LOCUS       sequence-443479-       11546 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express TSPAN31 gene in mesenchymal lineage of Zebrafish..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11546)
  AUTHORS   Ali RH, Orellana EA, Lee SH, Chae YC, Chen Y, Clauwaert J, Kennedy 
            AL, Gutierrez AE, Papke DJ, Valenzuela M, Silverman B, Falzetta A, 
            Ficarro SB, Marto JA, Fletcher CDM, Perez-Atayde A, Alcindor T, 
            Shimamura A, Prensner JR, Gregory RI, Gutierrez A
  TITLE     A methyltransferase-independent role for METTL1 in tRNA 
            aminoacylation and oncogenic transformation.
  JOURNAL   Mol Cell. 2025 Mar 6;85(5):948-961.e11. doi: 
            10.1016/j.molcel.2025.01.003. Epub 2025 Jan 31.
  PUBMED    39892392
REFERENCE   2  (bases 1 to 11546)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2025.01.003"; journalName: "Mol Cell"; date: 
            "2025-03-6- 6"; volume: "85"; issue: "5"; pages: "948-961.e11"
COMMENT     Sequence Label: rag2:TSPAN31_CE_pISceI
FEATURES             Location/Qualifiers
     source          1..11546
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    5719..5853
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     polyA_signal    5874..6008
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(join(6310..7023,7024..7026,7027..7029))
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             complement(join(6313..7023,7024..7026,7027..7029))
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(6355..6376)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     6704..6723
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     6963..6984
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(8210..8226)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8210..8226)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     promoter        complement(8281..8299)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8309..8326)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8309..8325)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8318..8340)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      complement(8466..8921)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8603..8624)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     8815..8834
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        8948..9052
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9053..9121,9122..9913)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9271..9290)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10084..10672
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10573..10592
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10826..10843
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    10960..10981
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(10996..11001,11002..11019,11020..11026)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11034..11050
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     11039..11061
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     11058..11074
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     11058..11074
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     11093..11113
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        11095..11113
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 gcactcgtgg ttacgggttt ttgttttgca ctcttgcaca cacatctctc ggcctccaaa
       61 acaaacacac aactgcacga gggctcgaag cccccaccga aaaacaaaaa cattatgcta
      121 atgaggaagg cacagctgtt cccttcttca ctctctctct cgttctttca acaacagtcg
