LOCUS       sequence-443456-       10339 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  TEF1(p)-amdS:mNeonGreen fusion reporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10339)
  AUTHORS   Phillips-Rose LS, Yu CK, West NP, Fraser JA
  TITLE     A Chimeric ORF Fusion Phenotypic Reporter for Cryptococcus 
            neoformans.
  JOURNAL   J Fungi (Basel). 2024 Aug 12;10(8):567. doi: 10.3390/jof10080567.
  PUBMED    39194893
REFERENCE   2  (bases 1 to 10339)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Fungi 
            (Basel)."; date: "2024-08-12"; volume: "10(8):567. doi"; pages: " 
            10.3390/jof10080567"
COMMENT     Sequence Label: pLSPR29
FEATURES             Location/Qualifiers
     source          1..10339
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(1824..2624)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MSMIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQG
                     RPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL
                     LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEE
                     HQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQD
                     IALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(1935..1954)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     2545..2564
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     3714..3736
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3728..3745
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3729..3745
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3755..3774
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        3755..3773
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      7032..7041
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(8151..8171)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(8151..8169)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(8190..8206)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8190..8206)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8203..8225)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8214..8230
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8238..8244,8245..8262,8263..8268))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8283..8304
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8421..8438)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8592..9180)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8672..8691)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9351..10142,10143..10211))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9974..9993
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10212..10316)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctgacgcgcc ctgtagcggc aagatctgcc catagacttc aaatcatgga attggacgtt
       61 tatcttcttg ctttggatat ccaccctatc gtacaatctt ggcactttgg atcatacgag
      121 gcactttgcg gactagggaa tggttatcat caatttcttg gacctctcca tgacacctgg
      181 catcgtcgag ctaccacagc cccctgaatt cttaaaggcg aacccccctt cctaggaacc
      241 ttgagcgctg gactggatgg cactctgaga ccgcctatgc ttaaggacgc gagcttaccc
      301 ttccattgat ggaagtcgat catttatgat aatagaacac ggcggaactt gactttacag
      361 gccaagggaa gcaacaggag aagaagctaa ggttataaca ggctcagcat atttgtatat
      421 acggtgccat tcaacgctga acactttgta ctcacggtct gcccgttgaa taacaccatc
      481 tgctgatgat catcccaatt tgtgagtctt atttgccatt gatcctggtg tataaccgat
      541 actgaagccc tttaggtctg atcagttata gacgtggtat attcattctt cctagattag
      601 ctatgccgaa agagatggat accttgatgt gactggaatg ccatgaagca ggagattgta
      661 gcagctcaaa ttggagaagg atgttgcaac cgtcggccgt gaaggtcttg tcatatcttt
      721 gtctgcctgg gagaatatgt acaagtgccc cgatgttact gatggaaaac atcgtgtggc
      781 gcgccacgat acttgtgtta attaaaccaa accagtctgt gccaagtgga ttgcttatgc
      841 atcgagtggc gagcccactg acctctgggc cccttttttg aactataaaa agcggccaag
