LOCUS       sequence-443572-        5385 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Donor plasmid of Tigger4 Transposon.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5385)
  AUTHORS   Zhang T, Tan S, Tang N, Li Y, Zhang C, Sun J, Guo Y, Gao H, Cai Y, 
            Sun W, Wang C, Fu L, Ma H, Wu Y, Hu X, Zhang X, Gee P, Yan W, Zhao 
            Y, Chen Q, Guo B, Wang H, Zhang YE
  TITLE     Heterologous survey of 130 DNA transposons in human cells highlights
            their functional divergence and expands the genome engineering 
            toolbox.
  JOURNAL   Cell. 2024 Jul 11;187(14):3741-3760.e30. doi: 
            10.1016/j.cell.2024.05.007. Epub 2024 Jun 5.
  PUBMED    38843831
REFERENCE   2  (bases 1 to 5385)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2024.05.007"; journalName: "Cell"; date: 
            "2024-07-11- 11"; volume: "187"; issue: "14"; pages: "3741-3760.e30"
COMMENT     Sequence Label: pMV-Tigger4
FEATURES             Location/Qualifiers
     source          1..5385
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     94..112
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     121..138
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     122..138
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        619..1118
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(654..677)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     1051..1070
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     regulatory      1132..1141
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1138..1737
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MGTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIE
                     RVTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRL
                     AAQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLET
                     SAPRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     1637..1657
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     CDS             1744..1800
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             join(1801..1803,1804..1806,1807..2520)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(1801..1803,1804..1806,1807..2517)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1846..1867)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2107..2126)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2454..2475
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(2542..2559)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    2548..2772
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(3488..3504)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3488..3504)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3516..3533)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3687..4275)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3767..3786)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4446..5237,5238..5306))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLRNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERKRQIAEIGAS
                     LIKHW"
     primer_bind     5069..5088
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(join(5307..5385,1..26))
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa
       61 gaaaccatta ttatcatgac attaacctat aaaaataggc gtatcacgag gccctttcgt
      121 tgtaaaacga cggccagtcg aaccacgcaa tgcgtctcga tccgcagtgt cttgcgtctc
      181 tcaattgtac aggttgagca tccctaatcc gaaaatccga aatccgaaat gctccaaaat
      241 ccgaaacttt ttgagcgccg acatgacgcc acaagtggaa aattccacac ctgacctcat
      301 gtgacgggtc gcagtcaaaa cgcaggcgca caacacacag tttattcagc gtccccaagg
      361 gaaaaaagac cctcccagcc cccttcagct gcgatatatc ttttccgcgc acgcccagat
      421 tcccccacgc aagcacgccc acaaagggta ataaaatggc acgtgtgcag gccggacgcg
      481 ccaacggcag gttccccacg atgccccaca tggggccaag acctacgtgc attactcact
      541 gtgttttttt tgttttttgc ttattctctg ctctgtggtg taaagatatt gttgaaaacc
      601 ggtgctagca attctaccgg gtaggggagg cgcttttccc aaggcagtct ggagcatgcg
      661 ctttagcagc cccgctgggc acttggcgct acacaagtgg cctctggcct cgcacacatt
      721 ccacatccac cggtaggcgc caaccggctc cgttctttgg tggccccttc gcgccacctt
      781 ctactcctcc cctagtcagg aagttccccc ccgccccgca gctcgcgtcg tgcaggacgt
      841 gacaaatgga agtagcacgt ctcactagtc tcgtgcagat ggacagcacc gctgagcaat
      901 ggaagcgggt aggcctttgg ggcagcggcc aatagcagct ttgctccttc gctttctggg
      961 ctcagaggct gggaaggggt gggtccgggg gcgggctcag gggcgggctc aggggcgggg
     1021 cgggcgcccg aaggtcctcc ggaggcccgg cattctgcac gcttcaaaag cgcacgtctg
     1081 ccgcgctgtt ctcctcttcc tcatctccgg gcctttcgac ctgcagccaa cgccaccatg
     1141 gggaccgagt acaagcccac ggtgcgcctc gccacccgcg acgacgtccc ccgggccgta
     1201 cgcaccctcg ccgccgcgtt cgccgactac cccgccacgc gccacaccgt cgacccggac
     1261 cgccacatcg agcgggtcac cgagctgcaa gaactcttcc tcacgcgcgt cgggctcgac
