
     8701 accacgagaa gtaccccacc atctaccacc tgagaaagaa actggtggac agcaccgaca
     8761 aggccgacct gcggctgatc tatctggccc tggcccacat gatcaagttc cggggccact
     8821 tcctgatcga gggcgacctg aaccccgaca acagcgacgt ggacaagctg ttcatccagc
     8881 tggtgcagac ctacaaccag ctgttcgagg aaaaccccat caacgccagc ggcgtggacg
     8941 ccaaggccat cctgtctgcc agactgagca agagcagaaa gctggaaaat ctgatcgccc
     9001 agctgcccgg cgagaagaag aatggcctgt tcggaaacct gattgccctg agcctgggcc
     9061 tgacccccaa cttcaagagc aacttcgacc tggccgagga tgccaaactg cagctgagca
     9121 aggacaccta cgacgacgac ctggacaacc tgctggccca gatcggcgac cagtacgccg
     9181 acctgtttct ggccgccaag aacctgtccg acgccatcct gctgagcgac atcctgagag
     9241 tgaacaccga gatcaccaag gcccccctga gcgcctctat gatcaagaga tacgacgagc
     9301 accaccagga cctgaccctg ctgaaagctc tcgtgcggca gcagctgcct gagaagtaca
     9361 aagagatttt cttcgaccag agcaagaacg gctacgccgg ctacattgac ggcggagcca
     9421 gccaggaaga gttctacaag ttcatcaagc ccatcctgga aaagatggac ggcaccgagg
     9481 aactgctcgt gaagctgaac agagaggacc tgctgcggaa gcagcggacc ttcgacaacg
     9541 gcagcatccc ccaccagatc cacctgggag agctgcacgc cattctgcgg cggcaggaag
     9601 atttttaccc attcctgaag gacaaccggg aaaagatcga gaagatcctg accttccgca
     9661 tcccctacta cgtgggccct ctggccaggg gaaacagcag attcgcctgg atgaccagaa
     9721 agagcgagga aaccatcacc ccctggaact tcgaggaagt ggtggacaag ggcgcttccg
     9781 cccagagctt catcg
//
