LOCUS       sequence-443476-       13359 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express MDM2 gene in mesenchymal lineage of Zebrafish..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13359)
  AUTHORS   Ali RH, Orellana EA, Lee SH, Chae YC, Chen Y, Clauwaert J, Kennedy 
            AL, Gutierrez AE, Papke DJ, Valenzuela M, Silverman B, Falzetta A, 
            Ficarro SB, Marto JA, Fletcher CDM, Perez-Atayde A, Alcindor T, 
            Shimamura A, Prensner JR, Gregory RI, Gutierrez A
  TITLE     A methyltransferase-independent role for METTL1 in tRNA 
            aminoacylation and oncogenic transformation.
  JOURNAL   Mol Cell. 2025 Mar 6;85(5):948-961.e11. doi: 
            10.1016/j.molcel.2025.01.003. Epub 2025 Jan 31.
  PUBMED    39892392
REFERENCE   2  (bases 1 to 13359)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2025.01.003"; journalName: "Mol Cell"; date: 
            "2025-03-6- 6"; volume: "85"; issue: "5"; pages: "948-961.e11"
COMMENT     Sequence Label: rag2:MDM2_CE_pISceI
FEATURES             Location/Qualifiers
     source          1..13359
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(231..247)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(231..247)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     promoter        complement(302..320)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(330..347)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(330..346)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(339..361)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      complement(487..942)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(624..645)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     836..855
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        969..1073
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1074..1142,1143..1934)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1292..1311)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2105..2693
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2594..2613
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2847..2864
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2981..3002
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(3017..3022,3023..3040,3041..3047)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3055..3071
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     3060..3082
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     3079..3095
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3079..3095
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     3114..3134
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        3116..3134
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     gap             4753..4852
                     /estimated_length=100
     polyA_signal    11245..11379
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             join(12466..12468,12469..12471,12472..13185)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(12466..12468,12469..12471,12472..13182)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(12511..12532)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(12772..12791)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     13119..13140
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc atttttttca
