LOCUS       sequence-443348-       12397 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  CIRTS RNA targeting system for targeted knockdown of m6A-containing 
            Malat1 at the synapse. CIRTS-YTHDF2-Calm3 and Malat1 gRNA is 
            expressed under human Syn1 and U6 promoter, respectively..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12397)
  AUTHORS   Madugalle SU, Liau WS, Zhao Q, Li X, Gong H, Marshall PR, 
            Periyakaruppiah A, Zajaczkowski EL, Leighton LJ, Ren H, Musgrove 
            MRB, Davies JWA, Kim G, Rauch S, He C, Dickinson BC, Fulopova B, 
            Fletcher LN, Williams SR, Spitale RC, Bredy TW
  TITLE     Synapse-Enriched m(6)A-Modified Malat1 Interacts with the Novel 
            m(6)A Reader, DPYSL2, and Is Required for Fear-Extinction Memory.
  JOURNAL   J Neurosci. 2023 Oct 25;43(43):7084-7100. doi: 
            10.1523/JNEUROSCI.0943-23.2023. Epub 2023 Sep 5.
  PUBMED    37669863
REFERENCE   2  (bases 1 to 12397)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1523/JNEUROSCI.0943-23.2023"; journalName: "J Neurosci"; date: 
            "2023-10-25- 25"; volume: "43"; issue: "43"; pages: "7084-7100"
COMMENT     Sequence Label: CIRTS-YTHDF2-Malat1
FEATURES             Location/Qualifiers
     source          1..12397
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(47..66)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        238..617
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        619..817
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     772..792
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             835..1015
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1062..1187
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1680..1913
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2098..2142
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2440..2557
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     regulatory      3726..3735
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(3732..3734,3735..3737,3738..4448)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(3732..3734,3735..3737,3738..4448)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3777..3798)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4038..4057)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4385..4406
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             4458..4511
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             5028..5054
                     /codon_start=1
                     /product="nuclear export signal from the HIV Rev protein 
                     (Fischer et al., 1995)"
                     /label=NES
                     /translation="LPPLERLTL"
     CDS             5688..5711
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     promoter        6745..6993
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     6745..6765
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     6916..6935
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_feature    7089..7677
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7142..7162)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7560..7571)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(7680..7696)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7681..7697)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             8202..8382
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(8408..8425)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    8414..8638
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      8684..9112
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8771..8790)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8981..9002
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(9121..9141)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        9126..9455
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      9306..9441
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     9368..9387
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        9503..9550
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             9569..9943
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    10073..10194
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10110..10129)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10164..10183
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(10243..10259)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10243..10259)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(10256..10278)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    10267..10283
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(10291..10297,10298..10315,10316..10321))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10336..10357
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(10474..10491)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10645..11233)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10725..10744)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(11404..12195,12196..12264))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     12027..12046
