LOCUS       sequence-443355-       13276 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express LYZ gene in mesenchymal lineage of Zebrafish..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13276)
  AUTHORS   Ali RH, Orellana EA, Lee SH, Chae YC, Chen Y, Clauwaert J, Kennedy 
            AL, Gutierrez AE, Papke DJ, Valenzuela M, Silverman B, Falzetta A, 
            Ficarro SB, Marto JA, Fletcher CDM, Perez-Atayde A, Alcindor T, 
            Shimamura A, Prensner JR, Gregory RI, Gutierrez A
  TITLE     A methyltransferase-independent role for METTL1 in tRNA 
            aminoacylation and oncogenic transformation.
  JOURNAL   Mol Cell. 2025 Mar 6;85(5):948-961.e11. doi: 
            10.1016/j.molcel.2025.01.003. Epub 2025 Jan 31.
  PUBMED    39892392
REFERENCE   2  (bases 1 to 13276)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2025.01.003"; journalName: "Mol Cell"; date: 
            "2025-03-6- 6"; volume: "85"; issue: "5"; pages: "948-961.e11"
COMMENT     Sequence Label: rag2:LYZ_CE_pISceI
FEATURES             Location/Qualifiers
     source          1..13276
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(1998..2018)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(1998..2016)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(2037..2053)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2037..2053)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2050..2072)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2061..2077
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2085..2091,2092..2109,2110..2115))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2130..2151
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(2268..2285)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2439..3027)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2519..2538)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3198..3989,3990..4058))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3821..3840
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4059..4163)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      4190..4645
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4277..4296)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4487..4508
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     4771..4793
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     4785..4802
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     4786..4802
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        4812..4830
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     4885..4901
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4885..4901
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     CDS             join(6082..6084,6085..6087,6088..6801)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(6082..6084,6085..6087,6088..6798)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(6127..6148)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(6388..6407)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     6735..6756
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    7103..7237
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     polyA_signal    7258..7392
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
ORIGIN
        1 aagaaacacg cgcctgagaa cgaggttgcg gtggagggcc agctcttcct cttttcaagg
       61 cgaagacagc atgckkkrkg ywwcaggrtc tccwgagctc ggrrayryag gtagacctgm
      121 tggtttatac tggggcatta ggacccacac agaccacagg ttgatctgct ggtttatacc
      181 ggggcattag gacccacaca gaccacaggt tgacatgctg gtttgtaccg gggcattagg
      241 acccacacag accacaggtt gacctgctgg tttatactgg ggcattagga cccacacaga
      301 ccacaggttg acctgctggt ttgtactggg gcatttggac ccacacagac cacaggttga
      361 cctgctggtt tatactgggg cattagracc cacacagacc acaggttgac ctgctggttt
      421 atactggkgc attaggaccc acacagacca caggttgacc tgctggttta tactggggca
      481 ttaggaccca cacagaccac aggttgacct gctggtttat actggggcat taggacccac
