LOCUS       sequence-443450-       10339 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  TEF1(p)-mNeonGreen:amdS fusion reporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10339)
  AUTHORS   Phillips-Rose LS, Yu CK, West NP, Fraser JA
  TITLE     A Chimeric ORF Fusion Phenotypic Reporter for Cryptococcus 
            neoformans.
  JOURNAL   J Fungi (Basel). 2024 Aug 12;10(8):567. doi: 10.3390/jof10080567.
  PUBMED    39194893
REFERENCE   2  (bases 1 to 10339)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Fungi 
            (Basel)."; date: "2024-08-12"; volume: "10(8):567. doi"; pages: " 
            10.3390/jof10080567"
COMMENT     Sequence Label: pLSPR33
FEATURES             Location/Qualifiers
     source          1..10339
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(1823..2623)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MSMIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQG
                     RPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL
                     LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEE
                     HQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQD
                     IALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(1934..1953)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     2544..2563
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     3713..3735
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3727..3744
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3728..3744
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3754..3773
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        3754..3772
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      5278..5287
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(8150..8170)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(8150..8168)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(8189..8205)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8189..8205)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8202..8224)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8213..8229
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8237..8243,8244..8261,8262..8267))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8282..8303
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8420..8437)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8591..9179)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8671..8690)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9350..10141,10142..10210))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9973..9992
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10211..10315)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 tgacgcgccc tgtagcggca agatctgccc atagacttca aatcatggaa ttggacgttt
       61 atcttcttgc tttggatatc caccctatcg tacaatcttg gcactttgga tcatacgagg
      121 cactttgcgg actagggaat ggttatcatc aatttcttgg acctctccat gacacctggc
      181 atcgtcgagc taccacagcc ccctgaattc ttaaaggcga accccccttc ctaggaacct
      241 tgagcgctgg actggatggc actctgagac cgcctatgct taaggacgcg agcttaccct
      301 tccattgatg gaagtcgatc atttatgata atagaacacg gcggaacttg actttacagg
      361 ccaagggaag caacaggaga agaagctaag gttataacag gctcagcata tttgtatata
      421 cggtgccatt caacgctgaa cactttgtac tcacggtctg cccgttgaat aacaccatct
      481 gctgatgatc atcccaattt gtgagtctta tttgccattg atcctggtgt ataaccgata
      541 ctgaagccct ttaggtctga tcagttatag acgtggtata ttcattcttc ctagattagc
      601 tatgccgaaa gagatggata ccttgatgtg actggaatgc catgaagcag gagattgtag
      661 cagctcaaat tggagaagga tgttgcaacc gtcggccgtg aaggtcttgt catatctttg
      721 tctgcctggg agaatatgta caagtgcccc gatgttactg atggaaaaca tcgtgtggcg
      781 cgccacgata cttgtgttaa ttaaaccaaa ccagtctgtg ccaagtggat tgcttatgca
      841 tcgagtggcg agcccactga cctctgggcc ccttttttga actataaaaa gcggccaagt
