LOCUS       sequence-443544-        6825 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Donor plasmid of hAT-9_XT Transposon.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6825)
  AUTHORS   Zhang T, Tan S, Tang N, Li Y, Zhang C, Sun J, Guo Y, Gao H, Cai Y, 
            Sun W, Wang C, Fu L, Ma H, Wu Y, Hu X, Zhang X, Gee P, Yan W, Zhao 
            Y, Chen Q, Guo B, Wang H, Zhang YE
  TITLE     Heterologous survey of 130 DNA transposons in human cells highlights
            their functional divergence and expands the genome engineering 
            toolbox.
  JOURNAL   Cell. 2024 Jul 11;187(14):3741-3760.e30. doi: 
            10.1016/j.cell.2024.05.007. Epub 2024 Jun 5.
  PUBMED    38843831
REFERENCE   2  (bases 1 to 6825)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2024.05.007"; journalName: "Cell"; date: 
            "2024-07-11- 11"; volume: "187"; issue: "14"; pages: "3741-3760.e30"
COMMENT     Sequence Label: pMV-hAT-9_XT
FEATURES             Location/Qualifiers
     source          1..6825
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     94..112
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     121..138
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     122..138
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        1269..1768
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(1304..1327)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     1701..1720
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     regulatory      1782..1791
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1788..2387
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MGTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIE
                     RVTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRL
                     AAQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLET
                     SAPRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     2287..2307
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     CDS             2394..2450
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             join(2451..2453,2454..2456,2457..3170)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(2451..2453,2454..2456,2457..3167)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2496..2517)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2757..2776)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3104..3125
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(3192..3209)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    3198..3422
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(4928..4944)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4928..4944)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4956..4973)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5127..5715)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5207..5226)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5886..6677,6678..6746))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLRNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERKRQIAEIGAS
                     LIKHW"
     primer_bind     6509..6528
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(join(6747..6825,1..26))
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa
       61 gaaaccatta ttatcatgac attaacctat aaaaataggc gtatcacgag gccctttcgt
      121 tgtaaaacga cggccagtcg aaccacgcaa tgcgtctcga tccgcagtgt cttgcgtctc
      181 taagcttatc atcattaggg atgtagcgaa ctgttcgccg gcgaactaat tcgcgcgaac
      241 atcgggtgtt cgcgaacccg caagttcgcg aacttttggc gtatgttcgc catttgggtt
      301 cgccttagct ggcgccaaat tttgacctct caccccagac cagcagatac atggcagcca
      361 atcaggcagc tctccctcct gggccacccc cccccccttg gaccactccc ttccatatat
      421 aaaccgaagc ccagcagcca ttttacattc tgcctgtgtg tgcttgaaga gatagtgtag
      481 ggagagagct gtgcattgat ttgagggaga gtttaagtag gttttctggc tagtaatcta
