LOCUS       sequence-443473-       10027 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  GAL7(p)-amdS:mNeonGreen fusion reporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10027)
  AUTHORS   Phillips-Rose LS, Yu CK, West NP, Fraser JA
  TITLE     A Chimeric ORF Fusion Phenotypic Reporter for Cryptococcus 
            neoformans.
  JOURNAL   J Fungi (Basel). 2024 Aug 12;10(8):567. doi: 10.3390/jof10080567.
  PUBMED    39194893
REFERENCE   2  (bases 1 to 10027)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Fungi 
            (Basel)."; date: "2024-08-12"; volume: "10(8):567. doi"; pages: " 
            10.3390/jof10080567"
COMMENT     Sequence Label: pLSPR28
FEATURES             Location/Qualifiers
     source          1..10027
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(1824..2624)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MSMIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQG
                     RPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL
                     LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEE
                     HQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQD
                     IALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(1935..1954)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     2545..2564
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     3714..3736
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3728..3745
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3729..3745
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3755..3774
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        3755..3773
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      6720..6729
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(7839..7859)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(7839..7857)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(7878..7894)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7878..7894)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7891..7913)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7902..7918
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7926..7932,7933..7950,7951..7956))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7971..7992
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8109..8126)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8280..8868)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8360..8379)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9039..9830,9831..9899))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9662..9681
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9900..10004)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctgacgcgcc ctgtagcggc aagatctgcc catagacttc aaatcatgga attggacgtt
       61 tatcttcttg ctttggatat ccaccctatc gtacaatctt ggcactttgg atcatacgag
      121 gcactttgcg gactagggaa tggttatcat caatttcttg gacctctcca tgacacctgg
      181 catcgtcgag ctaccacagc cccctgaatt cttaaaggcg aacccccctt cctaggaacc
      241 ttgagcgctg gactggatgg cactctgaga ccgcctatgc ttaaggacgc gagcttaccc
      301 ttccattgat ggaagtcgat catttatgat aatagaacac ggcggaactt gactttacag
      361 gccaagggaa gcaacaggag aagaagctaa ggttataaca ggctcagcat atttgtatat
      421 acggtgccat tcaacgctga acactttgta ctcacggtct gcccgttgaa taacaccatc
      481 tgctgatgat catcccaatt tgtgagtctt atttgccatt gatcctggtg tataaccgat
      541 actgaagccc tttaggtctg atcagttata gacgtggtat attcattctt cctagattag
      601 ctatgccgaa agagatggat accttgatgt gactggaatg ccatgaagca ggagattgta
      661 gcagctcaaa ttggagaagg atgttgcaac cgtcggccgt gaaggtcttg tcatatcttt
      721 gtctgcctgg gagaatatgt acaagtgccc cgatgttact gatggaaaac atcgtgtggc
      781 gcgccacgat acttgtgtta attaaaccaa accagtctgt gccaagtgga ttgcttatgc
