LOCUS       sequence-443327-       10902 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral mCherry-CLCN3 expression vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10902)
  AUTHORS   Gonzalez-Lozano MA, Schmid EW, Miguel Whelan E, Jiang Y, Paulo JA, 
            Walter JC, Harper JW
  TITLE     EndoMAP.v1 charts the structural landscape of human early endosome 
            complexes.
  JOURNAL   Nature. 2025 Jul;643(8070):252-261. doi: 10.1038/s41586-025-09059-y.
            Epub 2025 May 28.
  PUBMED    40437099
REFERENCE   2  (bases 1 to 10902)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41586-025-09059-y"; journalName: "Nature"; date: 
            "2025-07"; volume: "643"; issue: "8070"; pages: "252-261"
COMMENT     Sequence Label: pHAGE-mCherry-CLCN3
FEATURES             Location/Qualifiers
     source          1..10902
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(93..110)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(264..852)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(344..363)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1023..1814,1815..1883))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1646..1665
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1884..1988)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2056..2074
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     LTR             2193..2426
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     misc_feature    2501..3089
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             2607..2618
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     3016..3036
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3106..3705)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(3189..3209)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     3686..3705
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     misc_feature    3717..4269
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    3719..4286
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(3879..3898)
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     primer_bind     4108..4125
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     protein_bind    4299..4323
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(6784..6808)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(6833..7540)
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(7108..7127)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     7377..7395
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     complement(7622..7646)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        complement(7643..7846)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(7672..7692)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        7847..8150
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     misc_feature    8202..8319
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(8616..8660)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    8845..9078
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    9575..9700
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             9747..10380
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(10572..10591)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(10641..10657)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10641..10657)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(10654..10676)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    10665..10681