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(12265..12369)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
       61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
      121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
      181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac
      241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
      301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
      361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
      421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
      481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
      541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
      601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
      661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
      721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
      781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct
      841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
      901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
      961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
     1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
     1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
     1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
     1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
     1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
     1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
     1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
     1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
     1501 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg
     1561 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga
     1621 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata
     1681 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg
     1741 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg
     1801 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag
     1861 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt
     1921 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt
     1981 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt
     2041 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag
     2101 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata
     2161 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta
     2221 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta
     2281 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa
     2341 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc ggcactgcgt
     2401 gcgccaattc tgcagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat
     2461 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa
     2521 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag
     2581 agatccagtt tggttaatta agggttttgg ctacgtccag agcagaggaa tgagggcatg
     2641 tagactaaat atgttcgtgt ggaagaggct gaatacacat cagagttagt gctgcaggaa
     2701 atgcttctgc attgcatacc cagagtttcc ttgctcatct gagagcatgt gttttttcca
     2761 gatgtgtgta cttgtgtgag attctctggg tgtgtgtcaa tgtgttgcct gaacgtgcat
     2821 tgctcaatat gctcatgtgt gttaccctgg gcttgtacat ctacatatat acctggatgc
     2881 ccgtgtgttc tgtgatgtac atataccctg tgtcattcct tgtttttcta tttgtgttat
     2941 tccatgtgtt ccttcaggct ctcactaccc aagtgtccac ctccgcctgt ctggtgatgt
     3001 ttacgctacc ccgtgctctt ttctttgcct gacagtgttg tcgtggaaga catctcgcca
     3061 ggaacactgc agtaaggaga atttctagtt ttatgttccc ctccgagtat gcttctatcc
     3121 cgaccctcaa ccccaaaatg ccttcagagg tgaaaatcaa cactggaaac acaagtatct
     3181 gggaagggta acaatgcaag ttagcctgag gatttaggag gaggctgaaa aacagagtag
     3241 gagccttact acgggtccag accctacgga caagaacccc cactcccact ccccaaattg
     3301 cgcattccct cccccatcag agggggaggg gaagaggatg cagcgcggcg cggcgcgtgc
     3361 gcactgtcgg atttagtacc gcggacagag ccttcgcccc cgctgccggc gcgcgccacc
     3421 acctccccag caccaaaggc gggctgacgt cactctccag ccctccccaa actcccctac
     3481 ctcaccgcct tggtcgcgtc cgtgcagcgg tgagtccagt cgggccgcac cacaagaggt
     3541 gcaagatagg ggggtgcagg cgcgaccata cgctctgcgg cggcagagcc tcagcgctgc
     3601 ctcagtctgc agcgggcagc agaggagtcg cgtcgtgcca gagagcgccg ccgtgctcct
     3661 gagccccttg cgctccgccc ccgcggccca ccgacccact gccccttgga tccccgggta
     3721 ccggtgccac catggtgagc aagggcgagg agctgttcac cggggtggtg cccatcctgg
     3781 tcgagctgga cggcgacgta aacggccaca agttcagcgt gtccggcgag ggcgagggcg
     3841 atgccaccta cggcaagctg accctgaagt tcatctgcac caccggcaag ctgcccgtgc
     3901 cctggcccac cctcgtgacc accctgacct acggcgtgca gtgcttcagc cgctaccccg
     3961 accacatgaa gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc
     4021 gcaccatctt cttcaaggac gacggcaact acaagacccg cgccgaggtg aagttcgagg
     4081 gcgacaccct ggtgaaccgc atcgagctga agggcatcga cttcaaggag gacggcaaca