      541 acagaccaca ggttgacctg ctggtttata ctggggcatt aggacccaca cagaccacag
      601 gtwgacctgc tggtttatac tggggcatta ggacccacac agacyacagg ttgacctgct
      661 ggtttatact ggggcattag gacccacaca gaccacaggt agacctgctg gtttatactg
      721 gggcattagg acccacacag accacaggtt gacctgctgg tttatactgg ggcattagga
      781 cccacacaga ccacaggtag acctgctggt ttactaacac ctcttccggg agcaacctag
      841 ctttcccatg tggtctccct tttaggtact tgccggacac ttcagctgct ggtcaagagg
      901 ctagctaaag ctaaaatcat aaatatacat ttaaaacaga ttagttttat ataacatatt
      961 aattaaaact aaattgaact ttaaacatct tttaattgcc tgtgattgta taatatttca
     1021 cagattgttc ccattcgttg gtcgtgtcta gttagcgcac atgcaatcgc aacagatttt
     1081 cctttaagac ctgcattcga cactgaaatc acactcttgg taatgcatct gtgacagtct
     1141 ggatgtaatg tgctgttaaa atagtctcct cggtgtgcat ctgagtggtc tgtggcatcg
     1201 ggttcggtgt ggagcagaat gattcactct cattacacca aatatcgaat gcgtgagtca
     1261 cacatcacat tttggcagga ggggaagctc ggattttgta aaaattctcc ttatttcccg
     1321 caggcttata tttgcattaa ctgtttctgc ttggcggctc tttaaaggaa cgagagaaat
     1381 taggcgactg ttgttgaaag aacgagagag agagtgaaga agggaacagc tgtgccttcc
     1441 tcattagcat aatgtttttg tttttcggtg ggggcttcga gccctcgtgc agttgtgtgt
     1501 ttgttttgga ggccgagaga tgtgtgtgca agagtgcaaa acaaaaaccc gtaaccacga
     1561 gtgcctgaag aacagtgaaa aacacaaaca gcggagcacc tgtgtctgaa gagcagggcc
     1621 atgaagagaa ggctcagcga atcccctgag gcgcaacgca aaataaagga ttcctccggt
     1681 gtctctgagc gctttattct cctgctcaag cgcacttaca tgccggtgtt cacacttaca
     1741 cccgccgcag aacaaacctg ttgagttctg gagtggattt gcatacggcg atggcaaatg
     1801 aaggctagtt ttttctagct ctgtcgtaaa aagagacaga cgataagaaa ctgagaatga
     1861 aatgcaaggc acaacacaac agttctacag tgtttgcatt ataatgatga acgtatacta
     1921 ttttacatta ggattgattt tgctcgatag ggataacagg gtaattcgag ggggggcccg
     1981 gtacccagct tttgttccct ttagtgaggg ttaattgcgc gcttggcgta atcatggtca
     2041 tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga
     2101 agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg
     2161 cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc
     2221 caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac
     2281 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata
     2341 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa
     2401 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct
     2461 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
     2521 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
     2581 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca
     2641 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
     2701 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
     2761 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
     2821 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga
     2881 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc
     2941 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag
     3001 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac
     3061 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc
     3121 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag
     3181 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt
     3241 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag
     3301 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca
     3361 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact
     3421 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca
     3481 gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg
     3541 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc
     3601 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg
     3661 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca
     3721 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt
     3781 atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc
     3841 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc
     3901 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca
     3961 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa
     4021 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat
     4081 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa
     4141 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga cgcgccctgt
     4201 agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc
     4261 agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc
     4321 tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg
     4381 cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga
     4441 tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc
     4501 caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg
     4561 ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt
     4621 aacaaaatat taacgcttac aatttccatt cgccattcag gctgcgcaac tgttgggaag
     4681 ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa
     4741 ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca
     4801 gtgagcgcgc gtaatacgac tcactatagg gcgaattgga gctccaccgc ggtggcggcc
     4861 tagggataac agggtaatgg ccgctctaga actagtggat ctgatctaga ggatcataat
     4921 cagccatacc acatttgtag aggttttact tgctttaaaa aacctcccac acctccccct
     4981 gaacctgaaa cataaaatga atgcaatcat gtcacatcta ttaccacaat gcacagcacc
     5041 ttgacctgga aattagggaa attataacag tcaatcagtg gaagaaaatg gaggaagtat
     5101 gtgattcatc agcagctgcg agcagcacag tccaaaatca gccacaggat caagagcacc
     5161 cgtggccgta tcttcgcaga tctaaagctt aaatcagttg tgttaaataa gagacattca
     5221 aaataaatgt aaatgagctc tccaaatcag cagacttaac attctttaaa atgattgatt
     5281 caatagtgat aaaaatcagg catagccagt tgtaacttta gataaattac agaaaatgtc
     5341 aaatacagag aaccgattct tttttatgat acatccaagc acacatttaa cacaatccag
     5401 gcaaaccccg aatttcacag tcacaagcac tgtttgtaca agagctttgc ctaaggacac
     5461 acagtctcta taagtccagg tcgttggttt cactcttatt ttaaacatgt gacatttttc
     5521 ctgccatcct gtcttaggct gctgtttgct tcattccatg tcacattaaa ttcctcagta
     5581 gcacctttta cacacacagc caatcttttc cagaaaattc aattgctttg aagagataat
     5641 gtgtgaacaa atccatttag aaaaggaaaa ttaagaattt gtaaaatcat ctgtaaattg
     5701 ttggcattct tctgtatatg aacatcacat catttacagg taaaggtctg gtcattaatt
     5761 atatgacaat ttactggtat tattttgtga aaggggctat tttcaatgcg ttcatccatc
     5821 cttttcatcc ctcaaatctc tcattcacgt ccccctcccc atctgcacac tttatctcat
     5881 ttccaccctg ctggaatctg agcacttgtg cagttatcag ggctcctgta tttaggaggc
     5941 tctgggtgtc catgtagggg acgaacagaa acactgcaga cctttataga agaacaattg
     6001 ataagagtcc tcatacataa agactccatt agtaagccag tgacccagga gcccagacca
     6061 acagcaaagc agacagtgac catggtgagc aagggcgaag agctgttcac cggggtggtg
     6121 cccatcctgg tcgagctgga cggcgacgta aacggccaca agttcagcgt gtccggcgag
     6181 ggcgagggcg atgccaccta cggcaagctg accctgaagt tcatctgcac caccggcaag
     6241 ctgcccgtgc cctggcccac cctcgtgacc accctgacct acggcgtgca gtgcttcagc
     6301 cgctaccccg accacatgaa gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac
     6361 gtccaggagc gcaccatctt cttcaaggac gacggcaact acaagacccg cgccgaggtg
     6421 aagttcgagg gcgacaccct ggtgaaccgc atcgagctga agggcatcga cttcaaggag
     6481 gacggcaaca tcctggggca caagctggag tacaactaca acagccacaa cgtctatatc
     6541 atggccgaca agcagaagaa cggcatcaag gtgaacttca agatccgcca caacatcgag
     6601 gacggcagcg tgcagctcgc cgaccactac cagcagaaca cccccatcgg cgacggcccc
     6661 gtgctgctgc ccgacaacca ctacctgagc acccagtccg ccctgagcaa agaccccaac
     6721 gagaagcgcg atcacatggt cctgctggag ttcgtgaccg ccgccgggat cactctcggc
     6781 atggacgagc tgtacaagta aagcggcccc tctcgagcct ctagaactat agtgagtcgt
     6841 attacgtaga tccagacatg ataagataca ttgatgagtt tggacaaacc acaactagaa
     6901 tgcagtgaaa aaaatgcttt atttgtgaaa tttgtgatgc tattgcttta tttgtaacca
     6961 ttataagctg caataaacaa gagatctgat ctagaggatc ataatcagcc ataccacatt
     7021 tgtagaggtt ttacttgctt taaaaaacct cccacacctc cccctgaacc tgaaacataa
