LOCUS       sequence-443354-       12504 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express METTL1-S27A gene in mesenchymal lineage of Zebrafish..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12504)
  AUTHORS   Ali RH, Orellana EA, Lee SH, Chae YC, Chen Y, Clauwaert J, Kennedy 
            AL, Gutierrez AE, Papke DJ, Valenzuela M, Silverman B, Falzetta A, 
            Ficarro SB, Marto JA, Fletcher CDM, Perez-Atayde A, Alcindor T, 
            Shimamura A, Prensner JR, Gregory RI, Gutierrez A
  TITLE     A methyltransferase-independent role for METTL1 in tRNA 
            aminoacylation and oncogenic transformation.
  JOURNAL   Mol Cell. 2025 Mar 6;85(5):948-961.e11. doi: 
            10.1016/j.molcel.2025.01.003. Epub 2025 Jan 31.
  PUBMED    39892392
REFERENCE   2  (bases 1 to 12504)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2025.01.003"; journalName: "Mol Cell"; date: 
            "2025-03-6- 6"; volume: "85"; issue: "5"; pages: "948-961.e11"
COMMENT     Sequence Label: rag2:METTL1-S27A_CE_pISceI
FEATURES             Location/Qualifiers
     source          1..12504
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     gap             908..1007
                     /estimated_length=100
     polyA_signal    6899..6980
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(join(7282..7995,7996..7998,7999..8001))
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             complement(join(7285..7995,7996..7998,7999..8001))
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(7327..7348)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     7676..7695
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     7935..7956
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(9182..9198)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9182..9198)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     promoter        complement(9253..9271)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(9281..9298)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(9281..9297)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9290..9312)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      complement(9438..9893)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9575..9596)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     9787..9806
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        9920..10024
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(10025..10093,10094..10885)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10243..10262)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      11056..11644
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11545..11564
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     11798..11815
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    11932..11953
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(11968..11973,11974..11991,11992..11998)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12006..12022
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     12011..12033
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     12030..12046
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     12030..12046
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     12065..12085
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        12067..12085
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 tcactgttct tcaggcactc gtggttacgg gtttttgttt tgcactcttg cacacacatc
       61 tctcggcctc caaaacaaac acacaactgc acgagggctc gaagccccca ccgaaaaaca
      121 aaaacattat gctaatgagg aaggcacagc tgttcccttc ttcactctct ctctcgttct
      181 ttcaacaaca gtcgcctaat ttctctcgtt cctttaaaga gccgccaagc agaaacagtt
      241 aatgcaaata taagcctgcg ggaaataagg agaattttta caaaatccga gcttcccctc
