LOCUS       sequence-443416-        3854 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  For competition during selection with hybrid circuit. Expresses MBP 
            fused to evolved degron SD55.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3854)
  AUTHORS   Mercer JAM, DeCarlo SJ, Roy Burman SS, Sreekanth V, Nelson AT, 
            Hunkeler M, Chen PJ, Donovan KA, Kokkonda P, Tiwari PK, Shoba VM, 
            Deb A, Choudhary A, Fischer ES, Liu DR
  TITLE     Continuous evolution of compact protein degradation tags regulated 
            by selective molecular glues.
  JOURNAL   Science. 2024 Mar 15;383(6688):eadk4422. doi: 
            10.1126/science.adk4422. Epub 2024 Mar 15.
  PUBMED    38484051
REFERENCE   2  (bases 1 to 3854)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2024-03-15"; volume: "383(6688):eadk4422. doi"; pages: " 
            10.1126/science.adk4422. Epub 2024 Mar 15"
COMMENT     Sequence Label: pSD103D
FEATURES             Location/Qualifiers
     source          1..3854
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(1..792)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     promoter        complement(793..884)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      893..1481
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1382..1401
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1635..1652
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    1667..1807
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(1733..1752)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     1893..1915
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(1909..2100)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     CDS             3562..3591
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
ORIGIN
        1 ttatttgccg actaccttgg tgatctcgcc tttcacgtag tggacaaatt cttccaactg
       61 atctgcgcgc gaggccaagc gatcttcttc ttgtccaaga taagcctgtc tagcttcaag
      121 tatgacgggc tgatactggg ccggcaggcg ctccattgcc cagtcggcag cgacatcctt
      181 cggcgcgatt ttgccggtta ctgcgctgta ccaaatgcgg gacaacgtaa gcactacatt
      241 tcgctcatcg ccagcccagt cgggcggcga gttccatagc gttaaggttt catttagcgc
      301 ctcaaataga tcctgttcag gaaccggatc aaagagttcc tccgccgctg gacctaccaa
      361 ggcaacgcta tgttctcttg cttttgtcag caagatagcc agatcaatgt cgatcgtggc
      421 tggctcgaag atacctgcaa gaatgtcatt gcgctgccat tctccaaatt gcagttcgcg
      481 cttagctgga taacgccacg gaatgatgtc gtcgtgcaca acaatggtga cttctacagc
      541 gcggagaatc tcgctctctc caggggaagc cgaagtttcc aaaaggtcgt tgatcaaagc
      601 tcgccgcgtt gtttcatcaa gccttacggt caccgtaacc agcaaatcaa tatcactgtg
      661 tggcttcagg ccgccatcca ctgcggagcc gtacaaatgt acggccagca acgtcggttc
      721 gagatggcgc tcgatgacgc caactacctc tgatagttga gtcgatactt cggcgatcac
      781 cgcttccctc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct
      841 catgagcgga tacatatttg aatgtattta gaaaaatagg ccaaataggc cgttgagatc
      901 ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg
      961 tttgtttgcc ggatcaagag ctaccacctc tttttccgaa ggtaactggc ttcagcagag
     1021 cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact
     1081 ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg
     1141 gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc
     1201 ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg
     1261 aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg
     1321 cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag
     1381 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc
     1441 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct
     1501 ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc
     1561 ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc
     1621 gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt
     1681 ttctccttac gcatctgtgc ggtatttcac accgcatatg gtgcactctc agtacaatct
     1741 gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat
     1801 ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc
     1861 ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc
     1921 accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag
     1981 cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt
     2041 taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac
     2101 tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga
     2161 gaggatgctc acgatacggg ttactgatga tgaacaagag gacatccggt caaataaaac
     2221 gaaaggctca gtcgaaagac tgggcctttc gttttgctga ggattctaga gcacagctaa
     2281 caccacgtcg tccctatctg ctgccctagg tctatgagtg gttgctggat aactttacgg
     2341 gcatgcataa ggctcgtata atatattcag ggagaccaca acggtttccc tctacaaata
     2401 attttgttta acttttacta gagtaaggag gaaaaaaaaa tgaagatcga agaaggcaag
     2461 ttagtgattt ggatcaacgg cgataaaggc tataacggtt tagcggaagt aggcaagaag
     2521 tttgagaagg ataccggaat caaagtgacg gtcgaacacc cggataagtt agaggagaag
     2581 ttccctcaag tagcggcaac gggcgacggt cctgacatca tcttttgggc acacgaccgc
     2641 ttcggtggtt acgctcagtc aggtttatta gcggagatta ctccagacaa agcgttccag
     2701 gacaagttat atccatttac gtgggacgca gtgcgttata acggcaagtt aatcgcatat
     2761 cccattgcgg tagaagccct gagcctgatt tacaacaagg acctgttacc caaccctccg
     2821 aagacgtggg aggagattcc ggctttagat aaggaactga aagcgaaagg caagtctgcc
     2881 ctgatgttta acctgcaaga accctacttc acctggcctc tgatcgcggc ggatggcggt
     2941 tatgcgttta agtacgaaaa cggcaaatac gatattaagg atgtaggcgt cgataacgcc
     3001 ggtgccaaag ccggtctgac ctttttagtt gatctgatta agaataagca catgaacgca
     3061 gacaccgact acagcattgc ggaagctgcg tttaataaag gtgagaccgc catgacgatt
     3121 aacggacctt gggcgtggtc gaacattgat accagtaaag tcaattacgg cgttacagtc
     3181 ctgccgacct tcaaaggtca gccgtcaaaa ccgttcgtag gtgtcttatc cgccggtatc
     3241 aacgcggcgt ccccaaataa agagttggct aaagagttcc tggaaaacta tctgctgaca
     3301 gacgaaggac tggaggctgt gaacaaggac aagccactgg gcgctgttgc gctgaaaagt
     3361 tatgaggaag aactggcgaa agatccccgc atcgcggcga cgatggaaaa cgcccaaaaa
     3421 ggcgaaatca tgccgaacat tccgcagatg tcagcttttt ggtatgccgt acgcacggct
     3481 gttattaacg ccgcgtcggg ccgccaaacc gttgatgagg cactgaagga cgcgcagact
     3541 cgtatcacca aggcggccgc ggaacaaaag cttatttctg aagaggactt gttcaatgta
     3601 ctgatggtcc ataaacggag tcacactggc gagctcctgc tcttttgtcc tatttgcggg
     3661 ttcacgtgtc ggcagaaggg caacctcctc cggcatatca atctgcaccc gggtgaaaaa
     3721 ctgtttaggt accatctcta caattacgcc tgtcagagaa gagatgcttt gtaacctaga
     3781 aggagatttt caacatggga aatataatga ccctcttgat aacccaagag ggcatttttt
     3841 aatgcccatg gcgt
//