      181 cctaatttct ctcgttcctt taaagagccg ccaagcagaa acagttaatg caaatataag
      241 cctgcgggaa ataaggagaa tttttacaaa atccgagctt cccctcctgc caaaatgtga
      301 tgtgtgactc acgcattcga tatttggtgt aatgagagtg aatcattctg ctccacaccg
      361 aacccgatgc cacagaccac tcagatgcac accgaggaga ctattttaac agcacattac
      421 atccagactg tcacagatgc attaccaaga gtgtgatttc agtgtcgaat gcaggtctta
      481 aaggaaaatc tgttgcgatt gcatgtgcgc taactagaca cgaccaacga atgggaacaa
      541 tctgtgaaat attatacaat cacaggcaat taaaagatgt ttaaagttca atttagtttt
      601 aattaatatg ttatataaaa ctaatctgtt ttaaatgtat atttatgatt ttagctttag
      661 ctagcctctt gaccagcagc tgaagtgtcc ggcaagtacc taaaagggag accacatggg
      721 aaagctaggt tgctcccgga agaggtgtta gtaaaccagc aggtctacct gtggtctgtg
      781 tgggtcctaa tgccccagta taaaccagca ggtcaacctg tggtctgtgt gggtcctaat
      841 gccccagtat attgatggag actctatact gctcggtgag ctccatcttt cggataagat
      901 gttaaaacaa ggtcccgact ctctgtggtg gtaaaaaatt cccaggatgt ccttcctacc
      961 ctggcatcct ggacaaattt gctcactgtc ctctgtccat catggcctcc taaccatccc
     1021 catatcataa ttggctttat cactctagct ggggccgaaa tcgcatactt ccattttatg
     1081 aggtacgcta aaatcagtat gcgagccaag taatatgtcc gaatacttcc ggcgagattc
     1141 tagagtgtgc attcgatgca cgctacgcta ttccatgatg ccccgtgaga gaattcttga
     1201 atcggagaga agcaacgcaa ctcatgcggg taggttacgt gacgatgaca tattggcgga
     1261 tgtagtacgt ccaagttcca ttcatactgc tcacattcat attttataga atgttctttt
     1321 cccacagcca agtagtaagt ttaaattcaa atacagtacc tactaagtag tagtagacgg
     1381 tttcggacgc agcccctgtc tcctctccac caatatactg gtgtgtagtg tgcggtctgg
     1441 tgcaatatgg ctgccatcgc gtcatccagg tggatgctgc acactggtgg ttgatgagga
     1501 gattttgccc aaaaaagtgt tttgagtttc cagaaaagcg cgatataaat gtaaggaatt
     1561 atcattagct tattataatg gaactttctg gataacttgt gccagagcta ggggccatta
     1621 aaactttgcc ctggggtacc tcagggagct gcaaggaagt gctatagggc taatgaaatg
     1681 tttgggggaa aaaaaaaaat tttataagta aaaataaaac taaaataatt aataatttca
     1741 ttgttgaaaa ttatagtaaa attgataaac aagataagca gcattagtat aaaaaactaa
     1801 aaacaaacgt aatttaaaat aattaaattg ttttaaataa attatacaaa ataatgttta
     1861 caacgacacg gtggtgcagt aggtagtgct gtcgccagca aagcaagaag gtcgctggtt
     1921 cgagcctcag ctggatcact tgatgtttct gtgtggagca ttcgcgtgcg tttaacccca
     1981 gattaataaa gagactaagc cgaaaagaaa ataaatgtat gaaaatgttt acgtaaaatg
     2041 taaataaatg catgattaat aaaatatatg aattttaaag attgaggtag aattgtttta
     2101 ttattcacac atttggttat ttttaatcag tgacaacttg tgatgctctt tatattgttt
     2161 attacgctac tttgaatatt cagtctcaaa ttgcatgtaa atacgacttc aaagcagaat
     2221 tagtttaatt taattgcctg taaacagtgt tgtactttga tggtgattgt ctgataataa
     2281 tatgatttca ctatttaaaa tttgaaaaaa aaaaatctga aaaatactca ccggccactt
     2341 tattaggtac acctgtctaa ctgcttgtta acgtaaattt ctaatcagcc aatcaccagg
     2401 cagcaactca gtgcatttag gcatgtagac atggtcaaga cgatctgctg cagttcaaag
     2461 cgagcatcag aatggagaag caaggtgatt taagtgactt taaacgtgac gtgtttgttg
     2521 gtgccagacg ggctggtctg agtatttcag aaactgctga tttactgggg ttttcacaca
     2581 caaacatctc tggggtttac agagaatggt ccaaaaaaag ataatatcca gtgagcggca
     2641 gttctgtggg ggcaaatgtc ttgttgatgc cagaggtcag aggagaatgg cctgactggt
     2701 tccagctgat agaaaggcaa cagtaattca aataaccact cgttagaact gaggcatgca
     2761 gaagagcacc tttgaacaca acatgtcgaa ccttgaggcg ggtgggctac agcagctcaa
     2821 gaccacatcg ggtgccactc ctgtcagcta agaacaggaa actgaggcta caattcgcac
     2881 aggctcaaca taagtagaca atagaagatt gaacattatg ttgcctggtc agatggatac
