LOCUS       sequence-443475-       12328 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express METTL1-S27D gene in mesenchymal lineage of Zebrafish..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12328)
  AUTHORS   Ali RH, Orellana EA, Lee SH, Chae YC, Chen Y, Clauwaert J, Kennedy 
            AL, Gutierrez AE, Papke DJ, Valenzuela M, Silverman B, Falzetta A, 
            Ficarro SB, Marto JA, Fletcher CDM, Perez-Atayde A, Alcindor T, 
            Shimamura A, Prensner JR, Gregory RI, Gutierrez A
  TITLE     A methyltransferase-independent role for METTL1 in tRNA 
            aminoacylation and oncogenic transformation.
  JOURNAL   Mol Cell. 2025 Mar 6;85(5):948-961.e11. doi: 
            10.1016/j.molcel.2025.01.003. Epub 2025 Jan 31.
  PUBMED    39892392
REFERENCE   2  (bases 1 to 12328)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2025.01.003"; journalName: "Mol Cell"; date: 
            "2025-03-6- 6"; volume: "85"; issue: "5"; pages: "948-961.e11"
COMMENT     Sequence Label: rag2:METTL1-S27D_CE_pISceI
FEATURES             Location/Qualifiers
     source          1..12328
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    6721..6855
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(join(7157..7870,7871..7873,7874..7876))
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             complement(join(7160..7870,7871..7873,7874..7876))
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(7202..7223)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     7551..7570
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     7810..7831
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(9057..9073)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9057..9073)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     promoter        complement(9128..9146)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(9156..9173)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(9156..9172)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9165..9187)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      complement(9313..9768)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9450..9471)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     9662..9681
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        9795..9899
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9900..9968,9969..10760)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10118..10137)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10931..11519
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11420..11439
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     11673..11690
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    11807..11828
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(11843..11848,11849..11866,11867..11873)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11881..11897
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     11886..11908
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     11905..11921
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     11905..11921
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     11940..11960
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        11942..11960
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 tctcttcatg gccctgctct tcagacacag gtgctccgct gtttgtgttt ttcactgttc
       61 ttcaggcact cgtggttacg ggtttttgtt ttgcactctt gcacacacat ctctcggcct
      121 ccaaaacaaa cacacaactg cacgagggct cgaagccccc accgaaaaac aaaaacatta
      181 tgctaatgag gaaggcacag ctgttccctt cttcactctc tctctcgttc tttcaacaac
      241 agtcgcctaa tttctctcgt tcctttaaag agccgccaag cagaaacagt taatgcaaat
      301 ataagcctgc gggaaataag gagaattttt acaaaatccg agcttcccct cctgccaaaa
