LOCUS       sequence-443195-       24603 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Second generation Rosa26 gene targeting vector to induce a 
            Cre-dependent mosaic of cells expressing different chromatin 
            localized fluorescent proteins.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 24603)
  AUTHORS   Pontes-Quero S, Heredia L, Casquero-Garcia V, Fernandez-Chacon M, 
            Luo W, Hermoso A, Bansal M, Garcia-Gonzalez I, Sanchez-Munoz MS, 
            Perea JR, Galiana-Simal A, Rodriguez-Arabaolaza I, Del Olmo-Cabrera 
            S, Rocha SF, Criado-Rodriguez LM, Giovinazzo G, Benedito R
  TITLE     Dual ifgMosaic: A Versatile Method for Multispectral and 
            Combinatorial Mosaic Gene-Function Analysis.
  JOURNAL   Cell. 2017 Aug 10;170(4):800-814.e18. doi: 
            10.1016/j.cell.2017.07.031.
  PUBMED    28802047
REFERENCE   2  (bases 1 to 24603)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2017.07"; journalName: "Cell"; date: "2017-08-10- 
            10"; volume: "170"; issue: "4"; pages: "800-814.e18"
COMMENT     Sequence Label: iChr2-Control-Mosaic (SO274)
FEATURES             Location/Qualifiers
     source          1..24603
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        24..128
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(129..197,198..989)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(347..366)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1160..1748
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1649..1668
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1902..1919
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2036..2057
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2072..2077,2078..2095,2096..2102)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2110..2126
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2115..2137
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2134..2150
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2134..2150
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     2169..2189
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        2171..2189
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     2219..2235
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    join(5772..5837,5838..5875)
                     /label=Ad SA
                     /note="adenovirus major late transcript splice acceptor 
                     (Friedrich and Soriano, 1991)"
     enhancer        5904..6283
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        6285..6564
                     /label=chicken beta-actin promoter
     intron          6565..7581
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     7505..7527
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     7589..7608
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     protein_bind    complement(7642..7675)
                     /label=loxN
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AGGTATAC) (Shaw et al., 2021). loxN sites are 
                     compatible with each other, but incompatible with loxP or 
                     lox2272 sites."
     protein_bind    complement(7683..7716)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     protein_bind    complement(7725..7758)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     regulatory      7772..7781
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             7817..7837
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             7838..8542
                     /codon_start=1
                     /product="Phialidium yellow fluorescent proteim"
                     /label=PhiYFP
                     /note="mammalian codon-optimized"
                     /translation="MSSGALLFHGKIPYVVEMEGNVDGHTFSIRGKGYGDASVGKVDAQ
                     FICTTGDVPVPWSTLVTTLTAGAQCFAKYGPELKDFYKSCMPDGYVQERTITFEGDGNF
                     KTRAEVTFENGSVYNRVKLNGQGFKKDGHVLGKNLEFNFTPHCLYIWGDQANHGLKSAF
                     KICHEITGSKGDFIVADHTQMNTPIGGGPVHVPEYHHMSYHVKLSKDVTDHRDNMSLKE
                     TVRAVDCRKTYL"
     polyA_signal    8725..8846
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     protein_bind    complement(8858..8891)
                     /label=loxN
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AGGTATAC) (Shaw et al., 2021). loxN sites are 
                     compatible with each other, but incompatible with loxP or 
                     lox2272 sites."
     regulatory      8898..8907
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             8910..8927
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             8928..9302
                     /codon_start=1
                     /gene="HIST1H2BJ"
                     /product="human histone H2B"
                     /label=H2B
                     /translation="PEPAKSAPAPKKGSKKAVTKAQKKGGKKRKRSRKESYSIYVYKVL
                     KQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLPG
                     ELAKHAVSEGTKAITKYTSAK"
     CDS             9324..10034
