LOCUS       sequence-443448-        9626 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  His-TEV-Cas12aUltra-BPSV40_cMyc_NP_5NLS.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9626)
  AUTHORS   Sahu SU, Castro M, Muldoon JJ, Asija K, Wyman SK, Krishnappa N, 
            Eyquem J, Nguyen DN, Wilson RC
  TITLE     Peptide-enabled ribonucleoprotein delivery for CRISPR engineering 
            (PERC) in primary human immune cells and hematopoietic stem cells
  JOURNAL   bioRxiv 2024.07.14.603391
REFERENCE   2  (bases 1 to 9626)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2024.07.14.603391"
COMMENT     Sequence Label: Cas12aUltra_5XNLS
FEATURES             Location/Qualifiers
     source          1..9626
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     489..508
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     742..759
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    774..913
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(839..858)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     999..1021
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(1015..1206)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     CDS             complement(2015..3097)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     3059..3078
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(3098..3175)
                     /gene="lacI"
                     /label=lacI promoter
     primer_bind     3381..3400
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     3484..3503
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        3484..3502
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    3503..3527
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             3542..3564
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             3608..3628
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             3635..7552
                     /codon_start=1
                     /product="CRISPR-associated protein from Acidaminococcus 
                     sp. BV3L6 (Zetsche et al., 2015)"
                     /label=AsCpf1
                     /translation="TQFEGFTNLYQVSKTLRFELIPQGKTLKHIQEQGFIEEDKARNDH
                     YKELKPIIDRIYKTYADQCLQLVQLDWENLSAAIDSYRKEKTEETRNALIEEQATYRNA
                     IHDYFIGRTDNLTDAINKRHAEIYKGLFKAELFNGKVLKQLGTVTTTEHENALLRSFDK
                     FTTYFSGFYENRKNVFSAEDISTAIPHRIVQDNFPKFKENCHIFTRLITAVPSLREHFE
                     NVKKAIGIFVSTSIEEVFSFPFYNQLLTQTQIDLYNQLLGGISREAGTEKIKGLNEVLN
                     LAIQKNDETAHIIASLPHRFIPLFKQILSDRNTLSFILEEFKSDEEVIQSFCKYKTLLR
                     NENVLETAEALFNELNSIDLTHIFISHKKLETISSALCDHWDTLRNALYERRISELTGK
                     ITKSAKEKVQRSLKHEDINLQEIISAAGKELSEAFKQKTSEILSHAHAALDQPLPTTLK
                     KQEEKEILKSQLDSLLGLYHLLDWFAVDESNEVDPEFSARLTGIKLEMEPSLSFYNKAR
                     NYATKKPYSVEKFKLNFQRPTLASGWDVNKEKNNGAILFVKNGLYYLGIMPKQKGRYKA
                     LSFEPTEKTSEGFDKMYYDYFPDAAKMIPKCSTQLKAVTAHFQTHTTPILLSNNFIEPL
                     EITKEIYDLNNPEKEPKKFQTAYAKKTGDQKGYREALCKWIDFTRDFLSKYTKTTSIDL
                     SSLRPSSQYKDLGEYYAELNPLLYHISFQRIAEKEIMDAVETGKLYLFQIYNKDFAKGH
                     HGKPNLHTLYWTGLFSPENLAKTSIKLNGQAELFYRPKSRMKRMAHRLGEKMLNKKLKD
                     QKTPIPDTLYQELYDYVNHRLSHDLSDEARALLPNVITKEVSHEIIKDRRFTSDKFLFH
                     VPITLNYQAANSPSKFNQRVNAYLKEHPETPIIGIDRGERNLIYITVIDSTGKILEQRS
                     LNTIQQFDYQKKLDNREKERVAARQAWSVVGTIKDLKQGYLSQVIHEIVDLMIHYQAVV
                     VLENLNFGFKSKRTGIAEKAVYQQFEKMLIDKLNCLVLKDYPAEKVGGVLNPYQLTDQF
                     TSFAKMGTQSGFLFYVPAPYTSKIDPLTGFVDPFVWKTIKNHESRKHFLEGFDFLHYDV
                     KTGDFILHFKMNRNLSFQRGLPGFMPAWDIVFEKNETQFDAKGTPFIAGKRIVPVIENH
                     RFTGRYRDLYPANELIALLEEKGIVFRDGSNILPKLLENDDSHAIDTMVALIRSVLQMR
                     NSNAATGEDYINSPVRDLNGVCFDSRFQNPEWPMDADANGAYHIALKGQLLLNHLKESK
                     DLKLQNGISNQDWLAYIQELRN"
     regulatory      7272..7281
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             7553..7600
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             7607..7687
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAYPYDVPDYAYPYDVPDYA"
     CDS             7688..7714
                     /codon_start=1