       61 ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggatcatc
      121 atcgattgca ttcattttat gtttcaggtt cagggggagg tgtgggaggt tttttaaagc
      181 aagtaaaacc tctacaaatg tggtatggct gattatgatc ctctagatca gatccactag
      241 ttctagagcg gccattaccc tgttatccct aggccgccac cgcggtggag ctccaattcg
      301 ccctatagtg agtcgtatta cgcgcgctca ctggccgtcg ttttacaacg tcgtgactgg
      361 gaaaaccctg gcgttaccca acttaatcgc cttgcagcac atcccccttt cgccagctgg
      421 cgtaatagcg aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctgaatggc
      481 gaatggaaat tgtaagcgtt aatattttgt taaaattcgc gttaaatttt tgttaaatca
      541 gctcattttt taaccaatag gccgaaatcg gcaaaatccc ttataaatca aaagaataga
      601 ccgagatagg gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg
      661 actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacta cgtgaaccat
      721 caccctaatc aagttttttg gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag
      781 ggagcccccg atttagagct tgacggggaa agccggcgaa cgtggcgaga aaggaaggga
      841 agaaagcgaa aggagcgggc gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa
      901 ccaccacacc cgccgcgctt aatgcgccgc tacagggcgc gtcaggtggc acttttcggg
      961 gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc
     1021 tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta
     1081 ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg
     1141 ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg
     1201 gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac
     1261 gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg
     1321 acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt
     1381 actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg
     1441 ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac
     1501 cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt
     1561 gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag
     1621 caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc
     1681 aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc
     1741 ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta
     1801 tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg
     1861 ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga
     1921 ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac
     1981 ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa
     2041 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
     2101 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
     2161 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
     2221 gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc
     2281 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
     2341 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
     2401 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
     2461 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
     2521 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
     2581 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
     2641 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
     2701 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc
     2761 ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg
     2821 ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc
     2881 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
     2941 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
     3001 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
     3061 gcggataaca atttcacaca ggaaacagct atgaccatga ttacgccaag cgcgcaatta
     3121 accctcacta aagggaacaa aagctgggta ccgggccccc cctcgaatta ccctgttatc
     3181 cctatcgagc aaaatcaatc ctaatgtaaa atagtatacg ttcatcatta taatgcaaac
     3241 actgtagaac tgttgtgttg tgccttgcat ttcattctca gtttcttatc gtctgtctct
     3301 ttttacgaca gagctagaaa aaactagcct tcatttgcca tcgccgtatg caaatccact
     3361 ccagaactca acaggtttgt tctgcggcgg gtgtaagtgt gaacaccggc atgtaagtgc
     3421 gcttgagcag gagaataaag cgctcagaga caccggagga atcctttatt ttgcgttgcg
     3481 cctcagggga ttcgctgagc cttctcttca tggccctgct cttcagacac aggtgctccg
     3541 ctgtttgtgt ttttcactgt tcttcaggca ctcgtggtta cgggtttttg ttttgcactc
     3601 ttgcacacac atctctcggc ctccaaaaca aacacacaac tgcacgaggg ctcgaagccc
     3661 ccaccgaaaa acaaaaacat tatgctaatg aggaaggcac agctgttccc ttcttcactc
     3721 tctctctcgt tctttcaaca acagtcgcct aatttctctc gttcctttaa agagccgcca
     3781 agcagaaaca gttaatgcaa atataagcct gcgggaaata aggagaattt ttacaaaatc
     3841 cgagcttccc ctcctgccaa aatgtgatgt gtgactcacg cattcgatat ttggtgtaat
     3901 gagagtgaat cattctgctc cacaccgaac ccgatgccac agaccactca gatgcacacc
     3961 gaggagacta ttttaacagc acattacatc cagactgtca cagatgcatt accaagagtg
     4021 tgatttcagt gtcgaatgca ggtcttaaag gaaaatctgt tgcgattgca tgtgcgctaa
     4081 ctagacacga ccaacgaatg ggaacaatct gtgaaatatt atacaatcac aggcaattaa
     4141 aagatgttta aagttcaatt tagttttaat taatatgtta tataaaacta atctgtttta
     4201 aatgtatatt tatgatttta gctttagcta gcctcttgac cagcagctga agtgtccggc
     4261 aagtacctaa aagggagacc acatgggaaa gctaggttgc tcccggaaga ggtgttagta
     4321 aaccagcagg tctacctgtg gtctgtgtgg gtcctaatgc cccagtataa accagcaggt
     4381 caacctgtgg tctgtgtggg tcctaatgcc ccagtataaa ccagcaggtc tacctgtggt
     4441 ctgtgtgggt cctaatgccc cagtataaac cagcaggtca acctgtggtc tgtgtgggtc
     4501 ctaatgcccc agtataaacc agcaggtcta cctgtggtct gtgtgggtcc taatgcccca
     4561 gtataaacca gcaggtcaac ctgtggtctg tgtgggtcct aatgccccag tataaaccag
     4621 caggtctacc tgtggtctgt gtgggtccta atgccccagt ataaaccagc aggtcaacct
     4681 gtggtctgtg tgggtcctaa tgccccagta taaaccagca ggtctacctg tggtctgtgt
     4741 gggtcctaat gcnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnctacctgt
     4861 ggtctgtgtg ggtcctaatg ccccagtata aaccagcagg tcaacctgtg gtctgtgtgg
     4921 gtcctaatgc cccagtataa accagcaggt ctacctgtgg tctgtgtggg tcctaatgca
     4981 ccagtataaa ccagcaggtc aacctgtggt ctgtgtgggt tctaatgccc cagtataaac
     5041 cagcaggtca acctgtggtc tgtgtgggtc caaatgcccc agtacaaacc agcaggtcaa
     5101 cctgtggtct gtgtgggtcc taatgcccca gtataaacca gcaggtcaac ctgtggtctg
     5161 tgtgggtcct aatgccccgg tacaaaccag catgtcaacc tgtggtctgt gtgggtccta
     5221 atgccccggt ataaaccagc agatcaacct gtggtctgtg tgggtcctaa tgccccagta
     5281 taaaccagca gatcaacctg tggtctgtgt gggtcctaaa gtcccaggat aaaccagcat
     5341 gtcaacctgt ggtctgtgtg ggtcctaatg ccccggtata aaccagcaga tcaacctgtg
     5401 gtctgtgtgg gtcctaatgc cccaggataa accagcaggt caacctgtgg tctgtgtggg
     5461 tcctaatgcc ccagtataaa ccagcaggtc aacctgtggt ctgtgtgggt cctaatgccc
     5521 cagtatattg atggagactc tatactgctc ggtgagctcc atctttcgga taagatgtta