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(9469..9487)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     9737..9756
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     misc_feature    10048..10636
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             complement(10519..10530)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    10770..10891
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     protein_bind    complement(10906..10939)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     regulatory      10947..10956
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             10953..11333
                     /codon_start=1
                     /gene="HIST1H2BJ"
                     /product="human histone H2B"
                     /label=H2B
                     /translation="MAPEPAKSAPAPKKGSKKAVTKAQKKGGKKRKRSRKESYSIYVYK
                     VLKQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLL
                     PGELAKHAVSEGTKAITKYTSAK"
     regulatory      11352..11361
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(11358..11360,11361..11363,11364..12077)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(11358..11360,11361..11363,11364..12074)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     misc_feature    12103..12691
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             complement(12574..12585)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    12825..12946
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     protein_bind    complement(12961..12994)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     regulatory      13001..13010
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             13013..13039
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             13040..13414
                     /codon_start=1
                     /gene="HIST1H2BJ"
                     /product="human histone H2B"
                     /label=H2B
                     /translation="PEPAKSAPAPKKGSKKAVTKAQKKGGKKRKRSRKESYSIYVYKVL
                     KQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLPG
                     ELAKHAVSEGTKAITKYTSAK"
     regulatory      13433..13442
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(13439..13441,13442..13444,13445..14158)
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Rizzo et al., 
                     2004)"
                     /label=mCerulean
                     /note=" mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNAISDNVYITADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     misc_feature    14194..14782
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             complement(14665..14676)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    14916..15037
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     protein_bind    15075..15108
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     promoter        15172..15671
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(15207..15230)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     15604..15623
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     promoter        15683..15730
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             15749..16552
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQ
                     GRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD
                     LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQ
                     DIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(15812..15831)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     16422..16441
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(16584..16601)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    16590..16814
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     protein_bind    16872..16905
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     misc_feature    19519..23860
                     /label=Rosa26 right arm
                     /note="right homology arm from intron 1 of the mouse Rosa26
                     gene (Soriano, 1999)"
     primer_bind     complement(23911..23927)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(23911..23927)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(23959..23978)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(23960..23978)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(23988..24005)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(23988..24004)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(23997..24019)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      complement(24145..24600)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(24282..24303)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     24494..24513
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
       61 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
      121 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt
      181 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt
      241 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt
      301 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg
      361 tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga
      421 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa
      481 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga
      541 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa
      601 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca
      661 ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta
      721 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac
      781 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc
      841 gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag
      901 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga
      961 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt
     1021 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
     1081 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
     1141 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
     1201 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
     1261 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc
     1321 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
     1381 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
     1441 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
     1501 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
     1561 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
     1621 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
     1681 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
     1741 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
     1801 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
     1861 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg
     1921 aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat
     1981 gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg
     2041 tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt
     2101 tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg
     2161 ccaagcgcgc aattaaccct cactaaaggg aacaaaagct gggtaccggg ccccccctcg
     2221 aggtcgacgg tatcgataag cttcacgtgt tcgaaacgcg gtccgccctg cagcaaccgg
     2281 agggggaggg agaagggagc ggaaaagtct ccaccggacg cggccatggc tcgggggggg
     2341 ggggggggca gcggaggagc gcttccggcc gacgtctcgt cgctgattgg cttcttttcc
     2401 tcccgccgtg tgtgaaaaca caaatggcgt gttttggttg gcgtaaggcg cctgtcagtt
     2461 aacggcagcc ggagtgcgca gccgccggca gcctcgctct gcccactggg tggggcggga
     2521 ggtaggtggg gtgaggcgag ctggacgtgc gggcgcggtc ggcctctggc ggggcggggg
     2581 aggggaggga gggtcagcga aagtagctcg cgcgcgagcg gccgcccacc ctccccttcc
     2641 tctgggggag tcgttttacc cgccgccggc cgggcctcgt cgtctgattg gctctcgggg
     2701 cccagaaaac tggcccttgc cattggctcg tgttcgtgca agttgagtcc atccgccggc
     2761 cagcgggggc ggcgaggagg cgctcccagg ttccggccct cccctcggcc ccgcgccgca
     2821 gagtctggcc gcgcgcccct gcgcaacgtg gcaggaagcg cgcgctgggg gcggggacgg
     2881 gcagtagggc tgagcggctg cggggcgggt gcaagcacgt ttccgacttg agttgcctca
     2941 agaggggcgt gctgagccag acctccatcg cgcactccgg ggagtggagg gaaggagcga
     3001 gggctcagtt gggctgtttt ggaggcagga agcacttgct ctcccaaagt cgctctgagt
     3061 tgttatcagt aagggagctg cagtggagta ggcggggaga aggccgcacc cttctccgga
     3121 ggggggaggg gagtgttgca atacctttct gggagttctc tgctgcctcc tggcttctga
     3181 ggaccgccct gggcacatga tggacctgtc gacaattcga gctcgctaga gccccatcct
     3241 cactgactcc gtcctggagt tggatgagag ataatggcct tacgttgtgc caggggaggg
     3301 tcgggctgga tttagcaaga tttaccttct ccaaagagcg gtgctgcagt ggcacagctg
     3361 cccacggagg tgggggggtc accgtccctg gaggtgatga agaactgtgg ggatgtggca
     3421 ctgagggaca tggccagtgg gcacggtggg tgggttgggg ttggtcttgg ggatcttgga
     3481 gggcttttcc agccttcatg atttgacgat tgtatgaaca tctacatggc aattctccag
     3541 ctgcctgtcc cagtcctact gacccagctg tatctctcca ggcaagctct tccacccctt
     3601 ctgcttgcat ccagacacca tcaaacatgc aggctcagac acagggacca gcagtgtctg
     3661 tggccttttt gtgctcctct ccatgctggg ttttaacttg ctctttgtcc ttctatccta
     3721 tcttcttatc cttaaggctg ttctgaacgc tgtgacttgg agagtgtccc agagccctca
     3781 acacctgcat gtcccacgtc catgctgtcc tgcacttcct tatccccaag atctgcctct
     3841 ccgtgatgca ctgaattggc aaacatgtgt caccccagac caacaatgtc acagcaaact
     3901 cccccttgat aggacaaggg ggaatggctt tacactgaga caggggaggt ttgggttgga
     3961 tatgaggagg cagtttttcc cccagagggt ggtgacgcac tgaacaggtt gcccaaggag
     4021 gctgtggatg ccccatccct gcaggcattc aaggccaggc tggatgtggc tctgggcagc
     4081 ctgggctgct ggttgatgac cctgcacata gcagggggtt ggatctggat gagcactgtg
     4141 ctcctttgca acccaggccg ttctatgatt ctgtcattct aaatctctct ttcagcctaa
     4201 agctttttcc ccgtatcccc ccaggtgtct gcaggctcaa agagcagcga gaagcgttca
     4261 gaggaaagcg atcccgtgcc accttccccg tgcccgggct gtccccgcac gctgccggct
     4321 cggggatgcg gggggagcgc cggaccggag cggagccccg ggcggctcgc tgctgccccc
     4381 tagcggggga gggacgtaat tacatccctg ggggctttgg gggggggctg tccctctagc
     4441 gagctcggta cccagagccc catcctcact gactccgtcc tggagttgga tgagagataa
     4501 tggccttacg ttgtgccagg ggagggtcgg gctggattta gcaagattta ccttctccaa
     4561 agagcggtgc tgcagtggca cagctgccca cggaggtggg ggggtcaccg tccctggagg
