LOCUS       sequence-443328-        9039 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral TMEM9-EGFP expression vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9039)
  AUTHORS   Gonzalez-Lozano MA, Schmid EW, Miguel Whelan E, Jiang Y, Paulo JA, 
            Walter JC, Harper JW
  TITLE     EndoMAP.v1 charts the structural landscape of human early endosome 
            complexes.
  JOURNAL   Nature. 2025 Jul;643(8070):252-261. doi: 10.1038/s41586-025-09059-y.
            Epub 2025 May 28.
  PUBMED    40437099
REFERENCE   2  (bases 1 to 9039)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41586-025-09059-y"; journalName: "Nature"; date: 
            "2025-07"; volume: "643"; issue: "8070"; pages: "252-261"
COMMENT     Sequence Label: pHAGE-TMEM9-EGFP
FEATURES             Location/Qualifiers
     source          1..9039
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     743..760
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    1000..1021
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(1036..1041,1042..1059,1060..1066)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1074..1090
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     1079..1101
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     1098..1114
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1098..1114
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     1164..1183
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             1375..2008
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    2055..2180
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    2677..2910
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             3095..3139
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    3436..3553
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        3605..3908
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3909..4112
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4063..4083
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     4109..4133
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     4208..4227
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        4208..4226
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    4256..4280
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(4830..4854)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             join(4886..4888,4889..5602)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     CDS             join(4886..4888,4889..5599)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(4928..4949)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(5189..5208)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5536..5557
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    5606..6173
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    5623..6175
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5767..5784)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5994..6013
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             6187..6786
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6187..6206)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6683..6703
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    6803..7391
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6856..6876)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7274..7285)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7466..7699
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(7818..7836)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7904..8008
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8009..8077,8078..8869)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8227..8246)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
      781 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagcaagc tcatggctga
      841 ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag
      901 tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agctccccgt ggcacgacag
      961 gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt agctcactca
     1021 ttaggcaccc caggctttac actttatgct tccggctcgt atgttgtgtg gaattgtgag
     1081 cggataacaa tttcacacag gaaacagcta tgacatgatt acgaatttca caaataaagc
     1141 atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt
     1201 ctggatcaac tggataactc aagctaacca aaatcatccc aaacttccca ccccataccc
     1261 tattaccact gccaattacc tgtggtttca tttactctaa acctgtgatt cctctgaatt
     1321 attttcattt taaagaaatt gtatttgtta aatatgtact acaaacttag tagttggaag
     1381 ggctaattca ctcccaaaga agacaagata tccttgatct gtggatctac cacacacaag
     1441 gctacttccc tgattagcag aactacacac cagggccagg ggtcagatat ccactgacct
