LOCUS       sequence-443452-       10027 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  GAL7(p)-mNeonGreen:amdS fusion reporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10027)
  AUTHORS   Phillips-Rose LS, Yu CK, West NP, Fraser JA
  TITLE     A Chimeric ORF Fusion Phenotypic Reporter for Cryptococcus 
            neoformans.
  JOURNAL   J Fungi (Basel). 2024 Aug 12;10(8):567. doi: 10.3390/jof10080567.
  PUBMED    39194893
REFERENCE   2  (bases 1 to 10027)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Fungi 
            (Basel)."; date: "2024-08-12"; volume: "10(8):567. doi"; pages: " 
            10.3390/jof10080567"
COMMENT     Sequence Label: pLSPR32
FEATURES             Location/Qualifiers
     source          1..10027
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(1823..2623)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MSMIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQG
                     RPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL
                     LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEE
                     HQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQD
                     IALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(1934..1953)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     2544..2563
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     3713..3735
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3727..3744
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3728..3744
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3754..3773
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        3754..3772
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      4966..4975
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(7838..7858)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(7838..7856)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(7877..7893)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7877..7893)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7890..7912)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7901..7917
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7925..7931,7932..7949,7950..7955))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7970..7991
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8108..8125)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8279..8867)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8359..8378)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9038..9829,9830..9898))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9661..9680
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9899..10003)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 tgacgcgccc tgtagcggca agatctgccc atagacttca aatcatggaa ttggacgttt
       61 atcttcttgc tttggatatc caccctatcg tacaatcttg gcactttgga tcatacgagg
      121 cactttgcgg actagggaat ggttatcatc aatttcttgg acctctccat gacacctggc
      181 atcgtcgagc taccacagcc ccctgaattc ttaaaggcga accccccttc ctaggaacct
      241 tgagcgctgg actggatggc actctgagac cgcctatgct taaggacgcg agcttaccct
      301 tccattgatg gaagtcgatc atttatgata atagaacacg gcggaacttg actttacagg
      361 ccaagggaag caacaggaga agaagctaag gttataacag gctcagcata tttgtatata
      421 cggtgccatt caacgctgaa cactttgtac tcacggtctg cccgttgaat aacaccatct
      481 gctgatgatc atcccaattt gtgagtctta tttgccattg atcctggtgt ataaccgata
      541 ctgaagccct ttaggtctga tcagttatag acgtggtata ttcattcttc ctagattagc
      601 tatgccgaaa gagatggata ccttgatgtg actggaatgc catgaagcag gagattgtag
      661 cagctcaaat tggagaagga tgttgcaacc gtcggccgtg aaggtcttgt catatctttg
      721 tctgcctggg agaatatgta caagtgcccc gatgttactg atggaaaaca tcgtgtggcg
      781 cgccacgata cttgtgttaa ttaaaccaaa ccagtctgtg ccaagtggat tgcttatgca
