LOCUS       sequence-443579-        6711 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Donor plasmid of hAT-17_Croc Transposon.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6711)
  AUTHORS   Zhang T, Tan S, Tang N, Li Y, Zhang C, Sun J, Guo Y, Gao H, Cai Y, 
            Sun W, Wang C, Fu L, Ma H, Wu Y, Hu X, Zhang X, Gee P, Yan W, Zhao 
            Y, Chen Q, Guo B, Wang H, Zhang YE
  TITLE     Heterologous survey of 130 DNA transposons in human cells highlights
            their functional divergence and expands the genome engineering 
            toolbox.
  JOURNAL   Cell. 2024 Jul 11;187(14):3741-3760.e30. doi: 
            10.1016/j.cell.2024.05.007. Epub 2024 Jun 5.
  PUBMED    38843831
REFERENCE   2  (bases 1 to 6711)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2024.05.007"; journalName: "Cell"; date: 
            "2024-07-11- 11"; volume: "187"; issue: "14"; pages: "3741-3760.e30"
COMMENT     Sequence Label: pMV-hAT-17_Croc
FEATURES             Location/Qualifiers
     source          1..6711
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(791..807)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(791..807)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(819..836)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(990..1578)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1070..1089)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1749..2540,2541..2609))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLRNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERKRQIAEIGAS
                     LIKHW"
     primer_bind     2372..2391
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2610..2714)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2782..2800
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     2809..2826
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2810..2826
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        4063..4562
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(4098..4121)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     4495..4514
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     regulatory      4576..4585
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4582..5181
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MGTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIE
                     RVTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRL
                     AAQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLET
                     SAPRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     5081..5101
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     CDS             5188..5244
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             join(5245..5247,5248..5250,5251..5964)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(5245..5247,5248..5250,5251..5961)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(5290..5311)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(5551..5570)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5898..5919
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(5986..6003)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5992..6216
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
ORIGIN
        1 ccttgctaag atatatttct ctaactattc agttggtcta ctaaaagata ccagattaga
       61 ttgacccaaa gaaccttgtc tgccagtgcc acgtccttag accaacaccc ctagcacctg
      121 tccccggtca ggcgacaggc gggcacatct aagccccagg gctggggctg gcagggagag
      181 ttctctggca gctgctggag aagaagctcc tgcaggaaag gaaagaaagg ctctgtgaca
      241 gtttggctgc ctgagatgct gctggtgcta ctgtgctgct tactatttta cctgttgtta
      301 tttaaagtgg tggaccgggc tgaggctgta ggggaggagg agacctcatt ggggcacact
      361 accgtgtcgt gtagaggccc cagcgccagt gagggcgcgc cttaacacac acacaaagtc
      421 acacatgtca cgagttttgc ttgtcagcag cttgaggtgc agtttcccag aaacccctgc
