LOCUS       sequence-44329-e        5316 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 5316)
  AUTHORS   Rosonina E, Blencowe BJ
  TITLE     Analysis of the requirement for RNA polymerase II CTD heptapeptide 
            repeats in pre-mRNA splicing and 3'-end cleavage.
  JOURNAL   RNA. 2004 Apr;10(4):581-9.
  PUBMED    15037767
REFERENCE   2  (bases 1 to 5316)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "RNA."; 
            date: "2004-04"; volume: "10(4)"; pages: "581-9"
COMMENT     Sequence Label: FLAG-Pol-II 31-52
FEATURES             Location/Qualifiers
     source          1..5316
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             4..27
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     regulatory      573..582
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      976..985
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 atggactaca aggacgacga tgacaaggct agcatgcacg ggggtggccc cccctcgggg
       61 gacagcgcat gcccgctgcg caccatcaag agagtccagt tcggagtcct gagtccggat
      121 gaactgaagc gaatgtctgt gacggagggt ggcatcaaat acccagagac gactgaggga
      181 ggccgcccca agcttggggg gctgatggac ccgaggcagg gggtgattga gcggactggc
      241 cgctgccaaa catgtgcagg aaacatgaca gagtgtcctg gccactttgg ccacattgaa
      301 ctggccaagc ctgtgtttca cgtgggcttc ctggtgaaga caatgaaagt tttgcgctgt
      361 gtctgcttct tctgctccaa actgcttgtg gactctaaca acccaaagat caaggatatc
      421 ctggctaagt ccaagggaca gcccaagaag cggctcacac atgtctacga cctttgcaag
      481 ggcaaaaaca tatgcgaggg tggggaggag atggacaaca agttcggtgt ggaacaacct
      541 gagggtgacg aggatctgac caaagaaaag ggccatggtg gctgtgggcg gtaccagccc
      601 aggatccggc gttctggcct agagttgtat gcggaatgga agcacgttaa tgaggactct
      661 caggagaaga agatcctgct gagtccagag cgagtgcatg agatcttcaa acgcatctca
      721 gatgaggagt gttttgtgct gggcatggag ccccgctatg cacggccaga gtggatgatt
      781 gtcacagtgc tgcctgtgcc cccgctctcc gtgcggcctg ctgttgtgat gcagggctct
      841 gcccgtaacc aggatgacct gactcacaaa ctggctgaca tcgtgaagat caacaatcag
      901 ctgcggcgca atgagcagaa cggcgcagcg gcccatgtca ttgcagagga tgtgaagctc
      961 ctccagttcc atgtggccac catggtggac aatgagctgc ctggcttgcc ccgtgccatg
     1021 cagaagtctg ggcgtcccct caagtccctg aagcagcggt tgaagggcaa ggaaggccgg
     1081 gtgcgaggga acctgatggg caaaagagtg gacttctcgg cccgtactgt catcaccccc
     1141 gaccccaacc tctccattga ccaggttggc gtgccccgct ccattgctgc caacatgacc
     1201 tttgcggaga ttgtcacccc cttcaacatt gacagacttc aagaactagt gcgcaggggg
     1261 aacagccagt acccaggcgc caagtacatc atccgagaca atggtgatcg cattgacttg
     1321 cgtttccacc ccaagcccag tgaccttcac ctgcagaccg gctataaggt ggaacggcac
     1381 atgtgtgatg gggacattgt tatcttcaac cggcagccaa ctctgcacaa aatgtccatg
     1441 atggggcatc gggtccgcat tctcccatgg tctacctttc gcttgaatct tagtgtgaca
     1501 actccgtaca atgcagactt tgacggggat gagatgaact tgcacctgcc acagtctctg
     1561 gagacgcgag cagagatcca ggagctggcc atggttcctc gcatgattgt caccccccag
     1621 agcaatcggc ctgtcatggg tattgtgcag gacacactca cagcagtgcg caaattcacc
     1681 aagagagacg tcttcctgga gcggggtgaa gtgatgaacc tcctgatgtt cctgtcgacg
     1741 tgggatggga aggtcccaca gccggccatc ctaaagcccc ggcccctgtg gacaggcaag
     1801 caaatcttct ccctcatcat acctggtcac atcaattgta tccgtaccca cagcacccat
     1861 cccgatgatg aagacagtgg cccttacaag cacatctctc ctggggacac caaggtggtg
     1921 gtggagaatg gggagctgat catgggcatc ctgtgtaaga agtctctggg cacgtcagct
     1981 ggctccctgg tccacatctc ctacctagag atgggtcatg acatcactcg cctcttctac
     2041 tccaacattc agactgtcat taacaactgg ctcctcatcg agggtcatac tattggcatt
     2101 ggggactcca ttgctgattc taagacttac caggacattc agaacactat taagaaggcc
     2161 aagcaggacg taatagaggt catcgagaag gcacacaaca atgagctgga gcccacccca
     2221 gggaacactc tgcggcagac gtttgagaat caggtgaacc gcattcttaa cgatgcccga
     2281 gacaagactg gctcctctgc tcagaaatcc ctgtctgaat acaacaactt caagtctatg