     4141 tcctggggca caagctggag tacaactaca acagccacaa cgtctatatc atggccgaca
     4201 agcagaagaa cggcatcaag gtgaacttca agatccgcca caacatcgag gacggcagcg
     4261 tgcagctcgc cgaccactac cagcagaaca cccccatcgg cgacggcccc gtgctgctgc
     4321 ccgacaacca ctacctgagc acccagtccg ccctgagcaa agaccccaac gagaagcgcg
     4381 atcacatggt cctgctggag ttcgtgaccg ccgccgggat cactctcggc atggacgagc
     4441 tgtacaaggg ctccggcgag ggcaggggaa gtctactaac atgcggggac gtggaggaaa
     4501 atcccggccc aaccggtatg ggaattatca acacccttca aaaatactac tgtcgcgtcc
     4561 gtgggggtcg ttgcgcagtc ctgtcgtgcc tgcctaaaga ggagcagatc ggtaaatgct
     4621 caacccgtgg acgtaagtgc tgtcgtcgca agaaaggcgg ttctggaggc tcagggggga
     4681 gcggagggtc cggtggatca ggaggctcca tggcagttcc cgagacccgc cctaaccaca
     4741 ctatttatat caacaacctc aatagcaaga tcaagaagga tgagctaaaa aagtccctgt
     4801 acgccatctt ctcccagttt ggccagatcc tggatatcct ggtaccgcgg cagcgtaccc
     4861 cgaggggcca ggcctttgtc atcttcaagg aggtcagcag cgccaccaac gccctgcgct
     4921 ccatgcaggg tttccctttc tatgacaaac ctatgcgtat ccagtatgcc aagaccgaca
     4981 aacgtatccc ggccaagatg aaaggcacct tcgtgggatc ccttcaactg cctccacttg
     5041 aaagactgac actgggcggt agtggaggaa gcggggggag cggagggagt ggtggatccg
     5101 gcggctccat gtcggccagc agcctcttgg agcagagacc aaaaggtcaa ggaaacaaag
     5161 tacaaaatgg atctgtacat caaaaggatg gattaaacga tgatgatttt gaaccttact
     5221 tgagtccaca ggcaaggccc aataatgcat atactgccat gtcagattcc tacttaccca
     5281 gttactacag tccctccatt ggcttctcct attctttggg tgaagctgct tggtctacgg
     5341 ggggtgacac agccatgccc tacttaactt cttatggaca gctgagcaac ggagagcccc
     5401 acttcctacc agatgcaatg tttgggcaac caggagccct aggtagcact ccatttcttg
     5461 gtcagcatgg ttttaatttc tttcccagtg ggattgactt ctcagcatgg ggaaataaca
     5521 gttctcaggg acagtctact cagagctctg gatatagtag caattatgct tatgcaccta
     5581 gctccttagg tggagccatg attgatggac agtcagcttt tgccaatgag accctcaata
     5641 aggctcctgg catgaatact atagaccaag ggatggcagc aggatccgat tacaaggacg
     5701 acgatgacaa gtaggctagc gggagcctct gccctcctcc tgcagcccgg atggctctcc
     5761 tctgtttttg gtttgcttcc ttttgtttgc ccttcttttg ggtgaccggg gtggccacca
     5821 ttcctgtccc tgcccagtgg ggaggggagc aaggccttct gccaagagga ggagcatgtc
     5881 ccctgccact gttgcatgca ccccggccct gtgactctgt gtgcaagccc agcagcctgt
     5941 ggggcagggt gccaagaggg gacgggacgg acgttccagg acagagggag tcgaggatgt
     6001 gatccaggac gggtgggggg tggaagggtg ggaaagggca ggaaggcctt ggaagctaac
     6061 atgctttcac tactggaggg ggacggatag agcggatggc actcgggtct cactgacgct
     6121 gttctgaaac catctggctg gctttctgag gtcaggctgg gtagggggct gtcatcggcc
     6181 atactgctga tcacacttgc cctcatccgt ccagcgatct cacgctgttc tgtaaatacc
     6241 tggtgctaac atcccatgcc gctcccccat ggcaccccca gcctcctgcc cagttcaggc
     6301 tggtccagga atggatgtgg gggatggtcg ctgtgtaacg tgctggtgat ctttttcccc
     6361 tacccctttt atgaccctta aaactttgat tttgtcctaa acatgccggg ccaactaaag
     6421 ggtggggaga ggagagacga gccccaccat gctcacaaga accaacctgc aataaacact
     6481 tctgtgggcc gccgcgcaga gcacgcagcg ggcacctcct gcccctccat ggcgacttgg
     6541 catcgctttc tgcccgcctc tgccctcgcc ctgcctgccc caccccccag cggagagcat
     6601 gatccgcgcc cttgcttctg actctagcct ctgggacaca tcagtcaacg tgggcagttc
     6661 agtctgggtt cgttcctttc tctgttctca tctggccccc cagggcgatc ttgttgcttt
     6721 ttgctgggac ctgcccagtc tagagagggc ctatttccca tgattccttc atatttgcat
     6781 atacgataca aggctgttag agagataatt ggaattaatt tgactgtaaa cacaaagata
     6841 ttagtacaaa atacgtgacg tagaaagtaa taatttcttg ggtagtttgc agttttaaaa
     6901 ttatgtttta aaatggacta tcatatgctt accgtaactt gaaagtattt cgatttcttg
     6961 gctttatata tcttgtggaa aggacgaaac accggccaga tctgagcctg ggagctctct
     7021 ggccttattc caaatactag cctaaccttt ttgttttttt ttttgaattc gatatcaagc
     7081 ttatcgataa tcaacctctg gattacaaaa tttgtgaaag attgactggt attcttaact
     7141 atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgctattg
     7201 cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg tctctttatg
     7261 aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa
     7321 cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact ttcgctttcc
     7381 ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc tggacagggg
     7441 ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaaatcatcg tcctttcctt
     7501 ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac gtccttctgc tacgtccctt
     7561 cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg cggcctcttc
     7621 cgcgtcttcg ccttcgccct cagacgagtc ggatctccct ttgggccgcc tccccgcatc
     7681 gataccgtcg acctcgagac ctagaaaaac atggagcaat cacaagtagc aatacagcag
     7741 ctaccaatgc tgattgtgcc tggctagaag cacaagagga ggaggaggtg ggttttccag
     7801 tcacacctca ggtaccttta agaccaatga cttacaaggc agctgtagat cttagccact
     7861 ttttaaaaga aaagggggga ctggaagggc taattcactc ccaacgaaga caagatatcc
     7921 ttgatctgtg gatctaccac acacaaggct acttccctga ttggcagaac tacacaccag
     7981 ggccagggat cagatatcca ctgacctttg gatggtgcta caagctagta ccagttgagc
     8041 aagagaaggt agaagaagcc aatgaaggag agaacacccg cttgttacac cctgtgagcc
