LOCUS       sequence-443474-       10342 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  TEF1(p)-mRuby3:amdS fusion reporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10342)
  AUTHORS   Phillips-Rose LS, Yu CK, West NP, Fraser JA
  TITLE     A Chimeric ORF Fusion Phenotypic Reporter for Cryptococcus 
            neoformans.
  JOURNAL   J Fungi (Basel). 2024 Aug 12;10(8):567. doi: 10.3390/jof10080567.
  PUBMED    39194893
REFERENCE   2  (bases 1 to 10342)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Fungi 
            (Basel)."; date: "2024-08-12"; volume: "10(8):567. doi"; pages: " 
            10.3390/jof10080567"
COMMENT     Sequence Label: pLSPR31
FEATURES             Location/Qualifiers
     source          1..10342
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(1824..2624)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MSMIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQG
                     RPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL
                     LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEE
                     HQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQD
                     IALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(1935..1954)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     2545..2564
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     3714..3736
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3728..3745
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3729..3745
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3755..3774
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        3755..3773
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8154..8174)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(8154..8172)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(8193..8209)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8193..8209)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8206..8228)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8217..8233
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8241..8247,8248..8265,8266..8271))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8286..8307
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8424..8441)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8595..9183)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8675..8694)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9354..10145,10146..10214))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9977..9996
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10215..10319)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctgacgcgcc ctgtagcggc aagatctgcc catagacttc aaatcatgga attggacgtt
       61 tatcttcttg ctttggatat ccaccctatc gtacaatctt ggcactttgg atcatacgag
      121 gcactttgcg gactagggaa tggttatcat caatttcttg gacctctcca tgacacctgg
      181 catcgtcgag ctaccacagc cccctgaatt cttaaaggcg aacccccctt cctaggaacc
      241 ttgagcgctg gactggatgg cactctgaga ccgcctatgc ttaaggacgc gagcttaccc
      301 ttccattgat ggaagtcgat catttatgat aatagaacac ggcggaactt gactttacag
      361 gccaagggaa gcaacaggag aagaagctaa ggttataaca ggctcagcat atttgtatat
      421 acggtgccat tcaacgctga acactttgta ctcacggtct gcccgttgaa taacaccatc
      481 tgctgatgat catcccaatt tgtgagtctt atttgccatt gatcctggtg tataaccgat
      541 actgaagccc tttaggtctg atcagttata gacgtggtat attcattctt cctagattag
      601 ctatgccgaa agagatggat accttgatgt gactggaatg ccatgaagca ggagattgta
      661 gcagctcaaa ttggagaagg atgttgcaac cgtcggccgt gaaggtcttg tcatatcttt
      721 gtctgcctgg gagaatatgt acaagtgccc cgatgttact gatggaaaac atcgtgtggc
      781 gcgccacgat acttgtgtta attaaaccaa accagtctgt gccaagtgga ttgcttatgc
      841 atcgagtggc gagcccactg acctctgggc cccttttttg aactataaaa agcggccaag
      901 tctgatttcg gtctcacaat catcaacatc gacttctctt ttcgcctcga tcaacctaaa
