LOCUS       sequence-443458-        6789 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Donor plasmid of hAT-6_DR Transposon.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6789)
  AUTHORS   Zhang T, Tan S, Tang N, Li Y, Zhang C, Sun J, Guo Y, Gao H, Cai Y, 
            Sun W, Wang C, Fu L, Ma H, Wu Y, Hu X, Zhang X, Gee P, Yan W, Zhao 
            Y, Chen Q, Guo B, Wang H, Zhang YE
  TITLE     Heterologous survey of 130 DNA transposons in human cells highlights
            their functional divergence and expands the genome engineering 
            toolbox.
  JOURNAL   Cell. 2024 Jul 11;187(14):3741-3760.e30. doi: 
            10.1016/j.cell.2024.05.007. Epub 2024 Jun 5.
  PUBMED    38843831
REFERENCE   2  (bases 1 to 6789)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2024.05.007"; journalName: "Cell"; date: 
            "2024-07-11- 11"; volume: "187"; issue: "14"; pages: "3741-3760.e30"
COMMENT     Sequence Label: pMV-hAT-6_DR
FEATURES             Location/Qualifiers
     source          1..6789
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     93..111
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     120..137
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     121..137
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        440..939
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(475..498)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     872..891
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     regulatory      953..962
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             959..1558
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MGTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIE
                     RVTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRL
                     AAQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLET
                     SAPRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     1458..1478
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     CDS             1565..1621
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             join(1622..1624,1625..1627,1628..2341)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(1622..1624,1625..1627,1628..2338)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1667..1688)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1928..1947)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2275..2296
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(2363..2380)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    2369..2593
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(4891..4907)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4891..4907)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4919..4936)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5090..5678)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5170..5189)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5849..6640,6641..6709))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLRNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERKRQIAEIGAS
                     LIKHW"
     primer_bind     6472..6491
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(join(6710..6789,1..25))
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtctaag
       61 aaaccattat tatcatgaca ttaacctata aaaataggcg tatcacgagg ccctttcgtt
      121 gtaaaacgac ggccagtcga accacgcaat gcgtctcgat ccgcagtgtc ttgcgtctct
      181 aagcttctat gtattaggct tgggcgatat tacggtaata tcgtataccg cgggatctaa
      241 aaatagcaac ggtgtcagtt tcaataccgt tataccgtca taaaaaaatt tttttaaaag
      301 gcacttttat aggagtcaag gattaaatat tatgaaaata taatttattt aataattaaa
      361 atgcgtatgt gtcgttgtca ttacgggaca gcgtggagaa agagagaggt gaggaaacac
      421 cggtgctagc aattctaccg ggtaggggag gcgcttttcc caaggcagtc tggagcatgc
      481 gctttagcag ccccgctggg cacttggcgc tacacaagtg gcctctggcc tcgcacacat