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(10689..10695,10696..10713,10714..10719))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10734..10755
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 aaaaaaaatt agtcagccat gagcttgctg cattaatgaa tcggccaacg cgcggggaga
       61 ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc
      121 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa
      181 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt
      241 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa
      301 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
      361 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
      421 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc
      481 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
      541 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
      601 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
      661 acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc
      721 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
      781 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
      841 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa
      901 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt
      961 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac
     1021 agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc
     1081 atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc
     1141 cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata
     1201 aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc
     1261 cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc
     1321 aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca
     1381 ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa
     1441 gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca
     1501 ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt
     1561 tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt
     1621 tgctcttgcc cggcgtcaac acgggataat accgcgccac atagcagaac tttaaaagtg
     1681 ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga
     1741 tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc
     1801 agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg
     1861 acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag
     1921 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
     1981 gttccgcgca catttccccg aaaagtgcca cctgacgtct aagaaaccat tattatcatg
     2041 acattaacct ataaaaatag gcgtatcacg aggccctttc gtcttcaaga atgatctagc
     2101 cctttcctta attaacccgg gcctctcact ctctgatatt catttctttg caagttataa
     2161 atactgaata ataagatgac atgaactact actgctagag attttccaca ctgactaaaa
     2221 gggtctgagg gatctctagt taccagagtc acacaacaga cgggcacaca ctacttgaag
     2281 cactcaaggc aagctttatt gaggcttaag cagtgggttc cctagttagc cagagagctc
     2341 ccaggctcag atctggtcta accagagaga cccagtacaa gcaaaaagca gatcttgtct
     2401 tcgttgggag tgaattagcc cttccagtcc ccccttttct tttaaaaagt ggctaagatc
     2461 tacagctgcc ttgtaagtca ttggtcttaa aggatctcag gcggggaggc ggcccaaagg
     2521 gagatccgac tcgtctgagg gcgaaggcga agacgcggaa gaggccgcag agccggcagc
     2581 aggccgcggg aaggaaggtc cgctggattg agggccgaag ggacgtagca gaaggacgtc
     2641 ccgcgcagaa tccaggtggc aacacaggcg agcagccaag gaaaggacga tgatttcccc
     2701 gacaacacca cggaattgtc agtgcccaac agccgagccc ctgtccagca gcgggcaagg
