LOCUS       sequence-443164-        7790 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression vector for expression of hAT-9_XT transposase 
            (CMV promoter).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7790)
  AUTHORS   Zhang T, Tan S, Tang N, Li Y, Zhang C, Sun J, Guo Y, Gao H, Cai Y, 
            Sun W, Wang C, Fu L, Ma H, Wu Y, Hu X, Zhang X, Gee P, Yan W, Zhao 
            Y, Chen Q, Guo B, Wang H, Zhang YE
  TITLE     Heterologous survey of 130 DNA transposons in human cells highlights
            their functional divergence and expands the genome engineering 
            toolbox.
  JOURNAL   Cell. 2024 Jul 11;187(14):3741-3760.e30. doi: 
            10.1016/j.cell.2024.05.007. Epub 2024 Jun 5.
  PUBMED    38843831
REFERENCE   2  (bases 1 to 7790)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2024.05.007"; journalName: "Cell"; date: 
            "2024-07-11- 11"; volume: "187"; issue: "14"; pages: "3741-3760.e30"
COMMENT     Sequence Label: pCMV-hAT-9_XT
FEATURES             Location/Qualifiers
     source          1..7790
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     629..648
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        629..647
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(3978..3996)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(3979..3996)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(4016..4033)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4022..4246
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4292..4720
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4379..4398)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4589..4610
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4729..4749)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4734..5063
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4914..5049
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     4976..4995
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     polyA_signal    5229..5350
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5266..5285)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5320..5339
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(5399..5415)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5399..5415)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5412..5434)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5423..5439
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5447..5453,5454..5471,5472..5477))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5492..5513
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5630..5647)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5801..6389)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5881..5900)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6560..7351,7352..7420))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7183..7202
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7421..7525)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(7600..7619)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctctctgg ctaactagag
      601 aacccactgc ttactggctt atcgaaatta atacgactca ctatagggag acccaagctt
      661 ggtaccgagc tcggatccac tagtaacggc cgccagtgtg ctggaattcg ccgccaccat
      721 gcctggtaaa ggccgtcctc ctcgtcgtgg tactcgtggt cgtgcggcga tgatttcatg
      781 cggccctcgt aaattaccga gctttccgaa ggcgctgacc ctgaatagcc agaacgccga
      841 ggaagtggtg gattggctga cccaacacac tcctagcagc accgtgagca actttgcgac
      901 caccagcagc agcagctcag caatgggtac ccctcgtact accagctcaa ccacagcggc
      961 acctagcagc ttagaaagcg aagagctgtt cacccatgaa tttgtggaac tgagcgatgc
     1021 gcaacctctg ctgcctgaag aggatgaggg cgatgaggat gtgacccctg atattatcat
     1081 tcaggcgggc aacaccaccg aaatggatat ccgctgcgat gaagttcctg cggcggccgt
     1141 tttttgtgag ctgagcgaag aaatcgatgc cagcgaggaa aacgatgatg aggagatcga
     1201 tattctgtgg gtgcctaccc gtcgtgaaca ggaagaagac agcagcgatg gcgaaaccga
     1261 aagccaacgt ggtcgtcgtc gtatccgttt acgtcgcagc cgtgatagct cacaaggtac
     1321 cgtgggccaa caacatgaaa gcgcgcctgt ggttagccaa ccgacccatc ctcctaccag
     1381 cactgtgacc gcgcgtaaac ctaccagcaa aggttcagcc gtgtggcact tttttaacgt
     1441 gtgcgcgagc gataaaagcg ccgtgatttg caacgagtgc agccagaaac tgagcctggg
     1501 caaacctaac acccatctgg gtacgactgc aatgcgtcgc cacatgaacg cgaagcacaa
     1561 agcgctgtgg gagcagcatc tgaaaggcag cagccagacc ttaagccatc ctcctagcgc
     1621 gcctgcaagc tattgtagca ccagcgccgt tctggatcct agccaacctc ctagcactcc
     1681 tcctagcacc ttaaccacca gcagctgtag ctcagcccct agccaagtta gcgtgcgtgc
     1741 gatgtttgag cgcaaaaagc cgatgcctcc tagccatcct ttagcacgtc gtttaaccgc
     1801 gggtctgagc gcattattag cgcgtcaact gctgccttat cagctggtgg atagcgaagc
     1861 cttccaccag tttgtggcga tcggtacccc tcagtggaaa gttccgagcc gcaacttctt
     1921 tagccagaaa ggcatcccgc atctgtatca gcacgtgcag agccaagtga ccgccagctt
     1981 aagctttagc gtgggcgcca aagtgcatat gaccaccgat acctggagca gcaaacatgg
     2041 ccagggccgc tatgttacct ataccgccca ttgggtgaac ttagtgatgg ccggcaaaca
     2101 aggtatgcgt ggcagcacca ccgttgagtt agtgagccct cctcgtgttg catgtggttc
     2161 agcgaccact agcactcctc cgttactgag caacagcagc agcaagagca gcagcaacag
     2221 cagcagcaat ctggcgtcaa gcagcgcaag cagcagctca gcagcagttt caagcagcac
     2281 ccctgtgcat cctcagctgc atatcggcta tagcacctgc caggtgcgtc gttgtcatgc
     2341 ggttctgggt atgacctgcc tggaaagccg taaccatacc ggtagcgcac tgttaagcag
     2401 cctgcatagc caagcggatc gttggttaac ccctcaccag ctgaagatcg gcaaggtggt
     2461 gtgcgataac ggcagcaacc tgttagccgc gctgcgttta ggtaacctga cccacgtgcc
     2521 ttgtatggcc catgtgctga acctgattgt gcagcgcttc gtgagcaagt atcctggcct
     2581 gcaggatatt ttacgccaga gccgcaaagt gagcggtcat ttccgccgta gctatactgc
     2641 aatggcccgc ttagcggata ttcagcgccg ccataattta ccggtgcgcc gcttaatatg
     2701 cgatagccag acccgctgga atagcaccct gatgatgttc gagcgcctgt tacaacaaca
     2761 gcgcgcggtg aacgagtatc tgttcgagtt aggcggtcgt accggtagcg cggaactggg
