LOCUS       sequence-443147-       10473 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of vPE.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10473)
  AUTHORS   Chauhan VP, Sharp PA, Langer R
  TITLE     Engineered prime editors with minimal genomic errors.
  JOURNAL   Nature. 2025 Sep 17. doi: 10.1038/s41586-025-09537-3.
  PUBMED    40963020
REFERENCE   2  (bases 1 to 10473)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2025 Sep 17. doi: 10.1038/s41586-025-09537-3."
COMMENT     Sequence Label: pV238-vPE
FEATURES             Location/Qualifiers
     source          1..10473
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        220..423
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     374..394
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     465..484
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        465..483
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             533..553
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             554..4654
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRKLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLADDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYAHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTRLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             4712..4732
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             7511..7531
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             7541..7567
                     /codon_start=1
                     /product="nuclear localization signal of human c-Myc 
                     proto-oncogene (Dang and Lee, 1988)"
                     /label=c-myc NLS
                     /translation="PAAKRVKLD"
     CDS             7576..7593
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(7616..7633)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7622..7846
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(7917..7933)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7917..7933)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7930..7952)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7941..7957
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7965..7971,7972..7989,7990..7995))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8010..8031
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8148..8165)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8319..8907)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8399..8418)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9078..9869,9870..9938))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9701..9720
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9939..10043)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(10122..10141)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        join(10313..10473,1..219)
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 atagtaacgc caatagggac tttccattga cgtcaatggg tggagtattt acggtaaact
       61 gcccacttgg cagtacatca agtgtatcat atgccaagta cgccccctat tgacgtcaat
      121 gacggtaaat ggcccgcctg gcattatgcc cagtacatga ccttatggga ctttcctact
      181 tggcagtaca tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac
      241 atcaatgggc gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac
      301 gtcaatggga gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac
      361 tccgccccat tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta tataagcaga
      421 gctggtttag tgaaccgtca gatccgctag agatccgcgg ccgctaatac gactcactat
      481 agggagagcc gccaccatga aacggacagc cgacggaagc gagttcgagt caccaaagaa
      541 gaagcggaaa gtcgacaaga agtacagcat cggcctggac atcggcacca actctgtggg
      601 ctgggccgtg atcaccgacg agtacaaggt gcccagcaag aaattcaagg tgctgggcaa
      661 caccgaccgg cacagcatca agaagaacct gatcggagcc ctgctgttcg acagcggcga
      721 aacagccgag gccacccggc tgaagagaac cgccagaaga agatacacca gacggaagaa
      781 ccggatctgc tatctgcaag agatcttcag caacgagatg gccaaggtgg acgacagctt