      541 tccacatcca ccggtaggcg ccaaccggct ccgttctttg gtggcccctt cgcgccacct
      601 tctactcctc ccctagtcag gaagttcccc cccgccccgc agctcgcgtc gtgcaggacg
      661 tgacaaatgg aagtagcacg tctcactagt ctcgtgcaga tggacagcac cgctgagcaa
      721 tggaagcggg taggcctttg gggcagcggc caatagcagc tttgctcctt cgctttctgg
      781 gctcagaggc tgggaagggg tgggtccggg ggcgggctca ggggcgggct caggggcggg
      841 gcgggcgccc gaaggtcctc cggaggcccg gcattctgca cgcttcaaaa gcgcacgtct
      901 gccgcgctgt tctcctcttc ctcatctccg ggcctttcga cctgcagcca acgccaccat
      961 ggggaccgag tacaagccca cggtgcgcct cgccacccgc gacgacgtcc cccgggccgt
     1021 acgcaccctc gccgccgcgt tcgccgacta ccccgccacg cgccacaccg tcgacccgga
     1081 ccgccacatc gagcgggtca ccgagctgca agaactcttc ctcacgcgcg tcgggctcga
     1141 catcggcaag gtgtgggtcg cggacgacgg cgccgcggtg gcggtctgga ccacgccgga
     1201 gagcgtcgaa gcgggggcgg tgttcgccga gatcggcccg cgcatggccg agttgagcgg
     1261 ttcccggctg gccgcgcagc aacagatgga aggcctcctg gcgccgcacc ggcccaagga
     1321 gcccgcgtgg ttcctggcca ccgtcggcgt ctcgcccgac caccagggca agggtctggg
     1381 cagcgccgtc gtgctccccg gagtggaggc ggccgagcgc gccggggtgc ccgccttcct
     1441 ggagacctcc gcgccccgca acctcccctt ctacgagcgg ctcggcttca ccgtcaccgc
     1501 cgacgtcgag gtgcccgaag gaccgcgcac ctggtgcatg acccgcaagc ccggtgccat
     1561 cgatgccact aacttctccc tgttgaaaca agcaggggat gtcgaagaga atcccgggcc
     1621 aatggtgagc aagggcgagg agctgttcac cggggtggtg cccatcctgg tcgagctgga
     1681 cggcgacgta aacggccaca agttcagcgt gtccggcgag ggcgagggcg atgccaccta
     1741 cggcaagctg accctgaagt tcatctgcac caccggcaag ctgcccgtgc cctggcccac
     1801 cctcgtgacc accctgacct acggcgtgca gtgcttcagc cgctaccccg accacatgaa
     1861 gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc gcaccatctt
     1921 cttcaaggac gacggcaact acaagacccg cgccgaggtg aagttcgagg gcgacaccct
     1981 ggtgaaccgc atcgagctga agggcatcga cttcaaggag gacggcaaca tcctggggca
     2041 caagctggag tacaactaca acagccacaa cgtctatatc atggccgaca agcagaagaa
     2101 cggcatcaag gtgaacttca agatccgcca caacatcgag gacggcagcg tgcagctcgc
     2161 cgaccactac cagcagaaca cccccatcgg cgacggcccc gtgctgctgc ccgacaacca
     2221 ctacctgagc acccagtccg ccctgagcaa agaccccaac gagaagcgcg atcacatggt
     2281 cctgctggag ttcgtgaccg ccgccgggat cactctcggc atggacgagc tgtacaagta
     2341 attaattaag ctcgctgatc agcctcgact gtgccttcta gttgccagcc atctgttgtt
     2401 tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt cctttcctaa
     2461 taaaatgagg aaattgcatc gcattgtctg agtaggtgtc attctattct ggggggtggg
     2521 gtggggcagg acagcaaggg ggaggattgg gaagacaata gcaggcatgc tggggatgcg
     2581 gtgggctcta tggcttctga ggcgcgccct cgagaataaa ataaattatt aaataaataa
     2641 aataaaaaaa catgttttta attgttttta tttctgggac ttatttagcc tgagaaatgt
     2701 gcatgcaatt ttaactaaat agaataatgt acattaatgg acgtatttat ttattcgtgt
     2761 atttaggcct atttattcat ttatttatcc gttaaactaa taaataggcc ttaataagta
     2821 aatttcgttt attttaggcc tatttattca ttcattcgtt tatgtaggcc tattcattca
     2881 ttcgttcgtt tattaaggcc tatttattcg tttatttttt tatttaagcc tatttattct
     2941 tttatttaag cctattcatt tgtttattta agcctattta ttcgtttatt taagctcatt
     3001 tattaattta ttcgtttatt taagcctatc tattcgttta tttaagccta tttattcgtt
     3061 tatttaagcc tatttattaa tttatttgtt tatttaagct tatttattaa gttattcgtt
     3121 catttaagct tatttattca ttcatacgtt tatttaagcc tatttattcg tttatttaag
     3181 cctatttatt catttatcta agcctattta ttgtttattt aggctaattc atttattttg
     3241 ttaaacggat aagtggaaaa atacgccttt gtttatttag acttactcat taattatttc
     3301 gtttatttat gccaatttat tcattcgttt atttaggcca attcattaat ttcatcgttt
     3361 attcattcat taattcatcc atccatccgt cggcaggact gcgcaactcg ctggggctcc
     3421 aaactggcaa tggtggagcg catcctcgag caggcccaag cgatcagaca cgtcctgtct
     3481 gatgacagaa ggagcagcct gtccctgacc tggcaggaca tggacgtctt gaaagctatt
     3541 cacgaagcac tgaaaccagt cggtgacttc actgatattt tgtcaggaga aaattatgtg