      301 ctgccaaaat gtgatgtgtg actcacgcat tcgatatttg gtgtaatgag agtgaatcat
      361 tctgctccac accgaacccg atgccacaga ccactcagat gcacaccgag gagactattt
      421 taacagcaca ttacatccag actgtcacag atgcattacc aagagtgtga tttcagtgtc
      481 gaatgcaggt cttaaaggaa aatctgttgc gattgcatgt gcgctaacta gacacgacca
      541 acgaatggga acaatctgtg aaatattata caatcacagg caattaaaag atgtttaaag
      601 ttcaatttag ttttaattaa tatgttatat aaaactaatc tgttttaaat gtatatttat
      661 gattttagct ttagctagcc tcttgaccag cagctgaagt gtccggcaag tacctaaaag
      721 ggagaccaca tgggaaagct aggttgctcc cggaagaggt gttagtaaac cagcaggtct
      781 acctgtggtc tgtgtgggtc ctaatgcccc agtataaacc agcaggtcaa cctgtggtct
      841 gtgtgggtcc taatgcccca gtataaacca gcaggtctac ctgtggtctg tgtgggtcct
      901 aatgcccnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      961 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnncta cctgtggtct
     1021 gtgtgggtcc taatgcccca gtataaacca gcaggtcaac ctgtggtctg tgtgggtcct
     1081 aatgccccag tataaaccag caggtctacc tgtggtctgt gtgggtccta atgcaccagt
     1141 ataaaccagc aggtcaacct gtggtctgtg tgggttctaa tgccccagta taaaccagca
     1201 ggtcaacctg tggtctgtgt gggtccaaat gccccagtac aaaccagcag gtcaacctgt
     1261 ggtctgtgtg ggtcctaatg ccccagtata aaccagcagg tcaacctgtg gtctgtgtgg
     1321 gtcctaatgc cccggtacaa accagcatgt caacctgtgg tctgtgtggg tcctaatgcc
     1381 ccggtataaa ccagcagatc aacctgtggt ctgtgtgggt cctaatgccc cagtataaac
     1441 cagcagatca acctgtggtc tgtgtgggtc ctaaagtccc aggataaacc agcatgtcaa
     1501 cctgtggtct gtgtgggtcc taatgccccg gtataaacca gcagatcaac ctgtggtctg
     1561 tgtgggtcct aatgccccag gataaaccag caggtcaacc tgtggtctgt gtgggtccta
     1621 atgccccagt ataaaccagc aggtcaacct gtggtctgtg tgggtcctaa tgccccagta
     1681 tattgatgga gactctatac tgctcggtga gctccatctt tcggataaga tgttaaaaca
     1741 aggtcccgac tctctgtggt ggtaaaaaat tcccaggatg tccttcctac cctggcatcc
     1801 tggacaaatt tgctcactgt cctctgtcca tcatggcctc ctaaccatcc ccatatcata
     1861 attggcttta tcactctagc tggggccgaa atcgcatact tccattttat gaggtacgct
     1921 aaaatcagta tgcgagccaa gtaatatgtc cgaatacttc cggcgagatt ctagagtgtg
     1981 cattcgatgc acgctacgct attccatgat gccccgtgag agaattcttg aatcggagag
     2041 aagcaacgca actcatgcgg gtaggttacg tgacgatgac atattggcgg atgtagtacg
     2101 tccaagttcc attcatactg ctcacattca tattttatag aatgttcttt tcccacagcc
     2161 aagtagtaag tttaaattca aatacagtac ctactaagta gtagtagacg gtttcggacg
     2221 cagcccctgt ctcctctcca ccaatatact ggtgtgtagt gtgcggtctg gtgcaatatg
     2281 gctgccatcg cgtcatccag gtggatgctg cacactggtg gttgatgagg agattttgcc
     2341 caaaaaagtg ttttgagttt ccagaaaagc gcgatataaa tgtaaggaat tatcattagc
     2401 ttattataat ggaactttct ggataacttg tgccagagct aggggccatt aaaactttgc
     2461 cctggggtac ctcagggagc tgcaaggaag tgctataggg ctaatgaaat gtttggggga
     2521 aaaaaaaaaa ttttataagt aaaaataaaa ctaaaataat taataatttc attgttgaaa
     2581 attatagtaa aattgataaa caagataagc agcattagta taaaaaacta aaaacaaacg
     2641 taatttaaaa taattaaatt gttttaaata aattatacaa aataatgttt acaacgacac
     2701 ggtggtgcag taggtagtgc tgtcgccagc aaagcaagaa ggtcgctggt tcgagcctca
     2761 gctggatcac ttgatgtttc tgtgtggagc attcgcgtgc gtttaacccc agattaataa
     2821 agagactaag ccgaaaagaa aataaatgta tgaaaatgtt tacgtaaaat gtaaataaat
     2881 gcatgattaa taaaatatat gaattttaaa gattgaggta gaattgtttt attattcaca
     2941 catttggtta tttttaatca gtgacaactt gtgatgctct ttatattgtt tattacgcta
     3001 ctttgaatat tcagtctcaa attgcatgta aatacgactt caaagcagaa ttagtttaat
     3061 ttaattgcct gtaaacagtg ttgtactttg atggtgattg tctgataata atatgatttc
     3121 actatttaaa atttgaaaaa aaaaaatctg aaaaatactc accggccact ttattaggta
     3181 cacctgtcta actgcttgtt aacgtaaatt tctaatcagc caatcaccag gcagcaactc
     3241 agtgcattta ggcatgtaga catggtcaag acgatctgct gcagttcaaa gcgagcatca