     4621 tgatgaagaa ctgtggggat gtggcactga gggacatggc cagtgggcac ggtgggtggg
     4681 ttggggttgg tcttggggat cttggagggc ttttccagcc ttcatgattt gacgattgta
     4741 tgaacatcta catggcaatt ctccagctgc ctgtcccagt cctactgacc cagctgtatc
     4801 tctccaggca agctcttcca ccccttctgc ttgcatccag acaccatcaa acatgcaggc
     4861 tcagacacag ggaccagcag tgtctgtggc ctttttgtgc tcctctccat gctgggtttt
     4921 aacttgctct ttgtccttct atcctatctt cttatcctta aggctgttct gaacgctgtg
     4981 acttggagag tgtcccagag ccctcaacac ctgcatgtcc cacgtccatg ctgtcctgca
     5041 cttccttatc cccaagatct gcctctccgt gatgcactga attggcaaac atgtgtcacc
     5101 ccagaccaac aatgtcacag caaactcccc cttgatagga caagggggaa tggctttaca
     5161 ctgagacagg ggaggtttgg gttggatatg aggaggcagt ttttccccca gagggtggtg
     5221 acgcactgaa caggttgccc aaggaggctg tggatgcccc atccctgcag gcattcaagg
     5281 ccaggctgga tgtggctctg ggcagcctgg gctgctggtt gatgaccctg cacatagcag
     5341 ggggttggat ctggatgagc actgtgctcc tttgcaaccc aggccgttct atgattctgt
     5401 cattctaaat ctctctttca gcctaaagct ttttccccgt atccccccag gtgtctgcag
     5461 gctcaaagag cagcgagaag cgttcagagg aaagcgatcc cgtgccacct tccccgtgcc
     5521 cgggctgtcc ccgcacgctg ccggctcggg gatgcggggg gagcgccgga ccggagcgga
     5581 gccccgggcg gctcgctgct gccccctagc gggggaggga cgtaattaca tccctggggg
     5641 ctttgggggg gggctgtccc tctaggggta cccggggatc ttaattggcc gtggctagtc
     5701 gacccagggc ctgggagaat cccttccccc tcttccctcg tgatctgcaa ctccagtctt
     5761 tctagcatct gtagggcgca gtagtccagg gtttccttga tgatgtcata cttatcctgt
     5821 cccttttttt tccacagctc gcggttgagg acaaactctt cgcggtcttt ccagtggtta
     5881 atgcaccttc cttccttcga gtcgacattg attattgact agttattaat agtaatcaat
     5941 tacggggtca ttagttcata gcccatatat ggagttccgc gttacataac ttacggtaaa
     6001 tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt
     6061 tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta
     6121 aactgcccac ttggcagtac atcaagtgta tcatatgcca agtacgcccc ctattgacgt
     6181 caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttat gggactttcc
     6241 tacttggcag tacatctacg tattagtcat cgctattacc atggtcgagg tgagccccac
     6301 gttctgcttc actctcccca tctccccccc cctccccacc cccaattttg tatttattta
     6361 ttttttaatt attttgtgca gcgatggggg cggggggggg ggggggggcg cgcgccaggc
     6421 ggggcggggc ggggcgaggg gcggggcggg gcgaggcgga gaggtgcggc ggcagccaat
     6481 cagagcggcg cgctccgaaa gtttcctttt atggcgaggc ggcggcggcg gcggccctat
     6541 aaaaagcgaa gcgcgcggcg ggcgggagtc gctgcgcgct gccttcgccc cgtgccccgc
     6601 tccgccgccg cctcgcgccg cccgccccgg ctctgactga ccgcgttact cccacaggtg
     6661 agcgggcggg acggcccttc tcctccgggc tgtaattagc gcttggttta atgacggctt
     6721 gtttcttttc tgtggctgcg tgaaagcctt gaggggctcc gggagggccc tttgtgcggg
     6781 gggagcggct cggggggtgc gtgcgtgtgt gtgtgcgtgg ggagcgccgc gtgcggctcc
     6841 gcgctgcccg gcggctgtga gcgctgcggg cgcggcgcgg ggctttgtgc gctccgcagt
     6901 gtgcgcgagg ggagcgcggc cgggggcggt gccccgcggt gcgggggggg ctgcgagggg
     6961 aacaaaggct gcgtgcgggg tgtgtgcgtg ggggggtgag cagggggtgt gggcgcgtcg
     7021 gtcgggctgc aaccccccct gcacccccct ccccgagttg ctgagcacgg cccggcttcg
     7081 ggtgcggggc tccgtacggg gcgtggcgcg gggctcgccg tgccgggcgg ggggtggcgg
     7141 caggtggggg tgccgggcgg ggcggggccg cctcgggccg gggagggctc gggggagggg
     7201 cgcggcggcc cccggagcgc cggcggctgt cgaggcgcgg cgagccgcag ccattgcctt
     7261 ttatggtaat cgtgcgagag ggcgcaggga cttcctttgt cccaaatctg tgcggagccg
     7321 aaatctggga ggcgccgccg caccccctct agcgggcgcg gggcgaagcg gtgcggcgcc
     7381 ggcaggaagg aaatgggcgg ggagggcctt cgtgcgtcgc cgcgccgccg tccccttctc
     7441 cctctccagc ctcggggctg tccgcggggg gacggctgcc ttcggggggg acggggcagg
     7501 gcggggttcg gcttctggcg tgtgaccggc ggctctagag cctctgctaa ccatgttcat
     7561 gccttcttct ttttcctaca gctcctgggc aacgtgctgg ttattgtgct gtctcatcat
     7621 tttggcaaag aattcctcga gataacttcg tatagtatac cttatacgaa gttatcgagc
     7681 tcataacttc gtataggata ctttatacga agttataact cgagataact tcgtatagca
     7741 tacattatac gaagttatac cggcagatct cgccaccatg gtgagtgtga ttacagaggc
     7801 tagcttcacc atggctccta agaagaagag gaaggtgatg agcagcggcg ccctgctgtt
     7861 ccacggcaag atcccctacg tggtggagat ggagggcaat gtggatggcc acaccttcag
     7921 catccgcggc aagggctacg gcgatgccag cgtgggcaag gtggatgccc agttcatctg
     7981 caccacaggc gatgtgcccg tgccctggag caccctggtg accaccctga ccgcaggcgc
     8041 ccagtgcttc gccaagtacg gccccgagct gaaggatttc tacaagagct gcatgcccga
     8101 tggctacgtg caggagcgca ccatcacctt cgagggtgat ggcaatttca agacccgcgc
     8161 cgaggtgacc ttcgagaatg gcagcgtgta caatcgcgtg aagctgaatg gccagggctt
     8221 caagaaggat ggccacgtgc tgggcaagaa tctggagttc aatttcaccc cccactgcct
     8281 gtacatctgg ggcgatcagg ccaatcacgg cctgaagagc gccttcaaga tctgccacga
     8341 gatcaccggc agcaagggcg atttcatcgt ggccgatcac acccagatga atacccccat
     8401 cggcggcggc cccgtgcacg tgcccgagta ccaccacatg agctaccacg tgaagctgag
     8461 caaggatgtg accgatcacc gcgataatat gagcctgaag gagaccgtgc gcgccgtgga
     8521 ttgccgcaag acctacctgt gacgcttaat tagctgagct tggactcctg ttgatagatc
     8581 cagtaatgac ctcagaactc catctggccg cgactctaga tcataatcag ccataccaca