      541 ctttctactg ctctgtattt gtgtggggag aggaagctgt gcattgattt gagggagagt
      601 ttaagtaggt tttctggcta gtaatctact ttctactgct ctgtatttgt gtggggagag
      661 gaagctgtgc attgatttga gggagagttt aagtaggttt tctggctagt aatctacttc
      721 tactgctctg tatttgtgtg gggagaggag ctgtgtattg atttgaggca gagtttaagt
      781 aggttttctg gctagtaatc tactttctac tgctctgtat attttgtcta aataacaatt
      841 tgtctaaata acaataataa ttccgtgtcc agaaacatca cctgagtgac ggttttccac
      901 cagcaataat atattccgta tccactactg tatacgttgc ccttgcaggc cttgttgccc
      961 ggtgtctgca accaagtgcc acctagctgt gtgagctttt tcacaatctg tctaaataat
     1021 aataattccg tgtccagaaa catcacctga gtgacggttt tccaccagca ataatatatt
     1081 ccgtatccac tactgtatac gttgcccttg caggccttgt tgcccggtgt ctgcaaccaa
     1141 gtgccaccta gctgtgtgag ctttttcaca atctgtctaa ataacaataa taattccgtg
     1201 tccagaaaca tcacccaagt tgttgttgtt ttgtaaaaat aaaaaaaacc ggtgctagca
     1261 attctaccgg gtaggggagg cgcttttccc aaggcagtct ggagcatgcg ctttagcagc
     1321 cccgctgggc acttggcgct acacaagtgg cctctggcct cgcacacatt ccacatccac
     1381 cggtaggcgc caaccggctc cgttctttgg tggccccttc gcgccacctt ctactcctcc
     1441 cctagtcagg aagttccccc ccgccccgca gctcgcgtcg tgcaggacgt gacaaatgga
     1501 agtagcacgt ctcactagtc tcgtgcagat ggacagcacc gctgagcaat ggaagcgggt
     1561 aggcctttgg ggcagcggcc aatagcagct ttgctccttc gctttctggg ctcagaggct
     1621 gggaaggggt gggtccgggg gcgggctcag gggcgggctc aggggcgggg cgggcgcccg
     1681 aaggtcctcc ggaggcccgg cattctgcac gcttcaaaag cgcacgtctg ccgcgctgtt
     1741 ctcctcttcc tcatctccgg gcctttcgac ctgcagccaa cgccaccatg gggaccgagt
     1801 acaagcccac ggtgcgcctc gccacccgcg acgacgtccc ccgggccgta cgcaccctcg
     1861 ccgccgcgtt cgccgactac cccgccacgc gccacaccgt cgacccggac cgccacatcg
     1921 agcgggtcac cgagctgcaa gaactcttcc tcacgcgcgt cgggctcgac atcggcaagg
     1981 tgtgggtcgc ggacgacggc gccgcggtgg cggtctggac cacgccggag agcgtcgaag
     2041 cgggggcggt gttcgccgag atcggcccgc gcatggccga gttgagcggt tcccggctgg
     2101 ccgcgcagca acagatggaa ggcctcctgg cgccgcaccg gcccaaggag cccgcgtggt
     2161 tcctggccac cgtcggcgtc tcgcccgacc accagggcaa gggtctgggc agcgccgtcg
     2221 tgctccccgg agtggaggcg gccgagcgcg ccggggtgcc cgccttcctg gagacctccg
     2281 cgccccgcaa cctccccttc tacgagcggc tcggcttcac cgtcaccgcc gacgtcgagg
     2341 tgcccgaagg accgcgcacc tggtgcatga cccgcaagcc cggtgccatc gatgccacta
     2401 acttctccct gttgaaacaa gcaggggatg tcgaagagaa tcccgggcca atggtgagca
     2461 agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa
     2521 acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac ggcaagctga
     2581 ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca
     2641 ccctgaccta cggcgtgcag tgcttcagcc gctaccccga ccacatgaag cagcacgact
     2701 tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc ttcaaggacg
     2761 acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg gtgaaccgca
     2821 tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac aagctggagt
     2881 acaactacaa cagccacaac gtctatatca tggccgacaa gcagaagaac ggcatcaagg
     2941 tgaacttcaa gatccgccac aacatcgagg acggcagcgt gcagctcgcc gaccactacc
     3001 agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac tacctgagca
     3061 cccagtccgc cctgagcaaa gaccccaacg agaagcgcga tcacatggtc ctgctggagt
     3121 tcgtgaccgc cgccgggatc actctcggca tggacgagct gtacaagtaa ttaattaagc
     3181 tcgctgatca gcctcgactg tgccttctag ttgccagcca tctgttgttt gcccctcccc
     3241 cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc ctttcctaat aaaatgagga
     3301 aattgcatcg cattgtctga gtaggtgtca ttctattctg gggggtgggg tggggcagga
     3361 cagcaagggg gaggattggg aagacaatag caggcatgct ggggatgcgg tgggctctat
     3421 ggcttctgag gcgcgccctc gagctgccca tgcagctgtc cttctctgca cgccgcttga
     3481 caccacacac agctgtcctt tagcgtcctc ctccaccacc gtacaaacta gggtgcaaat
     3541 cctactggct taattttaag ccaaactttt tggacaggta aatcctgaat ttttctgtct