     3601 accagttcct gtatcctccc catactacag ctctgcaggg acaatgtttt ggctgcgtca
     3661 gaaaatgacc ttcagctaac aaagtcaatc aaaactggaa ttctaacaaa gctggaggcc
     3721 aagtatgaat ccagttcagt gcgcaaaata ttgcgaaaat gcactttcct cgacccgcgt
     3781 taccgcggag gatatgaggc agatgacaac gcattggctg agacaaaggc tgaattacag
     3841 gctgagatcg tcagcttaga ggcagcaggt ccagtggcca taagagtgga agagggagag
     3901 gcgcaactag aacccttacg aaaaaaaatg actctgggaa gtatgctgca aaaaaaacct
     3961 gatgcaatgg caggtgccgt cggcagcggc accgtagaag accgagtcgg agctgaaata
     4021 acagcttact gtttggagcc agttactcaa ggagacgagg acccacttct ttggtggaaa
     4081 tctgctgccg ggcgttttcc ccagatgtca cgagtggcac gaaaatacct gtgcgtctgt
     4141 gccacaagca caccatcgga acgggttttc agcaccgcag gtaaagttgt cggtccacaa
     4201 cgttccctgt taaagcccga caaagtaaac atgctggttt ttctagcgaa aaatattgat
     4261 tagattgatg ccaattgcaa tttgtttcta ttcgcattgt ttaattttgt ttaattaatt
     4321 caggtatttt tatttgcttt acgttaggaa ttatattaga agactggaaa taagtttttg
     4381 tttaggacca ttgctctgca atacttttta taataaggat gatgtttatg ttagcctaat
     4441 taatctctgg ttgactgttg tgtttttttg catattttat aattttataa ctgctcttct
     4501 ttgttattaa aagttctggc ggtgtgattt aattgatttg tgcgcgcgtt tctgcgcgaa
     4561 aacggttcta tatgttttat gtttatttaa ttctgtttga ctgtcgtatt tgcactttgt
     4621 tgctaataaa agatgtttac tttgttgtta atattgttgt acatgttatt ttgttattgt
     4681 tttattgttt ggtattcctt tctaaacggc ttaggcacaa gccaacagtt tggtgacgct
     4741 gtctgagctt acgtcataat ttttaaatta ttcacggtaa taccggatac cgaggtaaaa
     4801 tagggaggcg gtttgacggt gtcaaaattt ggataccgcc caagcctact atgtatgggc
     4861 ccagagacgg agtcactgcc aaccgagacg gtcatagctg tttcctgtgt gccgcttcct
     4921 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa
     4981 aggcggtaat acggttaccc acagaatcag gggataacgc aggaaagaac atgtgagcaa
     5041 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc
     5101 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
     5161 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc
     5221 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
     5281 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct
     5341 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
     5401 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
     5461 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
     5521 acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa
     5581 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt
     5641 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta
     5701 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat
     5761 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa
     5821 gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct
     5881 cagcgatctg tctctttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta
     5941 cgatacggga gggcttacca tctggcccca gtgctgcaat aataccgcgg gacccacgct
     6001 caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg
     6061 gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa
     6121 gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat cgctacaggc atcgtggtat
     6181 cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta
     6241 catgatcccc catgttgcgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca
     6301 gaagtaagtt ggccgccgtg ttatcactca tggttatggc agcactacat aattctctta
     6361 ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct
     6421 gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg
     6481 cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac
     6541 tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact
     6601 gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa
     6661 atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt
     6721 ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat
     6781 gtatttaga
//
