LOCUS       sequence-44345-e         830 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 830)
  AUTHORS   Chen ZX, Mann JR, Hsieh CL, Riggs AD, Chedin F
  TITLE     Physical and functional interactions between the human DNMT3L 
            protein and members of the de novo methyltransferase family.
  JOURNAL   J Cell Biochem. 2005 Aug 1;95(5):902-17.
  PUBMED    15861382
REFERENCE   2  (bases 1 to 830)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biochem."; date: "2005-08-1"; volume: "95(5)"; pages: "902-17"
COMMENT     Sequence Label: pcDNA3/Myc-DNMT3L
FEATURES             Location/Qualifiers
     source          1..830
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     49..68
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        49..67
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      81..90
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             90..119
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
ORIGIN
        1 agctctctgg ctaactagag aacccactgc ttactggctt atcgaaatta atacgactca
       61 ctatagggag acccaagctt gccaccatgg agcagaagct gatctcagag gaggacctgg
      121 aattcatggc ggccatccca gccctggacc cagaggccga gcccagcatg gacgtgattt
      181 tggtgggatc cagtgagctc tcaagctccg tttcacccgg gacaggcaga gatcttattg
      241 catatgaagt caaggctaac cagcgaaata tagaagacat ctgcatctgc tgcggaagtc
      301 tccaggttca cacacagcac cctctgtttg agggagggat ctgcgcccca tgtaaggaca
      361 agttcctgga tgccctcttc ctgtacgacg atgacgggta ccaatcctac tgctccatct
      421 gctgctccgg agagacgctg ctcatctgcg gaaaccctga ttgcacccga tgctactgct
      481 tcgagtgtgt ggatagcctg gtcggccccg ggacctcggg gaaggtgcac gccatgagca
      541 actgggtgtg ctacctgtgc ctgccgtcct cccgaagcgg gctgctgcag cgtcggagga
      601 agtggcgcag ccagctcaag gccttctacg accgagagtc ggagaatccc cttgagatgt
      661 tcgaaaccgt gcctgtgtgg aggagacagc cagtccgggt gctgtccctt tttgaagaca
      721 tcaagaaaga gctgacgagt ttgggctttt tggaaagtgg ttctgacccg ggacaactga
      781 agcatgtggt tgatgtcaca gacacagtga ggaaggatgt ggaggagtgg
//
