LOCUS       sequence-443433-        9257 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Stable expression of GFP-PI4KB in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9257)
  AUTHORS   Shinoda S, Sakai Y, Matsui T, Uematsu M, Koyama-Honda I, Sakamaki 
            JI, Yamamoto H, Mizushima N
  TITLE     Syntaxin 17 recruitment to mature autophagosomes is temporally 
            regulated by PI4P accumulation.
  JOURNAL   Elife. 2024 Jun 4;12:RP92189. doi: 10.7554/eLife.92189.
  PUBMED    38831696
REFERENCE   2  (bases 1 to 9257)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2024-06-4"; volume: "12:RP92189. doi"; pages: " 
            10.7554/eLife.92189"
COMMENT     Sequence Label: pMRXIP-GFP-PI4KB
FEATURES             Location/Qualifiers
     source          1..9257
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     misc_feature    739..1096
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             1161..1577
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1507..1529
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1545..1561
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     misc_feature    1587..1961
                     /label=pol region
                     /note="Moloney murine leukemia virus (MMLV) pol region 
                     containing the splice acceptor site"
     primer_bind     1899..1915
                     /label=pBMN 5'
                     /note="MMLV sequence, for inserts in pBMN retroviral 
                     vector"
     regulatory      1988..1997
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(1994..1996,1997..1999,2000..2710)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(1994..1996,1997..1999,2000..2710)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2039..2060)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2300..2319)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2647..2668
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    5168..5734
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    5184..5736
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5328..5345)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5555..5574
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             5754..6353
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5754..5773)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6250..6270
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             6583..7052
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     primer_bind     complement(7182..7199)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7353..7941)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7433..7452)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8105..8896,8897..8965))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8728..8747
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8966..9070)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     9138..9156
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg ctagagaacc atcagatgtt tccagggtgc cccaaggacc
      421 tgaaatgacc ctgtgcctta tttgaactaa ccaatcagtt cgcttctcgc ttctgttcgc
      481 gcgcttctgc tccccgagct caataaaaga gcccacaacc cctcactcgg cgcgccagtc
      541 ctccgattga ctgagtcgcc cgggtacccg tgtatccaat aaaccctctt gcagttgcat
      601 ccgacttgtg gtctcgctgt tccttgggag ggtctcctct gagtgattga ctacccgtca
      661 gcgggggtct ttcatttggg ggctcgtccg ggatcgggag acccctgccc agggaccacc
      721 gacccaccac cgggaggtaa gctggccagc aacttatctg tgtctgtccg attgtctagt