      841 cttccacaga ctggaagagt ccttcctggt ggaagaggat aagaagcacg agcggcaccc
      901 catcttcggc aacatcgtgg acgaggtggc ctaccacgag aagtacccca ccatctacca
      961 cctgagaaag aaactggtgg acagcaccga caaggccgac ctgcggctga tctatctggc
     1021 cctggcccac atgatcaagt tccggggcca cttcctgatc gagggcgacc tgaaccccga
     1081 caacagcgac gtggacaagc tgttcatcca gctggtgcag acctacaacc agctgttcga
     1141 ggaaaacccc atcaacgcca gcggcgtgga cgccaaggcc atcctgtctg ccagactgag
     1201 caagagcaga aagctggaaa atctgatcgc ccagctgccc ggcgagaaga agaatggcct
     1261 gttcggaaac ctgattgccc tgagcctggg cctgaccccc aacttcaaga gcaacttcga
     1321 cctggccgag gatgccaaac tgcagctgag caaggacacc tacgacgacg acctggacaa
     1381 cctgctggcc cagatcggcg accagtacgc cgacctgttt ctggccgcca agaacctgtc
     1441 cgacgccatc ctgctgagcg acatcctgag agtgaacacc gagatcacca aggcccccct
     1501 gagcgcctct atgatcaaga gatacgacga gcaccaccag gacctgaccc tgctgaaagc
     1561 tctcgtgcgg cagcagctgc ctgagaagta caaagagatt ttcttcgacc agagcaagaa
     1621 cggctacgcc ggctacattg acggcggagc cagccaggaa gagttctaca agttcatcaa
     1681 gcccatcctg gaaaagatgg acggcaccga ggaactgctc gtgaagctga acagagagga
     1741 cctgctgcgg aagcagcgga ccttcgacaa cggcagcatc ccccaccaga tccacctggg
     1801 agagctgcac gccattctgc ggcggcagga agatttttac ccattcctga aggacaaccg
     1861 ggaaaagatc gagaagatcc tgaccttccg catcccctac tacgtgggcc ctctggccag
     1921 gggaaacagc agattcgcct ggatgaccag aaagagcgag gaaaccatca ccccctggaa
     1981 cttcgaggaa gtggtggaca agggcgcttc cgcccagagc ttcatcgagc ggatgaccaa
     2041 cttcgataag aacctgccca acgagaaggt gctgcccaag cacagcctgc tgtacgagta
     2101 cttcaccgtg tataacgagc tgaccaaagt gaaatacgtg accgagggaa tgagaaagcc
     2161 cgccttcctg agcggcgagc agaaaaaggc catcgtggac ctgctgttca agaccaaccg
     2221 gaaagtgacc gtgaagcagc tgaaagagga ctacttcaag aaaatcgagt gcttcgactc
     2281 cgtggaaatc tccggcgtgg aagatcggtt caacgcctcc ctgggcacat accacgatct
     2341 gctgaaaatt atcaaggaca aggacttcct ggacaatgag gaaaacgagg acattctgga
     2401 agatatcgtg ctgaccctga cactgtttga ggacagagag atgatcgagg aacggctgaa
     2461 aacctatgcc cacctgttcg acgacaaagt gatgaagcag ctgaagcggc ggagatacac
     2521 cggctggggc aggctgagcc ggaagctgat caacggcatc cgggacaagc agtccggcaa
     2581 gacaatcctg gatttcctga agtccgacgg cttcgccaac agaaacttca tgcagctgat
     2641 ccacgacgac agcctgacct ttaaagagga catccagaaa gcccaggtgt ccggccaggg
     2701 cgatagcctg cacgagcaca ttgccaatct ggccggcagc cccgccatta agaagggcat
     2761 cctgcagaca gtgaaggtgg tggacgagct cgtgaaagtg atgggccggc acaagcccga
     2821 gaacatcgtg atcgaaatgg ccagagagaa ccagaccacc cagaagggac agaagaacag
     2881 ccgcgagaga atgaagcgga tcgaagaggg catcaaagag ctgggcagcc agatcctgaa
     2941 agaacacccc gtggaaaaca cccagctgca gaacgagaag ctgtacctgt actacctgca
     3001 gaatgggcgg gatatgtacg tggaccagga actggacatc aaccggctgt ccgactacga
     3061 tgtggacgct atcgtgcctc agagctttct ggccgacgac tccatcgaca acaaggtgct
     3121 gaccagaagc gacaagaacc ggggcaagag cgacaacgtg ccctccgaag aggtcgtgaa
     3181 gaagatgaag aactactggc ggcagctgct gaacgccaag ctgattaccc agagaaagtt
     3241 cgacaatctg accaaggccg agagaggcgg cctgagcgaa ctggataagg ccggcttcat
     3301 caagagacag ctggtggaaa cccggcagat cacaaagcac gtggcacaga tcctggactc
     3361 ccggatgaac actaagtacg acgagaatga caagctgatc cgggaagtga aagtgatcac
     3421 cctgaagtcc aagctggtgt ccgatttccg gaaggatttc cagttttaca aagtgcgcga
     3481 gatcaacaac tacgcccacg cccacgacgc ctacctgaac gccgtcgtgg gaaccgccct
     3541 gatcaaaaag taccctaagc tggaaagcga gttcgtgtac ggcgactaca aggtgtacga
     3601 cgtgcggaag atgatcgcca agagcgagca ggaaatcggc aaggctaccg ccaagtactt
     3661 cttctacagc aacatcatga actttttcaa gaccgagatt accctggcca acggcgagat
     3721 ccggaagcgg cctctgatcg agacaaacgg cgaaaccggg gagatcgtgt gggataaggg
     3781 ccgggatttt gccaccgtgc ggaaagtgct gagcatgccc caagtgaata tcgtgaaaaa
     3841 gaccgaggtg cagacaggcg gcttcagcaa agagtctatc ctgcccaaga ggaacagcga
     3901 taagctgatc gccagaaaga aggactggga ccctaagaag tacggcggct tcgacagccc