      901 tctgatttcg gtctcacaat catcaacatc gacttctctt ttcgcctcga tcaacctaaa
      961 caacggtcac tcagccatga actggacaat ggaatatttc gccttgcctt ggatctttgg
     1021 cctctagact caatcaacgt caggataaag gtgagcatag gttgctcact cactgtagcc
     1081 tttttttccc ttcttcttgg tccagatggt tcccaaggct ctataaagtg tatctgtcag
     1141 ttccacttcc ttactccgaa gcgattcaga gttgtaaagt gcgtctgacc ccctgattat
     1201 atctcaaagt cccttcacaa attcagacat gatgcgataa agtgccggat tgtcattcgc
     1261 gtcttgtgac tgtatagaaa gtgccggcat cctcttttct tttcctcttt ggccgagcga
     1321 ctcagagttg tgaagcctgc tggtatctgt ctgttccctt gccctgataa agaatatcga
     1381 agagcatttc tttgattcac aatacgttga cgaatggtgt gatggatcct aagcagcgcc
     1441 agccctttga acttgtgtcg aaaaaaaaaa aaaaacagca tatgatacgt aaagggaaga
     1501 aaaggatgga gctgactgtt ttgacaggca taattttgag cgtgcttcat tggccggttt
     1561 atctgtatta acacggaaga gatgtagaaa ctagcttcct ggtttcagag acaaggagcc
     1621 atgaagatcc tgaggattca acggcgttgc tgagggtggg aaggatggta ttgttgtctg
     1681 tagatagatt aatgggcata taatgtcaaa aaagatgatt gacacgatgt ctgtctcgaa
     1741 ttcgcagtgt gctaaaattg tatcacttct ctaatatgta tatatacacc ctctaaggaa
     1801 aattaacccc ttaccgcctt cactcagaag aactcgtcaa gaaggcgata gaaggcgatg
     1861 cgctgcgaat cgggagcggc gataccgtaa agcacgagga agcggtcagc ccattcgccg
     1921 ccaagctctt cagcaatatc acgggtagcc aacgctatgt cctgatagcg gtccgccaca
     1981 cccagccggc cacagtcgat gaatccagaa aagcggccat tttccaccat gatattcggc
     2041 aagcaggcat cgccatgggt cacgacgaga tcctcgccgt cgggcatgcg cgccttgagc
     2101 ctggcgaaca gttcggctgg cgcgagcccc tgatgctctt cgtccagatc atcctgatcg
     2161 acaagaccgg cttccatccg agtacgtgct cgctcgatgc gatgtttcgc ttggtggtcg
     2221 aatgggcagg tagccggatc aagcgtatgc agccgccgca ttgcatcagc catgatggat
     2281 actttctcgg caggagcaag gtgggatgac aggagatcct gccccggcac ttcgcccaat
     2341 agcagccagt cccttcccgc ttcagtgaca acgtcgagca cagctgcgca aggaacgccc
     2401 gtcgtggcca gccacgatag ccgcgctgcc tcgtcctgca gttcattcag ggcaccggac
     2461 aggtcggtct tgacaaaaag aaccgggcgc ccctgcgctg acagccggaa cacggcggca
     2521 tcagagcagc cgattgtctg ttgtgcccag tcatagccga atagcctctc cacccaagcg
     2581 gccggagaac ctgcgtgcaa tccatcttgt tcaatcatag acatgttggg cgagtttact
     2641 aatggaaaaa gacaattagc aaagaggctg catattagga gacgcggtga agattgggga
     2701 ggacaatagg gtccgtgcac caaaaagtcc tgacggcagc tctgttcaac gcacatccat
     2761 ctcttccagc tatgcgtgtg gcaccagcac ggatggattg ttgcaccaaa aggacaaggc
     2821 gagatggatg caggtcgagg cgatgcggtg ctatcggccc tcgttgtcga acgccacgtc
     2881 gtcctctcgg cgaccgtcaa tcctggaagg aaatgaccaa gcgtgcaccc agcatccagc
     2941 atccaacatc cagcacccag cacctagcac ccatgatcca atggccggca tgcagatcag
     3001 cgtggggaga gcaccatcta gcagcccctg gcaggcccag cattgtgctc ggatgtgcga
     3061 taggcggtgg gtgcgataag gtggattagt ggaggatgga ggactgagtg gcgggcaagg
     3121 gaagggggcg ggaagatgac tcaccttggg cagtgggtgg gggatgcggt ataggggatg
     3181 tatgtggatg ctggcggagg atagaagctg taagctgagc tggtgccaac ggtgacgctg
     3241 tgagagtggt ctcggcgacc ctgacggcga ctcggcgtta ccccgtgtgg cacaccggac
     3301 gcgcgtcgag tgacgttgct gctattgttg ttaccatcat cctctcctcc gacaaccata
     3361 cactcagtgc acaccatgcc actcgaatcc tgcatgctta tgtgagtcct ccccttcccg
     3421 tgcgccgctc tccagctcac atcctcgcag ccatggactc aaccctatct cggtctattc
     3481 ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaaat gagctgattt
     3541 aacaaaaatt taacgcgaat tttaacaaaa tattaacgct tacaatttcc attcgccatt
     3601 caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat tacgccagct
     3661 ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt tttcccagtc
     3721 acgacgttgt aaaacgacgg ccagtgagcg cgcgtaatac gactcactat agggcgaatt
     3781 gggtaccggg ccccccctcg acattctatg ctatacggta caagattgtg gctactatgc
     3841 aagctccata gcgaatgatc ttacactgac cttgtcgacc gttctgaatg attaacaatg
     3901 gtcgcaagtg tggttgaccc acctgcgggc gcttcttaac tatctatcat cggtatgtca