     2341 gtcgtgtccg gagctaaagg ttccaagatt aacatctccc aggtcattgc tgtcgttgga
     2401 cagcaggacg tcgagggcaa gcggattcca tttggcttca agcaccggac tctgcctcac
     2461 ttcatcaagg atgactacgg gcctgagagc cgtggctttg tggagaactc ctacctagcc
     2521 ggcctcacac ccactgagtt ctttttccac gccatggggg gtcgtgaggg gctcattgac
     2581 acggctgtca agactgctga gactggatac atccagcggc ggctgatcaa gtccatggag
     2641 tcagtgatgg tgaagtacga cgcgactgtg cggaactcca tcaaccaggt ggtgcagctg
     2701 cgctacggcg aagacggcct ggcaggcgag agcgttgagt tccagaacct ggctacgctt
     2761 aagccttcca acaaggcttt tgagaagaag ttccgctttg attataccaa tgagagggcc
     2821 ctgcggcgca ctctgcagga ggacctggtg aaggacgtgc tgagcaacgc acacatccag
     2881 aacgagttgg agcgggaatt tgagcggatg cgggaggatc gggaggtgct cagggtcatc
     2941 ttcccaactg gagacagcaa ggtcgtcctc ccctgtaacc tgctgcggat gatctggaat
     3001 gctcagaaaa tcttccacat caacccacgc cttccctccg acctgcaccc catcaaagtg
     3061 gtggagggag tcaaggaatt gagcaagaag ctggtgattg tgaatgggga tgacccacta
     3121 agtcgacagg cccaggaaaa tgccacgctg ctcttcaaca tccacctgcg gtccacgttg
     3181 tgttcccgcc gcatggcaga ggagtttcgg ctcagtgggg aggccttcga ctggctgctt
     3241 ggggagattg agtccaagtt caaccaagcc attgcgcatc ccggggaaat ggtgggggct
     3301 ctggctgcgc agtcccttgg agaacctgcc acccagatga ccttgaatac cttccactat
     3361 gctggtgtgt ctgccaagaa tgtgacgctg ggtgtgcccc gacttaagga gctcatcaac
     3421 atttccaaga agccaaagac tccttcgctt actgtcttcc tgttgggcca gtccgctcga
     3481 gatgctgaga gagccaagga tattctgtgc cgtctggagc atacaacgtt gaggaaggtg
     3541 actgccaaca cagccatcta ctatgacccc aacccccaga gcacggtggt ggcagaggat
     3601 caggaatggg tgaatgtcta ctatgaaatg cctgactttg atgtggcccg aatctccccc
     3661 tggctgttgc gggtggagct ggatcggaag cacatgactg accggaagct caccatggag
     3721 cagattgctg aaaagatcaa tgctggtttt ggtgacgact tgaactgcat ctttaatgat
     3781 gacaatgcag agaagctggt gctccgtatt cgcatcatga acagcgatga gaacaagatg
     3841 caagaggagg aagaggtggt ggacaagatg gatgatgatg tcttcctgcg ctgcatcgag
     3901 tccaacatgc tgacagatat gaccctgcag ggcatcgagc agatcagcaa ggtgtacatg
     3961 cacttgccac agacagacaa caagaagaag atcatcatca cggaggatgg ggaattcaag
     4021 gccctgcagg agtggatcct ggagacggac ggcgtgagct tgatgcgggt gctgagtgag
     4081 aaggacgtgg accccgtacg caccacgtcc aatgacattg tggagatctt cacggtgctg
     4141 ggcattgaag ccgtgcggaa ggccctggag cgggagctgt accacgtcat ctcctttgat
     4201 ggctcctatg tcaattaccg acacttggct ctcttgtgtg ataccatgac ctgtcgtggc
     4261 cacttgatgg ccatcacccg acacggagtc aaccgccagg acacaggacc actcatgaag
     4321 tgttcctttg aggaaacggt ggacgtgctt atggaagcag ccgcacacgg tgagagtgac
     4381 cccatgaagg gggtctctga gaatatcatg ctgggccagc tggctccggc cggcactggc
     4441 tgctttgacc tcctgcttga tgcagagaag tgcaagtatg gcatggagat ccccaccaat
     4501 atccccggcc tgggggctgc tggacccacc ggcatgttct ttggttcagc acccagtccc
     4561 atgggtggaa tctctcctgc catgacacct tggaaccagg gtgcaacccc tgcctatggc
     4621 gcctggtccc ccagtgttgg gagtggaatg accccagggg cagccggctt ctctcccagt
     4681 gctgcgtcag atgccagcgg cttcagccca ggttactccc ctgcctggtc tcccacaccg
     4741 ggctccccgg ggtccccagg tccctcaagc ccctacatcc cttcaccagg tggtgccatg
     4801 tctcccagct ctagacttct agattactcc ccttccagcc cacgatacac accacagtct
     4861 ccaacctata ccccaagctc acccagctac agccccagct cgcccagcta cagcccaacc
     4921 tcacccaagt acaccccaac cagtccttct tacagtccca gctccccaga gtatacccca
     4981 acctctccca agtactcacc taccagtccc aaatattcac ccacctctcc caagtactcg
     5041 cctaccagtc ccacctattc acccaccacc ccaaaatact ccccaacatc tcctacttat
     5101 tccccaacct ctccagtcta caccccaacc tctcccaagt actcacctac tagccccact
     5161 tactcgccca cttcccccaa gtactcgccc accagcccca cctactcgcc cacctccccc
     5221 aaaggctcaa cctactctcc cacttcccct ggttactcgc ccaccagccc cacctacagt
     5281 ctccaccagc ccggttatcg attaagcggc cgccta
//