     2941 attctacctt gtatcaacag ttcaggctgg tgatggtggt gtaatggtgt gggggatata
     3001 ttcttggcac actttgggcc cattagtacc aattaaccat cgtgtcaacg ccacagccta
     3061 actgagtatt gttgctgacc atgttcattt ctttttgacc acagtgtacc catcttctga
     3121 tggctacttc ctgcaggata atgcaccatg tcataaagcg tgaatgatct cagactggtt
     3181 tcttgaacat gacaatgagt tcactgtgct cgaatgacct ccacagtcac cagatctcaa
     3241 tccaatagag cgcctttggg gtgtggtaga atggaagatt cgcatcctgg atgtgcagcc
     3301 gacaaatctg catgatgcta gcatgtcaat atggaccaaa atctctgagg aattttccag
     3361 tacctggttg aaatgcaaaa ggaagggaat ctatgctaag gcaaaaagag gtccaaccca
     3421 gtactagtgg ggtgtacata ataaagtggc cggtgagtgt atattgttaa ggatatctag
     3481 ataatccaaa taatttacag cactatttaa aaaaaaaact ttatgtcacc aagtctgcat
     3541 ttatttgatc acaattacag tcgaaaaagc aatatcggga tacattgttt ccatttaaaa
     3601 taactatatt atattttaaa tatttttgac aatgttattt ctttaatgcc aaagctaaat
     3661 tgtattcaat ctgaatttta gtgtcacatg atccttcaga aaatattaac acattgctaa
     3721 aaatgtatat tattatcaat ttggattttt tttttttttt tgtatattaa cctatgctat
     3781 tggttactat acaaaagtat tactgtgaat atatgcatat acagaaccgt atcaagcgcg
     3841 gggggtggtt gggttatgtc cccatttgga aagacctgga aacattcata atgactaaaa
     3901 ttgcacggaa aaatataata tctatgagta ttgctgatca gcagcacaaa accagaactc
     3961 catattaata aacagccaat gatgcagtga gtttgtgcat gttttcattc aaactcgtgt
     4021 gattgtgctc aaacacctgt tgtgagcagc agttgcaaac agtaaacctc aagaggagcc
     4081 agtcaacctt cacaaacact caacctctgg ctcactcact aaacaattcg cgttaatatc
     4141 taaaataaaa atttccagca ccaaacagct ctcagatttc ggagtacact gaccaagagg
     4201 taagaagtaa cgttacatta ctaggacgac tattgtaaga ctagatatgt aatatttgga
     4261 agcgtacgtg ttttaagtga catatagagt agaggaactg atggaaatac gatttgcata
     4321 atgtaaaatg atatatatta aaatataggc tacagtaggt aacgtggttt acatataacg
     4381 ttactgtgtt aatgtagtgc tcagttactc gtgttgacag ttattattag tgcttgctaa
     4441 tttgtttgtt aatgcttctc ttgcgtattt gattaccttc aggcgaatct aaaacatatc
     4501 agatgtagaa acggcagaaa aatgttgaag aaaatacgag aatagtagct tttggagcac
     4561 atagaaaagt acatcagata aagcctaccg tttaaacgca gacgccgctc tcgcactgaa
     4621 tgctgggtac tgtagtcctt atattgtatg cttaatgtgc gtctcaacgg gaaactagag
     4681 ttaggaaaac tacaatttcc tgtggaaaat gtttctgttt tcggggaaac agcgaaagaa
     4741 agatatggtt ccgcgttttt ttcttacttt gaataataaa ataagaccta actacacact
     4801 tcgcttactc ttatgctaat ttgaagcact agcattcttt gatacaatga taatcattta
     4861 aacatcacag ttatactaca aatactatgg ttaaactatg tctagtgtag tgtaacacta
     4921 tggttgccat tgttatcttt aagtttaagt attatctgtg gtaaagccat ggttggtttt
     4981 tataagaggt aataaaatta acattgtaca attaaatgac tataaattaa tgtttttttt
     5041 tatttttttt acacagctac cttcaaaaag ggatccatgg tgtgtggcgg tttcgcatgt
     5101 tcaaagaacg ctctctgtgc ccttaatgtc gtttatatgc tcgtttcact gctgttgatc
     5161 ggtgttgcag catggggaaa agggctcgga cttgtctctt caatacatat aattggcggt
     5221 gtgatcgccg tcggcgtatt cttattactg atcgccgtag ccggccttgt aggagccgtg
     5281 aatcatcatc aggtattgct tttcttctat atgattattt tggggctggt gtttatattt
     5341 cagttcgtga tatcctgttc ctgcttggcg atcaataggt ctaagcaaac cgacgtgata
     5401 aacgcatcct ggtgggtaat gagtaataaa acacgcgacg agcttgagcg ttccttcgac
     5461 tgctgcgggc tgtttaatct gactacactc taccagcagg actacgactt ttgtaccgct
     5521 atttgtaaat cccaatctcc tacttgtcaa atgtgcggcg agaaattcct gaaacactct
     5581 gatgaggctc ttaagatatt gggtggcgtc ggtcttttct tctctttcac cgaaattctg
     5641 ggtgtatggc ttgctatgag gttccgtaac cagaaagacc cccgtgcaaa tccaagcgct
     5701 ttcctgtaaa tcgatgatga tccagacatg ataagataca ttgatgagtt tggacaaacc
