LOCUS       sequence-443282-        7560 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express Pex5 and Pex11.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7560)
  AUTHORS   Baker JJ, Shi J, Wang S, Mujica EM, Bianco S, Capponi S, Dueber JE
  TITLE     ML-enhanced peroxisome capacity enables compartmentalization of 
            multienzyme pathway.
  JOURNAL   Nat Chem Biol. 2024 Oct 14. doi: 10.1038/s41589-024-01759-2.
  PUBMED    39402374
REFERENCE   2  (bases 1 to 7560)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Chem 
            Biol. 2024 Oct 14. doi: 10.1038/s41589-024-01759-2."
COMMENT     Sequence Label: pJJB1536
FEATURES             Location/Qualifiers
     source          1..7560
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        299..712
                     /gene="S. cerevisiae TEF1"
                     /label=TEF1 promoter
                     /note="promoter for EF-1-alpha"
     terminator      2586..2773
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(2641..2665)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     promoter        3327..3740
                     /gene="S. cerevisiae TEF1"
                     /label=TEF1 promoter
                     /note="promoter for EF-1-alpha"
     CDS             complement(5298..6113)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(5298..6113)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     6021..6040
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     6105..6124
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      6338..6926
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6827..6846
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 actggccgat aattgcagac gaacgccttg ccaacaggga gttcttcaga gacatggagg
       61 ctcaaaacga aattattgac agcctagaca tcaatagtca tacaacagaa agcgaccacc
      121 caactttggc tgataatagc gtataaacaa tgcatacttt gtacgttcaa aatacaatgc
      181 agtagatata tttatgcata ttacatataa tacatatcac ataggaagca acaggcgcgt
      241 tggactttta attttcgagg accgcgaatc cttacatcac acccaatccc ccacaagtga
      301 tcccccacac accatagctt caaaatgttt ctactccttt tttactcttc cagattttct
      361 cggactccgc gcatcgccgt accacttcaa aacacccaag cacagcatac taaatttccc
      421 ctctttcttc ctctagggtg tcgttaatta cccgtactaa aggtttggaa aagaaaaaag
      481 acaccgcctc gtttcttttt cttcgtcgaa aaaggcaata aaaattttta tcacgtttct
      541 ttttcttgaa aatttttttt tttgattttt ttctctttcg atgacctccc attgatattt
      601 aagttaataa acggtcatca atttctcaag tttcagtttc atttttcttg ttctattaca
      661 acttttttta cttcttgctc attagaaaga aagcatagca atctaatcta agttttaatt
      721 acaaaagatc tatggacgta ggaagttgct cagtgggaaa taatccgctt gcgcagttgc
      781 acaaacatac tcagcagaac aaatcgcttc agtttaatca gaagaataat gggcgtctta
      841 atgagtcacc tctacagggt accaacaagc caggtattag tgaggctttt atatccaatg
      901 ttaatgctat ttcacaagaa aacatggcga atatgcaaag gttcataaac ggagaaccac
      961 tgatcgatga taaaagaaga atggaaatag ggccatcctc aggcaggctt ccaccttttt
     1021 caaacgtaca ttctctacag acttcagcaa acccaaccca aattaaggga gtgaacgata
     1081 tatctcattg gtcacaggaa tttcaaggta gtaatagtat tcaaaataga aacgcggata
     1141 caggaaattc agaaaaggca tggcagcgtg gctcaacaac cgcatcaagc cggtttcagt
     1201 accctaatac catgatgaat aactatgctt atgcttctat gaacagtctt agtggatcaa
     1261 ggctccaatc gcctgctttc atgaatcaac aacagtctgg tcgttctaaa gaaggagtca
     1321 atgagcaaga acaacaaccc tggacagatc agtttgaaaa gctggaaaaa gaagtctcag
     1381 aaaacttgga cataaatgat gaaatagaga aggaggaaaa tgtgagtgaa gtagaacaaa
     1441 acaaaccaga aactgttgag aaggaagaag gagtatatgg agatcagtat caatctgatt
     1501 tccaagaagt gtgggatagc atacacaagg acgctgaaga agtcttgcca tccgaattag
     1561 ttaatgatga cctcaatcta ggagaagact acttgaaata tctcggcggt agagtaaatg
