LOCUS       sequence-443287-        8522 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8522)
  AUTHORS   Munger K, Phelps WC, Bubb V, Howley PM, Schlegel R
  TITLE     The E6 and E7 genes of the human papillomavirus type 16 together are
            necessary and sufficient for transformation of primary human 
            keratinocytes.
  JOURNAL   J Virol. 1989 Oct . 63(10):4417-21.
  PUBMED    2476573
REFERENCE   2  (bases 1 to 8522)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Virol. 
            1989 Oct . 63(10):4417-21."
COMMENT     Sequence Label: p1322 HPV-16 E6
FEATURES             Location/Qualifiers
     source          1..8522
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(11..28)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(182..770)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(262..281)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(941..1732,1733..1801))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1564..1583
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1802..1906)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     1974..1992
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
       61 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
      121 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
      181 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
      241 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
      301 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
      361 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
      421 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
      481 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
      541 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
      601 ctaactacgg ctacactaga aggacagtat ttggtatctg cgctctgctg aagccagtta
      661 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
      721 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
      781 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
      841 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta
      901 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg
      961 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg
     1021 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc
     1081 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg
     1141 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg
     1201 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctgcag
     1261 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat
     1321 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc
     1381 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc
     1441 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa
     1501 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaacac
     1561 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt
     1621 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc
     1681 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa
     1741 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca
     1801 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat
     1861 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa
     1921 aagtgccacc tgacgtctaa gaaaccatta ttatcatgac attaacctat aaaaataggc
     1981 gtatcacgag gccctttctt atcatcgaat tccgactttc aaacagcctt tggactcagg
     2041 ccttggggac attccagggg accctttacc agggaggggc agtgtgaggc aggaggcagg
     2101 gctgccgcct cagggaccct gagcctagag tccttcccac cgcatttggg gacttgagca
     2161 cttcaccagt cctgactcag tttcctagtc tgtgaatggg ggtgtgtctc ctggaatgtt
     2221 gccccccctc attcattcat tctcctctca ctgagcacct accgtgttca gggtccctgt
     2281 cctcctagag ctaactcaag tcttggaggg aagctctgtc tcagttcctt cctctacaaa
     2341 atggagcttg aggacgggca cggtggctca tgcctgtaat cccaggactt tgggaggctg
     2401 aggtgggagg atcgcttgag ccccccggag ctggagacca gcctgggcaa catggtgaga