     8641 tttgtagagg ttttacttgc tttaaaaaac ctcccacacc tccccctgaa cctgaaacat
     8701 aaaatgaatg caattgttgt tgttaacttg tttattgcag cttataatgg ttacaaataa
     8761 agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt
     8821 ttgtccaaac tcatcaatgt atcttaaggc gaagcttata acttcgtata gtatacctta
     8881 tacgaagtta tcggcgcgcc accatggcgc atcatcatca tcatcatcca gagccagcga
     8941 agtctgctcc cgccccgaaa aagggctcca agaaggcggt gactaaggcg cagaagaaag
     9001 gcggcaagaa gcgcaagcgc agccgcaagg agagctattc catctatgtc tacaaggttc
     9061 tgaagcaggt ccaccctgac accggcattt cgtccaaggc catgggcatc atgaattcgt
     9121 ttgtgaacga cattttcgag cgcatcgcag gtgaggcttc ccgcctggcg cattacaaca
     9181 agcgctcgac catcacctcc agggagatcc agacggccgt gcgcctgctg ctgcctgggg
     9241 agttggccaa gcacgccgtg tccgagggta ctaaggccat caccaagtac accagcgcta
     9301 aggatccccg ggtaccggta accatggtga gcaagggcga ggaggataac atggccatca
     9361 tcaaggagtt catgcgcttc aaggtgcaca tggagggctc cgtgaacggc cacgagttcg
     9421 agatcgaggg cgagggcgag ggccgcccct acgagggcac ccagaccgcc aagctgaagg
     9481 tgaccaaggg tggccccctg cccttcgcct gggacatcct gtcccctcag ttcatgtacg
     9541 gctccaaggc ctacgtgaag caccccgccg acatccccga ctacttgaag ctgtccttcc
     9601 ccgagggctt caagtgggag cgcgtgatga acttcgagga cggcggcgtg gtgaccgtga
     9661 cccaggactc ctccctgcag gacggcgagt tcatctacaa ggtgaagctg cgcggcacca
     9721 acttcccctc cgacggcccc gtaatgcaga agaagaccat gggctgggag gcctcctccg
     9781 agagaatgta ccccgaggac ggcgccctga agggcgagat caagcagagg ctgaagctga
     9841 aggacggcgg ccactacgac gctgaggtca agaccaccta caaggccaag aagcccgtgc
     9901 agctgcccgg cgcctacaac gtcaacatca agttggacat cacctcccac aacgaggact
     9961 acaccatcgt ggaacagtac gaacgcgccg agggccgcca ctccactggc ggcatggacg
    10021 agctgtacaa gtgaggcgcg ccccgctaat caacctctgg attacaaaat ttgtgaaaga
    10081 ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg
    10141 cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc
    10201 tggttgctgt ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc
    10261 actgtgtttg ctgacgcaac ccccactggt tggggcattg ccaccacctg tcagctcctt
    10321 tccgggactt tcgctttccc cctccctatt gccacggcgg aactcatcgc cgcctgcctt
    10381 gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt gttgtcgggg
    10441 aaatcatcgt cctttccttg gctgctcgcc tgtgttgcca cctggattct gcgcgggacg
    10501 tccttctgct acgtcccttc ggccctcaat ccagcggacc ttccttcccg cggcctgctg
    10561 ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg gatctccctt
    10621 tgggccgcct ccccgcatcg ataccgtcga gactgctgca ccagatcata atcagccata
    10681 ccacatttgt agaggtttta cttgctttaa aaaacctccc acacctcccc ctgaacctga
    10741 aacataaaat gaatgcaatt gttgttgtta acttgtttat tgcagcttat aatggttaca
    10801 aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt
    10861 gtggtttgtc caaactcatc aatgtatctt aaggcgtgga gatctataac ttcgtatagg
    10921 atactttata cgaagttatc ggtaccgcca ccatggcgcc agagccagcg aagtctgctc
    10981 ccgccccgaa aaagggctcc aagaaggcgg tgactaaggc gcagaagaaa ggcggcaaga
    11041 agcgcaagcg cagccgcaag gagagctatt ccatctatgt ctacaaggtt ctgaagcagg
    11101 tccaccctga caccggcatt tcgtccaagg ccatgggcat catgaactcg tttgtgaacg
    11161 acattttcga gcgcatcgca ggtgaggctt cccgcctggc gcattacaac aagcgctcga
    11221 ccatcacctc cagggagatc cagacggccg tgcgcctgct gctgcctggg gagttggcca
    11281 agcacgccgt gtccgagggt actaaggcca tcaccaagta caccagcgct aaggatcccc
    11341 gggtaccggt cgccaccatg gtgagcaagg gcgaggagct gttcaccggg gtggtgccca
    11401 tcctggtcga gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg
    11461 agggcgatgc cacctacggc aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc
    11521 ccgtgccctg gcccaccctc gtgaccaccc tgacctacgg cgtgcagtgc ttcagccgct
    11581 accccgacca catgaagcag cacgacttct tcaagtccgc catgcccgaa ggctacgtcc
    11641 aggagcgcac catcttcttc aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt
    11701 tcgagggcga caccctggtg aaccgcatcg agctgaaggg catcgacttc aaggaggacg
    11761 gcaacatcct ggggcacaag ctggagtaca actacaacag ccacaacgtc tatatcatgg
    11821 ccgacaagca gaagaacggc atcaaggtga acttcaagat ccgccacaac atcgaggacg
    11881 gcagcgtgca gctcgccgac cactaccagc agaacacccc catcggcgac ggccccgtgc
    11941 tgctgcccga caaccactac ctgagcaccc agtccgccct gagcaaagac cccaacgaga
    12001 agcgcgatca catggtcctg ctggagttcg tgaccgccgc cgggatcact ctcggcatgg
    12061 acgagctgta caagtgacct tccttaaccg gcgcgccccg ctaatcaacc tctggattac
    12121 aaaatttgtg aaagattgac tggtattctt aactatgttg ctccttttac gctatgtgga
    12181 tacgctgctt taatgccttt gtatcatgct attgcttccc gtatggcttt cattttctcc
    12241 tccttgtata aatcctggtt gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa
    12301 cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca ctggttgggg cattgccacc
    12361 acctgtcagc tcctttccgg gactttcgct ttccccctcc ctattgccac ggcggaactc
    12421 atcgccgcct gccttgcccg ctgctggaca ggggctcggc tgttgggcac tgacaattcc
    12481 gtggtgttgt cggggaaatc atcgtccttt ccttggctgc tcgcctgtgt tgccacctgg
    12541 attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc ggaccttcct