      901 ctgatttcgg tctcacaatc atcaacatcg acttctcttt tcgcctcgat caacctaaac
      961 aacggtcact cagccatgaa ctggacaatg gaatatttcg ccttgccttg gatctttggc
     1021 ctctagactc aatcaacgtc aggataaagg tgagcatagg ttgctcactc actgtagcct
     1081 ttttttccct tcttcttggt ccagatggtt cccaaggctc tataaagtgt atctgtcagt
     1141 tccacttcct tactccgaag cgattcagag ttgtaaagtg cgtctgaccc cctgattata
     1201 tctcaaagtc ccttcacaaa ttcagacatg atgcgataaa gtgccggatt gtcattcgcg
     1261 tcttgtgact gtatagaaag tgccggcatc ctcttttctt ttcctctttg gccgagcgac
     1321 tcagagttgt gaagcctgct ggtatctgtc tgttcccttg ccctgataaa gaatatcgaa
     1381 gagcatttct ttgattcaca atacgttgac gaatggtgtg atggatccta agcagcgcca
     1441 gccctttgaa cttgtgtcga aaaaaaaaaa aaaacagcat atgatacgta aagggaagaa
     1501 aaggatggag ctgactgttt tgacaggcat aattttgagc gtgcttcatt ggccggttta
     1561 tctgtattaa cacggaagag atgtagaaac tagcttcctg gtttcagaga caaggagcca
     1621 tgaagatcct gaggattcaa cggcgttgct gagggtggga aggatggtat tgttgtctgt
     1681 agatagatta atgggcatat aatgtcaaaa aagatgattg acacgatgtc tgtctcgaat
     1741 tcgcagtgtg ctaaaattgt atcacttctc taatatgtat atatacaccc tctaaggaaa
     1801 attaacccct taccgccttc actcagaaga actcgtcaag aaggcgatag aaggcgatgc
     1861 gctgcgaatc gggagcggcg ataccgtaaa gcacgaggaa gcggtcagcc cattcgccgc
     1921 caagctcttc agcaatatca cgggtagcca acgctatgtc ctgatagcgg tccgccacac
     1981 ccagccggcc acagtcgatg aatccagaaa agcggccatt ttccaccatg atattcggca
     2041 agcaggcatc gccatgggtc acgacgagat cctcgccgtc gggcatgcgc gccttgagcc
     2101 tggcgaacag ttcggctggc gcgagcccct gatgctcttc gtccagatca tcctgatcga
     2161 caagaccggc ttccatccga gtacgtgctc gctcgatgcg atgtttcgct tggtggtcga
     2221 atgggcaggt agccggatca agcgtatgca gccgccgcat tgcatcagcc atgatggata
     2281 ctttctcggc aggagcaagg tgggatgaca ggagatcctg ccccggcact tcgcccaata
     2341 gcagccagtc ccttcccgct tcagtgacaa cgtcgagcac agctgcgcaa ggaacgcccg
     2401 tcgtggccag ccacgatagc cgcgctgcct cgtcctgcag ttcattcagg gcaccggaca
     2461 ggtcggtctt gacaaaaaga accgggcgcc cctgcgctga cagccggaac acggcggcat
     2521 cagagcagcc gattgtctgt tgtgcccagt catagccgaa tagcctctcc acccaagcgg
     2581 ccggagaacc tgcgtgcaat ccatcttgtt caatcataga catgttgggc gagtttacta
     2641 atggaaaaag acaattagca aagaggctgc atattaggag acgcggtgaa gattggggag
     2701 gacaataggg tccgtgcacc aaaaagtcct gacggcagct ctgttcaacg cacatccatc
     2761 tcttccagct atgcgtgtgg caccagcacg gatggattgt tgcaccaaaa ggacaaggcg
     2821 agatggatgc aggtcgaggc gatgcggtgc tatcggccct cgttgtcgaa cgccacgtcg
     2881 tcctctcggc gaccgtcaat cctggaagga aatgaccaag cgtgcaccca gcatccagca
     2941 tccaacatcc agcacccagc acctagcacc catgatccaa tggccggcat gcagatcagc
     3001 gtggggagag caccatctag cagcccctgg caggcccagc attgtgctcg gatgtgcgat
     3061 aggcggtggg tgcgataagg tggattagtg gaggatggag gactgagtgg cgggcaaggg
     3121 aagggggcgg gaagatgact caccttgggc agtgggtggg ggatgcggta taggggatgt
     3181 atgtggatgc tggcggagga tagaagctgt aagctgagct ggtgccaacg gtgacgctgt
     3241 gagagtggtc tcggcgaccc tgacggcgac tcggcgttac cccgtgtggc acaccggacg
     3301 cgcgtcgagt gacgttgctg ctattgttgt taccatcatc ctctcctccg acaaccatac
     3361 actcagtgca caccatgcca ctcgaatcct gcatgcttat gtgagtcctc cccttcccgt
     3421 gcgccgctct ccagctcaca tcctcgcagc catggactca accctatctc ggtctattct
     3481 tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaaatg agctgattta
     3541 acaaaaattt aacgcgaatt ttaacaaaat attaacgctt acaatttcca ttcgccattc
     3601 aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg
     3661 gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca
     3721 cgacgttgta aaacgacggc cagtgagcgc gcgtaatacg actcactata gggcgaattg
     3781 ggtaccgggc cccccctcga cattctatgc tatacggtac aagattgtgg ctactatgca
     3841 agctccatag cgaatgatct tacactgacc ttgtcgaccg ttctgaatga ttaacaatgg
     3901 tcgcaagtgt ggttgaccca cctgcgggcg cttcttaact atctatcatc ggtatgtcaa