     2461 agccatacct ctacagataa tttaaaaatt agccaggctt ggaagtgtgt gcctatagtc
     2521 ccagctactc gtgaaggctt tgagcccagg aggtcgaggc tgcagtgagc tgtgatcgca
     2581 ctccagcctg ggcatcacag caagaccttg tatcaaaaat agtaataata ataaaaagga
     2641 ggttggattc cctcctggca ggatagggag ggcgctgcag tgcccagggc aaggtggctg
     2701 ggtggttgtt ttgcgggagg gccaaggagt ggtccctggg tctgcgctgt aagagttggt
     2761 tgcctggagg ccttgcaggg tgggggtgtc accaaagcaa aggcttggag gggaaaaaac
     2821 aaaagtccca gccaggcagg tggagtcccc ttggctgagt tcaaccgagg gttctccggg
     2881 ggctgcgtgc gtgccccagt gacagctccg aaagctccct tacagggcaa agttcccaag
     2941 cacagaagag aacctgttca cttctcccct gctcggcccg ccccctggcc aggcacctct
     3001 acttcctctt ttcctgctcc gctgcttgct ttctctcttc agctcctccc tgcccctcac
     3061 cccaggctgc tcggccacct ccaacctgcc acctgaggac acccaggcag tcactcattc
     3121 aacagcgagg agccctgggg tgggtgtagt gggaaggagt gggggtgacg gagaccctgg
     3181 gagggctcgc agcctggtgg ctgaggccca gttctaaatg ccagctgcaa gccttggtct
     3241 gaggtaggga ggaaggcgtg gctgcagagg ctaaaacgct tccccaaaga ggggctttct
     3301 gggatgggac ttgaagggtg cataggagag cactaggaag tggccgctgc agacagaggg
     3361 aaccacaagc caggaggaca ggccaggaat gctgcagccc ggggcggggt ggggctggag
     3421 ctcctgtctc ttggccagct gaatggaggc ccagcggcaa cacaggtcct gcctggggat
     3481 caggtctgct ctgcacccca ccttgctgcc tggagccgcc cacctgacaa cctctcatcc
     3541 ctgctctgta gatccggtcc catccccact gcccacccca cccccccagc actccaccca
     3601 gttcaacgtt ccacgaaccc ccagaaccag ccctcatcaa caggcagcaa gaagggcccc
     3661 ccgcccatcg ccccacaacg ccagccgggt gaactgtagc gttggcaggt cctgaggcag
     3721 ctggcaagac gcctgcagct gaaagataca aggccaggga caggacagtc ccatccccag
     3781 gaggcaggga gtatacaggc tggggaagtt tgcccttgcg tggggtggtg atggaggagg
     3841 ctcagcaagt cttctggact gtgaacctgt gtctgccact gtgtgctggg tggtggtcat
     3901 ctttcccacc aggctgtggc ctctgcaacc ttcaagggag gagcaggtcc cattggctga
     3961 gcacagcctt gtaccgtgaa ctggaacaag cagcctcctt cctggccaca ggttccatgt
     4021 ccttatatgg actcatcttt gcctattgcg acacacactc aatgaacacc tactacgcgc
     4081 tgcaaagagc cccgcaggcc tgaggtgccc ccacctcacc actcttccta tttttgtgta
     4141 aaaatccagc ttcttgtcac cacctccaag gagggggagg aggaggaagg caggttcctc
     4201 taggctgagc cgaatgcccc tctgtggtcc cacgccactg atcgctgcat gcccaccacc
     4261 tgggtacaca cagtctgtga ttcccggagc agaacggacc ctgcccaccc ggtcttgtgt
     4321 gctactcagt ggacagaccc aaggcaagaa agggtgacaa ggacagggtc ttcccaggct
     4381 ggctttgagt tcctagcacc gccccgcccc caatcctctg tggcacatgg agtcttggtc
     4441 cccagagtcc cccagcggcc tccagatggt ctgggagggc agttcagctg tggctgcgca
     4501 tagcagacat acaacggacg gtgggcccag acccaggctg tgtagaccca gcccccccgc
     4561 cccgcagtgc ctaggtcacc cactaacgcc ccaggcctgg tcttggctgg gcgtgactgt
     4621 taccctcaaa agcaggcagc tccagggtaa aaggtgccct gccctgtaga gcccaccttc
     4681 cttcccaggg ctgcggctgg gtaggtttgt agccttcatc acgggccacc tccagccact
     4741 ggaccgctgg cccctgccct gtcctgggga gtgtggtcct gcgacttcta agtggccgca
     4801 agccacctga ctcccccaac accacactct acctctcaag cccaggtctc tccctagtga
     4861 cccacccagc acatttagct agctgagccc cacagccaga ggtcctcagg ccctgctttc
     4921 agggcagttg ctctgaagtc ggcaaggggg agtgactgcc tggccactcc atgccctcca
     4981 agagctcctt ctgcaggagc gtacagaacc cagggccctg gcacccgtgc agaccctggc
     5041 ccaccccacc tgggcgctca gtgcccaaga gatgtccaca cctaggatgt cccgcggtgg
     5101 gtggggggcc cgagagacgg gcaggccggg ggcaggcctg gccatgcggg gccgaaccgg
     5161 gcactgccca gcgtggggcg cgggggccac ggcgcgcgcc cccagccccc gggcccagca
     5221 ccccaaggcg gccaacgcca aaactctccc tcctcctctt cctcaatctc gctctcgctc
     5281 tttttttttt tcgcaaaagg aggggagagg gggtaaaaaa atgctgcact gtgcggcgaa
     5341 gccggtgagt gagcggcgcg gggccaatca gcgtgcgccg ttccgaaagt tgccttttat
     5401 ggctcgagcg gccgcggcgg cgccctataa aacccagcgg cgcgacgcgc caccaccgcc
     5461 gagaccgcgt ccgccccgcg agcacagagc ctcgcctttg ccgatccgcc gcccgtccac