     7081 aatgaatgca attgttgttg ttaacttgtt tattgcagct tataatggtt acaaataaag
     7141 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt
     7201 gtccaaactc atcaatgtat cttatcatgt ctggatcatc atcgaattgt tgttgttaac
     7261 ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat
     7321 aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat
     7381 catgtctgga tcatcatcga tttacactcc acaaccttga acatactgac ggacatctct
     7441 gttttgacaa cgatttctcc atgccaccca tgctctaatg ccttgtggat cacggacaac
     7501 cctctttgca caagctacag catcagcgat gttatcttgc agcaaagcac tgcaggataa
     7561 atgacaggca ttaactgctc ctggggtttt gccatcatta caccagtagc ggctattgat
     7621 ctgaaatatc ccataatcag tgcttctgtc tccagcattg tagtttgtag ctcgtgtgtt
     7681 gtaaccactc tcccatttgg ccaaacacat ccagtttgct aggctgattc ccctgtagcc
     7741 atccattccc aatcttttca gagttctggc caactcacac ctttcaaaga ccttgccctg
     7801 gaccgtaaca gaaaggagga caagccccag aacaatgaga gccttcatgg atcccttttt
     7861 gaaggtagct gtgtaaaaaa aataaaaaaa aacattaatt tatagtcatt taattgtaca
     7921 atgttaattt tattacctct tataaaaacc aaccatggct ttaccacaga taatacttaa
     7981 acttaaagat aacaatggca accatagtgt tacactacac tagacatagt ttaaccatag
     8041 tatttgtagt ataactgtga tgtttaaatg attatcattg tatcaaagaa tgctagtgct
     8101 tcaaattagc ataagagtaa gcgaagtgtg tagttaggtc ttattttatt attcaaagta
     8161 agaaaaaaac gcggaaccat atctttcttt cgctgtttcc ccgaaaacag aaacattttc
     8221 cacaggaaat tgtagttttc ctaactctag tttcccgttg agacgcacat taagcataca
     8281 atataaggac tacagtaccc agcattcagt gcgagagcgg cgtctgcgtt taaacggtag
     8341 gctttatctg atgtactttt ctatgtgctc caaaagctac tattctcgta ttttcttcaa
     8401 catttttctg ccgtttctac atctgatatg ttttagattc gcctgaaggt aatcaaatac
     8461 gcaagagaag cattaacaaa caaattagca agcactaata ataactgtca acacgagtaa
     8521 ctgagcacta cattaacaca gtaacgttat atgtaaacca cgttacctac tgtagcctat
     8581 attttaatat atatcatttt acattatgca aatcgtattt ccatcagttc ctctactcta
     8641 tatgtcactt aaaacacgta cgcttccaaa tattacatat ctagtcttac aatagtcgtc
     8701 ctagtaatgt aacgttactt cttacctctt ggtcagtgta ctccgaaatc tgagagctgt
     8761 ttggtgctgg aaatttttat tttagatatt aacgcgaatt gtttagtgag tgagccagag
     8821 gttgagtgtt tgtgaaggtt gactggctcc tcttgaggtt tactgtttgc aactgctgct
     8881 cacaacaggt gtttgagcac aatcacacga gtttgaatga aaacatgcac aaactcactg
     8941 catcattggc tgtttattaa tatggagttc tggttttgtg ctgctgatca gcaatactca
     9001 tagatattat atttttccgt gcaattttag tcattatgaa tgtttccagg tctttccaaa
     9061 tggggacata acccaaccac cccccgcgct tgatacggtt ctgtatatgc atatattcac
     9121 agtaatactt ttgtatagta accaatagca taggttaata tacaaaaaaa aaaaaaaaat
     9181 ccaaattgat aataatatac atttttagca atgtgttaat attttctgaa ggatcatgtg
     9241 acactaaaat tcagattgaa tacaatttag ctttggcatt aaagaaataa cattgtcaaa
     9301 aatatttaaa atataatata gttattttaa atggaaacaa tgtatcccga tattgctttt
     9361 tcgactgtaa ttgtgatcaa ataaatgcag acttggtgac ataaagtttt ttttttaaat
     9421 agtgctgtaa attatttgga ttatctagat atccttaaca atatacactc accggccact
     9481 ttattatgta caccccacta gtactgggtt ggacctcttt ttgccttagc atagattccc
     9541 ttccttttgc atttcaacca ggtactggaa aattcctcag agattttggt ccatattgac
     9601 atgctagcat catgcagatt tgtcggctgc acatccagga tgcgaatctt ccattctacc
     9661 acaccccaaa ggcgctctat tggattgaga tctggtgact gtggaggtca ttcgagcaca
     9721 gtgaactcat tgtcatgttc aagaaaccag tctgagatca ttcacgcttt atgacatggt
     9781 gcattatcct gcaggaagta gccatcagaa gatgggtaca ctgtggtcaa aaagaaatga
     9841 acatggtcag caacaatact cagttaggct gtggcgttga cacgatggtt aattggtact
     9901 aatgggccca aagtgtgcca agaatatatc ccccacacca ttacaccacc atcaccagcc
     9961 tgaactgttg atacaaggta gaatgtatcc atctgaccag gcaacataat gttcaatctt
    10021 ctattgtcta cttatgttga gcctgtgcga attgtagcct cagtttcctg ttcttagctg
    10081 acaggagtgg cacccgatgt ggtcttgagc tgctgtagcc cacccgcctc aaggttcgac
    10141 atgttgtgtt caaaggtgct cttctgcatg cctcagttct aacgagtggt tatttgaatt