      781 gtctatgact gattttatgc gcctgcgtcg gtactagtta gctaactagc tctgtatctg
      841 gcggacccgt ggtggaactg acgagttcgg aacacccggc cgcaaccctg ggagacgtcc
      901 cagggacttc gggggccgtt tttgtggccc gacctgagtc caaaaatccc gatcgttttg
      961 gactctttgg tgcacccccc ttagaggagg gatatgtggt tctggtagga gacgagaacc
     1021 taaaacagtt cccgcctccg tctgaatttt tgctttcggt ttgggaccga agccgcgccg
     1081 cgcgtcttgt ctgctgcagc atcgttctgt gttgtctctg tctgactgtg tttctgtatt
     1141 tgtctgaaaa tatgggcccg ggccagactg ttaccactcc cttaagtttg accttaggtc
     1201 actggaaaga tgtcgagcgg atcgctcaca accagtcggt agatgtcaag aagagacgtt
     1261 gggttacctt ctgctctgca gaatggccaa cctttaacgt cggatggccg cgagacggca
     1321 cctttaaccg agacctcatc acccaggtta agatcaaggt cttttcacct ggcccgcatg
     1381 gacacccaga ccaggtcccc tacatcgtga cctgggaagc cttggctttt gacccccctc
     1441 cctgggtcaa gccctttgta caccctaagc ctccgcctcc tcttcctcca tccgccccgt
     1501 ctctccccct tgaacctcct cgttcgaccc cgcctcgatc ctccctttat ccagccctca
     1561 ctccttctct aggcgccccc atatggccat atgagatctt atatggggca cccccgcccc
     1621 ttgtaaactt ccctgaccct gacatgacaa gagttactaa cagcccctct ctccaagctc
     1681 acttacaggc tctctactta gtccagcacg aagtctggag acctctggcg gcagcctacc
     1741 aagaacaact ggaccgaccg gtggtacctc acccttaccg agtcggcgac acagtgtggg
     1801 tccgccgaca ccagactaag aacctagaac ctcgctggaa aggaccttac acagtcctgc
     1861 tgaccacccc caccgccctc aaagtagacg gcatcgcagc ttggatacac gccgcccacg
     1921 tgaaggctgc cgaccccggg ggtggaccat cctctagact gccggatctg ccatcgatat
     1981 aaagcttgcc accatggtga gcaagggcga ggagctgttc accggggtgg tgcccatcct
     2041 ggtcgagctg gacggcgacg taaacggcca caagttcagc gtgtccggcg agggcgaggg
     2101 cgatgccacc tacggcaagc tgaccctgaa gttcatctgc accaccggca agctgcccgt
     2161 gccctggccc accctcgtga ccaccctgac ctacggcgtg cagtgcttca gccgctaccc
     2221 cgaccacatg aagcagcacg acttcttcaa gtccgccatg cccgaaggct acgtccagga
     2281 gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg tgaagttcga
     2341 gggcgacacc ctggtgaacc gcatcgagct gaagggcatc gacttcaagg aggacggcaa
     2401 catcctgggg cacaagctgg agtacaacta caacagccac aacgtctata tcatggccga
     2461 caagcagaag aacggcatca aggtgaactt caagatccgc cacaacatcg aggacggcag
     2521 cgtgcagctc gccgaccact accagcagaa cacccccatc ggcgacggcc ccgtgctgct
     2581 gcccgacaac cactacctga gcacccagtc cgccctgagc aaagacccca acgagaagcg
     2641 cgatcacatg gtcctgctgg agttcgtgac cgccgccggg atcactctcg gcatggacga
     2701 gctgtacaag ggttcaggct cgggatccgg gaattcgatg ggagatacag tagtggagcc
     2761 tgcccccttg aagccaactt ctgagcccac ttctggccca ccagggaata atggggggtc
     2821 cctgctaagt gtcatcacgg agggggtcgg ggaactatca gtgattgacc ctgaggtggc
     2881 ccagaaggcc tgccaggagg tgttggagaa agtcaagctt ttgcatggag gcgtggcagt
     2941 ctctagcaga ggcaccccac tggagttggt caatggggat ggtgtggaca gtgagatccg
     3001 ttgcctagat gatccacctg cccagatcag ggaggaggaa gatgagatgg gggccgctgt
     3061 ggcctcaggc acagccaaag gagcaagaag acggcggcag aacaactcag ctaaacagtc
     3121 ttggctgctg aggctgtttg agtcaaaact gtttgacatc tccatggcca tttcatacct
     3181 gtataactcc aaggagcctg gagtacaagc ctacattggc aaccggctct tctgctttcg
     3241 caacgaggac gtggacttct atctgcccca gttgcttaac atgtacatcc acatggatga
     3301 ggacgtgggt gatgccatta agccctacat agtccaccgt tgccgccaga gcattaactt
     3361 ttccctccag tgtgccctgt tgcttggggc ctattcttca gacatgcaca tttccactca
     3421 acgacactcc cgtgggacca agctacggaa gctgatcctc tcagatgagc taaagccagc
     3481 tcacaggaag agggagctgc cctccttgag cccggcccct gatacagggc tgtctccctc