                     /product="nuclear localization signal (Makkerh et al., 
                     1996)"
                     /label=NLS
                     /translation="PAAKKKKLD"
     CDS             7721..7747
                     /codon_start=1
                     /product="nuclear localization signal (Makkerh et al., 
                     1996)"
                     /label=NLS
                     /translation="PAAKKKKLD"
     CDS             7787..7807
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             7814..7834
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             7841..7858
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(7911..7929)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      7925..7972
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      8009..8464
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8096..8115)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8306..8327
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        8490..8594
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8595..8663,8664..9455)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8813..8832)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      join(9626,1..588)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
       61 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
      121 agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc
      181 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
      241 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
      301 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
      361 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
      421 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
      481 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
      541 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
      601 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
      661 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
      721 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg
      781 cggtattttc tccttacgca tctgtgcggt atttcacacc gcaatggtgc actctcagta
      841 caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg
      901 ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct
      961 gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag
     1021 gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc
     1081 gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag
     1141 aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt
     1201 ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa
     1261 acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg
     1321 ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg
     1381 tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc
     1441 tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta
     1501 cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca
     1561 gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc
     1621 ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggggccgc
     1681 catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa
     1741 ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc
     1801 gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac
     1861 gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca
     1921 ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgagatc ccggtgccta
     1981 atgagtgagc taacttacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa
     2041 cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat
     2101 tgggcgccag ggtggttttt cttttcacca gtgagacggg caacagctga ttgcccttca
     2161 ccgcctggcc ctgagagagt tgcagcaagc ggtccacgct ggtttgcccc agcaggcgaa
     2221 aatcctgttt gatggtggtt aacggcggga tataacatga gctgtcttcg gtatcgtcgt
     2281 atcccactac cgagatatcc gcaccaacgc gcagcccgga ctcggtaatg gcgcgcattg
     2341 cgcccagcgc catctgatcg ttggcaacca gcatcgcagt gggaacgatg ccctcattca
     2401 gcatttgcat ggtttgttga aaaccggaca tggcactcca gtcgccttcc cgttccgcta
     2461 tcggctgaat ttgattgcga gtgagatatt tatgccagcc agccagacgc agacgcgccg
     2521 agacagaact taatgggccc gctaacagcg cgatttgctg gtgacccaat gcgaccagat
     2581 gctccacgcc cagtcgcgta ccgtcttcat gggagaaaat aatactgttg atgggtgtct
