LOCUS       sequence-443295-        2635 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  medium-copy plasmid for bacterial expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2635)
  AUTHORS   Manigat FO, Connell LB, Stewart BN, LePabic AR, Tessier CJG, Emlaw 
            JR, Calvert ND, Rossl A, Shuhendler AJ, daCosta CJB, Campbell-Valois
            FX
  TITLE     pUdOs: Concise Plasmids for Bacterial and Mammalian Cells.
  JOURNAL   ACS Synth Biol. 2024 Jan 18. doi: 10.1021/acssynbio.3c00408.
  PUBMED    38235654
REFERENCE   2  (bases 1 to 2635)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2024 Jan 18. doi: 10.1021/acssynbio.3c00408."
COMMENT     Sequence Label: pUdO3a
FEATURES             Location/Qualifiers
     source          1..2635
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..223
                     /label=pSC101 ori
                     /note="low-copy replication origin that requires the Rep101
                     protein"
     CDS             271..1221
                     /codon_start=1
                     /gene="rep101"
                     /product="RepA protein needed for replication with the 
                     pSC101 origin"
                     /label=Rep101
                     /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRKE
                     RTVSFTYNQYAQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFEIFQWTNYAKFS
                     SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI
                     EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV
                     ELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLRNGLRKTLHDALTAKIQLTSFEAKF
                     LSDMQSKYDLNGSFSWLTQKQRTTLENILAKYGRI"
     primer_bind     1222..1238
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1222..1238
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1561..1578)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(1561..1577)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        1577..1648
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1649..1717,1718..2509)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1867..1886)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     primer_bind     2510..2529
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2510..2528
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(2617..2635)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 gagttataca cagggctggg atctattctt tttatctttt tttattcttt ctttattcta
       61 taaattataa ccacttgaat ataaacaaaa aaaacacaca aaggtctagc ggaatttaca
      121 gagggtctag cagaatttac aagttttcca gcaaaggtct agcagaattt acagataccc
      181 acaactcaaa ggaaaaggac atgtaattat cattgactag cccatctcaa ttggtatagt
      241 gattaaaatc acctagacca attgagatgt atgtctgaat tagttgtttt caaagcaaat
      301 gaactagcga ttagtcgcta tgacttaacg gagcatgaaa ccaagctaat tttatgctgt
      361 gtggcactac tcaaccccac gattgaaaac cctacaagga aagaacggac ggtatcgttc
      421 acttataacc aatacgctca gatgatgaac atcagtaggg aaaatgctta tggtgtatta
      481 gctaaagcaa ccagagagct gatgacgaga actgtggaaa tcaggaatcc tttggttaaa
      541 ggctttgaga ttttccagtg gacaaactat gccaagttct caagcgaaaa attagaatta
      601 gtttttagtg aagagatatt gccttatctt ttccagttaa aaaaattcat aaaatataat
      661 ctggaacatg ttaagtcttt tgaaaacaaa tactccatga ggatttatga gtggttatta
      721 aaagaactaa cacaaaagaa aactcacaag gcaaatatag agattagcct tgatgaattt
      781 aagttcatgt taatgcttga aaataactac catgagttta aaaggcttaa ccaatgggtt
      841 ttgaaaccaa taagtaaaga tttaaacact tacagcaata tgaaattggt ggttgataag
      901 cgaggccgcc cgactgatac gttgattttc caagttgaac tagatagaca aatggatctc
      961 gtaaccgaac ttgagaacaa ccagataaaa atgaatggtg acaaaatacc aacaaccatt
     1021 acatcagatt cctacctacg taacggacta agaaaaacac tacacgatgc tttaactgca
     1081 aaaattcagc tcaccagttt tgaggcaaaa tttttgagtg acatgcaaag taagtatgat
     1141 ctcaatggtt cgttctcatg gctcacgcaa aaacaacgaa caacactaga gaacatactg
     1201 gctaaatacg gaaggatctg acaggaaaca gctatgacca tgcccgggta agtgactagc
     1261 ggaaacacag aaaaaagccc gcacctgaca gtgcgggctt tttttttcga ccaaaggctc
     1321 cgaagagccg agtgtcttcg gttcgagacc gaattcgcga gctctcgcta gccagtttaa
     1381 acaattgcgg ccgcgtacta gtctatcgat ttggatccac cctgcaggaa gtctagaccg
     1441 tcgacacggt ctctgagaga agacacgtcg ctcttcgacg taagtaactg actcggtacc
     1501 aaattccaga aaagaggcct cccgaaaggg gggccttttt tcgttttggt cctaagctta
     1561 actggccgtc gttttacatt caaatatgta tccgctcatg agacaataac cctgataaat
     1621 gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat
     1681 tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt
     1741 aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag
     1801 cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa
     1861 agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg
     1921 ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct
     1981 tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac
     2041 tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca
     2101 caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat
     2161 accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact
     2221 attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc
     2281 ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga
     2341 taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg
     2401 taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg
     2461 aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaat aatacgactc
     2521 actatagggt aactaattga ggcgcgccta ccaccgtcaa aaaaaacggc gctttttagc
     2581 gccgttttta tttttcaacc ttcctaggta agtgaccctt tagtgagggt taatt
//