     3961 gtatcactcc ctttaaacaa aagaataaga gcataataca gcagtctcat catggcaacc
     4021 cttcgcgcaa tgtacaaaaa gcagccagaa gcccccacgc acaagcaata atgtcgtgtt
     4081 ctaccctgcc atgaacaagc gtcatggcca ttagcgaacg ccccaatatt cagccccatt
     4141 cgccaagcaa gttgcctcgc ccccttccca gaagatcatg ggcatctcgt cttcttctaa
     4201 tttcgcattt tgtgaagctc cattcatgca cgtcggcttc tggacgactc ttatcatatg
     4261 cattggcctg ctcggtgtga ggatgagcca ctcccacatg tttagacggc aggggcctct
     4321 tgccacccgt tgaatttttg gtgcgaagac gtgcctggga acgacctcca ctttaaattt
     4381 ctccgttgtt gcggtcgctc catcctcctc acatttcttg cctcctaagt cccatgtgcc
     4441 acactcggtg aaaaacggag tttgatacgg atgaaatttc cagtgagctt ttcacattct
     4501 ggaggtttcg aaaaaaattg tgggattttc tgaattaatc cgagaatccg ccctgcccat
     4561 caaccggaaa gaattaaatc cctcgcacat tcctttctcc caccaccttc tttatctcta
     4621 cattctttaa ccaaaacacc taggtgagta cccaaagtct tttgacacct ttgaaaatcc
     4681 gcgccagtcg tagggcacgt aaaacaattc tcgctggtgg cttctgcagg tgaagtcggg
     4741 gcagagtgga gctgagcatg gtcgaccacc cacatcattc tgtcttgttt ttggcgatgt
     4801 cttccatgct tttgccctca tctgttgcgt cgactgcaga gtactgtttt gccactactg
     4861 agctcaacat gggcttgtgt gtggtgaaga aaaaaatttg gtggctcttg aaaattttca
     4921 acccatcgga tacatgccct tccaactcac aacgccctaa cactttattg tcctatcatt
     4981 gcctcttcgc acatgctaac gatctccaca gaaaacttca aaatggtctc caagggcgag
     5041 gaggacaaca tggcctctct ccctgccact cacgagttgc acatcttcgg ctccatcaac
     5101 ggtgtcgact tcgacatggt cggtcagggc accggcaacc ctaacgacgg ttacgaggag
     5161 ttgaacctta agtccaccaa gggtgacctc cagttctccc cctggattct tgtccctcac
     5221 atcggctacg gcttccacca gtaccttccc taccctgacg gcatgtcccc tttccaggcc
     5281 gccatggttg acggctccgg ttaccaggtc caccgtacta tgcagttcga ggacggtgcc
     5341 tcccttactg ttaactaccg ttacacctac gagggttccc acatcaaggg tgaggcccag
     5401 gtcaagggca ctggtttccc tgctgacggt cctgtcatga ccaactccct taccgctgcc
     5461 gactggtgca ggtccaagaa gacttacccc aacgacaaga ccatcatctc caccttcaag
     5521 tggtcctaca ccactggtaa cggcaagcgt taccgatcca ctgcccgaac cacctacacc
     5581 ttcgccaagc ctatggccgc taactacctt aagaaccagc ccatgtacgt tttccgtaag
     5641 accgagctca agcactccaa gaccgagctc aacttcaagg agtggcagaa ggccttcacc
     5701 gacgtcatgg gcatggacga gctttacaag ggttctggcg gtggtggctc cggcatgcct
     5761 cagtcctggg aggagctcgc cgctgacaag cgtgcccgtc tcgctaagac catccctgac
     5821 gagtggaagg tccagaccct ccctgccgag gactccgtta ttgacttccc taagaagtct
     5881 ggcatccttt ctgaggccga gctcaagatc actgaggcct ccgctgctga ccttgtctcc
     5941 aagctcgccg ccggtgagtt gacctctgtc gaggttaccc ttgctttctg taagcgagct
     6001 gctatcgccc agcagttgac taactgcgcc cacgagttct tccctgacgc cgctctcgcc
     6061 caggctaggg agctcgacga gtactacgct aagcacaaga ggcccgttgg tcctctccac
     6121 ggcctcccca tctctctcaa ggaccagctt cgagtcaagg gctacgagac ttctatgggc
     6181 tacatctctt ggcttaacaa gtacgacgag ggcgactctg ttctcactac catgctccgt
     6241 aaggccggtg ccgtcttcta cgtcaagacc tctgtccccc agaccctcat ggtctgcgag
     6301 actgtcaaca acatcatcgg ccgtaccgtc aaccctcgta acaagaactg gtcttgcggc
     6361 ggctcctctg gtggtgaggg tgccatcgtt ggcattcgtg gtggcgtcat cggtgttggt
     6421 accgacattg gtggctctat tcgagtcccc gccgccttca acttcctcta cggtcttagg
     6481 ccctcccacg gccgactccc ctacgctaag atggccaact ccatggaggg tcaggagacc
     6541 gtccactccg ttgtcggccc cattacccac tctgttgagg acctccgtct cttcaccaag
     6601 tccgtcctcg gtcaggagcc ttggaagtac gactccaagg tcatccccat gccctggcgt
     6661 cagtccgagt ctgacattat tgcctccaag atcaagaacg gcggcctcaa catcggctac
     6721 tacaacttcg acggcaacgt ccttcctcac cctcctatcc tccgtggcgt cgagaccacc
     6781 gtcgccgctc tcgccaaggc cggtcacacc gtcaccccct ggacccctta caagcacgac
     6841 ttcggccacg acctcatctc ccacatctac gccgctgacg gctccgccga cgttatgcgt
     6901 gacatctccg cttccggcga gcccgccatt cctaacatca aggaccttct caaccccaac
     6961 atcaaggctg ttaacatgaa cgagctctgg gacacccacc tccagaagtg gaactaccag