     5761 acaactagaa tgcagtgaaa aaaatgcttt atttgtgaaa tttgtgatgc tattgcttta
     5821 tttgtaacca ttataagctg caataaacaa gttaacaaca acaattcgat gatgatccag
     5881 acatgataag atacattgat gagtttggac aaaccacaac tagaatgcag tgaaaaaaat
     5941 gctttatttg tgaaatttgt gatgctattg ctttatttgt aaccattata agctgcaata
     6001 aacaagttaa caacaacaat tgcattcatt ttatgtttca ggttcagggg gaggtgtggg
     6061 aggtttttta aagcaagtaa aacctctaca aatgtggtat ggctgattat gatcctctag
     6121 atcagatctc ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa
     6181 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa
     6241 tgtatcttat catgtctgga tctacgtaat acgactcact atagttctag aggctcgaga
     6301 ggggccgctt tacttgtaca gctcgtccat gccgagagtg atcccggcgg cggtcacgaa
     6361 ctccagcagg accatgtgat cgcgcttctc gttggggtct ttgctcaggg cggactgggt
     6421 gctcaggtag tggttgtcgg gcagcagcac ggggccgtcg ccgatggggg tgttctgctg
     6481 gtagtggtcg gcgagctgca cgctgccgtc ctcgatgttg tggcggatct tgaagttcac
     6541 cttgatgccg ttcttctgct tgtcggccat gatatagacg ttgtggctgt tgtagttgta
     6601 ctccagcttg tgccccagga tgttgccgtc ctccttgaag tcgatgccct tcagctcgat
     6661 gcggttcacc agggtgtcgc cctcgaactt cacctcggcg cgggtcttgt agttgccgtc
     6721 gtccttgaag aagatggtgc gctcctggac gtagccttcg ggcatggcgg acttgaagaa
     6781 gtcgtgctgc ttcatgtggt cggggtagcg gctgaagcac tgcacgccgt aggtcagggt
     6841 ggtcacgagg gtgggccagg gcacgggcag cttgccggtg gtgcagatga acttcagggt
     6901 cagcttgccg taggtggcat cgccctcgcc ctcgccggac acgctgaact tgtggccgtt
     6961 tacgtcgccg tccagctcga ccaggatggg caccaccccg gtgaacagct cttcgccctt
     7021 gctcaccatg gtcactgtct gctttgctgt tggtctgggc tcctgggtca ctggcttact
     7081 aatggagtct ttatgtatga ggactcttat caattgttct tctataaagg tctgcagtgt
     7141 ttctgttcgt cccctacatg gacacccaga gcctcctaaa tacaggagcc ctgataactg
     7201 cacaagtgct cagattccag cagggtggaa atgagataaa gtgtgcagat ggggaggggg
     7261 acgtgaatga gagatttgag ggatgaaaag gatggatgaa cgcattgaaa atagcccctt
     7321 tcacaaaata ataccagtaa attgtcatat aattaatgac cagaccttta cctgtaaatg
     7381 atgtgatgtt catatacaga agaatgccaa caatttacag atgattttac aaattcttaa
     7441 ttttcctttt ctaaatggat ttgttcacac attatctctt caaagcaatt gaattttctg
     7501 gaaaagattg gctgtgtgtg taaaaggtgc tactgaggaa tttaatgtga catggaatga
     7561 agcaaacagc agcctaagac aggatggcag gaaaaatgtc acatgtttaa aataagagtg
     7621 aaaccaacga cctggactta tagagactgt gtgtccttag gcaaagctct tgtacaaaca
     7681 gtgcttgtga ctgtgaaatt cggggtttgc ctggattgtg ttaaatgtgt gcttggatgt
     7741 atcataaaaa agaatcggtt ctctgtattt gacattttct gtaatttatc taaagttaca
     7801 actggctatg cctgattttt atcactattg aatcaatcat tttaaagaat gttaagtctg
     7861 ctgatttgga gagctcattt acatttattt tgaatgtctc ttatttaaca caactgattt
     7921 aagctttaga tctgcgaaga tacggccacg ggtgctcttg atcctgtggc tgattttgga
     7981 ctgtgctgct cgcagctgct gatgaatcac atacttcctc cattttcttc cactgattga
     8041 ctgttataat ttccctaatt tccaggtcaa ggtgctgtgc attgtggtaa tagatgtgac
     8101 atgattgcat tcattttatg tttcaggttc agggggaggt gtgggaggtt ttttaaagca
     8161 agtaaaacct ctacaaatgt ggtatggctg attatgatcc tctagatcag atccactagt
     8221 tctagagcgg ccattaccct gttatcccta ggccgccacc gcggtggagc tccaattcgc
     8281 cctatagtga gtcgtattac gcgcgctcac tggccgtcgt tttacaacgt cgtgactggg
     8341 aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca tccccctttc gccagctggc
     8401 gtaatagcga agaggcccgc accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg
     8461 aatggaaatt gtaagcgtta atattttgtt aaaattcgcg ttaaattttt gttaaatcag