      841 tcgagtggcg agcccactga cctctgggcc ccttttttga actataaaaa gcggccaagt
      901 ctgatttcgg tctcacaatc atcaacatcg acttctcttt tcgcctcgat caacctaaac
      961 aacggtcact cagccatgaa ctggacaatg gaatatttcg ccttgccttg gatctttggc
     1021 ctctagactc aatcaacgtc aggataaagg tgagcatagg ttgctcactc actgtagcct
     1081 ttttttccct tcttcttggt ccagatggtt cccaaggctc tataaagtgt atctgtcagt
     1141 tccacttcct tactccgaag cgattcagag ttgtaaagtg cgtctgaccc cctgattata
     1201 tctcaaagtc ccttcacaaa ttcagacatg atgcgataaa gtgccggatt gtcattcgcg
     1261 tcttgtgact gtatagaaag tgccggcatc ctcttttctt ttcctctttg gccgagcgac
     1321 tcagagttgt gaagcctgct ggtatctgtc tgttcccttg ccctgataaa gaatatcgaa
     1381 gagcatttct ttgattcaca atacgttgac gaatggtgtg atggatccta agcagcgcca
     1441 gccctttgaa cttgtgtcga aaaaaaaaaa aaaacagcat atgatacgta aagggaagaa
     1501 aaggatggag ctgactgttt tgacaggcat aattttgagc gtgcttcatt ggccggttta
     1561 tctgtattaa cacggaagag atgtagaaac tagcttcctg gtttcagaga caaggagcca
     1621 tgaagatcct gaggattcaa cggcgttgct gagggtggga aggatggtat tgttgtctgt
     1681 agatagatta atgggcatat aatgtcaaaa aagatgattg acacgatgtc tgtctcgaat
     1741 tcgcagtgtg ctaaaattgt atcacttctc taatatgtat atatacaccc tctaaggaaa
     1801 attaacccct taccgccttc actcagaaga actcgtcaag aaggcgatag aaggcgatgc
     1861 gctgcgaatc gggagcggcg ataccgtaaa gcacgaggaa gcggtcagcc cattcgccgc
     1921 caagctcttc agcaatatca cgggtagcca acgctatgtc ctgatagcgg tccgccacac
     1981 ccagccggcc acagtcgatg aatccagaaa agcggccatt ttccaccatg atattcggca
     2041 agcaggcatc gccatgggtc acgacgagat cctcgccgtc gggcatgcgc gccttgagcc
     2101 tggcgaacag ttcggctggc gcgagcccct gatgctcttc gtccagatca tcctgatcga
     2161 caagaccggc ttccatccga gtacgtgctc gctcgatgcg atgtttcgct tggtggtcga
     2221 atgggcaggt agccggatca agcgtatgca gccgccgcat tgcatcagcc atgatggata
     2281 ctttctcggc aggagcaagg tgggatgaca ggagatcctg ccccggcact tcgcccaata
     2341 gcagccagtc ccttcccgct tcagtgacaa cgtcgagcac agctgcgcaa ggaacgcccg
     2401 tcgtggccag ccacgatagc cgcgctgcct cgtcctgcag ttcattcagg gcaccggaca
     2461 ggtcggtctt gacaaaaaga accgggcgcc cctgcgctga cagccggaac acggcggcat
     2521 cagagcagcc gattgtctgt tgtgcccagt catagccgaa tagcctctcc acccaagcgg
     2581 ccggagaacc tgcgtgcaat ccatcttgtt caatcataga catgttgggc gagtttacta
     2641 atggaaaaag acaattagca aagaggctgc atattaggag acgcggtgaa gattggggag
     2701 gacaataggg tccgtgcacc aaaaagtcct gacggcagct ctgttcaacg cacatccatc
     2761 tcttccagct atgcgtgtgg caccagcacg gatggattgt tgcaccaaaa ggacaaggcg
     2821 agatggatgc aggtcgaggc gatgcggtgc tatcggccct cgttgtcgaa cgccacgtcg
     2881 tcctctcggc gaccgtcaat cctggaagga aatgaccaag cgtgcaccca gcatccagca
     2941 tccaacatcc agcacccagc acctagcacc catgatccaa tggccggcat gcagatcagc
     3001 gtggggagag caccatctag cagcccctgg caggcccagc attgtgctcg gatgtgcgat
     3061 aggcggtggg tgcgataagg tggattagtg gaggatggag gactgagtgg cgggcaaggg
     3121 aagggggcgg gaagatgact caccttgggc agtgggtggg ggatgcggta taggggatgt
     3181 atgtggatgc tggcggagga tagaagctgt aagctgagct ggtgccaacg gtgacgctgt
     3241 gagagtggtc tcggcgaccc tgacggcgac tcggcgttac cccgtgtggc acaccggacg
     3301 cgcgtcgagt gacgttgctg ctattgttgt taccatcatc ctctcctccg acaaccatac
     3361 actcagtgca caccatgcca ctcgaatcct gcatgcttat gtgagtcctc cccttcccgt
     3421 gcgccgctct ccagctcaca tcctcgcagc catggactca accctatctc ggtctattct
     3481 tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaaatg agctgattta
     3541 acaaaaattt aacgcgaatt ttaacaaaat attaacgctt acaatttcca ttcgccattc
     3601 aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg
     3661 gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca
     3721 cgacgttgta aaacgacggc cagtgagcgc gcgtaatacg actcactata gggcgaattg
     3781 ggtaccgggc cccccctcga tagtgaagtc gtattgtctc tgcaatgtaa ttctctttgc