     3961 caccgtggcc tattctgtgc tggtggtggc caaagtggaa aagggcaagt ccaagaaact
     4021 gaagagtgtg aaagagctgc tggggatcac catcatggaa agaagcagct tcgagaagaa
     4081 tcccatcgac tttctggaag ccaagggcta caaagaagtg aaaaaggacc tgatcatcaa
     4141 gctgcctaag tactccctgt tcgagctgga aaacggccgg aagagaatgc tggcctctgc
     4201 cggcgaactg cagaagggaa acgaactggc cctgccctcc aaatatgtga acttcctgta
     4261 cctggccagc cactatgaga agctgaaggg ctcccccgag gataatgagc agaaacagct
     4321 gtttgtggaa cagcacaagc actacctgga cgagatcatc gagcagatca gcgagttctc
     4381 caagagagtg atcctggccg acgctaatct ggacaaagtg ctgtccgcct acaacaagca
     4441 ccgggataag cccatcagag agcaggccga gaatatcatc cacctgttta ccctgacccg
     4501 gctgggagcc cctgccgcct tcaagtactt tgacaccacc atcgaccgga agaggtacac
     4561 cagcaccaaa gaggtgctgg acgccaccct gatccaccag agcatcaccg gcctgtacga
     4621 gacacggatc gacctgtctc agctgggagg tgactccggc ggaagctctg gtggcagcaa
     4681 gcggaccgcc gacggctctg aattcgagag ccctaagaag aaaagaaagg tgagcggagg
     4741 ctctagcggc ggaagcaccc tgaacattga agacgagtat agactgcatg aaacaagcaa
     4801 ggaacccgac gtgtccctgg gctccacctg gctgtccgac tttccccagg cctgggccga
     4861 gacaggagga atgggcctgg ccgtgcggca ggcacccctg atcatccctc tgaaggccac
     4921 ctctacaccc gtgagcatca agcagtaccc tatgtctcag gaggccagac tgggcatcaa
     4981 gcctcacatc cagaggctgc tggaccaggg catcctggtg ccatgccaga gcccctggaa
     5041 cacaccactg ctgcccgtga agaagccagg caccaatgac tatagacccg tgcaggatct
     5101 gagagaggtg aacaagaggg tggaggatat ccaccccacc gtgcccaacc cttacaatct
     5161 gctgtccggc ctgccccctt ctcaccagtg gtatacagtg ctggacctga aggatgcctt
     5221 cttttgtctg agactgcacc ctaccagcca gccactgttc gcctttgagt ggagggaccc
     5281 tgagatgggc atctctggcc agctgacctg gacacgcctg cctcagggct tcaagaatag
     5341 cccaacactg tttaacgagg ccctgcaccg cgacctggca gatttccgga tccagcaccc
     5401 agatctgatc ctgctgcagt acgtggacga tctgctgctg gccgccacca gcgagctgga
     5461 ttgccagcag ggaacacgcg ccctgctgca gaccctggga aacctgggat atagggcatc
     5521 cgccaagaag gcccagatct gtcagaagca ggtgaagtac ctgggctatc tgctgaagga
     5581 gggccagaga tggctgacag aggccaggaa ggagacagtg atgggccagc caacacccaa
     5641 gaccccaaga cagctgaggg agttcctggg caaagcagga ttttgcaggc tgttcatccc
     5701 aggattcgca gagatggcag cacctctgta cccactgacc aagccgggca ccctgtttaa
     5761 ttggggccct gaccagcaga aggcctatca ggagatcaag caggccctgc tgacagcacc
     5821 agccctgggc ctgccagacc tgaccaagcc tttcgagctg tttgtggatg agaagcaggg
     5881 ctacgccaag ggcgtgctga cccagaagct gggaccatgg agacggcccg tggcctatct
     5941 gtccaagaag ctggacccag tggcagcagg atggccacca tgcctgagga tggtggcagc
     6001 aatcgccgtg ctgacaaagg atgccggcaa gctgaccatg ggacagccac tggtcatcct
     6061 ggcaccacac gcagtggagg ccctggtgaa gcagcctcca gatcgctggc tgtctaacgc
     6121 ccggatgaca cactaccagg ccctgctgct ggacaccgat cgcgtgcagt ttggccctgt
     6181 ggtggccctg aatccagcca ccctgctgcc tctgccagag gagggcctgc agcacaactg
     6241 tctggacatc ctggcagagg cacacggaac aaggccagac ctgaccgatc agcccctgcc
     6301 tgacgccgat cacacatggt ataccgatgg aagctccctg ctgcaggagg gccagaggaa
     6361 ggcaggagca gcagtgacca cagagacaga agtgatctgg gccaaggccc tgccagcagg
     6421 cacatccgcc cagcgggccg agctgatcgc cctgacccag gccctgaaga tggccgaggg
     6481 caagaagctg aacgtgtaca cagactccag atatgccttc gccaccgcac acatccacgg
     6541 agagatctac aggcgccggg gctggctgac ctctgagggc aaggagatca agaacaagga
     6601 tgagatcctg gccctgctga aggccctgtt tctgcccaag cggctgagca tcatccactg
     6661 tcctggacac cagaagggac actccgccga ggcaaggggc aatcggatgg ccgaccaggc
     6721 cgccagaaag gctgctatta ctgaaactcc cgacacttcc actctgctga ttgaaaactc
     6781 ctccccttcc ggaggatcta gcggaggctc ctctggctct gagacacctg gcacaagcga
     6841 gagcgcaaca cctgaaagca gcgggggcag cagcgggggg tcaatggctg aaaatggtga
     6901 taatgaaaag atggctgccc tggaggccaa aatctgtcat caaattgagt attattttgg
     6961 cgacttcaat ttgccacggg acaagtttct aaaggaacag ataaaactgg atgaaggctg
     7021 ggtacctttg gagataatga taaaattcaa caggttgaac cgtctaacaa cagactttaa