      481 acctatctcc ttgaaacttg gcaggcttcg tggcctcagc aggggctacc atccctgctg
      541 ttttcatccg aatcaggcaa gaaatgacaa agttataggc agtttcgtga ttccccatta
      601 tagcctatgg gcgaaacgtc gaaacagcgt cgaaacagcg aaacagtttc gacgaaacga
      661 aacggaacga aactcgaaac gaaacactgt cccgtcgaaa cggcgaaacg gaagtcgaaa
      721 cgaaacggtg ctgtttcgca cagccctact gtataggggc ccagagacgg agtcactgcc
      781 aaccgagacg gtcatagctg tttcctgtgt gccgcttcct cgctcactga ctcgctgcgc
      841 tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttaccc
      901 acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg
      961 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat
     1021 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag
     1081 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga
     1141 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg
     1201 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt
     1261 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac
     1321 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc
     1381 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt
     1441 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc
     1501 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc
     1561 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg
     1621 aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag
     1681 atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg
     1741 tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg tctctttcgt
     1801 tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca
     1861 tctggcccca gtgctgcaat aataccgcgg gacccacgct caccggctcc agatttatca
     1921 gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc
     1981 tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt
     2041 ttgcgcaacg ttgttgccat cgctacaggc atcgtggtat cacgctcgtc gtttggtatg
     2101 gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgcgc
     2161 aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgccgtg
     2221 ttatcactca tggttatggc agcactacat aattctctta ctgtcatgcc atccgtaaga
     2281 tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga
     2341 ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta
     2401 aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg
     2461 ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact
     2521 ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata
     2581 agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt
     2641 tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa
     2701 ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtctaaga aaccattatt
     2761 atcatgacat taacctataa aaataggcgt atcacgaggc cctttcgttg taaaacgacg
     2821 gccagtcgaa ccacgcaatg cgtctcgatc cgcagtgtct tgcgtctctc aattgctgta
     2881 tagtagggct gtgcgaaatt tcgccggctg tttcgtttcg acgctgtttc gactcgtttc
     2941 gagctcgaaa cagcgaaatc gaaacgaaac gaagggcttc gaaacagcct cgaaacgaaa
     3001 cgagggcagt cgaaacgttt cgaaagtttc gaaacgtttc gagtttcgaa gctgaagctg
     3061 ggcttgcttg cagggaaaca gaaagtaagg agagggaggg ggaagagggg ggaaggactg
     3121 ctctgatatt gactgtgtag ctggccagtg actgtgatcc ttccctgagg tcccttccaa
     3181 tcccagtgct ctgggggagg gatggaaaaa cagcataaaa agcagcattg tttgaactga
     3241 gctgctttct gccttggggt cagttactga gctgtgtctt ccagcagctc agcagcgtca
     3301 gacaacaaag gctaccattc atttcctact tgctagccta gcaagtggga ttcatcaata
     3361 ccttttgctt tctttctata cccttttatt ttattattat attcattcat tattcctttc
     3421 aatttattcc tcccccaaaa tcctcttttt gtttttgtta gtctatttcg attcttttcc
     3481 cccagaaaag aaatctgtgc tttctctcac agtgtgcatt ccattcactg tgcagggaag
     3541 cacagcataa ttatattgca tattataata tagaatttat acaggaacac atacatacat
     3601 ttatatattt tattatttat tacataaata catacataca tacatacata cattacataa
     3661 atacacatac atatattatc tacatatatt acataaatca gattacatta cagaagaaca