     3601 tctgtgcttg gcacgctatc ttagtttttt taaagggttt gcctggggca tggttatgat
     3661 accatctatc taagatgttt tttctgtctt ctgtgcttgg caccctatct tagttctttg
     3721 aaagggtttg cctggggcat ggttatgata ccatctagct ttgatgtttt ttctgtcttc
     3781 tgtgcttgtc accctatctt agttctttga aagggtttgc ctggggcatg gttatgatac
     3841 catctagctt tgatgttttt tctgtcttct gtgcttggca ccctatctta gttctttgaa
     3901 agggtttgcc tggggcatgg ttatgatacc atctatcttt gatgtttttt ctgtcttctg
     3961 tgcttggcac gctatcttag ttttttgaaa gggttctgcc tggggaatgg ttatgatacc
     4021 atctatctaa catatttttt ctgtcctctg tgcttcagtg gctgcgacaa caaaaataca
     4081 aactttttca acatttatct aacatatttt ttctgtcctc tgtgcttcag tggctgcaac
     4141 aaaaaaaaca taatttttca ggaatgtaca cattcctgat ttttcagggt tctgcaacag
     4201 cggcaaaatc gtatctttta tggtcaccac aggtgatcaa aaaggtagga caaaactggg
     4261 cccacactgc agaatcagtg ttttttggtt cacgtcactg tacattgaat tacctctgcc
     4321 tgaccgtgca cgtgcgcaca agcacggtga ctgctaaaca caccactaca gaaatattcc
     4381 caccgacagg acgaacgtcc tggaggtgac aagcaactag taaaaactat tattcgctca
     4441 cttgacagta tcattaaagc tttttgcgtt ttttttcgtt gcagtaaacg cggcgttttg
     4501 tctttgcgtg tgaaccggcc gtaaccttta cacgacttga ttggcatgta gacgccggac
     4561 tttttaaagc agtttattac ataagtttag gaatgtagtg tgatttctgc cctttacagc
     4621 acaaaacgca acgctgtgtc aacaacgtat ttttcagaga aatttttgcc cttgatcccc
     4681 ctcctgcatg ccactgtcca ggtcgtggca ccctttaaac aactttaaaa tcagttttct
     4741 ggccagaaat ggcttttcta ggttttaaag ttcgccttcc cattgaagtc tatggggttc
     4801 gcaaagttcg caaaagttcg cactttttgg cggaagttcg cgaacgggtt cgcgaacttt
     4861 ttttgtgagg ttcgctacat ctctaatcat catgggccca gagacggagt cactgccaac
     4921 cgagacggtc atagctgttt cctgtgtgcc gcttcctcgc tcactgactc gctgcgctcg
     4981 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttacccaca
     5041 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
     5101 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
     5161 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
     5221 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
     5281 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
     5341 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
     5401 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
     5461 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
     5521 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt
     5581 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
     5641 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
     5701 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
     5761 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
     5821 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct
     5881 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct ctttcgttca
     5941 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct
     6001 ggccccagtg ctgcaataat accgcgggac ccacgctcac cggctccaga tttatcagca
     6061 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc
     6121 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg
     6181 cgcaacgttg ttgccatcgc tacaggcatc gtggtatcac gctcgtcgtt tggtatggct
     6241 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgcgcaaa
     6301 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgccgtgtta
     6361 tcactcatgg ttatggcagc actacataat tctcttactg tcatgccatc cgtaagatgc
     6421 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg
     6481 agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa
     6541 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg
     6601 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc
     6661 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg
     6721 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat
     6781 cagggttatt gtctcatgag cggatacata tttgaatgta tttag
//