     5581 aaacaaggtc ccgactctct gtggtggtaa aaaattccca ggatgtcctt cctaccctgg
     5641 catcctggac aaatttgctc actgtcctct gtccatcatg gcctcctaac catccccata
     5701 tcataattgg ctttatcact ctagctgggg ccgaaatcgc atacttccat tttatgaggt
     5761 acgctaaaat cagtatgcga gccaagtaat atgtccgaat acttccggcg agattctaga
     5821 gtgtgcattc gatgcacgct acgctattcc atgatgcccc gtgagagaat tcttgaatcg
     5881 gagagaagca acgcaactca tgcgggtagg ttacgtgacg atgacatatt ggcggatgta
     5941 gtacgtccaa gttccattca tactgctcac attcatattt tatagaatgt tcttttccca
     6001 cagccaagta gtaagtttaa attcaaatac agtacctact aagtagtagt agacggtttc
     6061 ggacgcagcc cctgtctcct ctccaccaat atactggtgt gtagtgtgcg gtctggtgca
     6121 atatggctgc catcgcgtca tccaggtgga tgctgcacac tggtggttga tgaggagatt
     6181 ttgcccaaaa aagtgttttg agtttccaga aaagcgcgat ataaatgtaa ggaattatca
     6241 ttagcttatt ataatggaac tttctggata acttgtgcca gagctagggg ccattaaaac
     6301 tttgccctgg ggtacctcag ggagctgcaa ggaagtgcta tagggctaat gaaatgtttg
     6361 ggggaaaaaa aaaaatttta taagtaaaaa taaaactaaa ataattaata atttcattgt
     6421 tgaaaattat agtaaaattg ataaacaaga taagcagcat tagtataaaa aactaaaaac
     6481 aaacgtaatt taaaataatt aaattgtttt aaataaatta tacaaaataa tgtttacaac
     6541 gacacggtgg tgcagtaggt agtgctgtcg ccagcaaagc aagaaggtcg ctggttcgag
     6601 cctcagctgg atcacttgat gtttctgtgt ggagcattcg cgtgcgttta accccagatt
     6661 aataaagaga ctaagccgaa aagaaaataa atgtatgaaa atgtttacgt aaaatgtaaa
     6721 taaatgcatg attaataaaa tatatgaatt ttaaagattg aggtagaatt gttttattat
     6781 tcacacattt ggttattttt aatcagtgac aacttgtgat gctctttata ttgtttatta
     6841 cgctactttg aatattcagt ctcaaattgc atgtaaatac gacttcaaag cagaattagt
     6901 ttaatttaat tgcctgtaaa cagtgttgta ctttgatggt gattgtctga taataatatg
     6961 atttcactat ttaaaatttg aaaaaaaaaa atctgaaaaa tactcaccgg ccactttatt
     7021 aggtacacct gtctaactgc ttgttaacgt aaatttctaa tcagccaatc accaggcagc
     7081 aactcagtgc atttaggcat gtagacatgg tcaagacgat ctgctgcagt tcaaagcgag
     7141 catcagaatg gagaagcaag gtgatttaag tgactttaaa cgtgacgtgt ttgttggtgc
     7201 cagacgggct ggtctgagta tttcagaaac tgctgattta ctggggtttt cacacacaaa
     7261 catctctggg gtttacagag aatggtccaa aaaaagataa tatccagtga gcggcagttc
     7321 tgtgggggca aatgtcttgt tgatgccaga ggtcagagga gaatggcctg actggttcca
     7381 gctgatagaa aggcaacagt aattcaaata accactcgtt agaactgagg catgcagaag
     7441 agcacctttg aacacaacat gtcgaacctt gaggcgggtg ggctacagca gctcaagacc
     7501 acatcgggtg ccactcctgt cagctaagaa caggaaactg aggctacaat tcgcacaggc
     7561 tcaacataag tagacaatag aagattgaac attatgttgc ctggtcagat ggatacattc
     7621 taccttgtat caacagttca ggctggtgat ggtggtgtaa tggtgtgggg gatatattct
     7681 tggcacactt tgggcccatt agtaccaatt aaccatcgtg tcaacgccac agcctaactg
     7741 agtattgttg ctgaccatgt tcatttcttt ttgaccacag tgtacccatc ttctgatggc
     7801 tacttcctgc aggataatgc accatgtcat aaagcgtgaa tgatctcaga ctggtttctt
     7861 gaacatgaca atgagttcac tgtgctcgaa tgacctccac agtcaccaga tctcaatcca
     7921 atagagcgcc tttggggtgt ggtagaatgg aagattcgca tcctggatgt gcagccgaca
     7981 aatctgcatg atgctagcat gtcaatatgg accaaaatct ctgaggaatt ttccagtacc
     8041 tggttgaaat gcaaaaggaa gggaatctat gctaaggcaa aaagaggtcc aacccagtac
     8101 tagtggggtg tacataataa agtggccggt gagtgtatat tgttaaggat atctagataa