     5521 acccgccgcc aggtaagccc ggccagccga ccggggcatg cggccgcggc cccttcgccc
     5581 gtgcagagcc gccgtctggg ccgcagcggg gggcgcatgg ggggggaacc ggaccgccgt
     5641 ggggggcgcg ggagaagccc ctgggcctcc ggagatgggg gacaccccac gccagttcgg
     5701 aggcgcgagg ccgcgctcgg gaggcgcgct ccgggggtgc cgctctcggg gcgggggcaa
     5761 ccggcggggt ctttgtctga gccgggctct tgccaatggg gatcgcaggg tgggcgcggc
     5821 gtagcccccg ccaggcccgg tgggggctgg ggcgccattg cgcgtgcgcg ctggtccttt
     5881 gggcgctaac tgcgtgcgcg ctgggaattg gcgctaattg cgcgtgcgcg ctgggactca
     5941 aggcgctaat tgcgcgtgcg ttctggggcc cggggtgccg cggcctgggc tggggcgaag
     6001 gcgggctcgg ccggaagggg tggggtcgcc gcggctcccg ggcgcttgcg cgcacttcct
     6061 gcccgagccg ctggccgccc gagggtgtgg ccgctgcgtg cgcgcgcgcc gacccggcgc
     6121 tgtttgaacc gggcggaggc ggggctggcg cccggttggg agggggttgg ggcctggctt
     6181 cctgccgcgc gccgcgggga cgcctccgac cagtgtttgc cttttatggt aataacgcgg
     6241 ccggcccggc ttcctttgtc cccaatctgg gcgcgcgccg gcgccccctg gcggcctaag
     6301 gactcggcgc gccggaagtg gccagggcgg gggcgacttc ggctcacagc gcgcccggct
     6361 attctcgcag gatcagtcga ctctagagga tccaccatgt ttcaggaccc acaggagcga
     6421 cccagaaagt taccacagtt atgcacagag ctgcaaacaa ctatacatga tataatatta
     6481 gaatgtgtgt actgcaagca acagttactg cgacgtgagg tatatgactt tgcttttcgg
     6541 gatttatgca tagtatatag agatgggaat ccatatgctg tatgtgataa atgtttaaag
     6601 ttttattcta aaattagtga gtatagacat tattgttata gtttgtatgg aacaacatta
     6661 gaacagcaat acaacaaacc gttgtgtgat ttgttaatta ggtgtattaa ctgtcaaaag
     6721 ccactgtgtc ctgaagaaaa gcaaagacat ctggacaaaa agcaaagatt ccataatata
     6781 aggggtcggt ggaccggtcg atgtatgtct tgttgcagat catcaagaac acgtagagaa
     6841 acccagctgt aaggtaccag atctgaattc aagcttggaa ttctcatgtt tgacagctta
     6901 tcatcgataa gctactcagg atttaaaaac tggaacggtg aaggtgacag cagtcggttg
     6961 gagcgagcat cccccaaagt tcacaatgtg gccgaggact ttgattgcac attgttgttt
     7021 ttttaatagt cattccaaat atgagatgca ttgttacagg aagtcccttg ccatcctaaa
     7081 agccacccca cttctctcta aggagaatgg cccagtcctc tcccaagtcc acacagggga
     7141 ggtgatagca ttgctttcgt gtaaattatg taatgcaaaa tttttttaat cttcgcctta
     7201 atactttttt attttgtttt attttgaatg atgagccttc gtgccccccc ttcccccttt
     7261 tttgtccccc aacttgagat gtatgaaggc ttttggtctc cctgggagtg ggtggaggca
     7321 gccagggctt acctgtacac tgacttgaga ccagttgaat aaaagtgcac accttaaaaa
     7381 tgaggccaag tgtgactttg tggtgtggct gggttggggg cagcagaggg tgaaccctgc
     7441 aggagggtga accctgcaaa agggtggggc agtgggggcc aacttgtcct cacccagagt
     7501 gcaggtgtgt ggagatccct cctgccttga cattgagcag ccttagaggg tgggggaggc
     7561 tcaggggtca ggtctctgtt cctgcttatt ggggagttcc tggcctggcc cttctatgtc
     7621 tccccaggta ccccagtttt tctgggttca cccagagtgc agatgcttga ggaggtggga
     7681 agggactatt tgggggtgtc tggctcaggt gccatgcctc actggggctg gttggcacct
     7741 gcatttcctg ggagtggggc tgtctcaggg tagctgggca cggtgttccc ttgagtgggg
     7801 gtgtagtgag tgttcctagc tgccacgcct ttgccttcac ctatgggatc gtggctgtca
     7861 gccttgaggg tcagcctggc ccaggctccc ataggcttag gagaggccgc aattcctacc
     7921 tgttcatcca gacagagggg gacctggaat caaagtcaag ttggggtggg gggtccatgg
     7981 ggccatatct ggcctgcaga cagctctggt tagctatggg ctgaggtctg gattctgcct
     8041 tgtgactgga gactgggcgc catcccgtgg cctctgaggg cccagtttgt tcgtgaccca
     8101 tgctgcagag ggagataccc aggctcctgg acctcagggc ctgcctggca ctgcaggcca
     8161 ggttctcttt ttatttctga gacggggtct tgtgttgccc aggctggagt gtaatggcac
     8221 aaccatggct cactgcagcc tcaactttgg gctcaagcaa ttctcctgcc tcagcctccc
     8281 aagtagctga aactacacag gcatgcccca gcacgcccag ctagttgcag gtcagattct
     8341 cattgttcta cagggccctg agttaaatac tctcgcaaga agaaaccaag cctggcctgc
     8401 cttccctctg ctagaagctg tgattgacac cagcagagaa tgaaggaaga aaagggggtg
     8461 aggatctatg cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc gcatcaggcg
     8521 ct
//
