LOCUS       sequence-443345-        2388 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Dual reporter of Ca2+ levels and AMPK activity.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2388)
  AUTHORS   Erdogan YC, Pilic J, Gottschalk B, Yigit EN, Zaki AG, Ozturk G, 
            Eroglu E, Okutan B, Sommer NG, Weinberg AM, Schindl R, Graier WF, 
            Malli R
  TITLE     Development of a Dual Reporter System to Simultaneously Visualize 
            Ca(2+) Signals and AMPK Activity.
  JOURNAL   ACS Sens. 2024 Aug 21. doi: 10.1021/acssensors.4c01058.
  PUBMED    39167044
REFERENCE   2  (bases 1 to 2388)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Sens. 
            2024 Aug 21. doi: 10.1021/acssensors.4c01058."
COMMENT     Sequence Label: GCaMP-AMPK-SPARK
FEATURES             Location/Qualifiers
     source          1..2388
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             157..174
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             join(214..222,223..237)
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
     CDS             265..321
                     /codon_start=1
                     /product="calmodulin-binding peptide from avian smooth 
                     muscle myosin light chain kinase"
                     /label=calmodulin-binding peptide
                     /translation="RRKWNKTGHAVRAIGRLSS"
     primer_bind     complement(922..941)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     CDS             1693..1746
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
ORIGIN
        1 atgggaggag gcggctcagg aagtggagag ggatcaacta agatgcgtcg ggtagcaact
       61 ctcgtggacc tgggtacggg cggctcagag ctggacggtg gcctggagag cggacttcga
      121 agtggcagtg gcggcggcga cattatgatg ggaagtcatc atcatcatca tcatggaatg
      181 gccagtatga ctggtggcca gcaaatgggg agagatctgt acgacgatga cgataaggat
      241 ctggccacaa tggtggacag ttcacgccga aagtggaata agacaggcca cgcagtccgc
      301 gcaataggtc ggctgagtag cttggagaac gtttatatca aggccgacaa gcagaagaac
      361 ggcatcaagg cgaacttcaa gatccgccac aacatcgagg acggcggagt gcagctcgct
      421 taccactacc agcagaacac cccaattggc gacgggcccg tgctccttcc tgacaaccac
      481 tacctgtctg tacagtcaaa actgtcaaaa gacccaaacg aaaagcgaga tcacatggta
      541 ctcctcgagt tcgtcacagc agccgggatc acactcggta tggacgagct gtacaagggt
      601 gggactggcg ggagcatggt cagtaagggc gaggagctgt tcaccggcgt ggtgccgatt
      661 ctcgtcgagt tagacggtga cgtcaacggt cacaagttca gcgtgtcagg agagggcgag
      721 ggcgatgcaa cttacggtaa acttacactc aagttcatct gcaccaccgg aaagctgccc
      781 gtcccttggc ccactttagt gacgacactg acttacggcg tgcagtgctt ctctcgttac
      841 ccagaccaca tgaagcagca tgacttcttc aagtccgcca tgcccgaagg atacatccag
      901 gagagaacaa tcttcttcaa agacgacggc aactacaaga ctcgagcaga ggtgaagttc
      961 gaaggcgaca cactggtaaa ccggatcgag ctgaagggca ttgacttcaa agaggacggt
     1021 aacatactgg ggcacaagct cgagtacaac cttcctgacc aactgactga agagcagatc
     1081 gccgaattta aagaggaatt cagccttttc gacaaggacg gcgatgggac cataacgacc
     1141 aaggagttgg gaacggtcat gcggtctctg ggtcagaacc ctacagaagc agagcttcag
     1201 gacatgatta atgaagtgga tgcggacggt gacggcacta tagacttccc tgagttcctg
     1261 acaatgatgg ctcgtaaaat gaaataccgg gacacggaag aggaaataag agaagccttc
     1321 ggtgttttcg ataaggatgg caatgggtac atatcagctg cagagcttag gcacgtgatg
     1381 actaacctgg gtgaaaagtt gaccgatgaa gaggttgatg aaatgatcag ggaagccgac
     1441 atcgatgggg atggacaggt aaactacgaa gagttcgtgc aaatgatgac tgcgaagggt
     1501 tctggcagcg cgggtgggag tgcaggcgga tctgcaggcg gttcagctgg cgggtccgca
     1561 ggtggctccg caggcggcag cgccggtggc agccggggcg aaattgctaa gagcttaaag
     1621 gagattgcta agagcctgaa ggagatcgcc tggtctttga aggagatcgc caagagcctg
     1681 aagggcggaa ccgagggtag agggtcactc ctgacatgcg gcgacgttga agagaaccca
     1741 gggcccaagt tagcaaccat gcacaagttc agccaggagc agattggcga gaacatcgtg
     1801 tgcagggtca tatgcacaac aggccagatc ccgattaggg acctcagcgc cgacatatct
     1861 caggtgctga aggagaaacg ctctattaag aaagtgtgga cattcgggcg gaaccccgca
     1921 tgcgactacc atctgggcaa cattagcagg ttgtctaaca agcacttcca gattctgctg
     1981 ggcgaggacg gcaacctgtt gcttaacgac atcagcacca acggaacttg gttgaacgga
     2041 caaaaggtgg aaaagaactc taaccagttg ttgtcacagg gcgacgagat taccgttggg
     2101 gttggggtag agtcagatat cctcagctta gtcatattta ttaacgacaa gttcaagcag
     2161 tgtctggagc aaaacaaggt tgaccggggc tcaggctctg ccggcggaag tgcgggtggc
     2221 tccgccggtg gatcagccgg cggaagcgcg ggcggatcag caggcgggag tgcgggcggc
     2281 tctcgaacac tcagagaaat tgaagagctg ctccgaaaga tcatcgagga cagtgtacgc
     2341 tcagtggccg agctcgagga catcgagaaa tggttgaaga agatatga
//