     1621 ggaacatcga gtatgctttt caatctaaca acgaatattt taataatcct aatgcttata
     1681 aaattggctg cctactgatg gaaaacggag ccaaattgag cgaggcagcg ctagcatttg
     1741 aagctgctgt taaagaaaaa ccggaccatg tggatgcatg gctaagattg ggtctagtac
     1801 aaacccagaa tgaaaaagag ttgaacggca taagcgccct cgaagaatgt ttaaagttag
     1861 acccaaagaa tctggaggca atgaaaactt tagcgataag ttatataaac gaaggttatg
     1921 atatgagcgc cttcacaatg ctggataaat gggcagaaac taagtacccg gaaatttggt
     1981 caaggatcaa gcaacaagat gacaaatttc aaaaagagaa agggtttacc catattgata
     2041 tgaacgctca tatcacaaag caatttttgc aactagcaaa caatttaagc acaatagatc
     2101 ctgaaataca actatgcttg ggactcttat tttacacgaa agatgatttt gacaaaacca
     2161 tagattgctt tgaaagtgcg ttgagggtga atcctaatga cgaactcatg tggaatagat
     2221 taggggcttc attggccaat tccaatagat cagaggaagc aatccaagcc tatcataggg
     2281 cactacaact aaaaccttct tttgttagag ctcgctataa tctggcggta tcatccatga
     2341 atataggctg tttcaaagaa gcagcaggct acttattaag tgttctaagt atgcatgaag
     2401 tgaacactaa taataaaaaa ggagatgttg gatctctctt gaatacgtac aatgatactg
     2461 ttatagagac tttgaagaga gtttttatag cgatgaatag agatgattta cttcaagaag
     2521 tgaagccagg catggacctg aaaagattta aaggagaatt ttcgttttga ggatcctaac
     2581 tcgaggcgaa tttcttatga tttatgattt ttattattaa ataagttata aaaaaaataa
     2641 gtgtatacaa attttaaagt gactcttagg ttttaaaacg aaaattctta ttcttgagta
     2701 actctttcct gtaggtcagg ttgctttctc aggtatagca tgaggtcgct cttattgacc
     2761 acacctctac cggcatgccg agcaaatgcc tgcaaatcgc tccccatttc gctgccaaac
     2821 cagatgtcaa cacagctaca acgactagag atctatgtga ggatcctaac tcgagatcga
     2881 gctggatgca cacactggct taagatgaca acgactagag atctatgtga ggatcctaac
     2941 tcgagatcga gctggttctg tatgggcaca gacaacctaa acgactagag atctatgtga
     3001 ggatcctaac tcgagatcga gctgggtaga ctacccatga gtcacaatga acgccttgcc
     3061 aacagggagt tcttcagaga catggaggct caaaacgaaa ttattgacag cctagacatc
     3121 aatagtcata caacagaaag cgaccaccca actttggctg ataatagcgt ataaacaatg
     3181 catactttgt acgttcaaaa tacaatgcag tagatatatt tatgcatatt acatataata
     3241 catatcacat aggaagcaac aggcgcgttg gacttttaat tttcgaggac cgcgaatcct
     3301 tacatcacac ccaatccccc acaagtgatc ccccacacac catagcttca aaatgtttct
     3361 actccttttt tactcttcca gattttctcg gactccgcgc atcgccgtac cacttcaaaa
     3421 cacccaagca cagcatacta aatttcccct ctttcttcct ctagggtgtc gttaattacc
     3481 cgtactaaag gtttggaaaa gaaaaaagac accgcctcgt ttctttttct tcgtcgaaaa
     3541 aggcaataaa aatttttatc acgtttcttt ttcttgaaaa tttttttttt tgattttttt
     3601 ctctttcgat gacctcccat tgatatttaa gttaataaac ggtcatcaat ttctcaagtt
     3661 tcagtttcat ttttcttgtt ctattacaac tttttttact tcttgctcat tagaaagaaa
     3721 gcatagcaat ctaatctaag ttttaattac aaaagatcta tggtctgtga tacactggta
     3781 tatcatccct ccgtgacgag attcgtcaaa tttctagatg gctcagctgg cagagaaaag
     3841 gttctcagat tactgcagta tttagcaaga tttttagcag tacagaactc atctctcttg
     3901 gccaggcaat tacaagctca atttaccaca gtcagaaaat ttctgaggtt tctgaagcct
     3961 ttaaaccact tgcaggcagc tgctaaattc tacgataaca agttggccag cgacaacgtc
     4021 gttagagtct gcaatgtttt gaaaaacatc ttctttgctg catatttgtc gttagatcaa
     4081 gtcaatcttt tgagaatttt gaaagtgatt cctgtaaccg ttcttaccgg taagaaaata
     4141 cctcgctggt ccaattggtg ttggctgttc ggcctcctaa gtggtctggc tatggatctt
     4201 cgtaagatcc aaacatctca tgcccagatt gctgcgtttg tcaaggcaaa atcacaaagc
     4261 caaggcgatg agcatgagga tcacaagaag gtactaggga aggcatacca agacaggtac
     4321 accgcgttaa gaagactatt ctgggatgct gcagattcgt tcatcgtcct caacaaccta
     4381 gggtacttgt ctagcaacga agagtatgtt gcgctatccg gtgttgtcac atctatcctt
     4441 ggtatgcaag acatgtggaa agctacatag ggatcctaac tcgagattga attgaattga