     8161 tccaaataat ttacagcact atttaaaaaa aaaactttat gtcaccaagt ctgcatttat
     8221 ttgatcacaa ttacagtcga aaaagcaata tcgggataca ttgtttccat ttaaaataac
     8281 tatattatat tttaaatatt tttgacaatg ttatttcttt aatgccaaag ctaaattgta
     8341 ttcaatctga attttagtgt cacatgatcc ttcagaaaat attaacacat tgctaaaaat
     8401 gtatattatt atcaatttgg attttttttt ttttttgtat attaacctat gctattggtt
     8461 actatacaaa agtattactg tgaatatatg catatacaga accgtatcaa gcgcgggggg
     8521 tggttgggtt atgtccccat ttggaaagac ctggaaacat tcataatgac taaaattgca
     8581 cggaaaaata taatatctat gagtattgct gatcagcagc acaaaaccag aactccatat
     8641 taataaacag ccaatgatgc agtgagtttg tgcatgtttt cattcaaact cgtgtgattg
     8701 tgctcaaaca cctgttgtga gcagcagttg caaacagtaa acctcaagag gagccagtca
     8761 accttcacaa acactcaacc tctggctcac tcactaaaca attcgcgtta atatctaaaa
     8821 taaaaatttc cagcaccaaa cagctctcag atttcggagt acactgacca agaggtaaga
     8881 agtaacgtta cattactagg acgactattg taagactaga tatgtaatat ttggaagcgt
     8941 acgtgtttta agtgacatat agagtagagg aactgatgga aatacgattt gcataatgta
     9001 aaatgatata tattaaaata taggctacag taggtaacgt ggtttacata taacgttact
     9061 gtgttaatgt agtgctcagt tactcgtgtt gacagttatt attagtgctt gctaatttgt
     9121 ttgttaatgc ttctcttgcg tatttgatta ccttcaggcg aatctaaaac atatcagatg
     9181 tagaaacggc agaaaaatgt tgaagaaaat acgagaatag tagcttttgg agcacataga
     9241 aaagtacatc agataaagcc taccgtttaa acgcagacgc cgctctcgca ctgaatgctg
     9301 ggtactgtag tccttatatt gtatgcttaa tgtgcgtctc aacgggaaac tagagttagg
     9361 aaaactacaa tttcctgtgg aaaatgtttc tgttttcggg gaaacagcga aagaaagata
     9421 tggttccgcg tttttttctt actttgaata ataaaataag acctaactac acacttcgct
     9481 tactcttatg ctaatttgaa gcactagcat tctttgatac aatgataatc atttaaacat
     9541 cacagttata ctacaaatac tatggttaaa ctatgtctag tgtagtgtaa cactatggtt
     9601 gccattgtta tctttaagtt taagtattat ctgtggtaaa gccatggttg gtttttataa
     9661 gaggtaataa aattaacatt gtacaattaa atgactataa attaatgttt ttttttattt
     9721 tttttacaca gctaccttca aaaagggatc cgccaccatg tgcaatacca acatgtctgt
     9781 acctactgat ggtgctgtaa ccacctcaca gattccagct tcggaacaag agaccctggt
     9841 tagaccaaag ccattgcttt tgaagttatt aaagtctgtt ggtgcacaaa aagacactta
     9901 tactatgaaa gaggttcttt tttatcttgg ccagtatatt atgactaaac gattatatga
     9961 tgagaagcaa caacatattg tatattgttc aaatgatctt ctaggagatt tgtttggcgt
    10021 gccaagcttc tctgtgaaag agcacaggaa aatatatacc atgatctaca ggaacttggt
    10081 agtagtcaat cagcaggaat catcggactc aggtacatct gtgagtgaga acaggtgtca
    10141 ccttgaaggt gggagtgatc aaaaggacct tgtacaagag cttcaggaag agaaaccttc
    10201 atcttcacat ttggtttcta gaccatctac ctcatctaga aggagagcaa ttagtgagac
    10261 agaagaaaat tcagatgaat tatctggtga acgacaaaga aaacgccaca aatctgatag
    10321 tatttccctt tcctttgatg aaagcctggc tctgtgtgta ataagggaga tatgttgtga
    10381 aagaagcagt agcagtgaat ctacagggac gccatcgaat ccggatcttg atgctggtgt
    10441 aagtgaacat tcaggtgatt ggttggatca ggattcagtt tcagatcagt ttagtgtaga
    10501 atttgaagtt gaatctctcg actcagaaga ttatagcctt agtgaagaag gacaagaact
    10561 ctcagatgaa gatgatgagg tatatcaagt tactgtgtat caggcagggg agagtgatac
    10621 agattcattt gaagaagatc ctgaaatttc cttagctgac tattggaaat gcacttcatg
    10681 caatgaaatg aatccccccc ttccatcaca ttgcaacaga tgttgggccc ttcgtgagaa
    10741 ttggcttcct gaagataaag ggaaagataa aggggaaatc tctgagaaag ccaaactgga