    10201 actgttgcct ttctatcagc tggaaccagt caggccattc tcctctgacc tctggcatca
    10261 acaagacatt tgcccccaca gaactgccgc tcactggata ttatcttttt ttggaccatt
    10321 ctctgtaaac cccagagatg tttgtgtgtg aaaaccccag taaatcagca gtttctgaaa
    10381 tactcagacc agcccgtctg gcaccaacaa acacgtcacg tttaaagtca cttaaatcac
    10441 cttgcttctc cattctgatg ctcgctttga actgcagcag atcgtcttga ccatgtctac
    10501 atgcctaaat gcactgagtt gctgcctggt gattggctga ttagaaattt acgttaacaa
    10561 gcagttagac aggtgtacct aataaagtgg ccggtgagta tttttcagat tttttttttt
    10621 caaattttaa atagtgaaat catattatta tcagacaatc accatcaaag tacaacactg
    10681 tttacaggca attaaattaa actaattctg ctttgaagtc gtatttacat gcaatttgag
    10741 actgaatatt caaagtagcg taataaacaa tataaagagc atcacaagtt gtcactgatt
    10801 aaaaataacc aaatgtgtga ataataaaac aattctacct caatctttaa aattcatata
    10861 ttttattaat catgcattta tttacatttt acgtaaacat tttcatacat ttattttctt
    10921 ttcggcttag tctctttatt aatctggggt taaacgcacg cgaatgctcc acacagaaac
    10981 atcaagtgat ccagctgagg ctcgaaccag cgaccttctt gctttgctgg cgacagcact
    11041 acctactgca ccaccgtgtc gttgtaaaca ttattttgta taatttattt aaaacaattt
    11101 aattatttta aattacgttt gtttttagtt ttttatacta atgctgctta tcttgtttat
    11161 caattttact ataattttca acaatgaaat tattaattat tttagtttta tttttactta
    11221 taaaattttt ttttttcccc caaacatttc attagcccta tagcacttcc ttgcagctcc
    11281 ctgaggtacc ccagggcaaa gttttaatgg cccctagctc tggcacaagt tatccagaaa
    11341 gttccattat aataagctaa tgataattcc ttacatttat atcgcgcttt tctggaaact
    11401 caaaacactt ttttgggcaa aatctcctca tcaaccacca gtgtgcagca tccacctgga
    11461 tgacgcgatg gcagccatat tgcaccagac cgcacactac acaccagtat attggtggag
    11521 aggagacagg ggctgcgtcc gaaaccgtct actactactt agtaggtact gtatttgaat
    11581 ttaaacttac tacttggctg tgggaaaaga acattctata aaatatgaat gtgagcagta
    11641 tgaatggaac ttggacgtac tacatccgcc aatatgtcat cgtcacgtaa cctacccgca
    11701 tgagttgcgt tgcttctctc cgattcaaga attctctcac ggggcatcat ggaatagcgt
    11761 agcgtgcatc gaatgcacac tctagaatct cgccggaagt attcggacat attacttggc
    11821 tcgcatactg attttagcgt acctcataaa atggaagtat gcgatttcgg ccccagctag
    11881 agtgataaag ccaattatga tatggggatg gttaggaggc catgatggac agaggacagt
    11941 gagcaaattt gtccaggatg ccagggtagg aaggacatcc tgggaatttt ttaccaccac
    12001 agagagtcgg gaccttgttt taacatctta tccgaaagat ggagctcacc gagcagtata
    12061 gagtctccat caatatactg gggcattagg acccacacag accacaggtt gacctgctgg
    12121 tttatactgg ggcattagga cccacacaga ccacaggttg acctgctggt ttatcctggg
    12181 gcattaggac ccacacagac cacaggttga tctgctggtt tataccgggg cattaggacc
    12241 cacacagacc acaggttgac atgctggttt atcctgggac tttaggaccc acacagacca
    12301 caggttgatc tgctggttta tactggggca ttaggaccca cacagaccac aggttgatct
    12361 gctggtttat accggggcat taggacccac acagaccaca ggttgacatg ctggtttgta
    12421 ccggggcatt aggacccaca cagaccacag gttkacctgc tggttwatmc tkskgcatta
    12481 gracccrccm adaccmmagg tbvamyhgck wrayyttayt sskmcattyb rmnscaatac
    12541 acagaccaca ggttgatctg ctggtttata ccggggcatt aggacccaca cagaccacag
    12601 gttgacatgc tggtttatcc gcattaggac ccacacagac cacaggttga catgctggtt
    12661 tatcctggga ctttaggacc cacacagacc acaggttgat ctgctggttt atacctgggc
    12721 atcagtacca acacagacta caggttaatc tgctggttta gacctgtgga ttagccacat
    12781 cacacgccta aggtaaacct gttggtttat accatggcac tatccacacc atagtcaaac
    12841 aattaatctg gtgatttgta taggcgttta atgcattcaa catgcacata ctcatatact
    12901 cagtgtaatc tgatcactat ctccacactg tgagtatcat agctccacct atcattcaaa
    12961 ttcgtgtcac tatactgcag cagaaggagg tatcctaggt gctgcgatgt gtcgtaggct
    13021 ttgattttca tataagcggc aggtcccaga tagaccaaag gttcatctgc ttggttgtgc
    13081 ctgggcgtga tgacccacac cgaccacagg ttgatttgct ggtttatatc ggggcattag
    13141 gacccacaca gcccacaggt tgacctgctg gtttatacca aggcaatagg acccacacag
    13201 accacacgtt tacctgctgg tttatactgg ggcattagga cccacacaga ccacaggttg
    13261 acctgctggt ttatac
//
