LOCUS       sequence-44354-e         777 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses Mid2(SS/TM), containing only the signal sequence and 
            transmembrane helix, fused to GFP and LOVpep(T406A, T407A, I532A 
            mutations)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 777)
  AUTHORS   Strickland D, Lin Y, Wagner E, Hope CM, Zayner J, Antoniou C, 
            Sosnick TR, Weiss EL, Glotzer M
  TITLE     TULIPs: tunable, light-controlled interacting protein tags for cell 
            biology.
  JOURNAL   Nat Methods. 2012 Mar 4. doi: 10.1038/nmeth.1904.
  PUBMED    22388287
REFERENCE   2  (bases 1 to 777)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2012 Mar 4. doi: 10.1038/nmeth.1904."
COMMENT     Sequence Label: pDS275
FEATURES             Location/Qualifiers
     source          1..777
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(338..366)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
ORIGIN
        1 ggccgcacta gtggatcccc cgggctgcag gaattcgata tcaagcttat cgataccgtc
       61 gacaatgttg tctttcacaa ccaagaatag tttccgctta ttacttttaa tactgtcatg
      121 catatcgacg atacgcgcac aatttttcgt gcaatcatca tcttcgaatt cgggtctttc
      181 taaaaaaaat agaaacatcg tcattggttg tgtggttggt attggtgtac cactgattct
      241 ggtgatactg gctttaattt acatgttttg tatccaatca tcaaggacgg acgcgtcgac
      301 ctcgacaatg agtaaaggag aagaactttt cactggagtt gtcccaattc ttgttgaatt
      361 agatggtgat gttaatgggc acaaattttc tgtcagtgga gagggtgaag gtgatgcaac
      421 atacggaaaa cttaccctta aatttatttg cactactgga aaactacctg ttccatggcc
      481 aacacttgtc actaccttca cttatggtgt tcaatgcttt tcaagatacc cagatcatat
      541 gaaacggcat gactttttca agagtgccat gcccgaaggt tatgtacagg aaagaactat
      601 atttttcaaa gatgacggga actacaagac acgtgctgaa gtcaagtttg aaggtgatac
      661 ccttgttaat agaatcgagt taaaaggtat tgattttaaa gaagatggaa acattcttgg
      721 acacaaattg gaatacaact ataactcaca caatgtatac atcatggcag acaaaca
//