     3961 agtatcactc cctttaaaca aaagaataag agcataatac agcagtctca tcatggcaac
     4021 ccttcgcgca atgtacaaaa agcagccaga agcccccacg cacaagcaat aatgtcgtgt
     4081 tctaccctgc catgaacaag cgtcatggcc attagcgaac gccccaatat tcagccccat
     4141 tcgccaagca agttgcctcg cccccttccc agaagatcat gggcatctcg tcttcttcta
     4201 atttcgcatt ttgtgaagct ccattcatgc acgtcggctt ctggacgact cttatcatat
     4261 gcattggcct gctcggtgtg aggatgagcc actcccacat gtttagacgg caggggcctc
     4321 ttgccacccg ttgaattttt ggtgcgaaga cgtgcctggg aacgacctcc actttaaatt
     4381 tctccgttgt tgcggtcgct ccatcctcct cacatttctt gcctcctaag tcccatgtgc
     4441 cacactcggt gaaaaacgga gtttgatacg gatgaaattt ccagtgagct tttcacattc
     4501 tggaggtttc gaaaaaaatt gtgggatttt ctgaattaat ccgagaatcc gccctgccca
     4561 tcaaccggaa agaattaaat ccctcgcaca ttcctttctc ccaccacctt ctttatctct
     4621 acattcttta accaaaacac ctaggtgagt acccaaagtc ttttgacacc tttgaaaatc
     4681 cgcgccagtc gtagggcacg taaaacaatt ctcgctggtg gcttctgcag gtgaagtcgg
     4741 ggcagagtgg agctgagcat ggtcgaccac ccacatcatt ctgtcttgtt tttggcgatg
     4801 tcttccatgc ttttgccctc atctgttgcg tcgactgcag agtactgttt tgccactact
     4861 gagctcaaca tgggcttgtg tgtggtgaag aaaaaaattt ggtggctctt gaaaattttc
     4921 aacccatcgg atacatgccc ttccaactca caacgcccta acactttatt gtcctatcat
     4981 tgcctcttcg cacatgctaa cgatctccac agaaaacttc aaaatgcctc agtcctggga
     5041 ggagctcgcc gctgacaagc gtgcccgtct cgctaagacc atccctgacg agtggaaggt
     5101 ccagaccctc cctgccgagg actccgttat tgacttccct aagaagtctg gcatcctttc
     5161 tgaggccgag ctcaagatca ctgaggcctc cgctgctgac cttgtctcca agctcgccgc
     5221 cggtgagttg acctctgtcg aggttaccct tgctttctgt aagcgagctg ctatcgccca
     5281 gcagttgact aactgcgccc acgagttctt ccctgacgcc gctctcgccc aggctaggga
     5341 gctcgacgag tactacgcta agcacaagag gcccgttggt cctctccacg gcctccccat
     5401 ctctctcaag gaccagcttc gagtcaaggg ctacgagact tctatgggct acatctcttg
     5461 gcttaacaag tacgacgagg gcgactctgt tctcactacc atgctccgta aggccggtgc
     5521 cgtcttctac gtcaagacct ctgtccccca gaccctcatg gtctgcgaga ctgtcaacaa
     5581 catcatcggc cgtaccgtca accctcgtaa caagaactgg tcttgcggcg gctcctctgg
     5641 tggtgagggt gccatcgttg gcattcgtgg tggcgtcatc ggtgttggta ccgacattgg
     5701 tggctctatt cgagtccccg ccgccttcaa cttcctctac ggtcttaggc cctcccacgg
     5761 ccgactcccc tacgctaaga tggccaactc catggagggt caggagaccg tccactccgt
     5821 tgtcggcccc attacccact ctgttgagga cctccgtctc ttcaccaagt ccgtcctcgg
     5881 tcaggagcct tggaagtacg actccaaggt catccccatg ccctggcgtc agtccgagtc
     5941 tgacattatt gcctccaaga tcaagaacgg cggcctcaac atcggctact acaacttcga
     6001 cggcaacgtc cttcctcacc ctcctatcct ccgtggcgtc gagaccaccg tcgccgctct
     6061 cgccaaggcc ggtcacaccg tcaccccctg gaccccttac aagcacgact tcggccacga
     6121 cctcatctcc cacatctacg ccgctgacgg ctccgccgac gttatgcgtg acatctccgc
     6181 ttccggcgag cccgccattc ctaacatcaa ggaccttctc aaccccaaca tcaaggctgt
     6241 taacatgaac gagctctggg acacccacct ccagaagtgg aactaccaga tggagtacct
     6301 tgagaagtgg cgagaggctg aggagaaggc cggcaaggag gtgagtagat cacaatttgt
     6361 aacaggttgg atgataagtc cccggtctaa catgtagcga cgacactgaa gctcacttct
     6421 tgtagctcga cgccatcatc gcccccatta cccctaccgc tgccgttcga cacgaccagt
     6481 tccgatacta cggctacgcc tctgtcatca acctcctcga cttcacctcc gtcgttgttc
     6541 ccgttacctt cgccgacaag aacatcgaca agaagaacga gtccttcaag gccgtttccg
     6601 agcttgacgc cctcgtccag gaggagtacg accccgaggc ctaccacggc gctcccgttg
     6661 ctgtccaggt tatcggtcga aggctctccg aggagaggac cttggccatt gctgaggagg
     6721 tcggcaagtt gctcggtaac gtcgtcactc ctggttctgg cggtggtggc tccggcatgg
     6781 tctccaaggg cgaggaggac aacatggcct ctctccctgc cactcacgag ttgcacatct
     6841 tcggctccat caacggtgtc gacttcgaca tggtcggtca gggcaccggc aaccctaacg
     6901 acggttacga ggagttgaac cttaagtcca ccaagggtga cctccagttc tccccctgga
     6961 ttcttgtccc tcacatcggc tacggcttcc accagtacct tccctaccct gacggcatgt