    12601 tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg
    12661 agtcggatct ccctttgggc cgcctccccg catcgatacc gtcgagactg ctgcaccaga
    12721 tcataatcag ccataccaca tttgtagagg ttttacttgc tttaaaaaac ctcccacacc
    12781 tccccctgaa cctgaaacat aaaatgaatg caattgttgt tgttaacttg tttattgcag
    12841 cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt
    12901 cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttaaggc gtggactagt
    12961 ataacttcgt atagcataca ttatacgaag ttatcggcgc gccaccatgg cgtatccata
    13021 tgacgtccca gactatgccc cagagccagc gaagtctgct cccgccccga aaaagggctc
    13081 caagaaggcg gtgactaagg cgcagaagaa aggcggcaag aagcgcaagc gcagccgcaa
    13141 ggagagctat tccatctatg tctacaaggt tctgaagcag gtccaccctg acaccggcat
    13201 ttcgtccaag gccatgggca tcatgaactc gtttgtgaac gacattttcg agcgcatcgc
    13261 aggtgaggct tcccgcctgg cgcattacaa caagcgctcg accatcacct ccagggagat
    13321 ccagacggcc gtgcgcctgc tgctgcctgg ggagttggcc aagcacgccg tgtccgaggg
    13381 tactaaggcc atcaccaagt acaccagcgc taaggatccc cgggtaccgg tcgccaccat
    13441 ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg agctggacgg
    13501 cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg
    13561 caagctgacc ctgaagttca tctgcaccac cggcaagctg cccgtgccct ggcccaccct
    13621 cgtgaccacc ctgacctggg gcgtgcagtg cttcgcccgc taccccgacc acatgaagca
    13681 gcacgacttc ttcaagtccg ccatgcccga aggctacgtc caggagcgca ccatcttctt
    13741 caaggacgac ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg acaccctggt
    13801 gaaccgcatc gagctgaagg gcatcgactt caaggaggac ggcaacatcc tggggcacaa
    13861 gctggagtac aacgccatca gcgacaacgt ctatatcacc gccgacaagc agaagaacgg
    13921 catcaaggcc aacttcaaga tccgccacaa catcgaggac ggcagcgtgc agctcgccga
    13981 ccactaccag cagaacaccc ccatcggcga cggccccgtg ctgctgcccg acaaccacta
    14041 cctgagcacc cagtccaagc tgagcaaaga ccccaacgag aagcgcgatc acatggtcct
    14101 gctggagttc gtgaccgccg ccgggatcac tctcggcatg gacgagctgt acaagtgacc
    14161 ttccttaacc ctcgagtcta ggcgcgcccc gctaatcaac ctctggatta caaaatttgt
    14221 gaaagattga ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct
    14281 ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat
    14341 aaatcctggt tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg
    14401 gtgtgcactg tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag
    14461 ctcctttccg ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc
    14521 tgccttgccc gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg
    14581 tcggggaaat catcgtcctt tccttggctg ctcgcctgtg ttgccacctg gattctgcgc
    14641 gggacgtcct tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc
    14701 ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc gccctcagac gagtcggatc
    14761 tccctttggg ccgcctcccc gcatcgatac cgtcgagact gctgcaccag atcataatca
    14821 gccataccac atttgtagag gttttacttg ctttaaaaaa cctcccacac ctccccctga
    14881 acctgaaaca taaaatgaat gcaattgttg ttgttaactt gtttattgca gcttataatg
    14941 gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt
    15001 ctagttgtgg tttgtccaaa ctcatcaatg tatcttaagg cgtggcggcc gcagcatagg
    15061 gatccccgaa ttccgaagtt cctattctct agaaagtata ggaacttcag gtctgaagag
    15121 gagtttacgt ccagccaagc tagcttggct gcaggtcgtc gaaattctac cgggtagggg
    15181 aggcgctttt cccaaggcag tctggagcat gcgctttagc agccccgctg ggcacttggc
    15241 gctacacaag tggcctctgg cctcgcacac attccacatc caccggtagg cgccaaccgg
    15301 ctccgttctt tggtggcccc ttcgcgccac cttctactcc tcccctagtc aggaagttcc
    15361 cccccgcccc gcagctcgcg tcgtgcagga cgtgacaaat ggaagtagca cgtctcacta
    15421 gtctcgtgca gatggacagc accgctgagc aatggaagcg ggtaggcctt tggggcagcg
    15481 gccaatagca gctttgctcc ttcgctttct gggctcagag gctgggaagg ggtgggtccg
    15541 ggggcgggct caggggcggg ctcaggggcg gggcgggcgc ccgaaggtcc tccggaggcc
    15601 cggcattctg cacgcttcaa aagcgcacgt ctgccgcgct gttctcctct tcctcatctc
    15661 cgggcctttc gacctgcagc ctgttgacaa ttaatcatcg gcatagtata tcggcatagt
    15721 ataatacgac aaggtgagga actaaaccat gggatcggcc attgaacaag atggattgca
    15781 cgcaggttct ccggccgctt gggtggagag gctattcggc tatgactggg cacaacagac
    15841 aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg caggggcgcc cggttctttt
    15901 tgtcaagacc gacctgtccg gtgccctgaa tgaactgcag gacgaggcag cgcggctatc
    15961 gtggctggcc acgacgggcg ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg
    16021 aagggactgg ctgctattgg gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc
    16081 tcctgccgag aaagtatcca tcatggctga tgcaatgcgg cggctgcata cgcttgatcc
    16141 ggctacctgc ccattcgacc accaagcgaa acatcgcatc gagcgagcac gtactcggat
    16201 ggaagccggt cttgtcgatc aggatgatct ggacgaagag catcaggggc tcgcgccagc
    16261 cgaactgttc gccaggctca aggcgcgcat gcccgacggc gatgatctcg tcgtgaccca
    16321 tggcgatgcc tgcttgccga atatcatggt ggaaaatggc cgcttttctg gattcatcga
    16381 ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata gcgttggcta cccgtgatat
    16441 tgctgaagag cttggcggcg aatgggctga ccgcttcctc gtgctttacg gtatcgccgc
    16501 tcccgattcg cagcgcatcg ccttctatcg ccttcttgac gagttcttct gaggggatca
    16561 attctctaga gctcgctgat cagcctcgac tgtgccttct agttgccagc catctgttgt