      961 caacggtcac tcagccatga actggacaat ggaatatttc gccttgcctt ggatctttgg
     1021 cctctagact caatcaacgt caggataaag gtgagcatag gttgctcact cactgtagcc
     1081 tttttttccc ttcttcttgg tccagatggt tcccaaggct ctataaagtg tatctgtcag
     1141 ttccacttcc ttactccgaa gcgattcaga gttgtaaagt gcgtctgacc ccctgattat
     1201 atctcaaagt cccttcacaa attcagacat gatgcgataa agtgccggat tgtcattcgc
     1261 gtcttgtgac tgtatagaaa gtgccggcat cctcttttct tttcctcttt ggccgagcga
     1321 ctcagagttg tgaagcctgc tggtatctgt ctgttccctt gccctgataa agaatatcga
     1381 agagcatttc tttgattcac aatacgttga cgaatggtgt gatggatcct aagcagcgcc
     1441 agccctttga acttgtgtcg aaaaaaaaaa aaaaacagca tatgatacgt aaagggaaga
     1501 aaaggatgga gctgactgtt ttgacaggca taattttgag cgtgcttcat tggccggttt
     1561 atctgtatta acacggaaga gatgtagaaa ctagcttcct ggtttcagag acaaggagcc
     1621 atgaagatcc tgaggattca acggcgttgc tgagggtggg aaggatggta ttgttgtctg
     1681 tagatagatt aatgggcata taatgtcaaa aaagatgatt gacacgatgt ctgtctcgaa
     1741 ttcgcagtgt gctaaaattg tatcacttct ctaatatgta tatatacacc ctctaaggaa
     1801 aattaacccc ttaccgcctt cactcagaag aactcgtcaa gaaggcgata gaaggcgatg
     1861 cgctgcgaat cgggagcggc gataccgtaa agcacgagga agcggtcagc ccattcgccg
     1921 ccaagctctt cagcaatatc acgggtagcc aacgctatgt cctgatagcg gtccgccaca
     1981 cccagccggc cacagtcgat gaatccagaa aagcggccat tttccaccat gatattcggc
     2041 aagcaggcat cgccatgggt cacgacgaga tcctcgccgt cgggcatgcg cgccttgagc
     2101 ctggcgaaca gttcggctgg cgcgagcccc tgatgctctt cgtccagatc atcctgatcg
     2161 acaagaccgg cttccatccg agtacgtgct cgctcgatgc gatgtttcgc ttggtggtcg
     2221 aatgggcagg tagccggatc aagcgtatgc agccgccgca ttgcatcagc catgatggat
     2281 actttctcgg caggagcaag gtgggatgac aggagatcct gccccggcac ttcgcccaat
     2341 agcagccagt cccttcccgc ttcagtgaca acgtcgagca cagctgcgca aggaacgccc
     2401 gtcgtggcca gccacgatag ccgcgctgcc tcgtcctgca gttcattcag ggcaccggac
     2461 aggtcggtct tgacaaaaag aaccgggcgc ccctgcgctg acagccggaa cacggcggca
     2521 tcagagcagc cgattgtctg ttgtgcccag tcatagccga atagcctctc cacccaagcg
     2581 gccggagaac ctgcgtgcaa tccatcttgt tcaatcatag acatgttggg cgagtttact
     2641 aatggaaaaa gacaattagc aaagaggctg catattagga gacgcggtga agattgggga
     2701 ggacaatagg gtccgtgcac caaaaagtcc tgacggcagc tctgttcaac gcacatccat
     2761 ctcttccagc tatgcgtgtg gcaccagcac ggatggattg ttgcaccaaa aggacaaggc
     2821 gagatggatg caggtcgagg cgatgcggtg ctatcggccc tcgttgtcga acgccacgtc
     2881 gtcctctcgg cgaccgtcaa tcctggaagg aaatgaccaa gcgtgcaccc agcatccagc
     2941 atccaacatc cagcacccag cacctagcac ccatgatcca atggccggca tgcagatcag
     3001 cgtggggaga gcaccatcta gcagcccctg gcaggcccag cattgtgctc ggatgtgcga
     3061 taggcggtgg gtgcgataag gtggattagt ggaggatgga ggactgagtg gcgggcaagg
     3121 gaagggggcg ggaagatgac tcaccttggg cagtgggtgg gggatgcggt ataggggatg
     3181 tatgtggatg ctggcggagg atagaagctg taagctgagc tggtgccaac ggtgacgctg
     3241 tgagagtggt ctcggcgacc ctgacggcga ctcggcgtta ccccgtgtgg cacaccggac
     3301 gcgcgtcgag tgacgttgct gctattgttg ttaccatcat cctctcctcc gacaaccata
     3361 cactcagtgc acaccatgcc actcgaatcc tgcatgctta tgtgagtcct ccccttcccg
     3421 tgcgccgctc tccagctcac atcctcgcag ccatggactc aaccctatct cggtctattc
     3481 ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaaat gagctgattt
     3541 aacaaaaatt taacgcgaat tttaacaaaa tattaacgct tacaatttcc attcgccatt
     3601 caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat tacgccagct
     3661 ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt tttcccagtc
     3721 acgacgttgt aaaacgacgg ccagtgagcg cgcgtaatac gactcactat agggcgaatt
     3781 gggtaccggg ccccccctcg acattctatg ctatacggta caagattgtg gctactatgc
     3841 aagctccata gcgaatgatc ttacactgac cttgtcgacc gttctgaatg attaacaatg
     3901 gtcgcaagtg tggttgaccc acctgcgggc gcttcttaac tatctatcat cggtatgtca
     3961 agtatcactc cctttaaaca aaagaataag agcataatac agcagtctca tcatggcaac