     2761 caggcggcga tgagttccgc cgtggcaata gggaggggga aagcgaaagt cccggaaagg
     2821 agctgacagg tggtggcaat gccccaacca gtgggggttg cgtcagcaaa cacagtgcac
     2881 accacgccac gttgcctgac aacgggccac aactcctcat aaagagacag caaccaggat
     2941 ttatacaagg aggagaaaat gaaagccata cgggaagcaa tagcatgata caaaggcatt
     3001 aaagcagcgt atccacatag cgtaaaagga gcaacatagt taagaatacc agtcaatctt
     3061 tcacaaattt tgtaatccag aggttgatta ggatccatcg atgcatcagg caccgggctt
     3121 gcgggtcatg caccaggtgc gcggtccttc gggcacctcg acgtcggcgg tgacggtgaa
     3181 gccgagccgc tcgtagaagg ggaggttgcg gggcgcggag gtctccagga aggcgggcac
     3241 cccggcgcgc tcggccgcct ccactccggg gagcacgacg gcgctgccca gacccttgcc
     3301 ctggtggtcg ggcgagacgc cgacggtggc caggaaccac gcgggctcct tgggccggtg
     3361 cggcgccagg aggccttcca tctgttgctg cgcggccagc cgggaaccgc tcaactcggc
     3421 catgcgcggg ccgatctcgg cgaacaccgc ccccgcttcg acgctctccg gcgtggtcca
     3481 gaccgccacc gcggcgccgt cgtccgcgac ccacaccttg ccgatgtcga gcccgacgcg
     3541 cgtgaggaag agttcttgca gctcggtgac ccgctcgatg tggcggtccg gatcgacggt
     3601 gtggcgcgtg gcggggtagt cggcgaacgc ggcggcgagg gtgcgtacgg ccctggggac
     3661 gtcgtcgcgg gtggcgaggc gcaccgtggg cttgtactcg gtcatggttg tggccatatt
     3721 atcatcgtgt ttttcaaagg aaaaccacgt ccccgtggtt cggggggcct agacgttttt
     3781 ttaacctcga ctaaacacat gtaaagcatg tgcaccgagg ccccagatca gatcccatac
     3841 aatggggtac cttctgggca tccttcagcc ccttgttgaa tacgcttgag gagagccatt
     3901 tgactctttc cacaactatc caactcacaa cgtggcactg gggttgtgcc gcctttgcag
     3961 gtgtatctta tacacgtggc ttttggccgc agaggcacct gtcgccaggt ggggggttcc
     4021 gctgcctgca aagggtcgct acagacgttg tttgtcttca agaagcttcc agaggaactg
     4081 cttccttcac gacattcaac agaccttgca ttcctttggc gagaggggaa agacccctag
     4141 gaatgctcgt caagaagaca gggccaggtt tccgggccct cacattgcca aaagacggca
     4201 atatggtgga aaataacata tagacaaacg cacaccggcc ttattccaag cggcttcggc
     4261 cagtaacgtt aggggggggg ggcggaattc gttatcgaac cactttgtac aagaaagctg
     4321 ggtcgccgtt gaacattatt gaagcggggt cttggtttgc cgtctgggcc atatgccgga
     4381 ggatatcttt ttttgttata atgccaagga ggcgcccatt gtgagttaca aggcactgcc
     4441 tcagtcccag ctttcggaaa atatccacca cgatctccat tggggtgtgg tctgtcactg
     4501 taaaagggct catgtcaaga atgcttcgaa gcttcaatgg ccgaggactt tctgctggaa
     4561 gagatggggt gtgctgtgca aaacacaccc gagaactgcc aacgatacct tctcgttttt
     4621 tcctggcact ttctattgca attgtcaggt ctcttctgag ggcaaatccc actaatctct
     4681 gagattcttt tgacattatg acaggaaatc cattgtagct ggtttcatta atcatgtttt
     4741 ctatatcatc cactgtcata ttgtcctgtg tcaggacagc taagggagga tcattccttc
     4801 gaggtctcat aacgtcagca gccagggtgg tatgagtgaa ttcttctttt gcatccaaga
     4861 aggggtatcc atttaatcgg atgtgtgctt cataaatgcc ttccctgcca aaggcatctc
     4921 caacccattt actggtcatg actgcagcca taaggggaac aatatattcc aagcctccag
     4981 taagctcaaa aacaataacc accagggaga cagtcattct tgtcacacca cctaagcatg
     5041 cagcagcacc aaccatggca taaaggccag gtgcaatgca atcagccccg acctcacacc
     5101 actccttaaa gataaaccag tcgtggtgac agtaggcaag ctgctccacc gcaatcccca
     5161 caatccttcc tgcgatcgct ccaatggcca tgctggggat gaacaagcct gatggaacct
     5221 tgatgccaaa agtgaatact gtcattatga ttttaaatat gagtgccagg cataactgcc
     5281 atatagctga atatactcca atgcctgctg gacgatcagg aatgtcatcg acaattttac
     5341 tggcattcat gtcatttctg tagtcacaaa gagaagagga ttccagggga ccacagtctg
     5401 taaaaagctc tttgatcagt tcactggtgt ttagcctagt gtatggatta gggaaggcta