     7081 tgtaattgtg gaagcattga gcaaatccaa ggcagaactc atggaaatca gtgaagataa
     7141 aactaaaatc agaaggtctc caagcaaacc cctacctgaa gtgactgatg agtataaaaa
     7201 tgatgtaaaa aacagatctg tttatattaa aggcttccca actgatgcaa ctcttgatga
     7261 cataaaagaa tggttagaag ataaaggtca agtactaaat attcagatga gaagaacatt
     7321 gcataaagca tttaagggat caatttttgt tgtgtttgat agcattgaat ctgctaagaa
     7381 atttgtagag acccctggcc agaagtacaa agaaacagac ctgctaatac ttttcaagga
     7441 cgattacttt gccaaaaaaa atgaatctgg cggctcaaaa agaaccgccg acggcagcga
     7501 attcgagtct cccaagaaga agaggaaagt cggctctggc cctgccgcta agagagtgaa
     7561 gctggactaa ccggtcatca tcaccatcac cattgagttt aaacccgctg atcagcctcg
     7621 actgtgcctt ctagttgcca gccatctgtt gtttgcccct cccccgtgcc ttccttgacc
     7681 ctggaaggtg ccactcccac tgtcctttcc taataaaatg agaaaattgc atcgcattgt
     7741 ctgagtaggt gtcattctat tctggggggt ggggtggggc aggacagcaa gggggaggat
     7801 tgggaagaca atagcaggca tgctggggat gcggtgggct ctatggcttc tgaggcggaa
     7861 agaaccagct ggggctcgat accgtcgacc tctagctaga gcttggcgta atcatggtca
     7921 tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga
     7981 agcataaagt gtaaagccta gggtgcctaa tgagtgagct aactcacatt aattgcgttg
     8041 cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc
     8101 caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac
     8161 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata
     8221 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa
     8281 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct
     8341 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
     8401 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
     8461 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca
     8521 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
     8581 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
     8641 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
     8701 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga
     8761 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc
     8821 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag
     8881 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac
     8941 actcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc
     9001 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag
     9061 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt
     9121 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag
     9181 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca
     9241 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact
     9301 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca
     9361 gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg
     9421 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc
     9481 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg
     9541 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca
     9601 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt
     9661 atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc
     9721 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc
     9781 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca
     9841 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa
     9901 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat
     9961 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa
    10021 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtcgacgga
    10081 tcgggagatc gatctcccga tcccctaggg tcgactctca gtacaatctg ctctgatgcc
    10141 gcatagttaa gccagtatct gctccctgct tgtgtgttgg aggtcgctga gtagtgcgcg
    10201 agcaaaattt aagctacaac aaggcaaggc ttgaccgaca attgcatgaa gaatctgctt
    10261 agggttaggc gttttgcgct gcttcgcgat gtacgggcca gatatacgcg ttgacattga
    10321 ttattgacta gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg
    10381 gagttccgcg ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc
    10441 cgcccattga cgtcaataat gacgtatgtt ccc
//