     3841 catgcatatg cttgagcctg tgccttctct ttaatcgaca gcactgacaa tctcttgcag
     3901 tttcgtcgca gtagtcacgc gtatatgttt ccatagttcc ggcgggaagt tatcttacta
     3961 aatacggtat cgaaggaatt acacaatatt tgtactccgg cggtggaaac aggtcttgtc
     4021 gaaccctgat gaagcgaaag ctaagaggtt cgaaagtagc aaaatcatac tttaaaatct
     4081 tttatacgcg agtccaactt cgctgacaag aaaatggtag tgggccttgc gaatgaatga
     4141 ggagagaaag ttttagctgt ggattcccag caggcctcct tacttaagtt tgattaaaaa
     4201 ttattacgaa aagatatata tagtaataaa tttgaaatga actaatcata atcgaaaaat
     4261 tcccgttcaa cataaattcc cgttcattta aattcccgtt caacataaat tcccgttcat
     4321 ttaaattccc gttcaacata aaattcccgt tcataaattc ccatggcatt catcatcatg
     4381 aatcctttgt tgtatagtta ttatataacg gatattcccg ttcgtatcca ttgcatatct
     4441 tatcgcatag tcttcccgtt catcgtctca tcatcaagtt attgattgtc aattcacgct
     4501 tacatattcc cgttcttctg tctaccatct atctatcaca tcatctgtga attcttcccg
     4561 tttttatggg aagactgtcg tcatgttgtc aattgagcag cagacataat cgatgctata
     4621 tatctagagc ttcacggctc aggctgaact tggattaatg tggactactt tcacaacgaa
     4681 tacttatcag cactcaattc tctcctgaga atggtctcca agggcgagga ggacaacatg
     4741 gcctctctcc ctgccactca cgagttgcac atcttcggct ccatcaacgg tgtcgacttc
     4801 gacatggtcg gtcagggcac cggcaaccct aacgacggtt acgaggagtt gaaccttaag
     4861 tccaccaagg gtgacctcca gttctccccc tggattcttg tccctcacat cggctacggc
     4921 ttccaccagt accttcccta ccctgacggc atgtcccctt tccaggccgc catggttgac
     4981 ggctccggtt accaggtcca ccgtactatg cagttcgagg acggtgcctc ccttactgtt
     5041 aactaccgtt acacctacga gggttcccac atcaagggtg aggcccaggt caagggcact
     5101 ggtttccctg ctgacggtcc tgtcatgacc aactccctta ccgctgccga ctggtgcagg
     5161 tccaagaaga cttaccccaa cgacaagacc atcatctcca ccttcaagtg gtcctacacc
     5221 actggtaacg gcaagcgtta ccgatccact gcccgaacca cctacacctt cgccaagcct
     5281 atggccgcta actaccttaa gaaccagccc atgtacgttt tccgtaagac cgagctcaag
     5341 cactccaaga ccgagctcaa cttcaaggag tggcagaagg ccttcaccga cgtcatgggc
     5401 atggacgagc tttacaaggg ttctggcggt ggtggctccg gcatgcctca gtcctgggag
     5461 gagctcgccg ctgacaagcg tgcccgtctc gctaagacca tccctgacga gtggaaggtc
     5521 cagaccctcc ctgccgagga ctccgttatt gacttcccta agaagtctgg catcctttct
     5581 gaggccgagc tcaagatcac tgaggcctcc gctgctgacc ttgtctccaa gctcgccgcc
     5641 ggtgagttga cctctgtcga ggttaccctt gctttctgta agcgagctgc tatcgcccag
     5701 cagttgacta actgcgccca cgagttcttc cctgacgccg ctctcgccca ggctagggag
     5761 ctcgacgagt actacgctaa gcacaagagg cccgttggtc ctctccacgg cctccccatc
     5821 tctctcaagg accagcttcg agtcaagggc tacgagactt ctatgggcta catctcttgg
     5881 cttaacaagt acgacgaggg cgactctgtt ctcactacca tgctccgtaa ggccggtgcc
     5941 gtcttctacg tcaagacctc tgtcccccag accctcatgg tctgcgagac tgtcaacaac
     6001 atcatcggcc gtaccgtcaa ccctcgtaac aagaactggt cttgcggcgg ctcctctggt
     6061 ggtgagggtg ccatcgttgg cattcgtggt ggcgtcatcg gtgttggtac cgacattggt
     6121 ggctctattc gagtccccgc cgccttcaac ttcctctacg gtcttaggcc ctcccacggc
     6181 cgactcccct acgctaagat ggccaactcc atggagggtc aggagaccgt ccactccgtt
     6241 gtcggcccca ttacccactc tgttgaggac ctccgtctct tcaccaagtc cgtcctcggt
     6301 caggagcctt ggaagtacga ctccaaggtc atccccatgc cctggcgtca gtccgagtct
     6361 gacattattg cctccaagat caagaacggc ggcctcaaca tcggctacta caacttcgac
     6421 ggcaacgtcc ttcctcaccc tcctatcctc cgtggcgtcg agaccaccgt cgccgctctc
     6481 gccaaggccg gtcacaccgt caccccctgg accccttaca agcacgactt cggccacgac
     6541 ctcatctccc acatctacgc cgctgacggc tccgccgacg ttatgcgtga catctccgct
     6601 tccggcgagc ccgccattcc taacatcaag gaccttctca accccaacat caaggctgtt
     6661 aacatgaacg agctctggga cacccacctc cagaagtgga actaccagat ggagtacctt
     6721 gagaagtggc gagaggctga ggagaaggcc ggcaaggagg tgagtagatc acaatttgta