      841 atcgagtggc gagcccactg acctctgggc cccttttttg aactataaaa agcggccaag
      901 tctgatttcg gtctcacaat catcaacatc gacttctctt ttcgcctcga tcaacctaaa
      961 caacggtcac tcagccatga actggacaat ggaatatttc gccttgcctt ggatctttgg
     1021 cctctagact caatcaacgt caggataaag gtgagcatag gttgctcact cactgtagcc
     1081 tttttttccc ttcttcttgg tccagatggt tcccaaggct ctataaagtg tatctgtcag
     1141 ttccacttcc ttactccgaa gcgattcaga gttgtaaagt gcgtctgacc ccctgattat
     1201 atctcaaagt cccttcacaa attcagacat gatgcgataa agtgccggat tgtcattcgc
     1261 gtcttgtgac tgtatagaaa gtgccggcat cctcttttct tttcctcttt ggccgagcga
     1321 ctcagagttg tgaagcctgc tggtatctgt ctgttccctt gccctgataa agaatatcga
     1381 agagcatttc tttgattcac aatacgttga cgaatggtgt gatggatcct aagcagcgcc
     1441 agccctttga acttgtgtcg aaaaaaaaaa aaaaacagca tatgatacgt aaagggaaga
     1501 aaaggatgga gctgactgtt ttgacaggca taattttgag cgtgcttcat tggccggttt
     1561 atctgtatta acacggaaga gatgtagaaa ctagcttcct ggtttcagag acaaggagcc
     1621 atgaagatcc tgaggattca acggcgttgc tgagggtggg aaggatggta ttgttgtctg
     1681 tagatagatt aatgggcata taatgtcaaa aaagatgatt gacacgatgt ctgtctcgaa
     1741 ttcgcagtgt gctaaaattg tatcacttct ctaatatgta tatatacacc ctctaaggaa
     1801 aattaacccc ttaccgcctt cactcagaag aactcgtcaa gaaggcgata gaaggcgatg
     1861 cgctgcgaat cgggagcggc gataccgtaa agcacgagga agcggtcagc ccattcgccg
     1921 ccaagctctt cagcaatatc acgggtagcc aacgctatgt cctgatagcg gtccgccaca
     1981 cccagccggc cacagtcgat gaatccagaa aagcggccat tttccaccat gatattcggc
     2041 aagcaggcat cgccatgggt cacgacgaga tcctcgccgt cgggcatgcg cgccttgagc
     2101 ctggcgaaca gttcggctgg cgcgagcccc tgatgctctt cgtccagatc atcctgatcg
     2161 acaagaccgg cttccatccg agtacgtgct cgctcgatgc gatgtttcgc ttggtggtcg
     2221 aatgggcagg tagccggatc aagcgtatgc agccgccgca ttgcatcagc catgatggat
     2281 actttctcgg caggagcaag gtgggatgac aggagatcct gccccggcac ttcgcccaat
     2341 agcagccagt cccttcccgc ttcagtgaca acgtcgagca cagctgcgca aggaacgccc
     2401 gtcgtggcca gccacgatag ccgcgctgcc tcgtcctgca gttcattcag ggcaccggac
     2461 aggtcggtct tgacaaaaag aaccgggcgc ccctgcgctg acagccggaa cacggcggca
     2521 tcagagcagc cgattgtctg ttgtgcccag tcatagccga atagcctctc cacccaagcg
     2581 gccggagaac ctgcgtgcaa tccatcttgt tcaatcatag acatgttggg cgagtttact
     2641 aatggaaaaa gacaattagc aaagaggctg catattagga gacgcggtga agattgggga
     2701 ggacaatagg gtccgtgcac caaaaagtcc tgacggcagc tctgttcaac gcacatccat
     2761 ctcttccagc tatgcgtgtg gcaccagcac ggatggattg ttgcaccaaa aggacaaggc
     2821 gagatggatg caggtcgagg cgatgcggtg ctatcggccc tcgttgtcga acgccacgtc
     2881 gtcctctcgg cgaccgtcaa tcctggaagg aaatgaccaa gcgtgcaccc agcatccagc
     2941 atccaacatc cagcacccag cacctagcac ccatgatcca atggccggca tgcagatcag
     3001 cgtggggaga gcaccatcta gcagcccctg gcaggcccag cattgtgctc ggatgtgcga
     3061 taggcggtgg gtgcgataag gtggattagt ggaggatgga ggactgagtg gcgggcaagg
     3121 gaagggggcg ggaagatgac tcaccttggg cagtgggtgg gggatgcggt ataggggatg
     3181 tatgtggatg ctggcggagg atagaagctg taagctgagc tggtgccaac ggtgacgctg
     3241 tgagagtggt ctcggcgacc ctgacggcga ctcggcgtta ccccgtgtgg cacaccggac
     3301 gcgcgtcgag tgacgttgct gctattgttg ttaccatcat cctctcctcc gacaaccata
     3361 cactcagtgc acaccatgcc actcgaatcc tgcatgctta tgtgagtcct ccccttcccg
     3421 tgcgccgctc tccagctcac atcctcgcag ccatggactc aaccctatct cggtctattc
     3481 ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaaat gagctgattt
     3541 aacaaaaatt taacgcgaat tttaacaaaa tattaacgct tacaatttcc attcgccatt
     3601 caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat tacgccagct
     3661 ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt tttcccagtc
     3721 acgacgttgt aaaacgacgg ccagtgagcg cgcgtaatac gactcactat agggcgaatt