     4021 ccttcgcgca atgtacaaaa agcagccaga agcccccacg cacaagcaat aatgtcgtgt
     4081 tctaccctgc catgaacaag cgtcatggcc attagcgaac gccccaatat tcagccccat
     4141 tcgccaagca agttgcctcg cccccttccc agaagatcat gggcatctcg tcttcttcta
     4201 atttcgcatt ttgtgaagct ccattcatgc acgtcggctt ctggacgact cttatcatat
     4261 gcattggcct gctcggtgtg aggatgagcc actcccacat gtttagacgg caggggcctc
     4321 ttgccacccg ttgaattttt ggtgcgaaga cgtgcctggg aacgacctcc actttaaatt
     4381 tctccgttgt tgcggtcgct ccatcctcct cacatttctt gcctcctaag tcccatgtgc
     4441 cacactcggt gaaaaacgga gtttgatacg gatgaaattt ccagtgagct tttcacattc
     4501 tggaggtttc gaaaaaaatt gtgggatttt ctgaattaat ccgagaatcc gccctgccca
     4561 tcaaccggaa agaattaaat ccctcgcaca ttcctttctc ccaccacctt ctttatctct
     4621 acattcttta accaaaacac ctaggtgagt acccaaagtc ttttgacacc tttgaaaatc
     4681 cgcgccagtc gtagggcacg taaaacaatt ctcgctggtg gcttctgcag gtgaagtcgg
     4741 ggcagagtgg agctgagcat ggtcgaccac ccacatcatt ctgtcttgtt tttggcgatg
     4801 tcttccatgc ttttgccctc atctgttgcg tcgactgcag agtactgttt tgccactact
     4861 gagctcaaca tgggcttgtg tgtggtgaag aaaaaaattt ggtggctctt gaaaattttc
     4921 aacccatcgg atacatgccc ttccaactca caacgcccta acactttatt gtcctatcat
     4981 tgcctcttcg cacatgctaa cgatctccac agaaaacttc aaaatggtct ctaagggcga
     5041 ggagctcatc aaggagaaca tgcgtatgaa ggtcgtcatg gagggttctg tcaacggcca
     5101 ccagttcaag tgcactggtg agggtgaggg caggccctac gagggtgtcc agaccatgag
     5161 gatcaaggtc atcgagggtg gtcccctccc tttcgccttc gacattcttg ccacttcttt
     5221 catgtacggc tcccgtacct tcatcaagta ccccgccgac atccctgact tcttcaagca
     5281 gtccttccct gagggtttca cttgggagag ggttaccagg tacgaggacg gtggtgtcgt
     5341 caccgtcacc caggacacct cccttgagga cggcgagctc gtctacaacg tcaaggtcag
     5401 gggcgttaac ttcccctcca acggtcccgt catgcagaag aagaccaagg gttgggagcc
     5461 taacactgag atgatgtacc ctgctgacgg tggtctcagg ggttacactg acatcgctct
     5521 caaggttgac ggtggtggcc acctccactg caacttcgtc actacttaca ggtctaagaa
     5581 gaccgtcggc aacatcaaga tgcccggtgt ccacgccgtt gaccaccgtc tcgagaggat
     5641 cgaggagtcc gacaacgaga cctacgttgt ccagagggag gtcgctgttg ccaagtactc
     5701 caaccttggt ggtggcatgg acgagctcta caagggttct ggcggtggtg gctccggcat
     5761 gcctcagtcc tgggaggagc tcgccgctga caagcgtgcc cgtctcgcta agaccatccc
     5821 tgacgagtgg aaggtccaga ccctccctgc cgaggactcc gttattgact tccctaagaa
     5881 gtctggcatc ctttctgagg ccgagctcaa gatcactgag gcctccgctg ctgaccttgt
     5941 ctccaagctc gccgccggtg agttgacctc tgtcgaggtt acccttgctt tctgtaagcg
     6001 agctgctatc gcccagcagt tgactaactg cgcccacgag ttcttccctg acgccgctct
     6061 cgcccaggct agggagctcg acgagtacta cgctaagcac aagaggcccg ttggtcctct
     6121 ccacggcctc cccatctctc tcaaggacca gcttcgagtc aagggctacg agacttctat
     6181 gggctacatc tcttggctta acaagtacga cgagggcgac tctgttctca ctaccatgct
     6241 ccgtaaggcc ggtgccgtct tctacgtcaa gacctctgtc ccccagaccc tcatggtctg
     6301 cgagactgtc aacaacatca tcggccgtac cgtcaaccct cgtaacaaga actggtcttg
     6361 cggcggctcc tctggtggtg agggtgccat cgttggcatt cgtggtggcg tcatcggtgt
     6421 tggtaccgac attggtggct ctattcgagt ccccgccgcc ttcaacttcc tctacggtct
     6481 taggccctcc cacggccgac tcccctacgc taagatggcc aactccatgg agggtcagga
     6541 gaccgtccac tccgttgtcg gccccattac ccactctgtt gaggacctcc gtctcttcac
     6601 caagtccgtc ctcggtcagg agccttggaa gtacgactcc aaggtcatcc ccatgccctg
     6661 gcgtcagtcc gagtctgaca ttattgcctc caagatcaag aacggcggcc tcaacatcgg
     6721 ctactacaac ttcgacggca acgtccttcc tcaccctcct atcctccgtg gcgtcgagac
     6781 caccgtcgcc gctctcgcca aggccggtca caccgtcacc ccctggaccc cttacaagca
     6841 cgacttcggc cacgacctca tctcccacat ctacgccgct gacggctccg ccgacgttat
     6901 gcgtgacatc tccgcttccg gcgagcccgc cattcctaac atcaaggacc ttctcaaccc
     6961 caacatcaag gctgttaaca tgaacgagct ctgggacacc cacctccaga agtggaacta