    16621 ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc actcccactg tcctttccta
    16681 ataaaatgag gaaattgcat cgcattgtct gagtaggtgt cattctattc tggggggtgg
    16741 ggtggggcag gacagcaagg gggaggattg ggaagacaat agcaggcatg ctggggatgc
    16801 ggtgggctct atggcttctg aggcggaaag aaccagctgg ggctcgacta gagcttgcgg
    16861 aacccttcgt tgaagttcct attctctaga aagtatagga acttcagtcg acgtgctcca
    16921 aggagagttt gctggcaaga tcttcggggt cgatgctaac acagggaatg tgcttgccta
    16981 tgagaggcta gaccgggaga aagtctctga gtatcgtcga caattcgagc tcgctagagc
    17041 cccatcctca ctgactccgt cctggagttg gatgagagat aatggcctta cgttgtgcca
    17101 ggggagggtc gggctggatt tagcaagatt taccttctcc aaagagcggt gctgcagtgg
    17161 cacagctgcc cacggaggtg ggggggtcac cgtccctgga ggtgatgaag aactgtgggg
    17221 atgtggcact gagggacatg gccagtgggc acggtgggtg ggttggggtt ggtcttgggg
    17281 atcttggagg gcttttccag ccttcatgat ttgacgattg tatgaacatc tacatggcaa
    17341 ttctccagct gcctgtccca gtcctactga cccagctgta tctctccagg caagctcttc
    17401 caccccttct gcttgcatcc agacaccatc aaacatgcag gctcagacac agggaccagc
    17461 agtgtctgtg gcctttttgt gctcctctcc atgctgggtt ttaacttgct ctttgtcctt
    17521 ctatcctatc ttcttatcct taaggctgtt ctgaacgctg tgacttggag agtgtcccag
    17581 agccctcaac acctgcatgt cccacgtcca tgctgtcctg cacttcctta tccccaagat
    17641 ctgcctctcc gtgatgcact gaattggcaa acatgtgtca ccccagacca acaatgtcac
    17701 agcaaactcc cccttgatag gacaaggggg aatggcttta cactgagaca ggggaggttt
    17761 gggttggata tgaggaggca gtttttcccc cagagggtgg tgacgcactg aacaggttgc
    17821 ccaaggaggc tgtggatgcc ccatccctgc aggcattcaa ggccaggctg gatgtggctc
    17881 tgggcagcct gggctgctgg ttgatgaccc tgcacatagc agggggttgg atctggatga
    17941 gcactgtgct cctttgcaac ccaggccgtt ctatgattct gtcattctaa atctctcttt
    18001 cagcctaaag ctttttcccc gtatcccccc aggtgtctgc aggctcaaag agcagcgaga
    18061 agcgttcaga ggaaagcgat cccgtgccac cttccccgtg cccgggctgt ccccgcacgc
    18121 tgccggctcg gggatgcggg gggagcgccg gaccggagcg gagccccggg cggctcgctg
    18181 ctgcccccta gcgggggagg gacgtaatta catccctggg ggctttgggg gggggctgtc
    18241 cctctagcga gctcggtacc cagagcccca tcctcactga ctccgtcctg gagttggatg
    18301 agagataatg gccttacgtt gtgccagggg agggtcgggc tggatttagc aagatttacc
    18361 ttctccaaag agcggtgctg cagtggcaca gctgcccacg gaggtggggg ggtcaccgtc
    18421 cctggaggtg atgaagaact gtggggatgt ggcactgagg gacatggcca gtgggcacgg
    18481 tgggtgggtt ggggttggtc ttggggatct tggagggctt ttccagcctt catgatttga
    18541 cgattgtatg aacatctaca tggcaattct ccagctgcct gtcccagtcc tactgaccca
    18601 gctgtatctc tccaggcaag ctcttccacc ccttctgctt gcatccagac accatcaaac
    18661 atgcaggctc agacacaggg accagcagtg tctgtggcct ttttgtgctc ctctccatgc
    18721 tgggttttaa cttgctcttt gtccttctat cctatcttct tatccttaag gctgttctga
    18781 acgctgtgac ttggagagtg tcccagagcc ctcaacacct gcatgtccca cgtccatgct
    18841 gtcctgcact tccttatccc caagatctgc ctctccgtga tgcactgaat tggcaaacat
    18901 gtgtcacccc agaccaacaa tgtcacagca aactccccct tgataggaca agggggaatg
    18961 gctttacact gagacagggg aggtttgggt tggatatgag gaggcagttt ttcccccaga
    19021 gggtggtgac gcactgaaca ggttgcccaa ggaggctgtg gatgccccat ccctgcaggc
    19081 attcaaggcc aggctggatg tggctctggg cagcctgggc tgctggttga tgaccctgca
    19141 catagcaggg ggttggatct ggatgagcac tgtgctcctt tgcaacccag gccgttctat
    19201 gattctgtca ttctaaatct ctctttcagc ctaaagcttt ttccccgtat ccccccaggt
    19261 gtctgcaggc tcaaagagca gcgagaagcg ttcagaggaa agcgatcccg tgccaccttc
    19321 cccgtgcccg ggctgtcccc gcacgctgcc ggctcgggga tgcgggggga gcgccggacc
    19381 ggagcggagc cccgggcggc tcgctgctgc cccctagcgg gggagggacg taattacatc
    19441 cctgggggct ttgggggggg gctgtccctc taggggtacc cggggatctt aattaacggc
    19501 ggcggagtat cgaattcgct agaagatggg cgggagtctt ctgggcaggc ttaaaggcta
    19561 acctggtgtg tgggcgttgt cctgcagggg aattgaacag gtgtaaaatt ggagggacaa
    19621 gacttcccac agattttcgg ttttgtcggg aagtttttta ataggggcaa ataaggaaaa
    19681 tgggaggata ggtagtcatc tggggtttta tgcagcaaaa ctacaggtta ttattgcttg
    19741 tgatccgcct cggagtattt tccatcgagg tagattaaag acatgctcac ccgagtttta
    19801 tactctcctg cttgagatcc ttactacagt atgaaattac agtgtcgcga gttagactat
    19861 gtaagcagaa ttttaatcat ttttaaagag cccagtactt catatccatt tctcccgctc
    19921 cttctgcagc cttatcaaaa ggtattttag aacactcatt ttagccccat tttcatttat
    19981 tatactggct tatccaaccc ctagacagag cattggcatt ttccctttcc tgatcttaga
    20041 agtctgatga ctcatgaaac cagacagatt agttacatac accacaaatc gaggctgtag
    20101 ctggggcctc aacactgcag ttcttttata actccttagt acactttttg ttgatccttt
    20161 gccttgatcc ttaattttca gtgtctatca cctctcccgt caggtggtgt tccacatttg
    20221 ggcctattct cagtccaggg agttttacaa caatagatgt attgagaatc caacctaaag
    20281 cttaactttc cactcccatg aatgcctctc tcctttttct ccatttataa actgagctat
    20341 taaccattaa tggtttccag gtggatgtct cctcccccaa tattacctga tgtatcttac
    20401 atattgccag gctgatattt taagacatta aaaggtatat ttcattattg agccacatgg
    20461 tattgattac tgcttactaa aattttgtca ttgtacacat ctgtaaaagg tggttccttt
    20521 tggaatgcaa agttcaggtg tttgttgtct ttcctgacct aaggtcttgt gagcttgtat