     2641 ggtcagagac atcaagaaat aacgccggaa cattagtgca ggcagcttcc acagcaatgg
     2701 catcctggtc atccagcgga tagttaatga tcagcccact gacgcgttgc gcgagaagat
     2761 tgtgcaccgc cgctttacag gcttcgacgc cgcttcgttc taccatcgac accaccacgc
     2821 tggcacccag ttgatcggcg cgagatttaa tcgccgcgac aatttgcgac ggcgcgtgca
     2881 gggccagact ggaggtggca acgccaatca gcaacgactg tttgcccgcc agttgttgtg
     2941 ccacgcggtt gggaatgtaa ttcagctccg ccatcgccgc ttccactttt tcccgcgttt
     3001 tcgcagaaac gtggctggcc tggttcacca cgcgggaaac ggtctgataa gagacaccgg
     3061 catactctgc gacatcgtat aacgttactg gtttcacatt caccaccctg aattgactct
     3121 cttccgggcg ctatcatgcc ataccgcgaa aggttttgcg ccattcgatg gtgtccggga
     3181 tctcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg
     3241 ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc
     3301 ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg
     3361 cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg
     3421 gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga
     3481 aattaatacg actcactata ggggaattgt gagcggataa caattcccct ctagaaataa
     3541 ttttgtttaa ctttaagaag gagatataca tatgaaacgt accgccgacg gcagcgagtt
     3601 cgagagcccg aaaaagaaac gcaaagtggg atcaacacag ttcgagggct ttaccaacct
     3661 gtatcaggtg agcaagacac tgcggtttga gctgatccca cagggcaaga ccctgaagca
     3721 catccaggag cagggcttca tcgaggagga caaggcccgc aatgatcact acaaggagct
     3781 gaagcccatc atcgatcgga tctacaagac ctatgccgac cagtgcctgc agctggtgca
     3841 gctggattgg gagaacctga gcgccgccat cgactcctat agaaaggaga aaaccgagga
     3901 gacaaggaac gccctgatcg aggagcaggc cacatatcgc aatgccatcc acgactactt
     3961 catcggccgg acagacaacc tgaccgatgc catcaataag agacacgccg agatctacaa
     4021 gggcctgttc aaggccgagc tgtttaatgg caaggtgctg aagcagctgg gcaccgtgac
     4081 cacaaccgag cacgagaacg ccctgctgcg gagcttcgac aagtttacaa cctacttctc
     4141 cggcttttat gagaacagga agaacgtgtt cagcgccgag gatatcagca cagccatccc
     4201 acaccgcatc gtgcaggaca acttccccaa gtttaaggag aattgtcaca tcttcacacg
     4261 cctgatcacc gccgtgccca gcctgcggga gcactttgag aacgtgaaga aggccatcgg
     4321 catcttcgtg agcacctcca tcgaggaggt gttttccttc cctttttata accagctgct
     4381 gacacagacc cagatcgacc tgtataacca gctgctggga ggaatctctc gggaggcagg
     4441 caccgagaag atcaagggcc tgaacgaggt gctgaatctg gccatccaga agaatgatga
     4501 gacagcccac atcatcgcct ccctgccaca cagattcatc cccctgttta agcagatcct
     4561 gtccgatagg aacaccctgt ctttcatcct ggaggagttt aagagcgacg aggaagtgat
     4621 ccagtccttc tgcaagtaca agacactgct gagaaacgag aacgtgctgg agacagccga
     4681 ggccctgttt aacgagctga acagcatcga cctgacacac atcttcatca gccacaagaa
     4741 gctggagaca atcagcagcg ccctgtgcga ccactgggat acactgagga atgccctgta
     4801 tgagcggaga atctccgagc tgacaggcaa gatcaccaag tctgccaagg agaaggtgca
     4861 gcgcagcctg aagcacgagg atatcaacct gcaggagatc atctctgccg caggcaagga
     4921 gctgagcgag gccttcaagc agaaaaccag cgagatcctg tcccacgcac acgccgccct
     4981 ggatcagcca ctgcctacaa ccctgaagaa gcaggaggag aaggagatcc tgaagtctca
     5041 gctggacagc ctgctgggcc tgtaccacct gctggactgg tttgccgtgg atgagtccaa
     5101 cgaggtggac cccgagttct ctgcccggct gaccggcatc aagctggaga tggagccttc
     5161 tctgagcttc tacaacaagg ccagaaatta tgccaccaag aagccctact ccgtggagaa
     5221 gttcaagctg aactttcagc ggcctacact ggcctctggc tgggacgtga ataaggagaa
     5281 gaacaatggc gccatcctgt ttgtgaagaa cggcctgtac tatctgggca tcatgccaaa
     5341 gcagaagggc aggtataagg ccctgagctt cgagcccaca gagaaaacca gcgagggctt
     5401 tgataagatg tactatgact acttccctga tgccgccaag atgatcccaa agtgcagcac
     5461 ccagctgaag gccgtgacag cccactttca gacccacaca acccccatcc tgctgtccaa
     5521 caatttcatc gagcctctgg agatcacaaa ggagatctac gacctgaaca atcctgagaa
     5581 ggagccaaag aagtttcaga cagcctacgc caagaaaacc ggcgaccaga agggctacag
     5641 agaggccctg tgcaagtgga tcgacttcac aagggatttt ctgtccaagt ataccaagac
     5701 aacctctatc gatctgtcta gcctgcggcc atcctctcag tataaggacc tgggcgagta
     5761 ctatgccgag ctgaatcccc tgctgtacca catcagcttc cagagaatcg ccgagaagga
     5821 gatcatggat gccgtggaga caggcaagct gtacctgttc cagatctata acaaggactt
     5881 tgccaagggc caccacggca agcctaatct gcacacactg tattggaccg gcctgttttc
     5941 tccagagaac ctggccaaga caagcatcaa gctgaatggc caggccgagc tgttctaccg
     6001 ccctaagtcc aggatgaaga ggatggcaca ccggctggga gagaagatgc tgaacaagaa