     6781 acaggttgga tgataagtcc ccggtctaac atgtagcgac gacactgaag ctcacttctt
     6841 gtagctcgac gccatcatcg cccccattac ccctaccgct gccgttcgac acgaccagtt
     6901 ccgatactac ggctacgcct ctgtcatcaa cctcctcgac ttcacctccg tcgttgttcc
     6961 cgttaccttc gccgacaaga acatcgacaa gaagaacgag tccttcaagg ccgtttccga
     7021 gcttgacgcc ctcgtccagg aggagtacga ccccgaggcc taccacggcg ctcccgttgc
     7081 tgtccaggtt atcggtcgaa ggctctccga ggagaggacc ttggccattg ctgaggaggt
     7141 cggcaagttg ctcggtaacg tcgtcactcc ttagaaggtt gagcgcgtaa tagtgggttg
     7201 gtcttgtctc tatccattca tctgtgagac atactgattt caactttttc atcagataat
     7261 aaggatggtg tgagataagc aaggctgggt tatgaggttt gggactagat ggtgtttcgc
     7321 gtctaaaggt gagtcttcgt tggttcaatc acattgatct gatcgctgat caattgtcag
     7381 cctctgaatg tgctcttgaa gaaaatggtt gtctgtaaga atccagaacc aaaaacggtc
     7441 atttcaagta tgtatcctcc gtgaagttcc tttagctctt gctaatattg gactttagcg
     7501 tgctttagcc aaagacaggg tcgcgaggtt aagaggatgg gccaggaaaa atctaagtta
     7561 gatttgtcac acaaagaaaa agattgtctc agttttggtt ttgtttggtt tgatttatgc
     7621 ataggaaata tttatgatcg aaatcactca tttcctgctt cgtaaatatc atctactata
     7681 tgcttcgtaa atgtccatct cctacatatt gtggatatga taaatgcaat cctctcgtct
     7741 acactctcgg cttatgcctt tgtcttcttt gcgctccttt tagccttctt tttattccta
     7801 gtcttcaagc ccatcggtgg agctccagct tttgttccct ttagtgaggg ttaattgcgc
     7861 gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc
     7921 cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct
     7981 aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc
     8041 agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt
     8101 ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag
     8161 ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca
     8221 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt
     8281 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc
     8341 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct
     8401 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg
     8461 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca
     8521 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact
     8581 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta
     8641 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta
     8701 actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct
     8761 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt
     8821 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga
     8881 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca
     8941 tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat
     9001 caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg
     9061 cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt
     9121 agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag
     9181 acccacgctc accggctcca gatttatcag caataaacca gccagccgga agggccgagc
     9241 gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag
     9301 ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca
     9361 tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa
     9421 ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga
     9481 tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata
     9541 attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca
     9601 agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg
     9661 ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg
     9721 ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg
     9781 cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag
     9841 gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac
     9901 tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca
     9961 tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag
    10021 tgccacc
//
