LOCUS       sequence-443567-        7162 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Donor plasmid of Tigger1 Transposon.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7162)
  AUTHORS   Zhang T, Tan S, Tang N, Li Y, Zhang C, Sun J, Guo Y, Gao H, Cai Y, 
            Sun W, Wang C, Fu L, Ma H, Wu Y, Hu X, Zhang X, Gee P, Yan W, Zhao 
            Y, Chen Q, Guo B, Wang H, Zhang YE
  TITLE     Heterologous survey of 130 DNA transposons in human cells highlights
            their functional divergence and expands the genome engineering 
            toolbox.
  JOURNAL   Cell. 2024 Jul 11;187(14):3741-3760.e30. doi: 
            10.1016/j.cell.2024.05.007. Epub 2024 Jun 5.
  PUBMED    38843831
REFERENCE   2  (bases 1 to 7162)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2024.05.007"; journalName: "Cell"; date: 
            "2024-07-11- 11"; volume: "187"; issue: "14"; pages: "3741-3760.e30"
COMMENT     Sequence Label: pMV-Tigger1
FEATURES             Location/Qualifiers
     source          1..7162
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     94..112
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     121..138
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     122..138
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        2046..2545
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(2081..2104)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     2478..2497
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     regulatory      2559..2568
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2565..3164
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MGTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIE
                     RVTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRL
                     AAQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLET
                     SAPRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     3064..3084
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     CDS             3171..3227
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             join(3228..3230,3231..3233,3234..3947)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(3228..3230,3231..3233,3234..3944)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3273..3294)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3534..3553)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3881..3902
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(3969..3986)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    3975..4199
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(5265..5281)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5265..5281)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5293..5310)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5464..6052)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5544..5563)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6223..7014,7015..7083))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLRNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERKRQIAEIGAS
                     LIKHW"
     primer_bind     6846..6865
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(join(7084..7162,1..26))
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa
       61 gaaaccatta ttatcatgac attaacctat aaaaataggc gtatcacgag gccctttcgt
      121 tgtaaaacga cggccagtcg aaccacgcaa tgcgtctcga tccgcagtgt cttgcgtctc
      181 tcaattgtac aggcatacct cgttttattg cgcttcgctt tattgcgctt cgcagatatt
      241 gcgtttttta caaattgaag gtttgtggca accctgcgtc gagcaagtct atcggcgcca
      301 tttttccaac agcatgtgct cacttcgtgt ctctgtgtca cattttggta attctcgcaa
      361 tatttcaaac tttttcatta ttattatatc tgttatggtg atctgtgatc agtgatcttt
      421 gatgttacta ttgtaattgt tttggggcgc cacgaaccgc gcccatataa gacggcgaac
      481 ttaatcgata aatgttgtgt gtgttctgac tgctccaccg accggccgtt cccccatctc
      541 tctccctctc ctcgggcctc cctattccct gagacacaac aatattgaaa ttaggccaat
      601 taataaccct acaaccggtc aattgtttcc ttgcagggct gcccagaggg gggggggggc