     3301 gaatggagaa gcaaggtgat ttaagtgact ttaaacgtga cgtgtttgtt ggtgccagac
     3361 gggctggtct gagtatttca gaaactgctg atttactggg gttttcacac acaaacatct
     3421 ctggggttta cagagaatgg tccaaaaaaa gataatatcc agtgagcggc agttctgtgg
     3481 gggcaaatgt cttgttgatg ccagaggtca gaggagaatg gcctgactgg ttccagctga
     3541 tagaaaggca acagtaattc aaataaccac tcgttagaac tgaggcatgc agaagagcac
     3601 ctttgaacac aacatgtcga accttgaggc gggtgggcta cagcagctca agaccacatc
     3661 gggtgccact cctgtcagct aagaacagga aactgaggct acaattcgca caggctcaac
     3721 ataagtagac aatagaagat tgaacattat gttgcctggt cagatggata cattctacct
     3781 tgtatcaaca gttcaggctg gtgatggtgg tgtaatggtg tgggggatat attcttggca
     3841 cactttgggc ccattagtac caattaacca tcgtgtcaac gccacagcct aactgagtat
     3901 tgttgctgac catgttcatt tctttttgac cacagtgtac ccatcttctg atggctactt
     3961 cctgcaggat aatgcaccat gtcataaagc gtgaatgatc tcagactggt ttcttgaaca
     4021 tgacaatgag ttcactgtgc tcgaatgacc tccacagtca ccagatctca atccaataga
     4081 gcgcctttgg ggtgtggtag aatggaagat tcgcatcctg gatgtgcagc cgacaaatct
     4141 gcatgatgct agcatgtcaa tatggaccaa aatctctgag gaattttcca gtacctggtt
     4201 gaaatgcaaa aggaagggaa tctatgctaa ggcaaaaaga ggtccaaccc agtactagtg
     4261 gggtgtacat aataaagtgg ccggtgagtg tatattgtta aggatatcta gataatccaa
     4321 ataatttaca gcactattta aaaaaaaaac tttatgtcac caagtctgca tttatttgat
     4381 cacaattaca gtcgaaaaag caatatcggg atacattgtt tccatttaaa ataactatat
     4441 tatattttaa atatttttga caatgttatt tctttaatgc caaagctaaa ttgtattcaa
     4501 tctgaatttt agtgtcacat gatccttcag aaaatattaa cacattgcta aaaatgtata
     4561 ttattatcaa tttggatttt tttttttttt ttgtatatta acctatgcta ttggttacta
     4621 tacaaaagta ttactgtgaa tatatgcata tacagaaccg tatcaagcgc ggggggtggt
     4681 tgggttatgt ccccatttgg aaagacctgg aaacattcat aatgactaaa attgcacgga
     4741 aaaatataat atctatgagt attgctgatc agcagcacaa aaccagaact ccatattaat
     4801 aaacagccaa tgatgcagtg agtttgtgca tgttttcatt caaactcgtg tgattgtgct
     4861 caaacacctg ttgtgagcag cagttgcaaa cagtaaacct caagaggagc cagtcaacct
     4921 tcacaaacac tcaacctctg gctcactcac taaacaattc gcgttaatat ctaaaataaa
     4981 aatttccagc accaaacagc tctcagattt cggagtacac tgaccaagag gtaagaagta
     5041 acgttacatt actaggacga ctattgtaag actagatatg taatatttgg aagcgtacgt
     5101 gttttaagtg acatatagag tagaggaact gatggaaata cgatttgcat aatgtaaaat
     5161 gatatatatt aaaatatagg ctacagtagg taacgtggtt tacatataac gttactgtgt
     5221 taatgtagtg ctcagttact cgtgttgaca gttattatta gtgcttgcta atttgtttgt
     5281 taatgcttct cttgcgtatt tgattacctt caggcgaatc taaaacatat cagatgtaga
     5341 aacggcagaa aaatgttgaa gaaaatacga gaatagtagc ttttggagca catagaaaag
     5401 tacatcagat aaagcctacc gtttaaacgc agacgccgct ctcgcactga atgctgggta
     5461 ctgtagtcct tatattgtat gcttaatgtg cgtctcaacg ggaaactaga gttaggaaaa
     5521 ctacaatttc ctgtggaaaa tgtttctgtt ttcggggaaa cagcgaaaga aagatatggt
     5581 tccgcgtttt tttcttactt tgaataataa aataagacct aactacacac ttcgcttact
     5641 cttatgctaa tttgaagcac tagcattctt tgatacaatg ataatcattt aaacatcaca
     5701 gttatactac aaatactatg gttaaactat gtctagtgta gtgtaacact atggttgcca
     5761 ttgttatctt taagtttaag tattatctgt ggtaaagcca tggttggttt ttataagagg
     5821 taataaaatt aacattgtac aattaaatga ctataaatta atgttttttt ttattttttt
     5881 tacacagcta ccttcaaaaa gggatccatg gcagccgaga ctcggaacgt ggccggagca
     5941 gaggccccac cgccccagaa gcgctactac cggcaacgtg ctcacgcgaa ccccatggcg
     6001 gaccacacgc tgcgctaccc tgtgaagcca gaggagatgg actggtctga gctataccca
     6061 gagttcttcg ctccactcac tcaaaatcag agccacgatg acccaaagga taagaaagaa
     6121 aagagagctc aggcccaagt ggagtttgca gacataggct gtggctatgg tggcctgtta
     6181 gtggaactgt caccgctgtt cccagacaca cttattctgg gtctggagat ccgggtgaag