     2821 cattttcttt ccgcgctact gggtgttaat gcgcgatgcc tgccgcttaa tgcgtccttt
     2881 tgatgaggtg accaacatgg tgagccgtac cgaaggcacc attagcgatc tgatcccttt
     2941 cgcctttttt ctggagcgcg ccgttcgtcg tgttactgat gacgcggtgg atcaacgtga
     3001 tcaggagcac gatttctggg ccgaaagccc ggaacgtagc caagcacctg cagcgaccca
     3061 aggtgaagtt agcgaagtgg aaagcgaaga agagggcggc ttcgttgaag cggaagacca
     3121 acaggaacaa gcaagccgtg gtgcgtgcgg tcatttatta tggagccctg gtctggttcg
     3181 tggctggggt gaagaaaccg tggtggatga ggatgtggtg ctggataacg aggagggcga
     3241 gatggatact agcgccagca acttagttcg catgggcagc tttatgctga gctgcctgtt
     3301 aaaagacccg cgcatcaaac gcctgaaaga gaaagacctg tattgggtgg cgaccttatt
     3361 agaccctcgc tacaaacata aggtggccga aatgctgccg acctatcata aaagcgagcg
     3421 catgctgcac tttcagacca gcctgcagaa catgctgtat aacgccttca agggcgatgt
     3481 gacccctcat agccgtggtc gtggtgcggg taatcctcct actagcaccc ctgcacgtac
     3541 catgcatttt ggccacagcg tgacctcaga tatgcagagc ttttttagcc cgcgccagcg
     3601 tcaagatcct agcggcagca ccttacgcga acgtctggat cgtcaggttg cggattattt
     3661 agcgctgacc gccgatattg ataccctgcg cagcgatgag ccgttagact attgggtgcg
     3721 tcgcctggat ttatggcctg agctgagcca gtttgcgatc aacctgctga gctgccctgc
     3781 aagcagcgtt ttaagcgaac gcaccttttc agcagcgggc ggcattgtta ccgaaaagcg
     3841 tacccgcctg ggccataaaa gcgtggacta cctgaccttt atcaaaatga acgaggcctg
     3901 gattagcgaa ggctattgca ccccggaaga tctgttctga tgagcggccg ctcgagcatg
     3961 catctagagg gccctattct atagtgtcac ctaaatgcta gagctcgctg atcagcctcg
     4021 actgtgcctt ctagttgcca gccatctgtt gtttgcccct cccccgtgcc ttccttgacc
     4081 ctggaaggtg ccactcccac tgtcctttcc taataaaatg aggaaattgc atcgcattgt
     4141 ctgagtaggt gtcattctat tctggggggt ggggtggggc aggacagcaa gggggaggat
     4201 tgggaagaca atagcaggca tgctggggat gcggtgggct ctatggcttc tgaggcggaa
     4261 agaaccagct ggggctctag ggggtatccc cacgcgccct gtagcggcgc attaagcgcg
     4321 gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct
     4381 cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta
     4441 aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa
     4501 cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct
     4561 ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc
     4621 aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg
     4681 ttaaaaaatg agctgattta acaaaaattt aacgcgaatt aattctgtgg aatgtgtgtc
     4741 agttagggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc
     4801 tcaattagtc agcaaccagg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc
     4861 aaagcatgca tctcaattag tcagcaacca tagtcccgcc cctaactccg cccatcccgc
     4921 ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt
     4981 atgcagaggc cgaggccgcc tctgcctctg agctattcca gaagtagtga ggaggctttt
     5041 ttggaggcct aggcttttgc aaaaagctcc ccgaaatgac cgaccaagcg acgcccaacc
     5101 tgccatcacg agatttcgat tccaccgccg ccttctatga aaggttgggc ttcggaatcg
     5161 ttttccggga cgccggctgg atgatcctcc agcgcgggga tctcatgctg gagttcttcg
     5221 cccaccccaa cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa
     5281 atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca
     5341 atgtatctta tcatgtctgt ataccgtcga cctctagcta gagcttggcg taatcatggt
     5401 catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg
     5461 gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt
     5521 tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg
     5581 gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg
     5641 actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa
     5701 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc
     5761 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc
     5821 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat
     5881 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc
     5941 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct
     6001 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg
     6061 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc
     6121 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga
     6181 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa
     6241 gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta
     6301 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc
     6361 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg
     6421 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga
     6481 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg
     6541 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct
     6601 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg
     6661 agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc
     6721 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa
     6781 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc
     6841 cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt
     6901 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc
     6961 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt
     7021 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc
     7081 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt
     7141 gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata
     7201 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga
     7261 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag
     7321 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa
     7381 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt
     7441 attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga
     7501 aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtcgacg
     7561 gatcgggaga tctcccgatc ccctatggtg cactctcagt acaatctgct ctgatgccgc
     7621 atagttaagc cagtatctgc tccctgcttg tgtgttggag gtcgctgagt agtgcgcgag
     7681 caaaatttaa gctacaacaa ggcaaggctt gaccgacaat tgcatgaaga atctgcttag
     7741 ggttaggcgt tttgcgctgc ttcgcgatgt acgggccaga tatacgcgtt
//
