LOCUS       sequence-443425-        4971 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  AAV-STARR-seq screening vector with 4.26 promote.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4971)
  AUTHORS   Chan YC, Kienle E, Oti M, Di Liddo A, Mendez-Lago M, Aschauer DF, 
            Peter M, Pagani M, Arnold C, Vonderheit A, Schon C, Kreuz S, Stark 
            A, Rumpel S
  TITLE     An unbiased AAV-STARR-seq screen revealing the enhancer activity map
            of genomic regions in the mouse brain in vivo.
  JOURNAL   Sci Rep. 2023 Apr 25;13(1):6745. doi: 10.1038/s41598-023-33448-w.
  PUBMED    37185990
REFERENCE   2  (bases 1 to 4971)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2023-04-25"; volume: "13(1):6745. doi"; pages: " 
            10.1038/s41598-023-33448-w"
COMMENT     Sequence Label: pAAV_SC_4.26_STARR-seq
FEATURES             Location/Qualifiers
     source          1..4971
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..130
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
     promoter        194..224
                     /label=minP
                     /note="minimal TATA-box promoter with low basal activity"
     intron          251..383
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     366..385
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     promoter        join(518..523,524..541,542..548)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             602..1261
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(668..687)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             1603..1908
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     1787..1806
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     polyA_signal    1986..2107
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1997..2016)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     2051..2070
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      2449..2904
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2536..2555)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2746..2767
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(2921..2940)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     3040..3062
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(3100..3118)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        3186..3290
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3291..3359,3360..4151)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3509..3528)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4322..4910
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4811..4830
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcga ttcacacggt acctcagcca gtaagatctg ctaacctcga
      181 gtgagaggta ccgagagggt atataatgga agctcgactt ccaggggccc ggtcgtgagg
      241 cactgggcag gtaagtatca aggttacaag acaggtttaa ggagaccaat agaaactggg
      301 cttgtcgaga cagagaagac tcttgcgttt ctgataggca cctattggtc ttactgacat
      361 ccactttgcc tttctctcca caggtgtcca ccctgcaggc ttaagcatgg ctagcaaagg
      421 agaagaactc ttcactggag ttgtcccaat tcttgttgaa ttagattgat ctagagcatg
      481 caccggtgat atcgcggccg cattaggcac cccaggcttt acactttatg cttccggctc
      541 gtataatgtg tggattttga gttaggatcc gtcgagattt tcaggagcta aggaagctaa
      601 aatggagaaa aaaatcactg gatataccac cgttgatata tcccaatggc atcgtaaaga
      661 acattttgag gcatttcagt cagttgctca atgtacctat aaccagaccg ttcagctgga
      721 tattacggcc tttttaaaga ccgtaaagaa aaataagcac aagttttatc cggcctttat
      781 tcacattctt gcccgcctga tgaatgctca tccggaattc cgtatggcaa tgaaagacgg
      841 tgagctggtg atatgggata gtgttcaccc ttgttacacc gttttccatg agcaaactga
      901 aacgttttca tcgctctgga gtgaatacca cgacgatttc cggcagtttc tacacatata
      961 ttcgcaagat gtggcgtgtt acggtgaaaa cctggcctat ttccctaaag ggtttattga
     1021 gaatatgttt ttcgtctcag ccaatccctg ggtgagtttc accagttttg atttaaacgt
     1081 ggccaatatg gacaacttct tcgcccccgt tttcaccatg ggcaaatatt atacgcaagg
     1141 cgacaaggtg ctgatgccgc tggcgattca ggttcatcat gccgtttgtg atggcttcca
     1201 tgtcggcaga atgcttaatg aattacaaca gtactgcgat gagtggcagg gcggggcgta
     1261 aacgcgtgga tccggcttac taaaagccag ataacagtat gcgtatttgc gcgctgattt
     1321 ttgcggtata agaatatata ctgatatgta tacccgaagt atgtcaaaaa gaggtatgct
     1381 atgaagcagc gtattacagt gacagttgac agcgacagct atcagttgct caaggcatat
     1441 atgatgtcaa tatctccggt ctggtaagca caaccatgca gaatgaagcc cgtcgtctgc
     1501 gtgccgaacg ctggaaagcg gaaaatcagg aagggatggc tgaggtcgcc cggtttattg
     1561 aaatgaacgg ctcttttgct gacgagaaca ggggctggtg aaatgcagtt taaggtttac
     1621 acctataaaa gagagagccg ttatcgtctg tttgtggatg tacagagtga tattattgac
     1681 acgcccgggc gacggatggt gatccccctg gccagtgcac gtctgctgtc agataaagtc
     1741 tcccgtgaac tttacccggt ggtgcatatc ggggatgaaa gctggcgcat gatgaccacc
     1801 gatatggcca gtgtgccggt ctccgttatc ggggaagaag tggctgatct cagccaccgc
     1861 gaaaatgaca tcaaaaacgc cattaacctg atgttctggg gaatataaat gtcaggctcc
     1921 cttatacaca gccagtctgc aggatatcgt cgacgaattc ggccggccgc ttcgagcaga
     1981 catgataaga tacattgatg agtttggaca aaccacaact agaatgcagt gaaaaaaatg
     2041 ctttatttgt gaaatttgtg atgctattgc tttatttgta accattataa gctgcaataa
     2101 acaagttaac aacaacaatt gcattcattt tatgtttcag gttcaggggg aggtgtggga
     2161 ggttttttaa agcaagtaaa acctctacaa atgtggtaaa atcgataagg atccgaccga
     2221 tgcccttgag agccttcagc gctttgacgg ttaccaccca ctccctctct gcgcgctcgc
     2281 tcgctcactg aggccgggcg accaaaggtc gcccgacgcc cgggctttgc ccgggcggcc
     2341 tcagtgagcg agcgagcgcg cagctgcctg caggggcgcc tgatgcggta ttttctcctt
     2401 acgcatctgt gcggtatttc acaccgcata cgtcaaagca accatagtac gcgccctgta
     2461 gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca
     2521 gcgccttagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct
     2581 ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc
     2641 acctcgaccc caaaaaactt gatttgggtg atggttcacg tagtgggcca tcgccctgat
     2701 agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc
     2761 aaactggaac aacactcaac tctatctcgg gctattcttt tgatttataa gggattttgc
     2821 cgatttcggt ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaatttta
     2881 acaaaatatt aacgtttaca attttatggt gcactctcag tacaatctgc tctgatgccg
     2941 catagttaag ccagccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc
     3001 tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga
     3061 ggttttcacc gtcatcaccg aaacgcgcga gacgaaaggg cctcgtgata cgcctatttt
     3121 tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa
     3181 atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca
     3241 tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc
     3301 aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc
     3361 acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt
     3421 acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt
     3481 ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg
     3541 ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact
     3601 caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg
     3661 ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga
     3721 aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg
     3781 aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa
     3841 tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac
     3901 aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc
     3961 cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca
     4021 ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga
     4081 gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta
     4141 agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc
     4201 atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc
     4261 cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt
     4321 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac
     4381 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct
     4441 tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact
     4501 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg
     4561 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata
     4621 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga
     4681 cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag
     4741 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg
     4801 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac
     4861 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca
     4921 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg t
//