     6241 gtctcagact atgtacaaga ccggattcgg gccctacgcg cagctcctgc aggtggcttc
     6301 cagaacatcg cctgtctccg tagcaatgcc atgaagcacc ttcctaactt cttctacaag
     6361 ggccagctga caaagatgtt cttcctcttc cccgacccac atttcaagcg gacaaagcac
     6421 aagtggcgaa tcatcagtcc caccctgcta gcagaatatg cctacgtgct aagagttggg
     6481 gggctggtgt ataccataac cgatgtgctg gagctacacg actggatgtg cactcatttc
     6541 gaagagcacc cactgtttga gcgtgtgcct ctggaggacc tgagtgaaga ccccgttgtg
     6601 ggacatctag gcacctcaac tgaggagggg aagaaagttc tacgtaatgg agggaagaat
     6661 ttcccagcca tcttccgaag aatacaagat cccgtcctcc aggcagtgac ctcccaaacc
     6721 agcctgcctg gtcactgaat cgatgatgat ccagacatga taagatacat tgatgagttt
     6781 ggacaaacca caactagaat gcagtgaaaa aaatgcttta tttgtgaaat ttgtgatgct
     6841 attgctttat ttgtaaccat tataagattg atgagtttgg acaaaccaca actagaatgc
     6901 agtgaaaaaa atgctttatt tgtgaaattt gtgatgctat tgctttattt gtaaccatta
     6961 taagctgcaa taaacaagtt aacaacaaca attgcattca ttttatgttt caggttcagg
     7021 gggaggtgtg ggaggttttt taaagcaagt aaaacctcta caaatgtggt atggctgatt
     7081 atgatcctct agatcagatc tcttgtttat tgcagcttat aatggttaca aataaagcaa
     7141 tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc
     7201 caaactcatc aatgtatctt atcatgtctg gatctacgta atacgactca ctatagttct
     7261 agaggctcga gaggggccgc tttacttgta cagctcgtcc atgccgagag tgatcccggc
     7321 ggcggtcacg aactccagca ggaccatgtg atcgcgcttc tcgttggggt ctttgctcag
     7381 ggcggactgg gtgctcaggt agtggttgtc gggcagcagc acggggccgt cgccgatggg
     7441 ggtgttctgc tggtagtggt cggcgagctg cacgctgccg tcctcgatgt tgtggcggat
     7501 cttgaagttc accttgatgc cgttcttctg cttgtcggcc atgatataga cgttgtggct
     7561 gttgtagttg tactccagct tgtgccccag gatgttgccg tcctccttga agtcgatgcc
     7621 cttcagctcg atgcggttca ccagggtgtc gccctcgaac ttcacctcgg cgcgggtctt
     7681 gtagttgccg tcgtccttga agaagatggt gcgctcctgg acgtagcctt cgggcatggc
     7741 ggacttgaag aagtcgtgct gcttcatgtg gtcggggtag cggctgaagc actgcacgcc
     7801 gtaggtcagg gtggtcacga gggtgggcca gggcacgggc agcttgccgg tggtgcagat
     7861 gaacttcagg gtcagcttgc cgtaggtggc atcgccctcg ccctcgccgg acacgctgaa
     7921 cttgtggccg tttacgtcgc cgtccagctc gaccaggatg ggcaccaccc cggtgaacag
     7981 ctcttcgccc ttgctcacca tggtcactgt ctgctttgct gttggtctgg gctcctgggt
     8041 cactggctta ctaatggagt ctttatgtat gaggactctt atcaattgtt cttctataaa
     8101 ggtctgcagt gtttctgttc gtcccctaca tggacaccca gagcctccta aatacaggag
     8161 ccctgataac tgcacaagtg ctcagattcc agcagggtgg aaatgagata aagtgtgcag
     8221 atggggaggg ggacgtgaat gagagatttg agggatgaaa aggatggatg aacgcattga
     8281 aaatagcccc tttcacaaaa taataccagt aaattgtcat ataattaatg accagacctt
     8341 tacctgtaaa tgatgtgatg ttcatataca gaagaatgcc aacaatttac agatgatttt
     8401 acaaattctt aattttcctt ttctaaatgg atttgttcac acattatctc ttcaaagcaa
     8461 ttgaattttc tggaaaagat tggctgtgtg tgtaaaaggt gctactgagg aatttaatgt
     8521 gacatggaat gaagcaaaca gcagcctaag acaggatggc aggaaaaatg tcacatgttt
     8581 aaaataagag tgaaaccaac gacctggact tatagagact gtgtgtcctt aggcaaagct
     8641 cttgtacaaa cagtgcttgt gactgtgaaa ttcggggttt gcctggattg tgttaaatgt
     8701 gtgcttggat gtatcataaa aaagaatcgg ttctctgtat ttgacatttt ctgtaattta
     8761 tctaaagtta caactggcta tgcctgattt ttatcactat tgaatcaatc attttaaaga
     8821 atgttaagtc tgctgatttg gagagctcat ttacatttat tttgaatgtc tcttatttaa
     8881 cacaactgat ttaagcttta gatctgcgaa gatacggcca cgggtgctct tgatcctgtg
     8941 gctgattttg gactgtgctg ctcgcagctg ctgatgaatc acatacttcc tccattttct
     9001 tccactgatt gactgttata atttccctaa tttccaggtc aaggtgctgt gcattgtggt
     9061 aatagatgtg acatgattgc attcatttta tgtttcaggt tcagggggag gtgtgggagg
     9121 ttttttaaag caagtaaaac ctctacaaat gtggtatggc tgattatgat cctctagatc
     9181 agatccacta gttctagagc ggccattacc ctgttatccc taggccgcca ccgcggtgga