     1501 ttggatggtg ctacaagcta gtaccagttg agccagataa ggtagaagag gccaataaag
     1561 gagagaacac cagcttgtta caccctgtga gcctgcatgg gatggatgac ccggagagag
     1621 aagtgttaga gtggaggttt gacagccgcc tagcatttca tcacgtggcc cgagagctgc
     1681 atccggagta cttcaagaac tgctgatatc gagcttgcta caagggactt tccgctgggg
     1741 actttccagg gaggcgtggc ctgggcggga ctggggagtg gcgagccctc agatcctgca
     1801 tataagcagc tgctttttgc ctgtactggg tctctctggt tagaccagat ctgagcctgg
     1861 gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg
     1921 cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc
     1981 ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa cagggacttg aaagcgaaag
     2041 ggaaaccaga ggagctctct cgacgcagga ctcggcttgc tgaagcgcgc acggcaagag
     2101 gcgaggggcg gcgactggtg agtacgccaa aaattttgac tagcggaggc tagaaggaga
     2161 gagatgggtg cgagagcgtc agtattaagc gggggagaat tagatcgcga tgggaaaaaa
     2221 ttcggttaag gccaggggga aagaaaaaat ataaattaaa acatatagta tgggcaagca
     2281 gggagctaga acgattcgca gttaatcctg gcctgttaga aacatcagaa ggctgtagac
     2341 aaatactggg acagctacaa ccatcccttc agacaggatc agaagaactt agatcattat
     2401 ataatacagt agcaaccctc tattgtgtgc atcaaaggat agagataaaa gacaccaagg
     2461 aagctttaga caagatagag gaagagcaaa acaaaagtaa gaccaccgca cagcaagcgg
     2521 ccggccgctg atcttcagac ctggaggagg agatatgagg gacaattgga gaagtgaatt
     2581 atataaatat aaagtagtaa aaattgaacc attaggagta gcacccacca aggcaaagag
     2641 aagagtggtg cagagagaaa aaagagcagt gggaatagga gctttgttcc ttgggttctt
     2701 gggagcagca ggaagcacta tgggcgcagc gtcaatgacg ctgacggtac aggccagaca
     2761 attattgtct ggtatagtgc agcagcagaa caatttgctg agggctattg aggcgcaaca
     2821 gcatctgttg caactcacag tctggggcat caagcagctc caggcaagaa tcctggctgt
     2881 ggaaagatac ctaaaggatc aacagctcct ggggatttgg ggttgctctg gaaaactcat
     2941 ttgcaccact gctgtgcctt ggaatgctag ttggagtaat aaatctctgg aacagatttg
     3001 gaatcacacg acctggatgg agtgggacag agaaattaac aattacacaa gcttaataca
     3061 ctccttaatt gaagaatcgc aaaaccagca agaaaagaat gaacaagaat tattggaatt
     3121 agataaatgg gcaagtttgt ggaattggtt taacataaca aattggctgt ggtatataaa
     3181 attattcata atgatagtag gaggcttggt aggtttaaga atagtttttg ctgtactttc
     3241 tatagtgaat agagttaggc agggatattc accattatcg tttcagaccc acctcccaac
     3301 cccgagggga cccgacaggc ccgaaggaat agaagaagaa ggtggagaga gagacagaga
     3361 cagatccatt cgattagtga acggatctcg acggtatcgc cgaattaatt cacaaatggc
     3421 agtattcatc cacaatttta aaagaaaagg ggggattggg gggtacagtg caggggaaag
     3481 aatagtagac ataatagcaa cagacataca aactaaagaa ttacaaaaac aaattacaaa
     3541 aattcaaaat tttcgggttt attacaggga cagcagagat ccagtttgga ctagtggagt
     3601 tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc
     3661 cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac
     3721 gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata
     3781 tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc
     3841 agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta
     3901 ttaccatggt gatgcggttt tggcagtaca tcaatgggcg tggatagcgg tttgactcac
     3961 ggggatttcc aagtctccac cccattgacg tcaatgggag tttgttttgg caccaaaatc
     4021 aacgggactt tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc
     4081 gtgtacggtg ggaggtctat ataagcagag ctcgtttagt gaaccgtcag atcgcctgga
     4141 gacgccatcc acgctgtttt gacctccata gaagacaccg gcggccgcag atctctcgag
     4201 gttgatctaa tacgactcac tatagggaga cccaagctgg ctagttaagc tatcaacaag
     4261 tttgtacaaa aaagttggca tgaagctctt atctttggtg gctgtggtcg ggtgtttgct
     4321 ggtgccccca gctgaagcca acaagagttc tgaagatatc cggtgcaaat gcatctgtcc
     4381 accttataga aacatcagtg ggcacattta caaccagaat gtatcccaga aggactgcaa
     4441 ctgcctgcac gtggtggagc ccatgccagt gcctggccat gacgtggagg cctactgcct
     4501 gctgtgcgag tgcaggtacg aggagcgcag caccaccacc atcaaggtca tcattgtcat
     4561 ctacctgtcc gtggtgggtg ccctgttgct ctacatggcc ttcctgatgc tggtggaccc
     4621 tctgatccga aagccggatg catacactga gcaactgcac aatgaggagg agaatgagga
     4681 tgctcgctct atggcagcag ctgctgcatc cctcggggga ccccgagcaa acacagtcct
     4741 ggagcgtgtg gaaggtgccc agcagcggtg gaagctgcag gtgcaggagc agcggaagac
     4801 agtcttcgat cggcacaaga tgctcagcta cccaactttc ttgtacaaag tggttgatct
     4861 agagggcccg cggttcgaac tcgaggtgag caagggcgag gagctgttca ccggggtggt
     4921 gcccatcctg gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga
     4981 gggcgagggc gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa
     5041 gctgcccgtg ccctggccca ccctcgtgac caccctgacc tacggcgtgc agtgcttcag
     5101 ccgctacccc gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta
     5161 cgtccaggag cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt
     5221 gaagttcgag ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga
     5281 ggacggcaac atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat
     5341 catggccgac aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga
     5401 ggacggcagc gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc
     5461 cgtgctgctg cccgacaacc actacctgag cacccagtcc gccctgagca aagaccccaa
     5521 cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg
     5581 catggacgag ctgtacaagt agaattccgc cccccccccc taacgttact ggccgaagcc
     5641 gcttggaata aggccggtgt gcgtttgtct atatgttatt ttccaccata ttgccgtctt
     5701 ttggcaatgt gagggcccgg aaacctggcc ctgtcttctt gacgagcatt cctaggggtc
     5761 tttcccctct cgccaaagga atgcaaggtc tgttgaatgt cgtgaaggaa gcagttcctc
     5821 tggaagcttc ttgaagacaa acaacgtctg tagcgaccct ttgcaggcag cggaaccccc
     5881 cacctggcga caggtgcctc tgcggccaaa agccacgtgt ataagataca cctgcaaagg
     5941 cggcacaacc ccagtgccac gttgtgagtt ggatagttgt ggaaagagtc aaatggctct
     6001 cctcaagcgt attcaacaag gggctgaagg atgcccagaa ggtaccccat tgtatgggat
     6061 ctgatctggg gcctcggtgc acatgcttta catgtgttta gtcgaggtta aaaaaacgtc
     6121 taggcccccc gaaccacggg gacgtggttt tcctttgaaa aacacgatga taatatggcc
     6181 acaaccatga ccgagtacaa gcccacggtg cgcctcgcca cccgcgacga cgtccccagg
     6241 gccgtacgca ccctcgccgc cgcgttcgcc gactaccccg ccacgcgcca caccgtcgat
     6301 ccggaccgcc acatcgagcg ggtcaccgag ctgcaagaac tcttcctcac gcgcgtcggg
     6361 ctcgacatcg gcaaggtgtg ggtcgcggac gacggcgccg cggtggcggt ctggaccacg
     6421 ccggagagcg tcgaagcggg ggcggtgttc gccgagatcg gcccgcgcat ggccgagttg
     6481 agcggttccc ggctggccgc gcagcaacag atggaaggcc tcctggcgcc gcaccggccc
     6541 aaggagcccg cgtggttcct ggccaccgtc ggcgtctcgc ccgaccacca gggcaagggt
     6601 ctgggcagcg ccgtcgtgct ccccggagtg gaggcggccg agcgcgccgg ggtgcccgcc
     6661 ttcctggaga cctccgcgcc ccgcaacctc cccttctacg agcggctcgg cttcaccgtc
     6721 accgccgacg tcgaggtgcc cgaaggaccg cgcacctggt gcatgacccg caagcccggt
     6781 gcctgatgca tcgatggatc ctaatcaacc tctggattac aaaatttgtg aaagattgac
     6841 tggtattctt aactatgttg ctccttttac gctatgtgga tacgctgctt taatgccttt
     6901 gtatcatgct attgcttccc gtatggcttt cattttctcc tccttgtata aatcctggtt
     6961 gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt
     7021 gtttgctgac gcaaccccca ctggttgggg cattgccacc acctgtcagc tcctttccgg
     7081 gactttcgct ttccccctcc ctattgccac ggcggaactc atcgccgcct gccttgcccg
     7141 ctgctggaca ggggctcggc tgttgggcac tgacaattcc gtggtgttgt cggggaaatc
     7201 atcgtccttt ccttggctgc tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt
     7261 ctgctacgtc ccttcggccc tcaatccagc ggaccttcct tcccgcggcc tgctgccggc
     7321 tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg agtcggatct ccctttgggc
     7381 cgcctccccg cctgagatcc tttaagacca atgacttaca aggcagctgt agatcttagc
     7441 cactttttaa aagaaaaggg gggactggaa gggctaattc actcccaacg aagacaagat
     7501 ctgctttttg cttgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct
     7561 ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta
     7621 gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca
     7681 gtgtggaaaa tctctagcag tagtagttca tgtcatctta ttattcagta tttataactt
     7741 gcaaagaaat gaatatcaga gagtgagagg cccgggttaa ttaaggaaag ggctagatca
     7801 ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat
     7861 aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg
     7921 tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat
     7981 gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat
     8041 tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt
     8101 aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag
     8161 cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa
     8221 agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg
     8281 ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct
     8341 tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac
     8401 tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca
     8461 caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat
     8521 accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact
     8581 attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc
     8641 ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga
     8701 taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg
     8761 taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg
     8821 aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca
     8881 agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta
     8941 ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca
     9001 ctgagcgtca gaccccgtag aaaagatcaa aggatcttc
//