     1321 atcggcaagg tgtgggtcgc ggacgacggc gccgcggtgg cggtctggac cacgccggag
     1381 agcgtcgaag cgggggcggt gttcgccgag atcggcccgc gcatggccga gttgagcggt
     1441 tcccggctgg ccgcgcagca acagatggaa ggcctcctgg cgccgcaccg gcccaaggag
     1501 cccgcgtggt tcctggccac cgtcggcgtc tcgcccgacc accagggcaa gggtctgggc
     1561 agcgccgtcg tgctccccgg agtggaggcg gccgagcgcg ccggggtgcc cgccttcctg
     1621 gagacctccg cgccccgcaa cctccccttc tacgagcggc tcggcttcac cgtcaccgcc
     1681 gacgtcgagg tgcccgaagg accgcgcacc tggtgcatga cccgcaagcc cggtgccatc
     1741 gatgccacta acttctccct gttgaaacaa gcaggggatg tcgaagagaa tcccgggcca
     1801 atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac
     1861 ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac
     1921 ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc
     1981 ctcgtgacca ccctgaccta cggcgtgcag tgcttcagcc gctaccccga ccacatgaag
     2041 cagcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc
     2101 ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg
     2161 gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac
     2221 aagctggagt acaactacaa cagccacaac gtctatatca tggccgacaa gcagaagaac
     2281 ggcatcaagg tgaacttcaa gatccgccac aacatcgagg acggcagcgt gcagctcgcc
     2341 gaccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac
     2401 tacctgagca cccagtccgc cctgagcaaa gaccccaacg agaagcgcga tcacatggtc
     2461 ctgctggagt tcgtgaccgc cgccgggatc actctcggca tggacgagct gtacaagtaa
     2521 ttaattaagc tcgctgatca gcctcgactg tgccttctag ttgccagcca tctgttgttt
     2581 gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc ctttcctaat
     2641 aaaatgagga aattgcatcg cattgtctga gtaggtgtca ttctattctg gggggtgggg
     2701 tggggcagga cagcaagggg gaggattggg aagacaatag caggcatgct ggggatgcgg
     2761 tgggctctat ggcttctgag gcgcgccctc gagaaagcca tccagcagaa tgcctcctca
     2821 tccctagagg acccacttcc tggtccctca actgcttctg atgtttcttc tcacctaaaa
     2881 aataaaatac agtgtacagt aaccttttaa tcaaaacaca gcatcgtagg tggagactga
     2941 aagcctgccg ttgtttgttg ttgctgttgt ttaacagctg atacaggtat tctggtgatg
     3001 ctactgtgct gcttagttac cctgaacaca ttattttttc actgtattaa tggtatgtca
     3061 tattttttac tgttaagtac ttatgtgtga ataagtgtaa gaaaatgatt gcttatcggt
     3121 agcatataaa ttcagagtca ggaatgatgg tgatgccaaa caaccacaga ttgtccacat
     3181 gggtggctga gatagtgaca cctttgcttt ctgatggttc aatgtacaca aactttgttt
     3241 catgcacaaa attatttaaa atattgtata aaattacctt caggctatgt gtataaggtg
     3301 tatatgaaac ataaatgaat ttcgtgttta gacttgggtc ccatccccaa gatatctcat
     3361 tatgtatatg caaatattcc aaaatccgaa aaaatccgaa atccgaaaca cttctggtcc
     3421 caagcatttc ggataaggga tactcaacct gtagggccca gagacggagt cactgccaac
     3481 cgagacggtc atagctgttt cctgtgtgcc gcttcctcgc tcactgactc gctgcgctcg
     3541 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttacccaca
     3601 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
     3661 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
     3721 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
     3781 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
     3841 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
     3901 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
     3961 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
     4021 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
     4081 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt
     4141 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
     4201 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
     4261 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
     4321 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
     4381 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct
     4441 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct ctttcgttca
     4501 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct
     4561 ggccccagtg ctgcaataat accgcgggac ccacgctcac cggctccaga tttatcagca
     4621 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc
     4681 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg
     4741 cgcaacgttg ttgccatcgc tacaggcatc gtggtatcac gctcgtcgtt tggtatggct
     4801 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgcgcaaa
     4861 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgccgtgtta
     4921 tcactcatgg ttatggcagc actacataat tctcttactg tcatgccatc cgtaagatgc
     4981 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg
     5041 agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa
     5101 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg
     5161 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc
     5221 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg
     5281 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat
     5341 cagggttatt gtctcatgag cggatacata tttgaatgta tttag
//