     3781 gggtaccggg ccccccctcg atagtgaagt cgtattgtct ctgcaatgta attctctttg
     3841 ccatgcatat gcttgagcct gtgccttctc tttaatcgac agcactgaca atctcttgca
     3901 gtttcgtcgc agtagtcacg cgtatatgtt tccatagttc cggcgggaag ttatcttact
     3961 aaatacggta tcgaaggaat tacacaatat ttgtactccg gcggtggaaa caggtcttgt
     4021 cgaaccctga tgaagcgaaa gctaagaggt tcgaaagtag caaaatcata ctttaaaatc
     4081 ttttatacgc gagtccaact tcgctgacaa gaaaatggta gtgggccttg cgaatgaatg
     4141 aggagagaaa gttttagctg tggattccca gcaggcctcc ttacttaagt ttgattaaaa
     4201 attattacga aaagatatat atagtaataa atttgaaatg aactaatcat aatcgaaaaa
     4261 ttcccgttca acataaattc ccgttcattt aaattcccgt tcaacataaa ttcccgttca
     4321 tttaaattcc cgttcaacat aaaattcccg ttcataaatt cccatggcat tcatcatcat
     4381 gaatcctttg ttgtatagtt attatataac ggatattccc gttcgtatcc attgcatatc
     4441 ttatcgcata gtcttcccgt tcatcgtctc atcatcaagt tattgattgt caattcacgc
     4501 ttacatattc ccgttcttct gtctaccatc tatctatcac atcatctgtg aattcttccc
     4561 gtttttatgg gaagactgtc gtcatgttgt caattgagca gcagacataa tcgatgctat
     4621 atatctagag cttcacggct caggctgaac ttggattaat gtggactact ttcacaacga
     4681 atacttatca gcactcaatt ctctcctgag aatgcctcag tcctgggagg agctcgccgc
     4741 tgacaagcgt gcccgtctcg ctaagaccat ccctgacgag tggaaggtcc agaccctccc
     4801 tgccgaggac tccgttattg acttccctaa gaagtctggc atcctttctg aggccgagct
     4861 caagatcact gaggcctccg ctgctgacct tgtctccaag ctcgccgccg gtgagttgac
     4921 ctctgtcgag gttacccttg ctttctgtaa gcgagctgct atcgcccagc agttgactaa
     4981 ctgcgcccac gagttcttcc ctgacgccgc tctcgcccag gctagggagc tcgacgagta
     5041 ctacgctaag cacaagaggc ccgttggtcc tctccacggc ctccccatct ctctcaagga
     5101 ccagcttcga gtcaagggct acgagacttc tatgggctac atctcttggc ttaacaagta
     5161 cgacgagggc gactctgttc tcactaccat gctccgtaag gccggtgccg tcttctacgt
     5221 caagacctct gtcccccaga ccctcatggt ctgcgagact gtcaacaaca tcatcggccg
     5281 taccgtcaac cctcgtaaca agaactggtc ttgcggcggc tcctctggtg gtgagggtgc
     5341 catcgttggc attcgtggtg gcgtcatcgg tgttggtacc gacattggtg gctctattcg
     5401 agtccccgcc gccttcaact tcctctacgg tcttaggccc tcccacggcc gactccccta
     5461 cgctaagatg gccaactcca tggagggtca ggagaccgtc cactccgttg tcggccccat
     5521 tacccactct gttgaggacc tccgtctctt caccaagtcc gtcctcggtc aggagccttg
     5581 gaagtacgac tccaaggtca tccccatgcc ctggcgtcag tccgagtctg acattattgc
     5641 ctccaagatc aagaacggcg gcctcaacat cggctactac aacttcgacg gcaacgtcct
     5701 tcctcaccct cctatcctcc gtggcgtcga gaccaccgtc gccgctctcg ccaaggccgg
     5761 tcacaccgtc accccctgga ccccttacaa gcacgacttc ggccacgacc tcatctccca
     5821 catctacgcc gctgacggct ccgccgacgt tatgcgtgac atctccgctt ccggcgagcc
     5881 cgccattcct aacatcaagg accttctcaa ccccaacatc aaggctgtta acatgaacga
     5941 gctctgggac acccacctcc agaagtggaa ctaccagatg gagtaccttg agaagtggcg
     6001 agaggctgag gagaaggccg gcaaggaggt gagtagatca caatttgtaa caggttggat
     6061 gataagtccc cggtctaaca tgtagcgacg acactgaagc tcacttcttg tagctcgacg
     6121 ccatcatcgc ccccattacc cctaccgctg ccgttcgaca cgaccagttc cgatactacg
     6181 gctacgcctc tgtcatcaac ctcctcgact tcacctccgt cgttgttccc gttaccttcg
     6241 ccgacaagaa catcgacaag aagaacgagt ccttcaaggc cgtttccgag cttgacgccc
     6301 tcgtccagga ggagtacgac cccgaggcct accacggcgc tcccgttgct gtccaggtta
     6361 tcggtcgaag gctctccgag gagaggacct tggccattgc tgaggaggtc ggcaagttgc
     6421 tcggtaacgt cgtcactcct ggttctggcg gtggtggctc cggcatggtc tccaagggcg
     6481 aggaggacaa catggcctct ctccctgcca ctcacgagtt gcacatcttc ggctccatca
     6541 acggtgtcga cttcgacatg gtcggtcagg gcaccggcaa ccctaacgac ggttacgagg
     6601 agttgaacct taagtccacc aagggtgacc tccagttctc cccctggatt cttgtccctc
     6661 acatcggcta cggcttccac cagtaccttc cctaccctga cggcatgtcc cctttccagg
     6721 ccgccatggt tgacggctcc ggttaccagg tccaccgtac tatgcagttc gaggacggtg