     9241 gctccaattc gccctatagt gagtcgtatt acgcgcgctc actggccgtc gttttacaac
     9301 gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca catccccctt
     9361 tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa cagttgcgca
     9421 gcctgaatgg cgaatggaaa ttgtaagcgt taatattttg ttaaaattcg cgttaaattt
     9481 ttgttaaatc agctcatttt ttaaccaata ggccgaaatc ggcaaaatcc cttataaatc
     9541 aaaagaatag accgagatag ggttgagtgt tgttccagtt tggaacaaga gtccactatt
     9601 aaagaacgtg gactccaacg tcaaagggcg aaaaaccgtc tatcagggcg atggcccact
     9661 acgtgaacca tcaccctaat caagtttttt ggggtcgagg tgccgtaaag cactaaatcg
     9721 gaaccctaaa gggagccccc gatttagagc ttgacgggga aagccggcga acgtggcgag
     9781 aaaggaaggg aagaaagcga aaggagcggg cgctagggcg ctggcaagtg tagcggtcac
     9841 gctgcgcgta accaccacac ccgccgcgct taatgcgccg ctacagggcg cgtcaggtgg
     9901 cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa
     9961 tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa
    10021 gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct
    10081 tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg
    10141 tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg
    10201 ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt
    10261 atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga
    10321 cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga
    10381 attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac
    10441 gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg
    10501 ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac
    10561 gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct
    10621 agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct
    10681 gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg
    10741 gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat
    10801 ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg
    10861 tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat
    10921 tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct
    10981 catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa
    11041 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
    11101 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
    11161 gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta
    11221 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
    11281 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
    11341 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
    11401 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc
    11461 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
    11521 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
    11581 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
    11641 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca
    11701 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg
    11761 agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc
    11821 ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag
    11881 ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag
    11941 ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg
    12001 tggaattgtg agcggataac aatttcacac aggaaacagc tatgaccatg attacgccaa
    12061 gcgcgcaatt aaccctcact aaagggaaca aaagctgggt accgggcccc ccctcgaatt
    12121 accctgttat ccctatcgag caaaatcaat cctaatgtaa aatagtatac gttcatcatt
    12181 ataatgcaaa cactgtagaa ctgttgtgtt gtgccttgca tttcattctc agtttcttat
    12241 cgtctgtctc tttttacgac agagctagaa aaaactagcc ttcatttgcc atcgccgtat
    12301 gcaaatccac tccagaactc aacaggtttg ttctgcggcg ggtgtaagtg tgaacaccgg
    12361 catgtaagtg cgcttgagca ggagaataaa gcgctcagag acaccggagg aatcctttat
    12421 tttgcgttgc gcctcagggg attcgctgag ccttctcttc atggccctgc tcttcagaca
    12481 caggtgctcc gctgtttgtg tttt
//
