LOCUS       sequence-44355-e         777 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses Mid2(SS/TM), containing only the signal sequence and 
            transmembrane helix, fused to GFP and LOVpep(V529N mutation)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 777)
  AUTHORS   Strickland D, Lin Y, Wagner E, Hope CM, Zayner J, Antoniou C, 
            Sosnick TR, Weiss EL, Glotzer M
  TITLE     TULIPs: tunable, light-controlled interacting protein tags for cell 
            biology.
  JOURNAL   Nat Methods. 2012 Mar 4. doi: 10.1038/nmeth.1904.
  PUBMED    22388287
REFERENCE   2  (bases 1 to 777)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2012 Mar 4. doi: 10.1038/nmeth.1904."
COMMENT     Sequence Label: pDS277
FEATURES             Location/Qualifiers
     source          1..777
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(339..367)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
ORIGIN
        1 cggccgcact agtggatccc ccgggctgca ggaattcgat atcaagctta tcgataccgt
       61 cgacaatgtt gtctttcaca accaagaata gtttccgctt attactttta atactgtcat
      121 gcatatcgac gatacgcgca caatttttcg tgcaatcatc atcttcgaat tcgggtcttt
      181 ctaaaaaaaa tagaaacatc gtcattggtt gtgtggttgg tattggtgta ccactgattc
      241 tggtgatact ggctttaatt tacatgtttt gtatccaatc atcaaggacg gacgcgtcga
      301 cctcgacaat gagtaaagga gaagaacttt tcactggagt tgtcccaatt cttgttgaat
      361 tagatggtga tgttaatggg cacaaatttt ctgtcagtgg agagggtgaa ggtgatgcaa
      421 catacggaaa acttaccctt aaatttattt gcactactgg aaaactacct gttccatggc
      481 caacacttgt cactaccttc acttatggtg ttcaatgctt ttcaagatac ccagatcata
      541 tgaaacggca tgactttttc aagagtgcca tgcccgaagg ttatgtacag gaaagaacta
      601 tatttttcaa agatgacggg aactacaaga cacgtgctga agtcaagttt gaaggtgata
      661 cccttgttaa tagaatcgag ttaaaaggta ttgattttaa agaagatgga aacattcttg
      721 gacacaaatt ggaatacaac tataactcac acaatgtata catcatggca gacaaac
//