      661 aactggggca atttgccccg ggcccctgca aagcaagggc cccctgcctc agctcctgtg
      721 tgtaccgttg gcccctgccc tgcgacatcg ctcaccagaa ttcttggtat tttgttttca
      781 tgaaaaagag gaaattggtg tattagagtg taagatgaca ctgaatcatt ctcaacatgt
      841 aattgtgaca ttatgggacc cgtccccgac catgaagtaa atattataca tagttgtgtg
      901 tgaaacagat gctaatttga ggcttaagtg attgttgcat tgatgtccaa gggggagtct
      961 aaaccaatca cggtaaggta acatctttct ttatctctgg gcagcaatag agacaagatt
     1021 gatcaggcag gcttatgatt ggcctagcct agggcctagg ccaagtagcc ctggttacag
     1081 agtctgttgt gaggagagaa ggaacaggga aggctttaag gagggatctg tggcagagcc
     1141 ggcctgttgt cacagaaaga cctccagggg aaagagttca agtcgtgagt gaatgtcagc
     1201 tctgtgggtt cttaagggcc aaagtcagaa atgaatttgc aagaatataa taattaaaat
     1261 gatatatatt gttttcttta tatttgccaa ggaaaataac aacattagac ataacacata
     1321 ttcaggttca catatttccc cacttcaaaa agtacttaaa tgcaaagtgt ctatataact
     1381 cttggggaaa aatattaaag tccttcttga ttgaagaagc ctgtgctcca aactgcaaat
     1441 cttgctttca gtattgcaga gatgacttgc tctcttctgg tccttcaaac agcagcagtt
     1501 gcttactaag gcccataaga atatgaacag tctcacaatg cccttaatca aggggttaaa
     1561 ggctaaatat acaagtttat tctccagcaa cagtacaaag acaatcctcc ttcctctgtc
     1621 tactctctct gcagttactt tctttttcct taaacagggg cagggcctgc ttatcctctg
     1681 ccacacccac aagcctgaac caagtacaac ccagcttatg ggggttacat gagcaccagc
     1741 taagatttcc aggcaaggtc ccactggaga gtaattttct gtggcagtta ccacagggaa
     1801 aaggcctagg agtggtgtga ggccaggcct taagggagga atccttcctt cctgagtagt
     1861 ggaggaggcc agccagcagg ccacaaccag aagacctgtg agactgagcc gggccccagg
     1921 aaactggtag gagagagaag ctctgccaga aggggtttgg aatttggagg tgctcaggag
     1981 aagtacaacc ctattcctat ccccttatcc ccgtgtaaga aaaaaccggt gctagcaatt
     2041 ctaccgggta ggggaggcgc ttttcccaag gcagtctgga gcatgcgctt tagcagcccc
     2101 gctgggcact tggcgctaca caagtggcct ctggcctcgc acacattcca catccaccgg
     2161 taggcgccaa ccggctccgt tctttggtgg ccccttcgcg ccaccttcta ctcctcccct
     2221 agtcaggaag ttcccccccg ccccgcagct cgcgtcgtgc aggacgtgac aaatggaagt
     2281 agcacgtctc actagtctcg tgcagatgga cagcaccgct gagcaatgga agcgggtagg
     2341 cctttggggc agcggccaat agcagctttg ctccttcgct ttctgggctc agaggctggg
     2401 aaggggtggg tccgggggcg ggctcagggg cgggctcagg ggcggggcgg gcgcccgaag
     2461 gtcctccgga ggcccggcat tctgcacgct tcaaaagcgc acgtctgccg cgctgttctc
     2521 ctcttcctca tctccgggcc tttcgacctg cagccaacgc caccatgggg accgagtaca
     2581 agcccacggt gcgcctcgcc acccgcgacg acgtcccccg ggccgtacgc accctcgccg
     2641 ccgcgttcgc cgactacccc gccacgcgcc acaccgtcga cccggaccgc cacatcgagc
     2701 gggtcaccga gctgcaagaa ctcttcctca cgcgcgtcgg gctcgacatc ggcaaggtgt
     2761 gggtcgcgga cgacggcgcc gcggtggcgg tctggaccac gccggagagc gtcgaagcgg
     2821 gggcggtgtt cgccgagatc ggcccgcgca tggccgagtt gagcggttcc cggctggccg
     2881 cgcagcaaca gatggaaggc ctcctggcgc cgcaccggcc caaggagccc gcgtggttcc
     2941 tggccaccgt cggcgtctcg cccgaccacc agggcaaggg tctgggcagc gccgtcgtgc
     3001 tccccggagt ggaggcggcc gagcgcgccg gggtgcccgc cttcctggag acctccgcgc
     3061 cccgcaacct ccccttctac gagcggctcg gcttcaccgt caccgccgac gtcgaggtgc
     3121 ccgaaggacc gcgcacctgg tgcatgaccc gcaagcccgg tgccatcgat gccactaact
     3181 tctccctgtt gaaacaagca ggggatgtcg aagagaatcc cgggccaatg gtgagcaagg
     3241 gcgaggagct gttcaccggg gtggtgccca tcctggtcga gctggacggc gacgtaaacg
     3301 gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc aagctgaccc
     3361 tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc
     3421 tgacctacgg cgtgcagtgc ttcagccgct accccgacca catgaagcag cacgacttct
     3481 tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac catcttcttc aaggacgacg
     3541 gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga caccctggtg aaccgcatcg
     3601 agctgaaggg catcgacttc aaggaggacg gcaacatcct ggggcacaag ctggagtaca
     3661 actacaacag ccacaacgtc tatatcatgg ccgacaagca gaagaacggc atcaaggtga
     3721 acttcaagat ccgccacaac atcgaggacg gcagcgtgca gctcgccgac cactaccagc