      361 tgtgatgtgt gactcacgca ttcgatattt ggtgtaatga gagtgaatca ttctgctcca
      421 caccgaaccc gatgccacag accactcaga tgcacaccga ggagactatt ttaacagcac
      481 attacatcca gactgtcaca gatgcattac caagagtgtg atttcagtgt cgaatgcagg
      541 tcttaaagga aaatctgttg cgattgcatg tgcgctaact agacacgacc aacgaatggg
      601 aacaatctgt gaaatattat acaatcacag gcaattaaaa gatgtttaaa gttcaattta
      661 gttttaatta atatgttata taaaactaat ctgttttaaa tgtatattta tgattttagc
      721 tttagctagc ctcttgacca gcagctgaag tgtccggcaa gtacctaaaa gggagaccac
      781 atgggaaagc taggttgctc ccggaagagg tgttagtaaa ccagcaggtc tacctgtggt
      841 ctgtgtgggt cctaatgccc cagtataaac cagcaggtca acctgtggtc tgtgtgggtc
      901 ctaatgcccc agtataaacc agcaggtcta cctgtggtct gtgtgggtcc taatgcccca
      961 gtataaacca gcaggtcaac ctgtagtctg tgtgggtcct aatgccccag tataaaccag
     1021 caggtctacc tgtggtctgt gtgggtccta atgccccagt ataaaccaac aggtcaacct
     1081 gtgatctgtg tgggtcctaa tgccccagta taaaccagca ggtcaacctg tggtctgtgt
     1141 gggtcctaat gccccagtat aaaccagcag gtcaacctgt ggtctgtgtg ggtcctaatg
     1201 ccccagtata aaccagcagg tcaacctacc tgtggtctgt gtgggtccta atgccccagt
     1261 ataaaccagc aggtcaacct gtggtctgtg tgggtcctaa tgccccagta taaaccagca
     1321 ggtctacctg tggtctgtgt gggtcctaat gcaccagtat aaaccagcag gtcaacctgt
     1381 ggtctgtgtg ggttctaatg ccccagtata aaccagcagg tcaacctgtg gtctgtgtgg
     1441 gtccaaatgc cccagtacaa accagcaggt caacctgtgg tctgtgtggg tcctaatgcc
     1501 ccagtataaa ccagcaggtc aacctgtggt ctgtgtgggt cctaatgccc cggtacaaac
     1561 cagcatgtca acctgtggtc tgtgtgggtc ctaatgcccc agtataaacc agcaggtcaa
     1621 cctgtggtct gtgtgggtcc taatgcccca gtatattgat ggagactcta tactgctcgg
     1681 tgagctccat ctttcggata agatgttaaa acaaggtccc gactctctgt ggtggtaaaa
     1741 aattcccagg atgtccttcc taccctggca tcctggacaa atttgctcac tgtcctctgt
     1801 ccatcatggc ctcctaacca tccccatatc ataattggct ttatcactct agctggggcc
     1861 gaaatcgcat acttccattt tatgaggtac gctaaaatca gtatgcgagc caagtaatat
     1921 gtccgaatac ttccggcgag attctagagt gtgcattcga tgcacgctac gctattccat
     1981 gatgccccgt gagagaattc ttgaatcgga gagaagcaac gcaactcatg cgggtaggtt
     2041 acgtgacgat gacatattgg cggatgtagt acgtccaagt tccattcata ctgctcacat
     2101 tcatatttta tagaatgttc ttttcccaca gccaagtagt aagtttaaat tcaaatacag
     2161 tacctactaa gtagtagtag acggtttcgg acgcagcccc tgtctcctct ccaccaatat
     2221 actggtgtgt agtgtgcggt ctggtgcaat atggctgcca tcgcgtcatc caggtggatg
     2281 ctgcacactg gtggttgatg aggagatttt gcccaaaaaa gtgttttgag tttccagaaa
     2341 agcgcgatat aaatgtaagg aattatcatt agcttattat aatggaactt tctggataac
     2401 ttgtgccaga gctaggggcc attaaaactt tgccctgggg tacctcaggg agctgcaagg
     2461 aagtgctata gggctaatga aatgtttggg ggaaaaaaaa aaattttata agtaaaaata
     2521 aaactaaaat aattaataat ttcattgttg aaaattatag taaaattgat aaacaagata
     2581 agcagcatta gtataaaaaa ctaaaaacaa acgtaattta aaataattaa attgttttaa
     2641 ataaattata caaaataatg tttacaacga cacggtggtg cagtaggtag tgctgtcgcc
     2701 agcaaagcaa gaaggtcgct ggttcgagcc tcagctggat cacttgatgt ttctgtgtgg
     2761 agcattcgcg tgcgtttaac cccagattaa taaagagact aagccgaaaa gaaaataaat
     2821 gtatgaaaat gtttacgtaa aatgtaaata aatgcatgat taataaaata tatgaatttt
     2881 aaagattgag gtagaattgt tttattattc acacatttgg ttatttttaa tcagtgacaa
     2941 cttgtgatgc tctttatatt gtttattacg ctactttgaa tattcagtct caaattgcat
     3001 gtaaatacga cttcaaagca gaattagttt aatttaattg cctgtaaaca gtgttgtact
     3061 ttgatggtga ttgtctgata ataatatgat ttcactattt aaaatttgaa aaaaaaaaat
     3121 ctgaaaaata ctcaccggcc actttattag gtacacctgt ctaactgctt gttaacgtaa
     3181 atttctaatc agccaatcac caggcagcaa ctcagtgcat ttaggcatgt agacatggtc
     3241 aagacgatct gctgcagttc aaagcgagca tcagaatgga gaagcaaggt gatttaagtg
