LOCUS       sequence-443351-       12797 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express m-Cherry gene in mesenchymal lineage of Zebrafish..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12797)
  AUTHORS   Ali RH, Orellana EA, Lee SH, Chae YC, Chen Y, Clauwaert J, Kennedy 
            AL, Gutierrez AE, Papke DJ, Valenzuela M, Silverman B, Falzetta A, 
            Ficarro SB, Marto JA, Fletcher CDM, Perez-Atayde A, Alcindor T, 
            Shimamura A, Prensner JR, Gregory RI, Gutierrez A
  TITLE     A methyltransferase-independent role for METTL1 in tRNA 
            aminoacylation and oncogenic transformation.
  JOURNAL   Mol Cell. 2025 Mar 6;85(5):948-961.e11. doi: 
            10.1016/j.molcel.2025.01.003. Epub 2025 Jan 31.
  PUBMED    39892392
REFERENCE   2  (bases 1 to 12797)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2025.01.003"; journalName: "Mol Cell"; date: 
            "2025-03-6- 6"; volume: "85"; issue: "5"; pages: "948-961.e11"
COMMENT     Sequence Label: rag2:mcherry_CE_pISceI
FEATURES             Location/Qualifiers
     source          1..12797
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      5019..5028
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             5025..5735
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(5170..5188)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     5438..5457
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     polyA_signal    5745..5879
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             join(6946..6948,6949..6951,6952..7665)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(6946..6948,6949..6951,6952..7662)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(6991..7012)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(7252..7271)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     7599..7620
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    7967..8101
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8329..8345)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8329..8345)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     promoter        complement(8400..8418)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8428..8445)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8428..8444)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8437..8459)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      complement(8585..9040)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8722..8743)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     8934..8953
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        9067..9171
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9172..9240,9241..10032)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9390..9409)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10203..10791
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10692..10711
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10945..10962
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    11079..11100
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(11115..11120,11121..11138,11139..11145)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11153..11169
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     11158..11180
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     11177..11193
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     11177..11193
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     11212..11232
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        11214..11232
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 cctgtggtct gtgtgggtcc taatgcccca gtataaacca gcaggtctac ctgtggtctg
       61 tgtgggtctg tggttggttt ysddsttard gacsgcrtah vaggyagcmg ktcgwcycgt
      121 gggttngtag tggtgyctaa grsmcargta taaaccagca ggtcaacstg tggtctgtgt
      181 gggtcctaat gccccagtat aaaccagcag gtcaacctgt ggtctgtgtg ggtcctaatg