    10801 aaactcaaca caagctgaag agggctttga tgttcctgat tgtaaaaaaa ctatagtgaa
    10861 tgattccaga gagtcatgtg ttgaggaaaa tgatgataaa attacacaag cttcacaatc
    10921 acaagaaagt gaagactatt ctcagccatc aacttctagt agcattattt atagcagcca
    10981 agaagatgtg aaagagtttg aaagggaaga aacccaagac aaagaagaga gtgtggaatc
    11041 tagtttgccc cttaatgcca ttgaaccttg tgtgatttgt caaggtcgac ctaaaaatgg
    11101 ttgcattgtc catggcaaaa caggacatct tatggcctgc tttacatgtg caaagaagct
    11161 aaagaaaagg aataagccct gcccagtatg tagacaacca attcaaatga ttgtgctaac
    11221 ttatttcccc taggaatcga tgatgatcca gacatgataa gatacattga tgagtttgga
    11281 caaaccacaa ctagaatgca gtgaaaaaaa tgctttattt gtgaaatttg tgatgctatt
    11341 gctttatttg taaccattat aagctgcaat aaacaagtta acaacaacaa tcatgtcaca
    11401 tctattacca caatgcacag caccttgacc tggaaattag ggaaattata acagtcaatc
    11461 agtggaagaa aatggaggaa gtatgtgatt catcagcagc tgcgagcagc acagtccaaa
    11521 atcagccaca ggatcaagag cacccgtggc cgtatcttcg cagatctaaa gcttaaatca
    11581 gttgtgttaa ataagagaca ttcaaaataa atgtaaatga gctctccaaa tcagcagact
    11641 taacattctt taaaatgatt gattcaatag tgataaaaat caggcatagc cagttgtaac
    11701 tttagataaa ttacagaaaa tgtcaaatac agagaaccga ttctttttta tgatacatcc
    11761 aagcacacat ttaacacaat ccaggcaaac cccgaatttc acagtcacaa gcactgtttg
    11821 tacaagagct ttgcctaagg acacacagtc tctataagtc caggtcgttg gtttcactct
    11881 tattttaaac atgtgacatt tttcctgcca tcctgtctta ggctgctgtt tgcttcattc
    11941 catgtcacat taaattcctc agtagcacct tttacacaca cagccaatct tttccagaaa
    12001 attcaattgc tttgaagaga taatgtgtga acaaatccat ttagaaaagg aaaattaaga
    12061 atttgtaaaa tcatctgtaa attgttggca ttcttctgta tatgaacatc acatcattta
    12121 caggtaaagg tctggtcatt aattatatga caatttactg gtattatttt gtgaaagggg
    12181 ctattttcaa tgcgttcatc catccttttc atccctcaaa tctctcattc acgtccccct
    12241 ccccatctgc acactttatc tcatttccac cctgctggaa tctgagcact tgtgcagtta
    12301 tcagggctcc tgtatttagg aggctctggg tgtccatgta ggggacgaac agaaacactg
    12361 cagaccttta tagaagaaca attgataaga gtcctcatac ataaagactc cattagtaag
    12421 ccagtgaccc aggagcccag accaacagca aagcagacag tgaccatggt gagcaagggc
    12481 gaagagctgt tcaccggggt ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc
    12541 cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa gctgaccctg
    12601 aagttcatct gcaccaccgg caagctgccc gtgccctggc ccaccctcgt gaccaccctg
    12661 acctacggcg tgcagtgctt cagccgctac cccgaccaca tgaagcagca cgacttcttc
    12721 aagtccgcca tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa ggacgacggc
    12781 aactacaaga cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag
    12841 ctgaagggca tcgacttcaa ggaggacggc aacatcctgg ggcacaagct ggagtacaac
    12901 tacaacagcc acaacgtcta tatcatggcc gacaagcaga agaacggcat caaggtgaac
    12961 ttcaagatcc gccacaacat cgaggacggc agcgtgcagc tcgccgacca ctaccagcag
    13021 aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca accactacct gagcacccag
    13081 tccgccctga gcaaagaccc caacgagaag cgcgatcaca tggtcctgct ggagttcgtg
    13141 accgccgccg ggatcactct cggcatggac gagctgtaca agtaaagcgg cccctctcga
    13201 gcctctagaa ctatagtgag tcgtattacg tagatccaga catgataaga tacattgatg
    13261 agtttggaca aaccacaact agaatgcagt gaaaaaaatg ctttatttgt gaaatttgtg
    13321 atgctattgc tttatttgta accattataa gctgcaata
//