     8101 tgcatgggat ggatgacccg gagagagaag tattagagtg gaggtttgac agccgcctag
     8161 catttcatca catggcccga gagctgcatc cggactgtac tgggtctctc tggttagacc
     8221 agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa
     8281 gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga
     8341 gatccctcag acccttttag tcagtgtgga aaatctctag cagggcccgt ttaaacccgc
     8401 tgatcagcct cgactgtgcc ttctagttgc cagccatctg ttgtttgccc ctcccccgtg
     8461 ccttccttga ccctggaagg tgccactccc actgtccttt cctaataaaa tgaggaaatt
     8521 gcatcgcatt gtctgagtag gtgtcattct attctggggg gtggggtggg gcaggacagc
     8581 aagggggagg attgggaaga caatagcagg catgctgggg atgcggtggg ctctatggct
     8641 tctgaggcgg aaagaaccag ctggggctct agggggtatc cccacgcgcc ctgtagcggc
     8701 gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc
     8761 ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc
     8821 cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc
     8881 gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg
     8941 gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact
     9001 ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt
     9061 tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttaattctgt
     9121 ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc
     9181 aaagcatgca tctcaattag tcagcaacca ggtgtggaaa gtccccaggc tccccagcag
     9241 gcagaagtat gcaaagcatg catctcaatt agtcagcaac catagtcccg cccctaactc
     9301 cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa
     9361 ttttttttat ttatgcagag gccgaggccg cctctgcctc tgagctattc cagaagtagt
     9421 gaggaggctt ttttggaggc ctaggctttt gcaaaaagct cccgggagct tgtatatcca
     9481 ttttcggatc tgatcagcac gtgttgacaa ttaatcatcg gcatagtata tcggcatagt
     9541 ataatacgac aaggtgagga actaaaccat ggccaagttg accagtgccg ttccggtgct
     9601 caccgcgcgc gacgtcgccg gagcggtcga gttctggacc gaccggctcg ggttctcccg
     9661 ggacttcgtg gaggacgact tcgccggtgt ggtccgggac gacgtgaccc tgttcatcag
     9721 cgcggtccag gaccaggtgg tgccggacaa caccctggcc tgggtgtggg tgcgcggcct
     9781 ggacgagctg tacgccgagt ggtcggaggt cgtgtccacg aacttccggg acgcctccgg
     9841 gccggccatg accgagatcg gcgagcagcc gtgggggcgg gagttcgccc tgcgcgaccc
     9901 ggccggcaac tgcgtgcact tcgtggccga ggagcaggac tgacacgtgc tacgagattt
     9961 cgattccacc gccgccttct atgaaaggtt gggcttcgga atcgttttcc gggacgccgg
    10021 ctggatgatc ctccagcgcg gggatctcat gctggagttc ttcgcccacc ccaacttgtt
    10081 tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc
    10141 atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt
    10201 ctgtataccg tcgacctcta gctagagctt ggcgtaatca tggtcatagc tgtttcctgt
    10261 gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa
    10321 agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc
    10381 tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag
    10441 aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt
    10501 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga
    10561 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg
    10621 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa
    10681 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt
    10741 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct
    10801 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct
    10861 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc
    10921 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt
    10981 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc
    11041 tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat
    11101 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa
    11161 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa
    11221 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga
    11281 aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct
    11341 tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga
    11401 cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc
    11461 catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg
    11521 ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat
    11581 aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat
    11641 ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg
    11701 caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc
    11761 attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa
    11821 agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc
    11881 actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt
    11941 ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag
    12001 ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt
    12061 gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag
    12121 atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac
    12181 cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc
    12241 gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca
    12301 gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg
    12361 ggttccgcgc acatttcccc gaaaagtgcc acctgac
//