      241 ccccgngtay aaaccagcak gtcaacctgt ggtctgtgtg ggtcctaatg ccccagtata
      301 aaccagcagg tcaacctgtg gtctgtgtgg gtcctaatgc cccagtataa accagcaggt
      361 ctacctgtgg tctgtgtggg tcctaatgcc ccagtataaa ccagcaggtc aacctgtggt
      421 ctgtgtgggt yctaatgccc cagtataaac cagcaggtca acctgtggtc tgtgtgggtc
      481 ctaatgcccc agtataaacc agcaggtcaa cctgtggtct gtgtgggtcc taatgcccca
      541 gtataaacca gcagatcaac ctgtggtctg tgtgggtcct aaagtcccag gnataaacca
      601 gcatgtcaac ctgtggtctg tgtgggtcct aatgccccgg tataaaccag cagatcaacc
      661 tgtggtctgt gtgggtccta atgccccagg ataaaccagc aggtcaacct gtggtctgtg
      721 tgggtcctaa tgccccagta taaaccagca ggtcaacctg tggtctgtgt gggtcctaat
      781 gccccagtat attgatggag actctatact gctcggtgag ctccatcttt cggataagat
      841 gttaaaacaa ggtcccgact ctctgtggtg gtaaaaaatt cccaggatgt ccttcctacc
      901 ctggcatcct ggacaaattt gctcactgtc ctctgtccat catggcctcc taaccatccc
      961 catatcataa ttggctttat cactctagct ggggccgaaa tcgcatactt ccattttatg
     1021 aggtacgcta aaatcagtat gcgagccaag taatatgtcc gaatacttcc ggcgagattc
     1081 tagagtgtgc attcgatgca cgctacgcta ttccatgatg ccccgtgaga gaattcttga
     1141 atcggagaga agcaacgcaa ctcatgcggg taggttacgt gacgatgaca tattggcgga
     1201 tgtagtacgt ccaagttcca ttcatactgc tcacattcat attttataga atgttctttt
     1261 cccacagcca agtagtaagt ttaaattcaa atacagtacc tactaagtag tagtagacgg
     1321 tttcggacgc agcccctgtc tcctctccac caatatactg gtgtgtagtg tgcggtctgg
     1381 tgcaatatgg ctgccatcgc gtcatccagg tggatgctgc acactggtgg ttgatgagga
     1441 gattttgccc aaaaaagtgt tttgagtttc cagaaaagcg cgatataaat gtaaggaatt
     1501 atcattagct tattataatg gaactttctg gataacttgt gccagagcta ggggccatta
     1561 aaactttgcc ctggggtacc tcagggagct gcaaggaagt gctatagggc taatgaaatg
     1621 tttgggggaa aaaaaaaaat tttataagta aaaataaaac taaaataatt aataatttca
     1681 ttgttgaaaa ttatagtaaa attgataaac aagataagca gcattagtat aaaaaactaa
     1741 aaacaaacgt aatttaaaat aattaaattg ttttaaataa attatacaaa ataatgttta
     1801 caacgacacg gtggtgcagt aggtagtgct gtcgccagca aagcaagaag gtcgctggtt
     1861 cgagcctcag ctggatcact tgatgtttct gtgtggagca ttcgcgtgcg tttaacccca
     1921 gattaataaa gagactaagc cgaaaagaaa ataaatgtat gaaaatgttt acgtaaaatg
     1981 taaataaatg catgattaat aaaatatatg aattttaaag attgaggtag aattgtttta
     2041 ttattcacac atttggttat ttttaatcag tgacaacttg tgatgctctt tatattgttt
     2101 attacgctac tttgaatatt cagtctcaaa ttgcatgtaa atacgacttc aaagcagaat
     2161 tagtttaatt taattgcctg taaacagtgt tgtactttga tggtgattgt ctgataataa
     2221 tatgatttca ctatttaaaa tttgaaaaaa aaaaatctga aaaatactca ccggccactt
     2281 tattaggtac acctgtctaa ctgcttgtta acgtaaattt ctaatcagcc aatcaccagg
     2341 cagcaactca gtgcatttag gcatgtagac atggtcaaga cgatctgctg cagttcaaag
     2401 cgagcatcag aatggagaag caaggtgatt taagtgactt taaacgtgac gtgtttgttg
     2461 gtgccagacg ggctggtctg agtatttcag aaactgctga tttactgggg ttttcacaca
     2521 caaacatctc tggggtttac agagaatggt ccaaaaaaag ataatatcca gtgagcggca
     2581 gttctgtggg ggcaaatgtc ttgttgatgc cagaggtcag aggagaatgg cctgactggt
     2641 tccagctgat agaaaggcaa cagtaattca aataaccact cgttagaact gaggcatgca
     2701 gaagagcacc tttgaacaca acatgtcgaa ccttgaggcg ggtgggctac agcagctcaa
     2761 gaccacatcg ggtgccactc ctgtcagcta agaacaggaa actgaggcta caattcgcac
     2821 aggctcaaca taagtagaca atagaagatt gaacattatg ttgcctggtc agatggatac
     2881 attctacctt gtatcaacag ttcaggctgg tgatggtggt gtaatggtgt gggggatata
     2941 ttcttggcac actttgggcc cattagtacc aattaaccat cgtgtcaacg ccacagccta
     3001 actgagtatt gttgctgacc atgttcattt ctttttgacc acagtgtacc catcttctga
     3061 tggctacttc ctgcaggata atgcaccatg tcataaagcg tgaatgatct cagactggtt
     3121 tcttgaacat gacaatgagt tcactgtgct cgaatgacct ccacagtcac cagatctcaa
     3181 tccaatagag cgcctttggg gtgtggtaga atggaagatt cgcatcctgg atgtgcagcc
     3241 gacaaatctg catgatgcta gcatgtcaat atggaccaaa atctctgagg aattttccag
     3301 tacctggttg aaatgcaaaa ggaagggaat ctatgctaag gcaaaaagag gtccaaccca
