LOCUS       sequence-443560-        5525 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Donor plasmid of hAT-2_Gav Transposon.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5525)
  AUTHORS   Zhang T, Tan S, Tang N, Li Y, Zhang C, Sun J, Guo Y, Gao H, Cai Y, 
            Sun W, Wang C, Fu L, Ma H, Wu Y, Hu X, Zhang X, Gee P, Yan W, Zhao 
            Y, Chen Q, Guo B, Wang H, Zhang YE
  TITLE     Heterologous survey of 130 DNA transposons in human cells highlights
            their functional divergence and expands the genome engineering 
            toolbox.
  JOURNAL   Cell. 2024 Jul 11;187(14):3741-3760.e30. doi: 
            10.1016/j.cell.2024.05.007. Epub 2024 Jun 5.
  PUBMED    38843831
REFERENCE   2  (bases 1 to 5525)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2024.05.007"; journalName: "Cell"; date: 
            "2024-07-11- 11"; volume: "187"; issue: "14"; pages: "3741-3760.e30"
COMMENT     Sequence Label: pMV-hAT-2_Gav
FEATURES             Location/Qualifiers
     source          1..5525
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     94..112
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     121..138
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     122..138
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        1260..1759
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(1295..1318)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     1692..1711
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     regulatory      1773..1782
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1779..2378
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MGTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIE
                     RVTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRL
                     AAQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLET
                     SAPRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     2278..2298
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     CDS             2385..2441
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             join(2442..2444,2445..2447,2448..3161)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(2442..2444,2445..2447,2448..3158)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2487..2508)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2748..2767)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3095..3116
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(3183..3200)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    3189..3413
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(3628..3644)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3628..3644)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3656..3673)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3827..4415)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3907..3926)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4586..5377,5378..5446))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLRNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERKRQIAEIGAS
                     LIKHW"
     primer_bind     5209..5228
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(join(5447..5525,1..26))
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa
       61 gaaaccatta ttatcatgac attaacctat aaaaataggc gtatcacgag gccctttcgt
      121 tgtaaaacga cggccagtcg aaccacgcaa tgcgtctcga tccgcagtgt cttgcgtctc
      181 tcaattgcct tgaagcaagg ttccctctaa gctgcgcacg ctttaaaacc atgccgcgca
      241 cgaactcatt aatgccatgc acccacccgg tgaggagctg ggctgcggct tcgagaggca
      301 acaagctgag cacggcagag gcaccgaggc cggcatggga cacttaccag aggtaagctc
      361 aggcagcagc tcccgaggcc atgcgctgct ggaggcgggg cggggacagg ggctgggctg
      421 gggaactgct ccgcttccca gccccagtgc catgtgcctc ctcgctccca gaggcatggc
      481 gaggcgggga ctggggctgg cgtgggctct gccggcgcca gaaaactgct ccgctcccca
      541 gccccaatgc cacatgcctc ctcgctcctg gaggtgaggc aaggggcggg ggctgggaaa
      601 ctgctctgct cctgtggcga gcaggggccg gggctgggcc gacggctggg ccaggcctcg
      661 gggctggggc tgaggccggg aaactgcctg ctcccagcag tgaggcaggg gccaggacca
      721 ggaccgggaa actgctccac tcctgggaca atgcgggggc tggggctggg ccgggccaga
      781 ctcctgctcc agggcaggca aggcgggggc tggggcagga ccggaaactg ctccgctccc
      841 agaggcaatg cggggacagg ggctgaggtt cctggctttg aggggccttc gctattaagt
      901 gccattattt tgcatgtaaa gtaagtggac cagttaattt aatgaatttc caaactgcaa
      961 actaaataac aaagccatgt gcagactgta gagggaaaat taaatttatt ctgaagtgtg
     1021 ttgttatttt tcgatcatcc agtcatgtta taagacatga aaggattata attctagtaa
     1081 tggcaataca caactctact aatatgcatt cctatagaat tggggggagc gggtctgcgc
     1141 tcccaggagg ctagggcttg tgttagctgc tttttctatc attctaggaa ggccaatatc
     1201 ttccctgcag caaggatcat ctttatattc attttacaac cggtgctagc aattctaccg
     1261 ggtaggggag gcgcttttcc caaggcagtc tggagcatgc gctttagcag ccccgctggg
     1321 cacttggcgc tacacaagtg gcctctggcc tcgcacacat tccacatcca ccggtaggcg
     1381 ccaaccggct ccgttctttg gtggcccctt cgcgccacct tctactcctc ccctagtcag