    20581 tttttctatt taagcagtgc tttctcttgg actggcttga ctcatggcat tctacacgtt
    20641 attgctggtc taaatgtgat tttgccaagc ttcttcagga cctataattt tgcttgactt
    20701 gtagccaaac acaagtaaaa tgattaagca acaaatgtat ttgtgaagct tggtttttag
    20761 gttgttgtgt tgtgtgtgct tgtgctctat aataatacta tccaggggct ggagaggtgg
    20821 ctcggagttc aagagcacag actgctcttc cagaagtcct gagttcaatt cccagcaacc
    20881 acatggtggc tcacaaccat ctgtaatggg atctgatgcc ctcttctggt gtgtctgaag
    20941 accacaagtg tattcacatt aaataaataa atcctccttc ttcttctttt tttttttttt
    21001 aaagagaata ctgtctccag tagaatttac tgaagtaatg aaatactttg tgtttgttcc
    21061 aatatggtag ccaataatca aattactctt taagcactgg aaatgttacc aaggaactaa
    21121 tttttatttg aagtgtaact gtggacagag gagccataac tgcagacttg tgggatacag
    21181 aagaccaatg cagactttaa tgtcttttct cttacactaa gcaataaaga aataaaaatt
    21241 gaacttctag tatcctattt gtttaaactg ctagctttac ttaacttttg tgcttcatct
    21301 atacaaagct gaaagctaag tctgcagcca ttactaaaca tgaaagcaag taatgataat
    21361 tttggatttc aaaaatgtag ggccagagtt tagccagcca gtggtggtgc ttgcctttat
    21421 gcctttaatc ccagcactct ggaggcagag acaggcagat ctctgagttt gagcccagcc
    21481 tggtctacac atcaagttct atctaggata gccaggaata cacacagaaa ccctgttggg
    21541 gaggggggct ctgagatttc ataaaattat aattgaagca ttccctaatg agccactatg
    21601 gatgtggcta aatccgtcta cctttctgat gagatttggg tattattttt tctgtctctg
    21661 ctgttggttg ggtcttttga cactgtgggc tttctttaaa gcctccttcc tgccatgtgg
    21721 tctcttgttt gctactaact tcccatggct taaatggcat ggctttttgc cttctaaggg
    21781 cagctgctga gatttgcagc ctgatttcca gggtggggtt gggaaatctt tcaaacacta
    21841 aaattgtcct ttaatttttt ttttaaaaaa tgggttatat aataaacctc ataaaatagt
    21901 tatgaggagt gaggtggact aatattaaat gagtccctcc cctataaaag agctattaag
    21961 gctttttgtc ttatacttaa cttttttttt aaatgtggta tctttagaac caagggtctt
    22021 agagttttag tatacagaaa ctgttgcatc gcttaatcag attttctagt ttcaaatcca
    22081 gagaatccaa attcttcaca gccaaagtca aattaagaat ttctgacttt taatgttaat
    22141 ttgcttactg tgaatataaa aatgatagct tttcctgagg cagggtctca ctatgtatct
    22201 ctgcctgatc tgcaacaaga tatgtagact aaagttctgc ctgcttttgt ctcctgaata
    22261 ctaaggttaa aatgtagtaa tacttttgga acttgcaggt cagattcttt tataggggac
    22321 acactaaggg agcttgggtg atagttggta aaatgtgttt caagtgatga aaacttgaat
    22381 tattatcacc gcaacctact ttttaaaaaa aaaagccagg cctgttagag catgcttaag
    22441 ggatccctag gacttgctga gcacacaaga gtagttactt ggcaggctcc tggtgagagc
    22501 atatttcaaa aaacaaggca gacaaccaag aaactacagt taaggttacc tgtctttaaa
    22561 ccatctgcat atacacaggg atattaaaat attccaaata atatttcatt caagttttcc
    22621 cccatcaaat tgggacatgg atttctccgg tgaataggca gagttggaaa ctaaacaaat
    22681 gttggttttg tgatttgtga aattgttttc aagtgatagt taaagcccat gagatacaga
    22741 acaaagctgc tatttcgagg tctcttggtt tatactcaga agcacttctt tgggtttccc
    22801 tgcactatcc tgatcatgtg ctaggcctac cttaggctga ttgttgttca aataaactta
    22861 agtttcctgt caggtgatgt catatgattt catatatcaa ggcaaaacat gttatatatg
    22921 ttaaacattt gtacttaatg tgaaagttag gtctttgtgg gtttgatttt taattttcaa
    22981 aacctgagct aaataagtca tttttacatg tcttacattt ggtggaattg tataattgtg
    23041 gtttgcaggc aagactctct gacctagtaa ccctacctat agagcacttt gctgggtcac
    23101 aagtctagga gtcaagcatt tcaccttgaa gttgagacgt tttgttagtg tatactagtt
    23161 tatatgttgg aggacatgtt tatccagaag atattcagga ctatttttga ctgggctaag
    23221 gaattgattc tgattagcac tgttagtgag cattgagtgg cctttaggct tgaattggag
    23281 tcacttgtat atctcaaata atgctggcct tttttaaaaa gcccttgttc tttatcaccc
    23341 tgttttctac ataatttttg ttcaaagaaa tacttgtttg gatctccttt tgacaacaat
    23401 agcatgtttt caagccatat tttttttcct tttttttttt tttttttggt ttttcgagac
    23461 agggtttctc tgtatagccc tggctgtcct ggaactcact ttgtagacca ggctggcctc
    23521 gaactcagaa atccgcctgc ctctgcctcc tgagtgccgg gattaaaggc gtgcaccacc
    23581 acgcctggct aagttggata ttttgttata taactataac caatactaac tccactgggt
    23641 ggatttttaa ttcagtcagt agtcttaagt ggtctttatt ggcccttcat taaaatctac
    23701 tgttcactct aacagaggct gttggtacta gtggcactta agcaacttcc tacggatata
    23761 ctagcagatt aagggtcagg gatagaaact agtctagcgt tttgtatacc taccagcttt
    23821 atactacctt gttctgatag aaatatttca ggacatctag cttatcgata ccgtcgacgt
    23881 tcgaacacgt ggaattcctg cagcccgggg gatccactag ttctagagcg gccgccaccg
    23941 cggtggagct ccaattcgcc ctatagtgag tcgtattacg cgcgctcact ggccgtcgtt
    24001 ttacaacgtc gtgactggga aaaccctggc gttacccaac ttaatcgcct tgcagcacat
    24061 ccccctttcg ccagctggcg taatagcgaa gaggcccgca ccgatcgccc ttcccaacag
    24121 ttgcgcagcc tgaatggcga atggaaattg taagcgttaa tattttgtta aaattcgcgt
    24181 taaatttttg ttaaatcagc tcatttttta accaataggc cgaaatcggc aaaatccctt
    24241 ataaatcaaa agaatagacc gagatagggt tgagtgttgt tccagtttgg aacaagagtc
    24301 cactattaaa gaacgtggac tccaacgtca aagggcgaaa aaccgtctat cagggcgatg
    24361 gcccactacg tgaaccatca ccctaatcaa gttttttggg gtcgaggtgc cgtaaagcac
    24421 taaatcggaa ccctaaaggg agcccccgat ttagagcttg acggggaaag ccggcgaacg
    24481 tggcgagaaa ggaagggaag aaagcgaaag gagcgggcgc tagggcgctg gcaagtgtag
    24541 cggtcacgct gcgcgtaacc accacacccg ccgcgcttaa tgcgccgcta cagggcgcgt
    24601 cag
//