     3541 caaaaggact caccagcgct ctaagtcaga tgccactgcc agcataagtc tcagcagcaa
     3601 cctgaaacga acagccagca accctaaagt ggagaatgag gatgagcctg ttcgactggc
     3661 tcctgagaga gaattcatca agtccctgat ggcgatcggc aagcggctgg ccacgctccc
     3721 caccaaagag cagaaaacac agaggctgat ctcagagctc tccctgctca accataagct
     3781 ccctgcccga gtctggctgc ccactgctgg ctttgaccac cacgtggtcc gtgtacccca
     3841 cacacaggct gttgtcctca actccaagga caaggctccc tacctgattt atgtggaagt
     3901 ccttgaatgt gaaaactttg acaccaccag tgtccctgcc cggatccccg agaaccgaat
     3961 tcggagtacg aggtccgtag aaaacttgcc cgaatgtggt attacccatg agcagcgagc
     4021 tggcagcttc agcactgtgc ccaactatga caacgatgat gaggcctggt cggtggatga
     4081 cataggcgag ctgcaagtgg agctccccga agtgcatacc aacagctgtg acaacatctc
     4141 ccagttctct gtggacagca tcaccagcca ggagagcaag gagcctgtgt tcattgcagc
     4201 aggggacatc cgccggcgcc tttcggaaca gctggctcat accccgacag ccttcaaacg
     4261 agacccagaa gatccttctg cagttgctct caaagagccc tggcaggaga aagtacggcg
     4321 gatcagagag ggctccccct acggccatct ccccaattgg cggctcctgt cagtcattgt
     4381 caagtgtggg gatgaccttc ggcaagagct tctggccttt caggtgttga agcaactgca
     4441 gtccatttgg gaacaggagc gagtgcccct ttggatcaag ccatacaaga ttcttgtgat
     4501 ttcggctgat agtggcatga ttgaaccagt ggtcaatgct gtgtccatcc atcaggtgaa
     4561 gaaacagtca cagctctcct tgctcgatta cttcctacag gagcacggca gttacaccac
     4621 tgaggcattc ctcagtgcac agcgcaattt tgtgcaaagt tgtgctgggt actgcttggt
     4681 ctgctacctg ctgcaagtca aggacagaca caatgggaat atccttttgg acgcagaagg
     4741 ccacatcatc cacatcgact ttggcttcat cctctccagc tcaccccgaa atctgggctt
     4801 tgagacgtca gcctttaagc tgaccacaga gtttgtggat gtgatgggcg gcctggatgg
     4861 cgacatgttc aactactata agatgctgat gctgcaaggg ctgattgccg ctcggaaaca
     4921 catggacaag gtggtgcaga tcgtggagat catgcagcaa ggttctcagc ttccttgctt
     4981 ccatggctcc agcaccattc gaaacctcaa agagaggttc cacatgagca tgactgagga
     5041 gcagctgcag ctgctggtgg agcagatggt ggatggcagt atgcggtcta tcaccaccaa
     5101 actctatgac ggcttccagt acctcaccaa cggcatcatg tgactcgaga gcggccgcta
     5161 cgtaaattcc gccccccccc ctaacgttac tggccgaagc cgcttggaat aaggccggtg
     5221 tgcgtttgtc tatatgttat tttccaccat attgccgtct tttggcaatg tgagggcccg
     5281 gaaacctggc cctgtcttct tgacgagcat tcctaggggt ctttcccctc tcgccaaagg
     5341 aatgcaaggt ctgttgaatg tcgtgaagga agcagttcct ctggaagttt cttgaagaca
     5401 aacaacgtct gtagcgaccc tttgcaggca gcggaacccc ccacctggcg acaggtgcct
     5461 ctgcggccaa aagccacgtg tataagatac acctgcaaag gcggcacaac cccagtgcca
     5521 cgttgtgagt tggatagttg tggaaagagt caaatggctc tcctcaagcg tattcaacaa
     5581 ggggctgaag gatgcccaga aggtacccca ttgtatggga tctgatctgg ggcctcggtg
     5641 cacatgcttt acatgtgttt agtcgaggtt aaaaaaacgt ctaggccccc cgaaccacgg
     5701 ggacgtggtt ttcctttgaa aaacacgatg ataatatggc cacaaccgtc gacatgaccg
     5761 agtacaagcc cacggtgcgc ctcgccaccc gcgacgacgt cccccgggcc gtacgcaccc
     5821 tcgccgccgc gttcgccgac taccccgcca cgcgccacac cgtcgacccg gaccgccaca
     5881 tcgagcgggt caccgagctg caagaactct tcctcacgcg cgtcgggctc gacatcggca
     5941 aggtgtgggt cgcggacgac ggcgccgcgg tggcggtctg gaccacgccg gagagcgtcg
     6001 aagcgggggc ggtgttcgcc gagatcggcc cgcgcatggc cgagttgagc ggttcccggc
     6061 tggccgcgca gcaacagatg gaaggcctcc tggcgccgca ccggcccaag gagcccgcgt
     6121 ggttcctggc caccgtcggc gtctcgcccg accaccaggg caagggtctg ggcagcgccg
     6181 tcgtgctccc cggagtggag gcggccgagc gcgccggggt gcccgccttc ctggagacct
     6241 ccgcgccccg caacctcccc ttctacgagc ggctcggctt caccgtcacc gccgacgtcg