     6781 cctcccttac tgttaactac cgttacacct acgagggttc ccacatcaag ggtgaggccc
     6841 aggtcaaggg cactggtttc cctgctgacg gtcctgtcat gaccaactcc cttaccgctg
     6901 ccgactggtg caggtccaag aagacttacc ccaacgacaa gaccatcatc tccaccttca
     6961 agtggtccta caccactggt aacggcaagc gttaccgatc cactgcccga accacctaca
     7021 ccttcgccaa gcctatggcc gctaactacc ttaagaacca gcccatgtac gttttccgta
     7081 agaccgagct caagcactcc aagaccgagc tcaacttcaa ggagtggcag aaggccttca
     7141 ccgacgtcat gggcatggac gagctttaca agtaaaaggt tgagcgcgta atagtgggtt
     7201 ggtcttgtct ctatccattc atctgtgaga catactgatt tcaacttttt catcagataa
     7261 taaggatggt gtgagataag caaggctggg ttatgaggtt tgggactaga tggtgtttcg
     7321 cgtctaaagg tgagtcttcg ttggttcaat cacattgatc tgatcgctga tcaattgtca
     7381 gcctctgaat gtgctcttga agaaaatggt tgtctgtaag aatccagaac caaaaacggt
     7441 catttcaagt atgtatcctc cgtgaagttc ctttagctct tgctaatatt ggactttagc
     7501 gtgctttagc caaagacagg gtcgcgaggt taagaggatg ggccaggaaa aatctaagtt
     7561 agatttgtca cacaaagaaa aagattgtct cagttttggt tttgtttggt ttgatttatg
     7621 cataggaaat atttatgatc gaaatcactc atttcctgct tcgtaaatat catctactat
     7681 atgcttcgta aatgtccatc tcctacatat tgtggatatg ataaatgcaa tcctctcgtc
     7741 tacactctcg gcttatgcct ttgtcttctt tgcgctcctt ttagccttct ttttattcct
     7801 agtcttcaag cccatcggtg gagctccagc ttttgttccc tttagtgagg gttaattgcg
     7861 cgcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt
     7921 ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc
     7981 taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc
     8041 cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct
     8101 tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca
     8161 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac
     8221 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt
     8281 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
     8341 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
     8401 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
     8461 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
     8521 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
     8581 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
     8641 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
     8701 aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc
     8761 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt
     8821 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg
     8881 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc
     8941 atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa
     9001 tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag
     9061 gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg
     9121 tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga
     9181 gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag
     9241 cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa
     9301 gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc
     9361 atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca
     9421 aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg
     9481 atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat
     9541 aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc
     9601 aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg
     9661 gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg
     9721 gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt
     9781 gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca
     9841 ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata
     9901 ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac
     9961 atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa
    10021 gtgccac
//