     7021 cccctttcca ggccgccatg gttgacggct ccggttacca ggtccaccgt actatgcagt
     7081 tcgaggacgg tgcctccctt actgttaact accgttacac ctacgagggt tcccacatca
     7141 agggtgaggc ccaggtcaag ggcactggtt tccctgctga cggtcctgtc atgaccaact
     7201 cccttaccgc tgccgactgg tgcaggtcca agaagactta ccccaacgac aagaccatca
     7261 tctccacctt caagtggtcc tacaccactg gtaacggcaa gcgttaccga tccactgccc
     7321 gaaccaccta caccttcgcc aagcctatgg ccgctaacta ccttaagaac cagcccatgt
     7381 acgttttccg taagaccgag ctcaagcact ccaagaccga gctcaacttc aaggagtggc
     7441 agaaggcctt caccgacgtc atgggcatgg acgagcttta caagtaaaag gttgagcgcg
     7501 taatagtggg ttggtcttgt ctctatccat tcatctgtga gacatactga tttcaacttt
     7561 ttcatcagat aataaggatg gtgtgagata agcaaggctg ggttatgagg tttgggacta
     7621 gatggtgttt cgcgtctaaa ggtgagtctt cgttggttca atcacattga tctgatcgct
     7681 gatcaattgt cagcctctga atgtgctctt gaagaaaatg gttgtctgta agaatccaga
     7741 accaaaaacg gtcatttcaa gtatgtatcc tccgtgaagt tcctttagct cttgctaata
     7801 ttggacttta gcgtgcttta gccaaagaca gggtcgcgag gttaagagga tgggccagga
     7861 aaaatctaag ttagatttgt cacacaaaga aaaagattgt ctcagttttg gttttgtttg
     7921 gtttgattta tgcataggaa atatttatga tcgaaatcac tcatttcctg cttcgtaaat
     7981 atcatctact atatgcttcg taaatgtcca tctcctacat attgtggata tgataaatgc
     8041 aatcctctcg tctacactct cggcttatgc ctttgtcttc tttgcgctcc ttttagcctt
     8101 ctttttattc ctagtcttca agcccatcgg tggagctcca gcttttgttc cctttagtga
     8161 gggttaattg cgcgcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat
     8221 ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc
     8281 taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga
     8341 aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt
     8401 attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg
     8461 cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac
     8521 gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg
     8581 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca
     8641 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc
     8701 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc
     8761 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag
     8821 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc
     8881 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca
     8941 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg
     9001 aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg
     9061 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct
     9121 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa
     9181 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
     9241 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
     9301 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
     9361 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
     9421 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
     9481 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
     9541 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
     9601 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
     9661 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
     9721 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
     9781 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
     9841 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
     9901 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
     9961 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
    10021 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
    10081 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
    10141 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
    10201 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
    10261 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
    10321 tttccccgaa aagtgccac
//