     6301 aggtgcccga aggaccgcgc acctggtgca tgacccgcaa gcccggtgcc tgagtcgacc
     6361 gcccgcccca cgacccgcag cgcccgaccg aaaggagcgc acgaccccat ggctcgacga
     6421 taaaataaaa gattttattt agtctccaga aaaagggggg aatgaaagac cccacctgta
     6481 ggtttggcaa gctagcttaa gtaacgccat tttgcaaggc atggaaaaat acataactga
     6541 gaatagagaa gttcagatca aggtcaggaa cagatggaac agctgaatat gggccaaaca
     6601 ggatatctgt ggtaagcagt tcctgccccg gctcagggcc aagaacagat ggaacagctg
     6661 aatatgggcc aaacaggata tctgtggtaa gcagttcctg ccccggctca gggccaagaa
     6721 cagatggtcc ccagatgcgg tccagccctc agcagtttct agagaaccat cagatgtttc
     6781 cagggtgccc caaggacctg aaatgaccct gtgccttatt tgaactaacc aatcagttcg
     6841 cttctcgctt ctgttcgcgc gcttctgctc cccgagctca ataaaagagc ccacaacccc
     6901 tcactcggcg cgccagtcct ccgattgact gagtcgcccg ggtacccgtg tatccaataa
     6961 accctcttgc agttgcatcc gacttgtggt ctcgctgttc cttgggaggg tctcctctga
     7021 gtgattgact acccgtcagc gggggtcttt cacatgcagc atgtatcaaa attaatttgg
     7081 ttttttttct taagtattta cattaaatgg ccatagttgc attaatgaat cggccaacgc
     7141 gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg
     7201 cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta
     7261 tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc
     7321 aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag
     7381 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac
     7441 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc
     7501 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt
     7561 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc
     7621 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga
     7681 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta
     7741 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta
     7801 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga
     7861 tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg
     7921 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag
     7981 tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc
     8041 tagatccttt tgcggccggc cgcaaatcaa tctaaagtat atatgagtaa acttggtctg
     8101 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat
     8161 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg
     8221 gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa
     8281 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca
     8341 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc
     8401 gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt
     8461 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa
     8521 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat
     8581 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct
     8641 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga
     8701 gttgctcttg cccggcgtca acacgggata ataccgcgcc acatagcaga actttaaaag
     8761 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga
     8821 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca
     8881 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg
     8941 cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc
     9001 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag
     9061 gggttccgcg cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc attattatca
     9121 tgacattaac ctataaaaat aggcgtatca cgaggccctt tcgtcttcac tataggagct
     9181 tggcccattg catacgttgt atccatatca taatatgtac atttatattg gctcatgtcc
     9241 aacattaccg ccatgtt
//