     7021 atggagtacc ttgagaagtg gcgagaggct gaggagaagg ccggcaagga ggtgagtaga
     7081 tcacaatttg taacaggttg gatgataagt ccccggtcta acatgtagcg acgacactga
     7141 agctcacttc ttgtagctcg acgccatcat cgcccccatt acccctaccg ctgccgttcg
     7201 acacgaccag ttccgatact acggctacgc ctctgtcatc aacctcctcg acttcacctc
     7261 cgtcgttgtt cccgttacct tcgccgacaa gaacatcgac aagaagaacg agtccttcaa
     7321 ggccgtttcc gagcttgacg ccctcgtcca ggaggagtac gaccccgagg cctaccacgg
     7381 cgctcccgtt gctgtccagg ttatcggtcg aaggctctcc gaggagagga ccttggccat
     7441 tgctgaggag gtcggcaagt tgctcggtaa cgtcgtcact ccttagaagg ttgagcgcgt
     7501 aatagtgggt tggtcttgtc tctatccatt catctgtgag acatactgat ttcaactttt
     7561 tcatcagata ataaggatgg tgtgagataa gcaaggctgg gttatgaggt ttgggactag
     7621 atggtgtttc gcgtctaaag gtgagtcttc gttggttcaa tcacattgat ctgatcgctg
     7681 atcaattgtc agcctctgaa tgtgctcttg aagaaaatgg ttgtctgtaa gaatccagaa
     7741 ccaaaaacgg tcatttcaag tatgtatcct ccgtgaagtt cctttagctc ttgctaatat
     7801 tggactttag cgtgctttag ccaaagacag ggtcgcgagg ttaagaggat gggccaggaa
     7861 aaatctaagt tagatttgtc acacaaagaa aaagattgtc tcagttttgg ttttgtttgg
     7921 tttgatttat gcataggaaa tatttatgat cgaaatcact catttcctgc ttcgtaaata
     7981 tcatctacta tatgcttcgt aaatgtccat ctcctacata ttgtggatat gataaatgca
     8041 atcctctcgt ctacactctc ggcttatgcc tttgtcttct ttgcgctcct tttagccttc
     8101 tttttattcc tagtcttcaa gcccatcggt ggagctccag cttttgttcc ctttagtgag
     8161 ggttaattgc gcgcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc
     8221 cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct
     8281 aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa
     8341 acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta
     8401 ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc
     8461 gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg
     8521 caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt
     8581 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa
     8641 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct
     8701 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc
     8761 cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg
     8821 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct
     8881 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag
     8941 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga
     9001 agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga
     9061 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg
     9121 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag
     9181 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag
     9241 ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat
     9301 gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct
     9361 taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac
     9421 tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa
     9481 tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg
     9541 gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt
     9601 gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca
     9661 ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt
     9721 cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct
     9781 tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg
     9841 cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg
     9901 agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg
     9961 cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa
    10021 aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt
    10081 aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt
    10141 gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt
    10201 gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca
    10261 tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat
    10321 ttccccgaaa agtgccacc
//
