LOCUS       sequence-443291-        3027 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  low-copy plasmid for bacterial expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3027)
  AUTHORS   Manigat FO, Connell LB, Stewart BN, LePabic AR, Tessier CJG, Emlaw 
            JR, Calvert ND, Rossl A, Shuhendler AJ, daCosta CJB, Campbell-Valois
            FX
  TITLE     pUdOs: Concise Plasmids for Bacterial and Mammalian Cells.
  JOURNAL   ACS Synth Biol. 2024 Jan 18. doi: 10.1021/acssynbio.3c00408.
  PUBMED    38235654
REFERENCE   2  (bases 1 to 3027)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2024 Jan 18. doi: 10.1021/acssynbio.3c00408."
COMMENT     Sequence Label: pUdO4s
FEATURES             Location/Qualifiers
     source          1..3027
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(203..219)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(203..219)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             complement(220..882)
                     /codon_start=1
                     /product="replication protein for the broad-host-range 
                     plasmid pBBR1 from Bordetella bronchiseptica"
                     /label=pBBR1 Rep
                     /translation="MATQSREIGIQAKNKPGHWVQTERKAHEAWAGLIARKPTAAMLLH
                     HLVAQMGHQNAVVVSQKTLSKLIGRSLRTVQYAVKDLVAERWISVVKLNGPGTVSAYVV
                     NDRVAWGQPRDQLRLSVFSAAVVVDHDDQDESLLGHGDLRRIPTLYPGEQQLPTGPGEE
                     PPSQPGIPGMEPDLPALTETEEWERRGQQRLPMPDEPCFLDDGEPLEPPTRVTLPRR"
     rep_origin      883..1654
                     /label=pBBR1 oriV
                     /note="replication origin of the broad-host-range plasmid 
                     pBBR1 from Bordetella bronchiseptica; requires the pBBR1 
                     Rep protein for replication"
     primer_bind     1653..1673
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        1655..1673
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(1761..1780)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1762..1780)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             complement(1781..2572)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAAYWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     primer_bind     2890..2907
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2891..2907
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 cggtctagac ttcctgcagg gtggatccaa atcgatagac tagtacgcgg ccgcaattgt
       61 ttaaactggc tagcgagagc tcgcgaattc ggtctcgaac cgaagacact cggctcttcg
      121 gagcctttgg tcgaaaaaaa aagcccgcac tgtcaggtgc gggctttttt ctgtgtttcc
      181 gctagtcact tacccgggca tggtcatagc tgtttcctgc taccggcgcg gcagcgtgac
      241 ccgtgtcggc ggctccaacg gctcgccatc gtccagaaaa cacggctcat cgggcatcgg
      301 caggcgctgc tgcccgcgtc gttcccattc ctccgtttcg gtcaaggctg gcaggtctgg
      361 ttccatgccc ggaatgccgg gctggctggg cggctcctcg ccggggccgg tcggtagttg
      421 ctgctcgccc ggatacaggg tcgggatgcg gcgcaggtcg ccatgcccca acagcgattc
      481 gtcctggtcg tcgtgatcaa ccaccacggc ggcactgaac accgacaggc gcaactggtc
      541 gcggggctgg ccccacgcca cgcggtcatt gaccacgtag gccgacacgg tgccggggcc