     5461 tcacagcagt aatggctgca acaataatga cttccagaac gggatacttt ccaaatttcg
     5521 tggacttgcg tcgacgacac caggcaatat ttgccctaat gaaaaaggct ccccaaagcc
     5581 ctccaaatac ccctagaaga ataaaaggaa acagttcaaa aaggtaccat ggtgtatgat
     5641 actccacata aaaaaggacc agacggctgt taccaaatgg attgatggac ctcaaaacaa
     5701 atgcagccac taaagcagca aaaaatgatc tccataaagt tttgagagga aaataatagc
     5761 taacctcttc caggctaaaa agaactcctc caattggtgc accaaaagct acagaaaccc
     5821 ctgcagctga ggcagctgat agcacctccc tttttttagc ttcgtttgtg ctatactttg
     5881 gaaagaggta ggaaaagata tttccgcagc aacaggcaac atgtaccagg ggaccttctt
     5941 ttcctaaact caaacctgat gccacagcca ggactaatgt gatggtttta atcattaaag
     6001 tccattttcc caagtaacct ctgatgatga atccacttaa aatagtttta atctctggaa
     6061 ttccagagcc acaggcatat ggagcaaata cctttaccag ggaaactgca agaaaggcaa
     6121 aactcaaggc ccagaagatg tacattatgt agttcgtgat ataagaacca ggaccctctg
     6181 cttgacctat gattaattct gcccatgttt tccactgtgg acatttatcc ctctcttcaa
     6241 atgttgtttc attagatccc caacagcact gttcgtggtt gtaccacaac gcactaaggc
     6301 aaatgccctc ctttaggtca gtcatccaat cggcagcaat gtctattaat ccggccagtg
     6361 cccctgatgc caatcctgtt agtgttacta ctagccatcc tgaccacgca tcatacaaac
     6421 tttttgtcat ttcccatgct gattctttct ttttgctgtt gatccgtcta tgcctttctc
     6481 tgtctttaca tttttctcgc acccaatcaa tagtatggaa atcatcatat gtaccaacac
     6541 ctggaattgg ttcatccaaa agatccagta aatgtgtaga actgttaatg ctgcctccat
     6601 ttgtcattgt ataatgagtt ccaactgcag tgtcaccatc taataaattg tcatcttcag
     6661 atgtttgaaa gtccataata acacctgctc catctaaaag ttcctcatca ctacttgcac
     6721 ttgttatact gttgtagctg tttctatagt agcctctatg gaacagctgc tcagactcca
     6781 tggtgccagc ttttttgtac aaacttgttt gataagcact agcgctgcat gccttgtaca
     6841 gctcgtccat gccgccggtg gagtggcggc cctcggcgcg ttcgtactgt tccacgatgg
     6901 tgtagtcctc gttgtgggag gtgatgtcca acttgatgtt gacgttgtag gcgccgggca
     6961 gctgcacggg cttcttggcc ttgtaggtgg tcttgacctc agcgtcgtag tggccgccgt
     7021 ccttcagctt cagcctctgc ttgatctcgc ccttcagggc gccgtcctcg gggtacatcc
     7081 gctcggagga ggcctcccag cccatggtct tcttctgcat tacggggccg tcggagggga
     7141 agttggtgcc gcgcagcttc accttgtaga tgaactcgcc gtcctgcagg gaggagtcct
     7201 gggtcacggt caccacgccg ccgtcctcga agttcatcac gcgctcccac ttgaagccct
     7261 cggggaagga cagcttcaag tagtcgggga tgtcggcggg gtgcttcacg taggccttgg
     7321 agccgtacat gaactgaggg gacaggatgt cccaggcgaa gggcaggggg ccacccttgg
     7381 tcaccttcag cttggcggtc tgggtgccct cgtaggggcg gccctcgccc tcgccctcga
     7441 tctcgaactc gtggccgttc acggagccct ccatgtgcac cttgaagcgc atgaactcct
     7501 tgatgatggc catgttatcc tcctcgccct tgctcaccat gctagcagat caacctcgag
     7561 agatctgcgg ccgccggtgt cttctatgga ggtcaaaaca gcgtggatgg cgtctccagg
     7621 cgatctgacg gttcactaaa cgagctctgc ttatatagac ctcccaccgt acacgcctac
     7681 cgcccatttg cgtcaatggg gcggagttgt tacgacattt tggaaagtcc cgttgatttt
     7741 ggtgccaaaa caaactccca ttgacgtcaa tggggtggag acttggaaat ccccgtgagt
     7801 caaaccgcta tccacgccca ttgatgtact gccaaaaccg catcaccatg gtaatagcga
     7861 tgactaatac gtagatgtac tgccaagtag gaaagtccca taaggtcatg tactgggcat
     7921 aatgccaggc gggccattta ccgtcattga cgtcaatagg gggcgtactt ggcatatgat
     7981 acacttgatg tactgccaag tgggcagttt accgtaaata ctccacccat tgacgtcaat
     8041 ggaaagtccc tattggcgtt actatgggaa catacgtcat tattgacgtc aatgggcggg
     8101 ggtcgttggg cggtcagcca ggcgggccat ttaccgtaag ttatgtaacg cggaactcca