     3301 actttaaacg tgacgtgttt gttggtgcca gacgggctgg tctgagtatt tcagaaactg
     3361 ctgatttact ggggttttca cacacaaaca tctctggggt ttacagagaa tggtccaaaa
     3421 aaagataata tccagtgagc ggcagttctg tgggggcaaa tgtcttgttg atgccagagg
     3481 tcagaggaga atggcctgac tggttccagc tgatagaaag gcaacagtaa ttcaaataac
     3541 cactcgttag aactgaggca tgcagaagag cacctttgaa cacaacatgt cgaaccttga
     3601 ggcgggtggg ctacagcagc tcaagaccac atcgggtgcc actcctgtca gctaagaaca
     3661 ggaaactgag gctacaattc gcacaggctc aacataagta gacaatagaa gattgaacat
     3721 tatgttgcct ggtcagatgg atacattcta ccttgtatca acagttcagg ctggtgatgg
     3781 tggtgtaatg gtgtggggga tatattcttg gcacactttg ggcccattag taccaattaa
     3841 ccatcgtgtc aacgccacag cctaactgag tattgttgct gaccatgttc atttcttttt
     3901 gaccacagtg tacccatctt ctgatggcta cttcctgcag gataatgcac catgtcataa
     3961 agcgtgaatg atctcagact ggtttcttga acatgacaat gagttcactg tgctcgaatg
     4021 acctccacag tcaccagatc tcaatccaat agagcgcctt tggggtgtgg tagaatggaa
     4081 gattcgcatc ctggatgtgc agccgacaaa tctgcatgat gctagcatgt caatatggac
     4141 caaaatctct gaggaatttt ccagtacctg gttgaaatgc aaaaggaagg gaatctatgc
     4201 taaggcaaaa agaggtccaa cccagtacta gtggggtgta cataataaag tggccggtga
     4261 gtgtatattg ttaaggatat ctagataatc caaataattt acagcactat ttaaaaaaaa
     4321 aactttatgt caccaagtct gcatttattt gatcacaatt acagtcgaaa aagcaatatc
     4381 gggatacatt gtttccattt aaaataacta tattatattt taaatatttt tgacaatgtt
     4441 atttctttaa tgccaaagct aaattgtatt caatctgaat tttagtgtca catgatcctt
     4501 cagaaaatat taacacattg ctaaaaatgt atattattat caatttggat tttttttttt
     4561 tttttgtata ttaacctatg ctattggtta ctatacaaaa gtattactgt gaatatatgc
     4621 atatacagaa ccgtatcaag cgcggggggt ggttgggtta tgtccccatt tggaaagacc
     4681 tggaaacatt cataatgact aaaattgcac ggaaaaatat aatatctatg agtattgctg
     4741 atcagcagca caaaaccaga actccatatt aataaacagc caatgatgca gtgagtttgt
     4801 gcatgttttc attcaaactc gtgtgattgt gctcaaacac ctgttgtgag cagcagttgc
     4861 aaacagtaaa cctcaagagg agccagtcaa ccttcacaaa cactcaacct ctggctcact
     4921 cactaaacaa ttcgcgttaa tatctaaaat aaaaatttcc agcaccaaac agctctcaga
     4981 tttcggagta cactgaccaa gaggtaagaa gtaacgttac attactagga cgactattgt
     5041 aagactagat atgtaatatt tggaagcgta cgtgttttaa gtgacatata gagtagagga
     5101 actgatggaa atacgatttg cataatgtaa aatgatatat attaaaatat aggctacagt
     5161 aggtaacgtg gtttacatat aacgttactg tgttaatgta gtgctcagtt actcgtgttg
     5221 acagttatta ttagtgcttg ctaatttgtt tgttaatgct tctcttgcgt atttgattac
     5281 cttcaggcga atctaaaaca tatcagatgt agaaacggca gaaaaatgtt gaagaaaata
     5341 cgagaatagt agcttttgga gcacatagaa aagtacatca gataaagcct accgtttaaa
     5401 cgcagacgcc gctctcgcac tgaatgctgg gtactgtagt ccttatattg tatgcttaat
     5461 gtgcgtctca acgggaaact agagttagga aaactacaat ttcctgtgga aaatgtttct
     5521 gttttcgggg aaacagcgaa agaaagatat ggttccgcgt ttttttctta ctttgaataa
     5581 taaaataaga cctaactaca cacttcgctt actcttatgc taatttgaag cactagcatt
     5641 ctttgataca atgataatca tttaaacatc acagttatac tacaaatact atggttaaac
     5701 tatgtctagt gtagtgtaac actatggttg ccattgttat ctttaagttt aagtattatc
     5761 tgtggtaaag ccatggttgg tttttataag aggtaataaa attaacattg tacaattaaa
     5821 tgactataaa ttaatgtttt tttttatttt ttttacacag ctaccttcaa aaagggatcc
     5881 atggcagccg agactcggaa cgtggccgga gcagaggccc caccgcccca gaagcgctac
     5941 taccggcaac gtgctcacga taaccccatg gcggaccaca cgctgcgcta ccctgtgaag
     6001 ccagaggaga tggactggtc tgagctatac ccagagttct tcgctccact cactcaaaat
     6061 cagagccacg atgacccaaa ggataagaaa gaaaagagag ctcaggccca agtggagttt
     6121 gcagacatag gctgtggcta tggtggcctg ttagtggaac tgtcaccgct gttcccagac