      601 gttgagcttc acgacggaga tccagcgctc ggccaccaag tccttgactg cgtattggac
      661 cgtccgcaaa gaacgtccga tgagcttgga aagtgtcttc tggctgacca ccacggcgtt
      721 ctggtggccc atctgcgcca cgaggtgatg cagcagcatt gccgccgtgg gtttcctcgc
      781 aataagcccg gcccacgcct catgcgcttt gcgttccgtt tgcacccagt gaccgggctt
      841 gttcttggct tgaatgccga tttctctgga ctgcgtggcc atgcttatct ccatgcggta
      901 ggggtgccgc acggttgcgg caccatgcgc aatcagctgc aacttttcgg cagcgcgaca
      961 acaattatgc gttgcgtaaa agtggcagtc aattacagat tttctttaac ctacgcaatg
     1021 agctattgcg gggggtgccg caatgagctg ttgcgtaccc ccctttttta agttgttgat
     1081 ttttaagtct ttcgcatttc gccctatatc tagttctttg gtgcccaaag aagggcaccc
     1141 ctgcggggtt cccccacgcc ttcggcgcgg ctccccctcc ggcaaaaagt ggcccctccg
     1201 gggcttgttg atcgactgcg cggccttcgg ccttgcccaa ggtggcgctg cccccttgga
     1261 acccccgcac tcgccgccgt gaggctcggg gggcaggcgg gcgggcttcg cccttcgact
     1321 gcccccactc gcataggctt gggtcgttcc aggcgcgtca aggccaagcc gctgcgcggt
     1381 cgctgcgcga gccttgaccc gccttccact tggtgtccaa ccggcaagcg aagcgcgcag
     1441 gccgcaggcc ggaggctttt ccccagagaa aattaaaaaa attgatgggg caaggccgca
     1501 ggccgcgcag ttggagccgg tgggtatgtg gtcgaaggct gggtagccgg tgggcaatcc
     1561 ctgtggtcaa gctcgtgggc aggcgcagcc tgtccatcag cttgtccagc agggttgtcc
     1621 acgggccgag cgaagcgagc cagccggtgg ccgcaattaa ccctcactaa agggtcactt
     1681 acctaggaag gttgaaaaat aaaaacggcg ctaaaaagcg ccgttttttt tgacggtggt
     1741 aggcgcgcct caattagtta ccctatagtg agtcgtatta ttatttgccg actaccttgg
     1801 tgatctcgcc tttcacgtag tggacaaatt cttccaactg atctgcgcgc gaggccaagc
     1861 gatcttcttc ttgtccaaga taagcctgtc tagcttcaag tatgacgggc tgatactggg
     1921 ccggcaggcg ctccattgcc cagtaggcag cgacatcctt cggcgcgatt ttgccggtta
     1981 ctgcgctgta ccaaatgcgg gacaacgtaa gcactacatt tcgctcatcg ccagcccagt
     2041 cgggcggcga gttccatagc gttaaggttt catttagcgc ctcaaataga tcctgttcag
     2101 gaaccggatc aaagagttcc tccgccgctg gacctaccaa ggcaacgcta tgttctcttg
     2161 cttttgtcag caagatagcc agatcaatgt cgatcgtggc tggctcgaag atacctgcaa
     2221 gaatgtcatt gcgctgccat tctccaaatt gcagttcgcg cttagctgga taacgccacg
     2281 gaatgatgtc gtcgtgcaca acaatggtga cttctacagc gcggagaatc tcgctctctc
     2341 caggggaagc cgaagtttcc aaaaggtcgt tgatcaaagc tcgccgcgtt gtttcatcaa
     2401 gccttacggt caccgtaacc agcaaatcaa tatcactgtg tggcttcagg ccgccatcca
     2461 ctgcggagcc gtacaaatgt acggccagca acgtcggttc gagatggcgc tcgatgacgc
     2521 caactacctc tgatagttga gtcgatactt cggcgatcac cgcttccctc atgatgttta
     2581 actttgtttt agggcgactg ccctgctgcg taacatcgtt gctgctccat aacatcaaac
     2641 atcgacccac ggcgtaacgc gcttgctgct tggatgcccg aggcatagac tgtaccccaa
     2701 aaaaacagtc ataacaagcc atgaaaaccg ccactgcgcc gttaccaccg ctgcgttcgg
     2761 tcaaggttct ggaccagttg cgtgagcgca tacgctactt gcattacagc ttacgaaccg
     2821 aacaggctta tgtccactgg gttcgtgcct tcatccgttt ccacggtgtg cgtcacccgg
     2881 caaccttggt gtaaaacgac ggccagttaa gcttaggacc aaaacgaaaa aaggcccccc
     2941 tttcgggagg cctcttttct ggaatttggt accgagtcag ttacttacgt cgaagagcga
     3001 cgtgtcttct ctcagagacc gtgtcga
//