     3721 cacagatttt ccagcacacc aaggcaaaga caagacacta tgaaacacac catgaagcgc
     3781 gctggaaagg cagctggcaa gccttcaggg aggggcggaa gagggggtag agggagagct
     3841 gtaagttccc cccccccccc aaactgagac gcagcctctt tcctacaaca agcaggcatg
     3901 aggcttccac tagtacaggc agcaaggaga ggggagcagt gccagtgcca ctgcctgtgc
     3961 ctgagtctgc atcccctcca agagcaccag ccataacccc cccccaccac caccacgaca
     4021 acaagcagca ccagcaccag caccggtgct agcaattcta ccgggtaggg gaggcgcttt
     4081 tcccaaggca gtctggagca tgcgctttag cagccccgct gggcacttgg cgctacacaa
     4141 gtggcctctg gcctcgcaca cattccacat ccaccggtag gcgccaaccg gctccgttct
     4201 ttggtggccc cttcgcgcca ccttctactc ctcccctagt caggaagttc ccccccgccc
     4261 cgcagctcgc gtcgtgcagg acgtgacaaa tggaagtagc acgtctcact agtctcgtgc
     4321 agatggacag caccgctgag caatggaagc gggtaggcct ttggggcagc ggccaatagc
     4381 agctttgctc cttcgctttc tgggctcaga ggctgggaag gggtgggtcc gggggcgggc
     4441 tcaggggcgg gctcaggggc ggggcgggcg cccgaaggtc ctccggaggc ccggcattct
     4501 gcacgcttca aaagcgcacg tctgccgcgc tgttctcctc ttcctcatct ccgggccttt
     4561 cgacctgcag ccaacgccac catggggacc gagtacaagc ccacggtgcg cctcgccacc
     4621 cgcgacgacg tcccccgggc cgtacgcacc ctcgccgccg cgttcgccga ctaccccgcc
     4681 acgcgccaca ccgtcgaccc ggaccgccac atcgagcggg tcaccgagct gcaagaactc
     4741 ttcctcacgc gcgtcgggct cgacatcggc aaggtgtggg tcgcggacga cggcgccgcg
     4801 gtggcggtct ggaccacgcc ggagagcgtc gaagcggggg cggtgttcgc cgagatcggc
     4861 ccgcgcatgg ccgagttgag cggttcccgg ctggccgcgc agcaacagat ggaaggcctc
     4921 ctggcgccgc accggcccaa ggagcccgcg tggttcctgg ccaccgtcgg cgtctcgccc
     4981 gaccaccagg gcaagggtct gggcagcgcc gtcgtgctcc ccggagtgga ggcggccgag
     5041 cgcgccgggg tgcccgcctt cctggagacc tccgcgcccc gcaacctccc cttctacgag
     5101 cggctcggct tcaccgtcac cgccgacgtc gaggtgcccg aaggaccgcg cacctggtgc
     5161 atgacccgca agcccggtgc catcgatgcc actaacttct ccctgttgaa acaagcaggg
     5221 gatgtcgaag agaatcccgg gccaatggtg agcaagggcg aggagctgtt caccggggtg
     5281 gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc acaagttcag cgtgtccggc
     5341 gagggcgagg gcgatgccac ctacggcaag ctgaccctga agttcatctg caccaccggc
     5401 aagctgcccg tgccctggcc caccctcgtg accaccctga cctacggcgt gcagtgcttc
     5461 agccgctacc ccgaccacat gaagcagcac gacttcttca agtccgccat gcccgaaggc
     5521 tacgtccagg agcgcaccat cttcttcaag gacgacggca actacaagac ccgcgccgag
     5581 gtgaagttcg agggcgacac cctggtgaac cgcatcgagc tgaagggcat cgacttcaag
     5641 gaggacggca acatcctggg gcacaagctg gagtacaact acaacagcca caacgtctat
     5701 atcatggccg acaagcagaa gaacggcatc aaggtgaact tcaagatccg ccacaacatc
     5761 gaggacggca gcgtgcagct cgccgaccac taccagcaga acacccccat cggcgacggc
     5821 cccgtgctgc tgcccgacaa ccactacctg agcacccagt ccgccctgag caaagacccc
     5881 aacgagaagc gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg gatcactctc
     5941 ggcatggacg agctgtacaa gtaattaatt aagctcgctg atcagcctcg actgtgcctt
     6001 ctagttgcca gccatctgtt gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg
     6061 ccactcccac tgtcctttcc taataaaatg aggaaattgc atcgcattgt ctgagtaggt
     6121 gtcattctat tctggggggt ggggtggggc aggacagcaa gggggaggat tgggaagaca
     6181 atagcaggca tgctggggat gcggtgggct ctatggcttc tgaggcgcgc cctcgaggtg
     6241 ccaccctcct cactccgtcc gcacctattt ccttttttca ttttttaaaa accatttaat
     6301 gccccgctct cagagctgga gggggcactc actgcatcac cagccagcag gggaagaaca
     6361 tgtccctgct gagctcccgt gccaggacga acttggaggt atgggctggg gctatgggga
     6421 aggaggaggc cccaggtcct gggcatgacc actaaaactg cactctgcca gggtcgggag
     6481 gcctggtggg actgccggcg gcgcggcagc cccaggaaat gccaggaccg aggatccccc
     6541 tggtgaaagg cgggtaggag gcgccataac ctccagccac cacacgcacg cacacagtcc
     6601 tggctgggga aagcccagac ccgtcacgtc tttgacaggc ctgaggcttg ctggttgcag
     6661 tggagttgtg aggctccagg tgagctcagc ttagctgcct gggatgccag c
//