     4501 aatcgataga tcaatttttt tcttttctct ttccccatcc tttacgctaa aataatagtt
     4561 tattttattt tttgaatatt ttttatttat atacgtatat atagactatt atttatcttt
     4621 taatgattat taagattttt attaaaaaaa aattcgctcc tcttttaatg cctttatgca
     4681 gttttttttt cccattcgat atttctatgt gctgagcagt tacagagatg ttacgaacca
     4741 ctagtgcact gcagtacatg acatgtatgg gttgaaaata tttagaggat gctaaaagtc
     4801 ccgtctaaaa tagaggaagt catggattac attatcacct ttattgtcct gttccgtctg
     4861 gctaatttgg gacgaggaac gtttgtctcc attcgcaacc ttttgaaacc atatgaacgg
     4921 cggctaatct gacagatgtc ttatatttct aggattgtag aaagcctcct catcaaacca
     4981 ccttgtttga aaaaggcact agtgcggttg ctttacttga tctagtattc tcctaccttg
     5041 gagcggtccc atatcaacta aattcagttt tctgactgag ttaaaaacta atgtagcgac
     5101 accacttcca ttggtgcctc cgttattttt tttttgtatg ttaatgctaa ataatacacc
     5161 gctatgtatt tcagggcact acttctacac atcaacggta ctaaacattt cgctcaagat
     5221 cggtccgctt tcacgtattt ggatgctatg caatgttgcc gagcggccgc gtgttacaac
     5281 caattaacca attctgatta gaaaaactca tcgagcatca aatgaaactg caatttattc
     5341 atatcaggat tatcaatacc atatttttga aaaagccgtt tctgtaatga aggagaaaac
     5401 tcaccgaggc agttccatag gatggcaaga tcctggtatc ggtctgcgat tccgactcgt
     5461 ccaacatcaa tacaacctat taatttcccc tcgtcaaaaa taaggttatc aagtgagaaa
     5521 tcaccatgag tgacgactga atccggtgag aatggcaaaa gcttatgcat ttctttccag
     5581 acttgttcaa caggccagcc attacgctcg tcatcaaaat cactcgcatc aaccaaaccg
     5641 ttattcattc gtgattgcgc ctgagcgagg cgaaatacgc gatcgctgtt aaaaggacaa
     5701 ttacaaacag gaatcgaatg caaccggcgc aggaacactg ccagcgcatc aacaatattt
     5761 tcacctgaat caggatattc ttctaatacc tggaatgctg ttttcccggg gatcgcagtg
     5821 gtgagtaacc atgcatcatc aggagtacgg ataaaatgct tgatggtcgg aagaggcata
     5881 aattccgtca gccagtttag tctgaccatc tcatctgtaa catcattggc aacgctacct
     5941 ttgccatgtt tcagaaacaa ctctggcgca tcgggcttcc catacaatcg atagattgtc
     6001 gcacctgatt gcccgacatt atcgcgagcc catttatacc catataaatc agcatccatg
     6061 ttggaattta atcgcggcct ggagcaagac gtttcccgtt gaatatggct cataacaccc
     6121 cttgtattac tgtttatgta agcagacagt tttattgttc atgatgatat atttttatct
     6181 tgtgcaatgt aacatcagag attttgagac acaacgtggc tttgttgaat aaatcgaact
     6241 tttgctgagt tgaaggatca gtcatgacca aaatccctta acgtgagttt tcgttccact
     6301 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
     6361 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
     6421 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
     6481 ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
     6541 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
     6601 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
     6661 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
     6721 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg
     6781 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
     6841 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct
     6901 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg
     6961 ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata
     7021 accgtgcggc cgccaattga aaaaggccac tactgagcca agcgcagaac cagatgagcc
     7081 attcattgga tacttgggtg acgttactgc atctataaac caagatataa aagaatatgg
     7141 tcgcagaaga acagtaatac ctacaagcga tttagtagcg tcgttctaca ccaaaaagac
     7201 gcctgttttt aaggtcaagt tcagcagaag caatttagcc cttgcaggag gtgctttcag
     7261 gccataattc acactgaaaa aaataacagc atggaagtga tatgtaataa gtataaccta
     7321 atccgatatg taactattag atgctttttc gacatcgtac ttgacctgct cggcggttat
     7381 catagcgtct tctttagcct tttttttttc gctcagtcat cggggccaaa acaagaaagg
     7441 cgaagaacag aaaaatgaaa aatgtaactg gttgttgtaa aaaccttcca gagaaataag
     7501 atgaaatgta tgttgctggc acgaacgaag acaaataccc tgaattcgca tctagactga
//