     1441 gaagttcccc cccgccccgc agctcgcgtc gtgcaggacg tgacaaatgg aagtagcacg
     1501 tctcactagt ctcgtgcaga tggacagcac cgctgagcaa tggaagcggg taggcctttg
     1561 gggcagcggc caatagcagc tttgctcctt cgctttctgg gctcagaggc tgggaagggg
     1621 tgggtccggg ggcgggctca ggggcgggct caggggcggg gcgggcgccc gaaggtcctc
     1681 cggaggcccg gcattctgca cgcttcaaaa gcgcacgtct gccgcgctgt tctcctcttc
     1741 ctcatctccg ggcctttcga cctgcagcca acgccaccat ggggaccgag tacaagccca
     1801 cggtgcgcct cgccacccgc gacgacgtcc cccgggccgt acgcaccctc gccgccgcgt
     1861 tcgccgacta ccccgccacg cgccacaccg tcgacccgga ccgccacatc gagcgggtca
     1921 ccgagctgca agaactcttc ctcacgcgcg tcgggctcga catcggcaag gtgtgggtcg
     1981 cggacgacgg cgccgcggtg gcggtctgga ccacgccgga gagcgtcgaa gcgggggcgg
     2041 tgttcgccga gatcggcccg cgcatggccg agttgagcgg ttcccggctg gccgcgcagc
     2101 aacagatgga aggcctcctg gcgccgcacc ggcccaagga gcccgcgtgg ttcctggcca
     2161 ccgtcggcgt ctcgcccgac caccagggca agggtctggg cagcgccgtc gtgctccccg
     2221 gagtggaggc ggccgagcgc gccggggtgc ccgccttcct ggagacctcc gcgccccgca
     2281 acctcccctt ctacgagcgg ctcggcttca ccgtcaccgc cgacgtcgag gtgcccgaag
     2341 gaccgcgcac ctggtgcatg acccgcaagc ccggtgccat cgatgccact aacttctccc
     2401 tgttgaaaca agcaggggat gtcgaagaga atcccgggcc aatggtgagc aagggcgagg
     2461 agctgttcac cggggtggtg cccatcctgg tcgagctgga cggcgacgta aacggccaca
     2521 agttcagcgt gtccggcgag ggcgagggcg atgccaccta cggcaagctg accctgaagt
     2581 tcatctgcac caccggcaag ctgcccgtgc cctggcccac cctcgtgacc accctgacct
     2641 acggcgtgca gtgcttcagc cgctaccccg accacatgaa gcagcacgac ttcttcaagt
     2701 ccgccatgcc cgaaggctac gtccaggagc gcaccatctt cttcaaggac gacggcaact
     2761 acaagacccg cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc atcgagctga
     2821 agggcatcga cttcaaggag gacggcaaca tcctggggca caagctggag tacaactaca
     2881 acagccacaa cgtctatatc atggccgaca agcagaagaa cggcatcaag gtgaacttca
     2941 agatccgcca caacatcgag gacggcagcg tgcagctcgc cgaccactac cagcagaaca
     3001 cccccatcgg cgacggcccc gtgctgctgc ccgacaacca ctacctgagc acccagtccg
     3061 ccctgagcaa agaccccaac gagaagcgcg atcacatggt cctgctggag ttcgtgaccg
     3121 ccgccgggat cactctcggc atggacgagc tgtacaagta attaattaag ctcgctgatc
     3181 agcctcgact gtgccttcta gttgccagcc atctgttgtt tgcccctccc ccgtgccttc
     3241 cttgaccctg gaaggtgcca ctcccactgt cctttcctaa taaaatgagg aaattgcatc
     3301 gcattgtctg agtaggtgtc attctattct ggggggtggg gtggggcagg acagcaaggg
     3361 ggaggattgg gaagacaata gcaggcatgc tggggatgcg gtgggctcta tggcttctga
     3421 ggcgcgccct cgagataaag gttatagtat catcttttgt tgttatactg tgttatactg
     3481 tggggcagac caaagtggcg cgcgccgaaa gggggaggcg ggaccaattc gggaatggac
     3541 cgaagcggcg tgctcacgga ccgctacacc ttagagggaa cattgccttg aaggggccca
     3601 gagacggagt cactgccaac cgagacggtc atagctgttt cctgtgtgcc gcttcctcgc
     3661 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg
     3721 cggtaatacg gttacccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag
     3781 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc
     3841 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
     3901 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
     3961 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
     4021 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
     4081 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
     4141 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
     4201 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca
     4261 ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag
     4321 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca
     4381 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg
     4441 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa
     4501 aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta
     4561 tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag
     4621 cgatctgtct ctttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga
     4681 tacgggaggg cttaccatct ggccccagtg ctgcaataat accgcgggac ccacgctcac
     4741 cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc
     4801 ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta
     4861 gttcgccagt taatagtttg cgcaacgttg ttgccatcgc tacaggcatc gtggtatcac
     4921 gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat
     4981 gatcccccat gttgcgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa
     5041 gtaagttggc cgccgtgtta tcactcatgg ttatggcagc actacataat tctcttactg
     5101 tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag
     5161 aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc
     5221 cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct
     5281 caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat
     5341 cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg
     5401 ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc
     5461 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta
     5521 tttag
//