     3781 agaacacccc catcggcgac ggccccgtgc tgctgcccga caaccactac ctgagcaccc
     3841 agtccgccct gagcaaagac cccaacgaga agcgcgatca catggtcctg ctggagttcg
     3901 tgaccgccgc cgggatcact ctcggcatgg acgagctgta caagtaatta attaagctcg
     3961 ctgatcagcc tcgactgtgc cttctagttg ccagccatct gttgtttgcc cctcccccgt
     4021 gccttccttg accctggaag gtgccactcc cactgtcctt tcctaataaa atgaggaaat
     4081 tgcatcgcat tgtctgagta ggtgtcattc tattctgggg ggtggggtgg ggcaggacag
     4141 caagggggag gattgggaag acaatagcag gcatgctggg gatgcggtgg gctctatggc
     4201 ttctgaggcg cgccctcgag actttcctct tctcattttg ttcttaagtt cctaactttg
     4261 tagcataagt ttaataaatg tttcacttgc aaactgttaa tgtcattttg ctttagtaac
     4321 ctgtctccaa ggtaaattct ctctgcctag ccctccttgc cacgggaaca agcggggaaa
     4381 gcccacttgt tcccacttcc cagggcaagg gaaggaggag ccgcatgtga gaattctcac
     4441 atgcgatgcg tgcgtgcaag aattctcgca cgtggtcccc cagctttgta cacacacaca
     4501 cgggtccttg tagggggcag tggtggggcc cccaacatca cacctgcccc aggccccata
     4561 aaccctctgg gcagccctgt ttccttgggg cccctcgaga cggatgagga gttgcttctt
     4621 atggatgagc aaagaaagtg gtttcttgag atggaatcta ctcctggtga agatgctgtg
     4681 aacattgttg aaatgacaac aaaggattta gaatattaca taaacttagt tgataaagca
     4741 gcggcagggt ttgagaggat tgactccaat tttgaaagaa gttctactgt gggtaaaatg
     4801 ctatcaaaca gcatcgcatg ctacagagaa atctttcgtg aaaggaagag tcaatcgatg
     4861 cggcaaactt cattgttgtc ttattttaag aaattgccac agccacccca accttcagca
     4921 accaccaccc tgatcagtca gcagccatca acatcgaggc aagaccctcc accagcaaaa
     4981 agattacgac tcgctgaagg ctcagatgat cgttagcatt ttttagcaat aaagtatttt
     5041 taaattaagg tatgtacatt gttttttaga cataatgcta ttgcacactt aatagactac
     5101 agtatagtgt aaacataact tttatatgca ctgggaaacc aaaaaattcg tgtgactcgc
     5161 tttattgcga tattcgcttt attgcggtgg tctggaaccg aacccgcaat atctccgagg
     5221 tatgcctgta gggcccagag acggagtcac tgccaaccga gacggtcata gctgtttcct
     5281 gtgtgccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt
     5341 atcagctcac tcaaaggcgg taatacggtt acccacagaa tcaggggata acgcaggaaa
     5401 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
     5461 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
     5521 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
     5581 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
     5641 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg
     5701 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
     5761 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
     5821 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
     5881 gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt
     5941 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
     6001 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
     6061 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt
     6121 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt
     6181 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag
     6241 tgaggcacct atctcagcga tctgtctctt tcgttcatcc atagttgcct gactccccgt
     6301 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caataatacc
     6361 gcgggaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc
     6421 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg
     6481 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccatcgctac
     6541 aggcatcgtg gtatcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg
     6601 atcaaggcga gttacatgat cccccatgtt gcgcaaaaaa gcggttagct ccttcggtcc
     6661 tccgatcgtt gtcagaagta agttggccgc cgtgttatca ctcatggtta tggcagcact
     6721 acataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc
     6781 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat
     6841 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc
     6901 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac
     6961 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa
     7021 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact
     7081 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg
     7141 atacatattt gaatgtattt ag
//