     3361 gtactagtgg ggtgtacata ataaagtggc cggtgagtgt atattgttaa ggatatctag
     3421 ataatccaaa taatttacag cactatttaa aaaaaaaact ttatgtcacc aagtctgcat
     3481 ttatttgatc acaattacag tcgaaaaagc aatatcggga tacattgttt ccatttaaaa
     3541 taactatatt atattttaaa tatttttgac aatgttattt ctttaatgcc aaagctaaat
     3601 tgtattcaat ctgaatttta gtgtcacatg atccttcaga aaatattaac acattgctaa
     3661 aaatgtatat tattatcaat ttggantttt tttttttttt ttgtatatta acctatgcta
     3721 ttggttacta tacaaaagta ttactgtgaa tatatgcata tacagaaccg tatcaagcgc
     3781 ggggggtggt tgggttatgt ccccatttgg aaagacctgg aaacattcat aatgactaaa
     3841 attgcacgga aaaatataat atctatgagt attgctgatc agcagcacaa aaccagaact
     3901 ccatattaat aaacagccaa tgatgcagtg agtttgtgca tgttttcatt caaactcgtg
     3961 tgattgtgct caaacacctg ttgtgagcag cagttgcaaa cagtaaacct caagaggagc
     4021 cagtcaacct tcacaaacac tcaacctctg gctcactcac taaacaattc gcgttaatat
     4081 ctaaaataaa aatttccagc accaaacagc tctcagattt cggagtacac tgaccaagag
     4141 gtaagaagta acgttacatt actaggacga ctattgtaag actagatatg taatatttgg
     4201 aagcgtacgt gttttaagtg acatatagag tagaggaact gatggaaata cgatttgcat
     4261 aatgtaaaat gatatatatt aaaatatagg ctacagtagg taacgtggtt tacatataac
     4321 gttactgtgt taatgtagtg ctcagttact cgtgttgaca gttattatta gtgcttgcta
     4381 atttgtttgt taatgcttct cttgcgtatt tgattacctt caggcgaatc taaaacatat
     4441 cagatgtaga aacggcagaa aaatgttgaa gaaaatacga gaatagtagc ttttggagca
     4501 catagaaaag tacatcagat aaagcctacc gtttaaacgc agacgccgct ctcgcactga
     4561 atgctgggta ctgtagtcct tatattgtat gcttaatgtg cgtctcaacg ggaaactaga
     4621 gttaggaaaa ctacaatttc ctgtggaaaa tgtttctgtt ttcggggaaa cagcgaaaga
     4681 aagatatggt tccgcgtttt tttcttactt tgaataataa aataagacct aactacacac
     4741 ttcgcttact cttatgctaa tttgaagcac tagcattctt tgatacaatg ataatcattt
     4801 aaacatcaca gttatactac aaatactatg gttaaactat gtctagtgta gtgtaacact
     4861 atggttgcca ttgttatctt taagtttaag tattatctgt ggtaaagcca tggttggttt
     4921 ttataagagg taataaaatt aacattgtac aattaaatga ctataaatta atgttttttt
     4981 ttattttttt tacacagcta ccttcaaaaa gggatcccgc caccatggtg agcaagggcg
     5041 aggaggataa catggccatc atcaaggagt tcatgcgctt caaggtgcac atggagggct
     5101 ccgtgaacgg ccacgagttc gagatcgagg gcgagggcga gggccgcccc tacgagggca
     5161 cccagaccgc caagctgaag gtgaccaagg gtggccccct gcccttcgcc tgggacatcc
     5221 tgtcccctca gttcatgtac ggctccaagg cctacgtgaa gcaccccgcc gacatccccg
     5281 actacttgaa gctgtccttc cccgagggct tcaagtggga gcgcgtgatg aacttcgagg
     5341 acggcggcgt ggtgaccgtg acccaggact cctccctgca ggacggcgag ttcatctaca
     5401 aggtgaagct gcgcggcacc aacttcccct ccgacggccc cgtaatgcag aagaagacca
     5461 tgggctggga ggcctcctcc gagcggatgt accccgagga cggcgccctg aagggcgaga
     5521 tcaagcagag gctgaagctg aaggacggcg gccactacga cgctgaggtc aagaccacct
     5581 acaaggccaa gaagcccgtg cagctgcccg gcgcctacaa cgtcaacatc aagttggaca
     5641 tcacctccca caacgaggac tacaccatcg tggaacagta cgaacgcgcc gagggccgcc
     5701 actccaccgg cggcatggac gagctgtaca agtgaatcga tgatgatcca gacatgataa
     5761 gatacattga tgagtttgga caaaccacaa ctagaatgca gtgaaaaaaa tgctttattt
     5821 gtgaaatttg tgatgctatt gctttatttg taaccattat aagctgcaat aaacaagtta
     5881 acaacaacaa ttctgcacag caccttgacc tggaaattag ggaaattata acagtcaatc
     5941 agtggaagaa aatggaggaa gtatgtgatt catcagcagc tgcgagcagc acagtccaaa
     6001 atcagccaca ggatcaagag cacccgtggc cgtatcttcg cagatctaaa gcttaaatca
     6061 gttgtgttaa ataagagaca ttcaaaataa atgtaaatga gctctccaaa tcagcagact
     6121 taacattctt taaaatgatt gattcaataa tgataaaaat caggcatagc cagttgtaac
     6181 tttagataaa ttacagaaaa tgtcaaatac agagaaccga ttctttttta tgatacatcc
     6241 aagcacacat ttaacacaat ccaggcaaac cccgaatttc acagtcacaa gcactgtttg
     6301 tacaagagct ttgcctaagg acacacagtc tctataagtc caggtcgttg gtttcactct
     6361 tattttaaac atgtgacatt tttcctgcca tcctgtctta ggctgctgtt tgcttcattc