     8161 ctagtccaaa ctggatctct gctgtccctg taataaaccc gaaaattttg aatttttgta
     8221 atttgttttt gtaattcttt agtttgtatg tctgttgcta ttatgtctac tattctttcc
     8281 cctgcactgt accccccaat cccccctttt cttttaaaat tgtggatgaa tactgccatt
     8341 tgtgaattaa ttcggcgata ccgtcgagat ccgttcacta atcgaatgga tctgtctctg
     8401 tctctctctc caccttcttc ttctattcct tcgggcctgt cgggtcccct cggggttggg
     8461 aggtgggtct gaaacgataa tggtgaatat ccctgcctaa ctctattcac tatagaaagt
     8521 acagcaaaaa ctattcttaa acctaccaag cctcctacta tcattatgaa taattttata
     8581 taccacagcc aatttgttat gttaaaccaa ttccacaaac ttgcccattt atctaattcc
     8641 aataattctt gttcattctt ttcttgctgg ttttgcgatt cttcaattaa ggagtgtatt
     8701 aagcttgtgt aattgttaat ttctctgtcc cactccatcc aggtcgtgtg attccaaatc
     8761 tgttccagag atttattact ccaactagca ttccaaggca cagcagtggt gcaaatgagt
     8821 tttccagagc aaccccaaat ccccaggagc tgttgatcct ttaggtatct ttccacagcc
     8881 aggattcttg cctggagctg cttgatgccc cagactgtga gttgcaacag atgctgttgc
     8941 gcctcaatag ccctcagcaa attgttctgc tgctgcacta taccagacaa taattgtctg
     9001 gcctgtaccg tcagcgtcat tgacgctgcg cccatagtgc ttcctgctgc tcccaagaac
     9061 ccaaggaaca aagctcctat tcccactgct cttttttctc tctgcaccac tcttctcttt
     9121 gccttggtgg gtgctactcc taatggttca atttttacta ctttatattt atataattca
     9181 cttctccaat tgtccctcat atctcctcct ccaggtctga agatcagcgg ccggccgctt
     9241 gctgtgcggt ggtcttactt ttgttttgct cttcctctat cttgtctaaa gcttccttgg
     9301 tgtcttttat ctctatcctt tgatgcacac aatagagggt tgctactgta ttatataatg
     9361 atctaagttc ttctgatcct gtctgaaggg atggttgtag ctgtcccagt atttgtctac
     9421 agccttctga tgtttctaac aggccaggat taactgcgaa tcgttctagc tccctgcttg
     9481 cccatactat atgttttaat ttatattttt tctttccccc tggccttaac cgaatttttt
     9541 cccatcgcga tctaattctc ccccgcttaa tactgacgct ctcgcaccca tctctctcct
     9601 tctagcctcc gctagtcaaa atttttggcg tactcaccag tcgccgcccc tcgcctcttg
     9661 ccgtgcgcgc ttcagcaagc cgagtcctgc gtcgagagag ctcctctggt ttccctttcg
     9721 ctttcaagtc cctgttcggg cgccactgct agagattttc cacactgact aaaagggtct
     9781 gagggatctc tagttaccag agtcacacaa cagacgggca cacactactt gaagcactca
     9841 aggcaagctt tattgaggct taagcagtgg gttccctagt tagccagaga gctcccaggc
     9901 tcagatctgg tctaaccaga gagacccagt acaggcaaaa agcagctgct tatatgcagg
     9961 atctgagggc tcgccactcc ccagtcccgc ccaggccacg cctccctgga aagtccccag
    10021 cggaaagtcc cttgtagcaa gctcgatatc agcagttctt gaagtactcc ggatgcagct
    10081 ctcgggccac gtgatgaaat gctaggcggc tgtcaaacct ccactctaac acttctctct
    10141 ccgggtcatc catcccatgc aggctcacag ggtgtaacaa gctggtgttc tctcctttat
    10201 tggcctcttc taccttatct ggctcaactg gtactagctt gtagcaccat ccaaaggtca
    10261 gtggatatct gacccctggc cctggtgtgt agttctgcta atcagggaag tagccttgtg
    10321 tgtggtagat ccacagatca aggatatctt gtcttctttg ggagtgaatt agcccttcca
    10381 actactaagt ttgtagtaca tatttaacaa atacaatttc tttaaaatga aaataattca
    10441 gaggaatcac aggtttagag taaatgaaac cacaggtaat tggcagtggt aatagggtat
    10501 ggggtgggaa gtttgggatg attttggtta gcttgagtta tccagttgat ccagacatga
    10561 taagatacat tgatgagttt ggacaaacca caactagaat gcagtgaaaa aaatgcttta
    10621 tttgtgaaat tcgtaatcat gtcatagctg tttcctgtgt gaaattgtta tccgctcaca
    10681 attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg
    10741 agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg
    10801 tgccacgggg agctttttgc aaaagcctag gcctccaaaa aagcctcctc actacttctg
    10861 gaatagctca gaggccgagg cggcctcggc ctctgcataa at
//