     7021 ccagatggag taccttgaga agtggcgaga ggctgaggag aaggccggca aggaggtgag
     7081 tagatcacaa tttgtaacag gttggatgat aagtccccgg tctaacatgt agcgacgaca
     7141 ctgaagctca cttcttgtag ctcgacgcca tcatcgcccc cattacccct accgctgccg
     7201 ttcgacacga ccagttccga tactacggct acgcctctgt catcaacctc ctcgacttca
     7261 cctccgtcgt tgttcccgtt accttcgccg acaagaacat cgacaagaag aacgagtcct
     7321 tcaaggccgt ttccgagctt gacgccctcg tccaggagga gtacgacccc gaggcctacc
     7381 acggcgctcc cgttgctgtc caggttatcg gtcgaaggct ctccgaggag aggaccttgg
     7441 ccattgctga ggaggtcggc aagttgctcg gtaacgtcgt cactccttag aaggttgagc
     7501 gcgtaatagt gggttggtct tgtctctatc cattcatctg tgagacatac tgatttcaac
     7561 tttttcatca gataataagg atggtgtgag ataagcaagg ctgggttatg aggtttggga
     7621 ctagatggtg tttcgcgtct aaaggtgagt cttcgttggt tcaatcacat tgatctgatc
     7681 gctgatcaat tgtcagcctc tgaatgtgct cttgaagaaa atggttgtct gtaagaatcc
     7741 agaaccaaaa acggtcattt caagtatgta tcctccgtga agttccttta gctcttgcta
     7801 atattggact ttagcgtgct ttagccaaag acagggtcgc gaggttaaga ggatgggcca
     7861 ggaaaaatct aagttagatt tgtcacacaa agaaaaagat tgtctcagtt ttggttttgt
     7921 ttggtttgat ttatgcatag gaaatattta tgatcgaaat cactcatttc ctgcttcgta
     7981 aatatcatct actatatgct tcgtaaatgt ccatctccta catattgtgg atatgataaa
     8041 tgcaatcctc tcgtctacac tctcggctta tgcctttgtc ttctttgcgc tccttttagc
     8101 cttcttttta ttcctagtct tcaagcccat cggtggagct ccagcttttg ttccctttag
     8161 tgagggttaa ttgcgcgctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt
     8221 tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt
     8281 gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg
     8341 ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg
     8401 cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg
     8461 cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat
     8521 aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc
     8581 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc
     8641 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga
     8701 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt
     8761 ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg
     8821 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc
     8881 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg
     8941 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc
     9001 ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg
     9061 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc
     9121 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct
     9181 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt
     9241 taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa
     9301 aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa
     9361 tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc
     9421 tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct
     9481 gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca
     9541 gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt
     9601 aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt
     9661 gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc
     9721 ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc
     9781 tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt
     9841 atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact
     9901 ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc
     9961 ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt
    10021 ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg
    10081 atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct
    10141 gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa
    10201 tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt
    10261 ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc
    10321 acatttcccc gaaaagtgcc ac
//