     6181 acacttattc tgggtctgga gatccgggtg aaggtctcag actatgtaca agaccggatt
     6241 cgggccctac gcgcagctcc tgcaggtggc ttccagaaca tcgcctgtct ccgtagcaat
     6301 gccatgaagc accttcctaa cttcttctac aagggccagc tgacaaagat gttcttcctc
     6361 ttccccgacc cacatttcaa gcggacaaag cacaagtggc gaatcatcag tcccaccctg
     6421 ctagcagaat atgcctacgt gctaagagtt ggggggctgg tgtataccat aaccgatgtg
     6481 ctggagctac acgactggat gtgcactcat ttcgaagagc acccactgtt tgagcgtgtg
     6541 cctctggagg acctgagtga agaccccgtt gtgggacatc taggcacctc aactgaggag
     6601 gggaagaaag ttctacgtaa tggagggaag aatttcccag ccatcttccg aagaatacaa
     6661 gatcccgtcc tccaggcagt gacctcccaa accagcctgc ctggtcactg aatcgatgat
     6721 gatccagaca tgataagata cattgatgag tttggacaaa ccacaactag aatgcagtga
     6781 aaaaaatgct ttatttgtga aatttgtgat gctattgctt tatttgtaac cattataagc
     6841 tgcaataaac aagttaacaa caacaattgc attcatttta tgtttcaggt tcagggggag
     6901 gtgtgggagg ttttttaaag caagtaaaac ctctacaaat gtggtatggc tgattatgat
     6961 cctctagatc agatctcttg tttattgcag cttataatgg ttacaaataa agcaatagca
     7021 tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac
     7081 tcatcaatgt atcttatcat gtctggatct acgtaatacg actcactata gttctagagg
     7141 ctcgagaggg gccgctttac ttgtacagct cgtccatgcc gagagtgatc ccggcggcgg
     7201 tcacgaactc cagcaggacc atgtgatcgc gcttctcgtt ggggtctttg ctcagggcgg
     7261 actgggtgct caggtagtgg ttgtcgggca gcagcacggg gccgtcgccg atgggggtgt
     7321 tctgctggta gtggtcggcg agctgcacgc tgccgtcctc gatgttgtgg cggatcttga
     7381 agttcacctt gatgccgttc ttctgcttgt cggccatgat atagacgttg tggctgttgt
     7441 agttgtactc cagcttgtgc cccaggatgt tgccgtcctc cttgaagtcg atgcccttca
     7501 gctcgatgcg gttcaccagg gtgtcgccct cgaacttcac ctcggcgcgg gtcttgtagt
     7561 tgccgtcgtc cttgaagaag atggtgcgct cctggacgta gccttcgggc atggcggact
     7621 tgaagaagtc gtgctgcttc atgtggtcgg ggtagcggct gaagcactgc acgccgtagg
     7681 tcagggtggt cacgagggtg ggccagggca cgggcagctt gccggtggtg cagatgaact
     7741 tcagggtcag cttgccgtag gtggcatcgc cctcgccctc gccggacacg ctgaacttgt
     7801 ggccgtttac gtcgccgtcc agctcgacca ggatgggcac caccccggtg aacagctctt
     7861 cgcccttgct caccatggtc actgtctgct ttgctgttgg tctgggctcc tgggtcactg
     7921 gcttactaat ggagtcttta tgtatgagga ctcttatcaa ttgttcttct ataaaggtct
     7981 gcagtgtttc tgttcgtccc ctacatggac acccagagcc tcctaaatac aggagccctg
     8041 ataactgcac aagtgctcag attccagcag ggtggaaatg agataaagtg tgcagatggg
     8101 gagggggacg tgaatgagag atttgaggga tgaaaaggat ggatgaacgc attgaaaata
     8161 gcccctttca caaaataata ccagtaaatt gtcatataat taatgaccag acctttacct
     8221 gtaaatgatg tgatgttcat atacagaaga atgccaacaa tttacagatg attttacaaa
     8281 ttcttaattt tccttttcta aatggatttg ttcacacatt atctcttcaa agcaattgaa
     8341 ttttctggaa aagattggct gtgtgtgtaa aaggtgctac tgaggaattt aatgtgacat
     8401 ggaatgaagc aaacagcagc ctaagacagg atggcaggaa aaatgtcaca tgtttaaaat
     8461 aagagtgaaa ccaacgacct ggacttatag agactgtgtg tccttaggca aagctcttgt
     8521 acaaacagtg cttgtgactg tgaaattcgg ggtttgcctg gattgtgtta aatgtgtgct
     8581 tggatgtatc ataaaaaaga atcggttctc tgtatttgac attttctgta atttatctaa
     8641 agttacaact ggctatgcct gatttttatc actattgaat caatcatttt aaagaatgtt
     8701 aagtctgctg atttggagag ctcatttaca tttattttga atgtctctta tttaacacaa
     8761 ctgatttaag ctttagatct gcgaagatac ggccacgggt gctcttgatc ctgtggctga
     8821 ttttggactg tgctgctcgc agctgctgat gaatcacata cttcctccat tttcttccac
     8881 tgattgactg ttataatttc cctaatttcc aggtcaaggt gctgtgcatt gtggtaatag
     8941 atgtgacatg attgcattca ttttatgttt caggttcagg gggaggtgtg ggaggttttt
     9001 taaagcaagt aaaacctcta caaatgtggt atggctgatt atgatcctct agatcagatc
     9061 cactagttct agagcggcca ttaccctgtt atccctaggc cgccaccgcg gtggagctcc