     8521 ctcatttttt aaccaatagg ccgaaatcgg caaaatccct tataaatcaa aagaatagac
     8581 cgagataggg ttgagtgttg ttccagtttg gaacaagagt ccactattaa agaacgtgga
     8641 ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat ggcccactac gtgaaccatc
     8701 accctaatca agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg
     8761 gagcccccga tttagagctt gacggggaaa gccggcgaac gtggcgagaa aggaagggaa
     8821 gaaagcgaaa ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac
     8881 caccacaccc gccgcgctta atgcgccgct acagggcgcg tcaggtggca cttttcgggg
     8941 aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct
     9001 catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat
     9061 tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc
     9121 tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg
     9181 ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg
     9241 ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga
     9301 cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta
     9361 ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc
     9421 tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc
     9481 gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg
     9541 ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc
     9601 aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca
     9661 acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct
     9721 tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat
     9781 cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg
     9841 gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat
     9901 taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact
     9961 tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat
    10021 cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc
    10081 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct
    10141 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg
    10201 cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca
    10261 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc
    10321 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga
    10381 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac
    10441 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga
    10501 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
    10561 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
    10621 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
    10681 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc
    10741 tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc
    10801 tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc
    10861 aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag
    10921 gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt agctcactca
    10981 ttaggcaccc caggctttac actttatgct tccggctcgt atgttgtgtg gaattgtgag
    11041 cggataacaa tttcacacag gaaacagcta tgaccatgat tacgccaagc gcgcaattaa
    11101 ccctcactaa agggaacaaa agctgggtac cgggcccccc ctcgaattac cctgttatcc
    11161 ctatcgagca aaatcaatcc taatgtaaaa tagtatacgt tcatcattat aatgcaaaca
    11221 ctgtagaact gttgtgttgt gccttgcatt tcattctcag tttcttatcg tctgtctctt
    11281 tttacgacag agctagaaaa aactagcctt catttgccat cgccgtatgc aaatccactc
    11341 cagaactcaa caggtttgtt ctgcggcggg tgtaagtgtg aacaccggca tgtaagtgcg
    11401 cttgagcagg agaataaagc gctcagagac accggaggaa tcctttattt tgcgttgcgc
    11461 ctcaggggat tcgctgagcc ttctcttcat ggccctgctc ttcagacaca ggtgctccgc
    11521 tgtttgtgtt tttcactgtt cttcag
//