     6421 catgtcacat taaattcctc agtagcacct tttacacaca cagccaatct tttccagaaa
     6481 attcaattgc tttgaagaga taatgtgtga acaaatccat ttagaaaagg aaaattaaga
     6541 atttgtaaaa tcatctgtaa attgttggca ttcttctgta tatgaacatc acatcattta
     6601 caggtaaagg tctggtcatt aattatatga caatttactg gtattatttt gtgaaagggg
     6661 ctattttcaa tgcgttcatc catccttttc atccctcaaa tctctcattc acgtccccct
     6721 ccccatctgc acactttatc tcatttccac cctgctggaa tctgagcact tgtgcagtta
     6781 tcagggctcc tgtatttagg aggctctggg tgtccatgta ggggacgaac agaaacactg
     6841 cagaccttta tagaagaaca attgataaga gtcctcatac ataaagactc cattagtaag
     6901 ccagtgaccc aggagcccag accaacagca aagcagacag tgaccatggt gagcaagggc
     6961 gaagagctgt tcaccggggt ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc
     7021 cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa gctgaccctg
     7081 aagttcatct gcaccaccgg caagctgccc gtgccctggc ccaccctcgt gaccaccctg
     7141 acctacggcg tgcagtgctt cagccgctac cccgaccaca tgaagcagca cgacttcttc
     7201 aagtccgcca tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa ggacgacggc
     7261 aactacaaga cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag
     7321 ctgaagggca tcgacttcaa ggaggacggc aacatcctgg ggcacaagct ggagtacaac
     7381 tacaacagcc acaacgtcta tatcatggcc gacaagcaga agaacggcat caaggtgaac
     7441 ttcaagatcc gccacaacat cgaggacggc agcgtgcagc tcgccgacca ctaccagcag
     7501 aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca accactacct gagcacccag
     7561 tccgccctga gcaaagaccc caacgagaag cgcgatcaca tggtcctgct ggagttcgtg
     7621 accgccgccg ggatcactct cggcatggac gagctgtaca agtaaagcgg cccctctcga
     7681 gcctctagaa ctatagtgag tcgtattacg tagatccaga catgataaga tacattgatg
     7741 agtttggaca aaccacaact agaatgcagt gaaaaaaatg ctttatttgt gaaatttgtg
     7801 atgctattgc tttatttgta accattataa gctgcaataa acaagagatc tgatctagag
     7861 gatcataatc agccatacca catttgtaga ggttttactt gctttaaaaa acctcccaca
     7921 cctccccctg aacctgaaac ataaaatgaa tgcaattgtt gttgttaact tgtttattgc
     7981 agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt
     8041 ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctggat
     8101 catcatcgat gcgtcttttg ttctggtcat caaggagggg ggaatgttct gcgcatgcct
     8161 gtgggggagg gagaaggaca cgtcactgaa aacgtccctg catcacaccg agacacccaa
     8221 gaattgcatt cattttatgt ttcaggttca gggggaggtg tgggaggttt tttaaagcaa
     8281 gtaaaacctc tacaaatgtg gtatggctga ttatgatcct ctagatcaga tccactagtt
     8341 ctagagcggc cattaccctg ttatccctag gccgccaccg cggtggagct ccaattcgcc
     8401 ctatagtgag tcgtattacg cgcgctcact ggccgtcgtt ttacaacgtc gtgactggga
     8461 aaaccctggc gttacccaac ttaatcgcct tgcagcacat ccccctttcg ccagctggcg
     8521 taatagcgaa gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga
     8581 atggaaattg taagcgttaa tattttgtta aaattcgcgt taaatttttg ttaaatcagc
     8641 tcatttttta accaataggc cgaaatcggc aaaatccctt ataaatcaaa agaatagacc
     8701 gagatagggt tgagtgttgt tccagtttgg aacaagagtc cactattaaa gaacgtggac
     8761 tccaacgtca aagggcgaaa aaccgtctat cagggcgatg gcccactacg tgaaccatca
     8821 ccctaatcaa gttttttggg gtcgaggtgc cgtaaagcac taaatcggaa ccctaaaggg
     8881 agcccccgat ttagagcttg acggggaaag ccggcgaacg tggcgagaaa ggaagggaag
     8941 aaagcgaaag gagcgggcgc tagggcgctg gcaagtgtag cggtcacgct gcgcgtaacc
     9001 accacacccg ccgcgcttaa tgcgccgcta cagggcgcgt caggtggcac ttttcgggga
     9061 aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc
     9121 atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt
     9181 caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct
     9241 cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt
     9301 tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt
     9361 tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac
     9421 gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac
     9481 tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct
     9541 gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg
     9601 aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg
     9661 gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca
     9721 atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa
     9781 caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt
     9841 ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc
     9901 attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg
     9961 agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt
    10021 aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt
    10081 catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc
    10141 ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct
    10201 tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta
    10261 ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc
    10321 ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac
    10381 ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct
    10441 gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat
    10501 aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg
    10561 acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa
    10621 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg
    10681 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga
    10741 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc
    10801 aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct
    10861 gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct
    10921 cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgccca
    10981 atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg
    11041 tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta gctcactcat
    11101 taggcacccc aggctttaca ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc
    11161 ggataacaat ttcacacagg aaacagctat gaccatgatt acgccaagcg cgcaattaac
    11221 cctcactaaa gggaacaaaa gctgggtacc gggccccccc tcgaattacc ctgttatccc
    11281 tatcgagcaa aatcaatcct aatgtaaaat agtatacgtt catcattata atgcaaacac
    11341 tgtagaactg ttgtgttgtg ccttgcattt cattctcagt ttcttatcgt ctgtctcttt
    11401 ttacgacaga gctagaaaaa actagccttc atttgccatc gccgtatgca aatccactcc
    11461 agaactcaac aggtttgttc tgcggcgggt gtaagtgtga acaccggcat gtaagtgcgc
    11521 ttgagcagga gaataaagcg ctcagagaca ccggaggaat cctttatttt gcgttgcgcc
    11581 tcaggggatt cgctgagcct tctcttcatg gccctgctct tcagacacag gtgctccgct
    11641 gtttgtgttt ttcactgttc ttcaggcact cgtggttacg ggtttttgtt ttgcactctt
    11701 gcacacacat ctctcggcct ccaaaacaaa cacacaactg cacgagggct cgaagccccc
    11761 accgaaaaac aaaaacatta tgctaatgag gaaggcacag ctgttccctt cttcactctc
    11821 tctctcgttc tttcaacaac agtcgcctaa tttctctcgt tcctttaaag agccgccaag
    11881 cagaaacagt taatgcaaat ataagcctgc gggaaataag gagaattttt acaaaatccg
    11941 agcttcccct cctgccaaaa tgtgatgtgt gactcacgca ttcgatattt ggtgtaatga
    12001 gagtgaatca ttctgctcca caccgaaccc gatgccacag accactcaga tgcacaccga
    12061 ggagactatt ttaacagcac attacatcca gactgtcaca gatgcattac caagagtgtg
    12121 atttcagtgt cgaatgcagg tcttaaagga aaatctgttg cgattgcatg tgcgctaact
    12181 agacacgacc aacgaatggg aacaatctgt gaaatattat acaatcacag gcaattaaaa
    12241 gatgtttaaa gttcaattta gttttaatta atatgttata taaaactaat ctgttttaaa
    12301 tgtatattta tgattttagc tttagctagc ctcttgacca gcagctgaag tgtccggcaa
    12361 gtacctaaaa gggagaccac atgggaaagc taggttgctc ccggaagagg tgttagtaaa
    12421 ccagcaggtc tacctgtggt ctgtgtgggt cctaatgccc cagtataaac cagcaggtca
    12481 acctgtggtc tgtgtgggtc ctaatgcccc agtataaacc agcaggtcta cctgtggtct
    12541 gtgtgggtcc taatgcccca gtataaacca gcaggtcaac ctgtagtctg tgtgggtcct
    12601 aatgccccag tataaaccag caggtctacc tgtggtctgt gtgggtccta atgccccagt
    12661 ataaaccagc aggtcaacct gtgrtctgtg tgggtcctaa tgccccagta taaamcakca
    12721 ygtcamccts twygmtttct tkttsctwat ttyattgtta agactaattt gtcacactcg
    12781 tacacattag tatacgt
//