     6061 gctgaaggat cagaaaaccc caatccccga caccctgtac caggagctgt acgactatgt
     6121 gaatcacaga ctgtcccacg acctgtctga tgaggccagg gccctgctgc ccaacgtgat
     6181 caccaaggag gtgtctcacg agatcatcaa ggataggcgc tttaccagcg acaagttcct
     6241 tttccacgtg cctatcacac tgaactatca ggccgccaat tccccatcta agttcaacca
     6301 gagggtgaat gcctacctga aggagcaccc cgagacacct atcatcggca tcgatcgggg
     6361 cgagagaaac ctgatctata tcacagtgat cgactccacc ggcaagatcc tggagcagcg
     6421 gagcctgaac accatccagc agtttgatta ccagaagaag ctggacaaca gggagaagga
     6481 gagggtggca gcaaggcagg cctggtctgt ggtgggcaca atcaaggatc tgaagcaggg
     6541 ctatctgagc caggtcatcc acgagatcgt ggacctgatg atccactacc aggccgtggt
     6601 ggtgctggag aacctgaatt tcggctttaa gagcaagagg accggcatcg ccgagaaggc
     6661 cgtgtaccag cagttcgaga agatgctgat cgataagctg aattgcctgg tgctgaagga
     6721 ctatccagca gagaaagtgg gaggcgtgct gaacccatac cagctgacag accagttcac
     6781 ctcctttgcc aagatgggca cccagtctgg cttcctgttt tacgtgcctg ccccatatac
     6841 atctaagatc gatcccctga ccggcttcgt ggaccccttc gtgtggaaaa ccatcaagaa
     6901 tcacgagagc cgcaagcact tcctggaggg cttcgacttt ctgcactacg acgtgaaaac
     6961 cggcgacttc atcctgcact ttaagatgaa cagaaatctg tccttccaga ggggcctgcc
     7021 cggctttatg cctgcatggg atatcgtgtt cgagaagaac gagacacagt ttgacgccaa
     7081 gggcacccct ttcatcgccg gcaagagaat cgtgccagtg atcgagaatc acagattcac
     7141 cggcagatac cgggacctgt atcctgccaa cgagctgatc gccctgctgg aggagaaggg
     7201 catcgtgttc agggatggct ccaacatcct gccaaagctg ctggagaatg acgattctca
     7261 cgccatcgac accatggtgg ccctgatccg cagcgtgctg cagatgcgga actccaatgc
     7321 cgccacaggc gaggactata tcaacagccc cgtgcgcgat ctgaatggcg tgtgcttcga
     7381 ctcccggttt cagaacccag agtggcccat ggacgccgat gccaatggcg cctaccacat
     7441 cgccctgaag ggccagctgc tgctgaatca cctgaaggag agcaaggatc tgaagctgca
     7501 gaacggcatc tccaatcagg actggctggc ctacatccag gagctgcgca acaaaaggcc
     7561 ggcggccacg aaaaaggccg gccaggcaaa aaagaaaaag ggatcctacc catacgatgt
     7621 tccagattac gcttatccct acgacgtgcc tgattatgca tacccatatg atgtccccga
     7681 ctatgccccg gcagctaaga agaagaaact ggatggcagc cccgccgcca agaagaagaa
     7741 gctggacggc agcaaacgta ccgccgacgg cagcgagttc gagagcccga aaaagaaacg
     7801 caaagtgggt tcggagaatt tgtattttca gggtctcgag caccaccacc accaccactg
     7861 agatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca
     7921 ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg
     7981 aggaactata tccggattgg cgaatgggac gcgccctgta gcggcgcatt aagcgcggcg
     8041 ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct
     8101 ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat
     8161 cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt
     8221 gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg
     8281 acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac
     8341 cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta
     8401 aaaaatgagc tgatttaaca aaaatttaac gcgaatttta acaaaatatt aacgcttaca
     8461 atttaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa
     8521 tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt
     8581 gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg
     8641 cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag
     8701 atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg
     8761 agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg
     8821 gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt
     8881 ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga
     8941 cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac
     9001 ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc
     9061 atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc
     9121 gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac
     9181 tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag
     9241 gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg
     9301 gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta
     9361 tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg
     9421 ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata
     9481 tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt
     9541 ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc
     9601 ccgtagaaaa gatcaaagga tcttct
//