     9121 aattcgccct atagtgagtc gtattacgcg cgctcactgg ccgtcgtttt acaacgtcgt
     9181 gactgggaaa accctggcgt tacccaactt aatcgccttg cagcacatcc ccctttcgcc
     9241 agctggcgta atagcgaaga ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg
     9301 aatggcgaat ggaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt
     9361 aaatcagctc attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag
     9421 aatagaccga gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga
     9481 acgtggactc caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg
     9541 aaccatcacc ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc
     9601 ctaaagggag cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg
     9661 aagggaagaa agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc
     9721 gcgtaaccac cacacccgcc gcgcttaatg cgccgctaca gggcgcgtca ggtggcactt
     9781 ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt
     9841 atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta
     9901 tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg
     9961 tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac
    10021 gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg
    10081 aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc
    10141 gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg
    10201 ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat
    10261 gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg
    10321 gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg
    10381 atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc
    10441 ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt
    10501 cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct
    10561 cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc
    10621 gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca
    10681 cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct
    10741 cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt
    10801 taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga
    10861 ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca
    10921 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac
    10981 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg
    11041 taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag
    11101 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac
    11161 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt
    11221 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
    11281 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc
    11341 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc
    11401 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
    11461 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa
    11521 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt
    11581 tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg
    11641 ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag
    11701 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
    11761 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
    11821 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
    11881 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagcgcg
    11941 caattaaccc tcactaaagg gaacaaaagc tgggtaccgg gccccccctc gaattaccct
    12001 gttatcccta tcgagcaaaa tcaatcctaa tgtaaaatag tatacgttca tcattataat
    12061 gcaaacactg tagaactgtt gtgttgtgcc ttgcatttca ttctcagttt cttatcgtct
    12121 gtctcttttt acgacagagc tagaaaaaac tagccttcat ttgccatcgc cgtatgcaaa
    12181 tccactccag aactcaacag gtttgttctg cggcgggtgt aagtgtgaac accggcatgt
    12241 aagtgcgctt gagcaggaga ataaagcgct cagagacacc ggaggaatcc tttattttgc
    12301 gttgcgcctc aggggattcg ctgagcct
//
